• 한국식품과학회지
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• 제42권5호
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• pp.571-577
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• 2010

본 연구에서는 예로부터 전해 내려오는 당뇨처방전에 사용되고 있는 문헌고찰을 토대로 6종의 약용식물(당귀, 백복령, 상백피, 상엽, 총목피 및 홍삼)의 항산화 및 항당뇨 활성을 비교 검토하고 이들의 상관관계를 검토하여 가장 효과적인 추출방법을 조사하였다. 당귀의 열수와 에탄올 추출물에서 각각 39.43와 37.75%로 다른 시료에 비하여 추출수율이 높았다. 폴리페놀 함량은 상백피 에탄올추출물에서 가장 높았고, 홍삼 초음파추출물에서 가장 낮았다. 플라보노이드 함량과 DPPH $RC_{50}$ 값은 상엽에탄올 추출물에서 가장 우수하였다. ${\alpha}$-Glucosidase저해활성은 상엽과 상백피 초음파추출물에서 높았고 추출방법 따른 저해율은 차이를 보였으나 세가지 추출물 모두 acarbose보다 높은 활성을 보였다. ${\alpha}$-Amylase저해활성은 상엽 에탄올추출물에서 가장 높은 저해활성을 보였고, 상백피와 백복령 에탄올추출물에서도 acarbose와 비슷한 결과를 보였다. 항산화 활성과 항당뇨 저해활성의 상관성은 에탄올 추출물과 초음파 추출물에서 유의적인 상관관계를 보였다. 에탄올 추출물에서 ${\alpha}$-glucosidase저해활성에 대한 폴리페놀 함량 또는 플라보노이드 함량과는 양의 상관관계($r^2$=0.792, 0.588)를 보였고, ${\alpha}$-amylase저해활성에 대한 플라보노이드 함량 또는 DPPH 소거능의 $RC_{50}$ 값에서도 음의 상관관계($r^2$=0.918, -0.594)를 나타내었다. 또한 ${\alpha}$-amylase 저해활성과 ${\alpha}$-glucosidase저해활성에서는 양의 상관관계($r^2$=0.746)를 보였다. 초음파 추출물에서는 ${\alpha}$-glucosidase저해활성에 대해 폴리페놀 함량과 플라보노이드 함량에서만 양의 상관관계($r^2$=0.763, 0.618)를 보였다. 항산화능에서의 상관관계를 살펴 본 결과 열수, 에탄올 및 초음파추출물에서 모두 폴리페놀 함량과 플라보노이드 함량, DPPH $RC_{50}$ 값과 유의적으로 높은 양의 상관관계가 있는 것으로 나타났다. 결론적으로 약용식물이 추출방법에 따른 항산화, 항당뇨 활성과 상관성에 차이를 보이고 있음을 알 수 있었다.

• 한국식품저장유통학회지
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• 제18권6호
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• pp.844-852
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• 2011

본 연구는 누에, 상심자, 상엽, 상지, 산약 및 차가버섯을 편리하게 음용할 수 있도록 제품을 개발하기 위하여 혼합 추출물의 성분과 기능성을 분석한 후 음료를 제조하여 음료의 성분 분석, 기능성 평가 및 동물실험을 수행하였다. 혼합 추출물의 일반성분 분석 결과 조단백질 0.31%, 조지방 0.38%, 탄수화물 0.42% 및 조회분 0.19%이었다. 유리당은 fructose 0.067 g/100 mL, glucose 0.047 g/100 mL이었고 DNJ는 161.02 mg/gdw을 함유하고 있었으며 항산화성을 검토한 결과 모든 항목에서 우수한 결과를 나타내었다. 또한 ${\alpha}$-glucosidase 저해활성도 우수하였다. 혼합 추출물을 이용하여 제조한 음료의 일반성분을 분석한 결과 조단백질 0.42%, 조지방 0.31 및 탄수화물 9.9%로 나타났다. 음료의 유리당 함량 분석 결과 fructose와 glucose가 증가하였다. 음료의 기능성을 평가한 결과 total polyphenol 함량은 71.93%이었고 전자공여능은 농도 5%에서 가장 높았으며 TBARS를 실험한 결과 특히 $KO_2$에 대한 억제능이 높은 것으로 나타났다. Streptozotocin으로 당뇨를 유발한 후 5주간 음료를 공급하여 혈당의 변화를 측정한 결과 실험 기간 중 정상군(A)은 혈당변화가 거의 없었으며, 당뇨 대조군(B)은 2주째부터 5주차까지 비슷하게 경향의 높은 혈당치를 나타내었다. 음료 경구 투여군(C)에서는 2주차부터 당뇨 대조군(B)에 비해 낮은 혈당치를 나타내었다. 음료 자유급여군(D)은 2주차부터 혈당치가 완만하게 떨어져 5주차에서는 당뇨 유발시험물질 투여군 중 가장 낮은 혈당치를 나타내는 경향을 보였다.

