• Title/Summary/Keyword: Morphometric study

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Morphometrical Changes on Korean Rose Bitterling, Rhodeus uyekii, in Early Growth Period

  • Goo, In Bon;Lim, Sang Gu;Han, Hyung Kyun;Park, In-Seok
    • Development and Reproduction
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    • v.18 no.1
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    • pp.33-41
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    • 2014
  • We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea

  • Nam, Hajin;Kim, Yoo Yeon;Kim, Boyoung;Yoon, Won Kee;Kim, Hyoung-Chin;Suh, Jun Gyo
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.311-316
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    • 2018
  • Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y (ZfY) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus. To identify the genetic polymorphism in KWM/Hym, SNP analysis was performed. In a comparison with 28 SNP markers, there was a considerable difference between KWM/Hym and several inbred strains. The homogeneity between KWM/Hym and the inbred strains was as follows: C57BL/6J (39.3%), BALB/c AJic (42.9%), and DBA/2J (50%). KWM/Hym is most similar to the PWK/PhJ inbred strain (96.4%) derived from wild mice (Czech Republic). To identify the morphometric characteristics of KWM/Hym, the external morphology was measured. The tail ratio of male and female was $79.60{\pm}3.09$ and $73.55{\pm}6.14%$, respectively. KWM/Hym has short and agouticolored hairs and its belly is white with golden hair. Taking these results together, KWM/Hym, a newly developing inbred mouse originated from wild mouse, might be use as new genetic resources to overcome the limitations of the current laboratory mice.

Morphometric analysis of the inter-mastoid triangle for sex determination: Application of statistical shape analysis

  • Sobhani, Farshad;Salemi, Fatemeh;Miresmaeili, Amirfarhang;Farhadian, Maryam
    • Imaging Science in Dentistry
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    • v.51 no.2
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    • pp.167-174
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    • 2021
  • Purpose: Sex determination can be done by morphological analysis of different parts of the body. The mastoid region, with its anatomical location at the skull base, is ideal for sex identification. Statistical shape analysis provides a simultaneous comparison of geometric information on different shapes in terms of size and shape features. This study aimed to investigate the geometric morphometry of the inter-mastoid triangle as a tool for sex determination in the Iranian population. Materials and Methods: The coordinates of 5 landmarks on the mastoid process on the 80 cone-beam computed tomographic images(from individuals aged 17-70 years, 52.5% female) were registered and digitalized. The Cartesian x-y coordinates were acquired for all landmarks, and the shape information was extracted from the principal component scores of generalized Procrustes fit. The t-test was used to compare centroid size. Cross-validated discriminant analysis was used for sex determination. The significance level for all tests was set at 0.05. Results: There was a significant difference in the mastoid size and shape between males and females(P<0.05). The first 2 components of the Procrustes shape coordinates explained 91.3% of the shape variation between the sexes. The accuracy of the discriminant model for sex determination was 88.8%. Conclusion: The application of morphometric geometric techniques will significantly impact forensic studies by providing a comprehensive analysis of differences in biological forms. The results demonstrated that statistical shape analysis can be used as a powerful tool for sex determination based on a morphometric analysis of the inter-mastoid triangle.

Morphometric and Genetic Variability Among Tylenchulus semipenetrans Populations from Citrus Growing Area in Korea

  • Park, Byeong-Yong;Park, Sun-Nam;Lee, Jae-Kook;Bae, Chang-Hwan
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.236-240
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    • 2009
  • Tylenchulus semipenetrans, citrus nematode is an important phytopathogenic nematode and responsible for serious damage on citrus. However, little information is available about genetic variability of T. semipenetrans among different populations with variation of conventional diagnostic characteristics. In this study, we compared the morphometric and genetic characteristics among different populations. The mature female of T. semipenetrans collected in this study had thicker cuticle than those in the previous studies. In comparative sequence analysis of T. semipenetrans populations obtained from Jeju in Korea, we observed genetic variations within clones generated from single individuals. To determine whether variability among copies of nuclear ribosomal DNA sequences exists in the genome of T. semipenetrans, PCR-RFLP technique from individuals of Korean isolates with MseI and MspI restriction enzymes was used to prove experimentally that all populations have intra-specific variations. Restriction enzyme digestion created several fragments on 3.0% agarose gel corresponding to several haplotypes in all populations, though some populations displayed fragment deletion. The total length of fragments was larger than before digestion, indicating sequence heterogeneity within the genome of T. semipenetrans.

