Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea

  • Nam, Hajin (Department of Medical Genetics, College of Medicine, Hallym University) ;
  • Kim, Yoo Yeon (Department of Medical Genetics, College of Medicine, Hallym University) ;
  • Kim, Boyoung (Department of Medical Genetics, College of Medicine, Hallym University) ;
  • Yoon, Won Kee (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Hyoung-Chin (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Suh, Jun Gyo (Department of Medical Genetics, College of Medicine, Hallym University)
  • Received : 2018.11.30
  • Accepted : 2018.12.19
  • Published : 2018.12.31
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Abstract

Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y (ZfY) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus. To identify the genetic polymorphism in KWM/Hym, SNP analysis was performed. In a comparison with 28 SNP markers, there was a considerable difference between KWM/Hym and several inbred strains. The homogeneity between KWM/Hym and the inbred strains was as follows: C57BL/6J (39.3%), BALB/c AJic (42.9%), and DBA/2J (50%). KWM/Hym is most similar to the PWK/PhJ inbred strain (96.4%) derived from wild mice (Czech Republic). To identify the morphometric characteristics of KWM/Hym, the external morphology was measured. The tail ratio of male and female was $79.60{\pm}3.09$ and $73.55{\pm}6.14%$, respectively. KWM/Hym has short and agouticolored hairs and its belly is white with golden hair. Taking these results together, KWM/Hym, a newly developing inbred mouse originated from wild mouse, might be use as new genetic resources to overcome the limitations of the current laboratory mice.

Keywords

Acknowledgement

Supported by : National Research Foundation

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