• 약학회지
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• 제47권1호
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• pp.14-19
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• 2003

The hair cycle consists of three phases, growth (anagen), involution (catagen) and quiescence (telogen) phases. In order to evaluate hair re-growth effect of herbal extracts mixture containing the 70％ ethanol extracts of Polygoni Multiflori Radix, Mori Cortex Radicis, Gingko Biloba Folium and Pine bud, we have examined the induction of the anagen phase and/or elongation of the anagen period using C3H mice. Morphological examination was done by Hattori' and Ogawa's method. Enzyme activities of ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GT) and alkaline phosphatase (ALP) was detected by Bessey-Lovry-Brock's method. Enzyme activity as a biochemical marker of hair cycle was investigated in the third hair cycle period of C3H mice after depilation. 3％ Minoxidil treated group and herbal extract mixture treated group were shown 3 days earlier initiation of anagen than control group. In cycling mouse skin, ${\gamma}$-GT activity is pronounced during anagen and greatly diminished during telogen. Herbal extract mixture has shown promising hair re-growth effect on hair follicular cycles of C3H mice.

• 대한화장품학회지
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• 제40권1호
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• pp.95-108
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• 2014

화장품은 일반인이 장기간 연용하는 제품으로 인체 피부에 대한 안전성이 매우 중요하다. 화장품에 의한 접촉피부염은 자극성 물질이 피부에 접촉 후 침투하여 유발하는 염증반응으로 활성화된 면역세포에서 염증을 매개하는 다양한 인자를 분비함으로써 시작된다. 본 연구에서는 국산 생약 추출물을 이용하여 RAW264.7 대식 세포에서 염증 관련 인자에 대한 영향을 통해 항염증 활성을 스크리닝 하였다. 51종의 국산 생약 추출물 중 측백, 측백엽(초), 향부자, 형개, 동과자, 산약, 산약(초), 상지, 송절, 택사의 에탄올 추출물 10종이 lipopolysaccharide(LPS)에 의해 유도된 세포독성을 감소시킴과 동시에 염증 관련 인자인 Nitric oxide (NO), interleukin (IL)-1${\beta}$, IL-6, tumor necrosis factor (TNF)-${\alpha}$의 생성을 억제하는 것을 확인하였다. 또한, 이들 생약 추출물은 인체피부에서도 자극을 유발하지 않음을 인체 첩포시험을 통해 검증하였다. 따라서, 항염증 활성이 확인된 10종 국산 생약 추출물은 피부 자극을 예방할 수 있는 화장품 소재로서의 활용이 가능할 것으로 사료된다.