Morphometric Study of Achyranthes bidentata Complex Using Numerical Taxonomy (수리분류를 이용한 쇠무릎 분류군의 외부형태 연구)

  • Ahn, Young Sup;Kim, Kwan Su;Kim, Hui
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.6
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    • pp.466-471
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    • 2012
  • 'Usul' is a traditional medicinal herb, which has anti-inflammatory activities is distributed in India, Nepal, China, Korea, Japan. Korea pharmacopeia listed 'Usul' as either a species of Achyranthes japonica (Miq.) Nakai or A. bidentata Blume. Recent taxonomic studies in China and Japan delimited these taxa as two varieties, A. bidentata Blume var. bidentata and var. japonica Miq. A multivariate morphometric study of Achyranthes bidentata complex was undertaken to assess the entities of taxa that usefully could be recognized. Five quantitative characters were reviewed and analyzed with 293 specimens from Korea. The univariate analysis of inflorescence length, interval between florets, angle between floret and floral axis indicated that ranges among all taxa were continuous. However, quantitative characters of membrane size and the number of hairs within 4 were useful to identify two varieties. In PCA, the first three principal components accounted for 89.4% of the total variance. PCA revealed that var. bidentata showed distinctions in morphological attributes from var. japonica entity. Therefore, continued recognition at the infraspecifc level for these taxa is supported.

Morphometric Characteristics and Fin Dimorphism between Male and Female on the Marine Medaka, Oryzias dancena

  • Im, Jae Hyun;Gil, Hyun Woo;Lee, Tae Ho;Kong, Hee Jeong;Ahn, Cheol Min;Kim, Bong Seok;Kim, Dong Soo;Zhang, Chang Ik;Park, In-Seok
    • Development and Reproduction
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    • v.20 no.4
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    • pp.331-347
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    • 2016
  • Sexual dimorphism is the most conspicuous difference between the sexes. This study examines possible sexual dimorphism and the relative growth patterns of morphometric characteristics in the marine medaka, Oryzias dancena for their potential to help differentiate between males and females of this species. The von Bertalanffy growth parameters estimated by a non-linear regression method were $L_{\infty}=30.2mm$, K=3.22/year, and ${\tau}_0=-0.05$. All 18 characteristics measured showed a difference between males and females from 70 days after hatching. Each of these characteristics were significantly different between sexes (ANCOVA, P<0.05), and the ratio of standard length between sexes showed that males were larger than females for all five morphometric measurements. Fin length measurements were taken for 21 distances of anal fin and 7 distances of dorsal fin between landmarks. There were all differences for all dorsal fin rays between the males and the females and there is significant difference in 70 days after their hatch when the sexual dimorphism is presented. The significant difference (P<0.05) in fin ray for male and female was more greatly seen as they grow. Male marine medaka showed more rapid growth than females, with longer length, dorsal fins and anal fins. Differences in these characteristics will be useful during experiments when it is necessary to differentiate between sexes of marine medaka.

A study of trabecular bone strength and morphometric analysis of bone microstructure from digital radiographic image (디지털방사선영상에서 추출한 해면질골의 강도와 미세구조의 형태계측학적 분석에 대한 연구)

  • Han Seung-Yun;Lee Sun-Bok;Oh Sung-Ook;Heo Min-Suk;Lee Sam-Sun;Choi Soon-Chul;Park Tae-Won;Kim Jong-Dae
    • Imaging Science in Dentistry
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    • v.33 no.2
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    • pp.113-119
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    • 2003
  • Purpose : To evaluate the relationship between morphometric analysis of bone microstructure from digital radiographic image and trabecular bone strength. Materials and Methods : One hundred eleven bone specimens with 5 mm thickness were obtained from the mandibles of 5 pigs. Digital images of specimens were taken using a direct digital intraoral radiographic system. After selection of ROI (100 × 100 pixel) within the trabecular bone, mean gray level and standard deviation were obtained. Fractal dimension and the variants of morphometric analysis (trabecular area, periphery, length of skeletonized trabeculae, number of terminal point, number of branch point) were obtained from ROI. Punch sheer strength analysis was performed using Instron (model 4465, Instron Corp., USA). The loading force (loading speed 1 mm/min) was applied to ROI of bone specimen by a 2 mm diameter punch. Stress-deformation curve was obtained from the punch sheer strength analysis and maximum stress, yield stress, Young's modulus were measured. Results: Maximum stress had a negative linear correlation with mean gray level and fractal dimension significantly (p<0.05). Yield stress had a negative linear correlation with mean gray level, periphery, fractal dimension and the length of skeletonized trabeculae significantly (p < 0.05). Young's modulus had a negative linear correlation with mean gray level and fractal dimension significantly (p < 0.05). Conclusions : The strength of cancellous bone exhibited a significantly linear relationship between mean gray level, fractal dimension and morphometric analysis. The methods described above can be easily used to evaluate bone quality clinically.

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Hydraulic and Morphometric Characteristics of the Channel Bends (유로 만곡부의 수리 및 계량형태학적 특성)

  • Song, Jai Woo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.3
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    • pp.173-180
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    • 1992
  • A feature typical of natural rivers is the bend. The purpose of this study is to examine hydraulic and morphometric characteristics in channel bend reach by the deterministic approach. Cross section shape factor, "As" is suggested for a new cahracteristic factor of channel bend reach analysis. The variation of this new factor along the river reach showed the location of the concentration of the force due to the current all over the reach, that is curved or not. Some general meander factors are used for correlation with new factor suggested, and the applicability of "As" is verified. The range R/W values are concentrated 2~4, the meanning of this value can be regarded to the warning for bank erosion or breaking. And this paper dealt with prediction of cross section bed shape variation.

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