• 대한한의학방제학회지
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• 제20권1호
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• pp.123-132
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• 2012

Objectives : The purpose of this study is to find out herb-pair prototype composed of Zanthoxyli Fructus, to examine compatibility regulation between them and to classify them into seven features of compatibility. Methods : To search usages of Zanthoxyli Fructus compatibility, we have investigated the classics of herb and prescription such as "五十二病方"(Fifty-two kinds of Diseases), "黃帝內經"(The Medical Classic of Yellow Emperor), "傷寒論"(Treatise on Febrile Caused by Cold), "金匱要略"(Synopsis of Prescriptions of The Golden), "附廣肘后方"(Handbook of Prescriptions for Emergencies), "本草綱目"(Compendium of Materia Medica), "得配本草"(Deukbae Materia Medica). Results : In these books Zanthoxyli Fructus was paired with below herbs; 桂(Cinnamomi Cortex), 薑(Zingiberis Rhizoma), 吳茱萸(Evodiae Fructus), 細辛(Asari Radix), 茴香(Foeniculi Fructus), 肉荳蔲(Cistanches Herba), 釜下土(Terba Flava Usta), 益智仁(Alpinae Oxyphyllae Fructus), 飴(Saccharum Granorum), 葱白(Allii Radix), 梨(Pyrus), 牙皂(Gleditsia Fructus), 杏仁(Armeniacae Amarum Semen), 蕎麥粉(Fagopyrum esculentum Moench), 胡粉(Minium), 白朮(Atractylodis Rhizoma White), 蒼朮(Atractylodis Rhizoma), 麻子仁(Cannabis Fructus), 大麥蘗(Hordei Fructus Germiniatus), 麴(Massa Medicata Fermentata), 生地黃(Rehmanniae Radix), 茯苓(Poria), 牡蛎(Ostreae Concha), 白礬(Alumen), 雄黃(Realgar), 烏頭(Aconiti Radix), 附子(Aconiti Lateralis Preparata Radix). Conclusions : Zanthoxyli Fructus herb-pair classified by seven features of compatibility are as follows; 1. Mutual reinforcement compatibility with : Cinnamomi Cortex, Zingiberis Rhizoma, Evodiae Fructus, Asari Radix, Foeniculi Fructus, Cistanches Herba, Terba Flava Usta, Alpinae Oxyphyllae Fructus, Saccharum Granorum, Allii Radix, Pyrus, Gleditsia Fructus. 2. Mutual assistance compatibility with : Armeniacae Amarum Semen, Fagopyrum esculentum Moench, Minium, Atractylodis Rhizoma White, Atractylodis Rhizoma, Cannabis Fructus, Hordei Fructus Germiniatus, Massa Medicata Fermentata, Rehmanniae Radix, Poria, Ostreae Concha, Alumen. 3. Mutual restraint compatibility with: Realgar, Aconiti Radix, Aconiti Lateralis Preparata Radix) 4. Mutual detoxication compatibility with: 桑汁(Juice of Mori Folium), 桑根汁(Juice of Morus Cortex), 大蒜(Allii Bulbus), 薺苨(Remotiflori Radix), 桂汁(Juice of Cinnamomi Cortex).

• 한국식품영양과학회지
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• 제32권4호
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• pp.614-620
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• 2003

상엽과 상심자, 구기자, 인삼 등은 당뇨에 효능이 있는 것으로 널리 알려진 약재들이다. 본 실험에서는 기본 실험을 통하여 상엽, 상심자, 구기자, 인삼, 차가버섯의 최적 배합비와 최적의 추출방법으로 엑기스 형태로 만들어진 소당엑스$^{TM}$와 이를 특허 출원한 방법으로 백미에 코팅한 소당미$^{TM}$를 사용하여 시험한 결과 Alloxan으로 당뇨를 유도한 마우스, 정상인, 그리고 당뇨환자 모두에서 혈당이 저하되었음을 확인하였다. 따라서 소당엑스$^{TM}$와 소당미$^{TM}$는 당뇨환자의 혈당 조직 및 합병증 예방에 효과적이므로 당뇨식이요법제로서 이용될 수 있는 건강 식품으로 사료된다.

• 생약학회지
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• 제30권2호
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• pp.123-129
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• 1999

The hypoglycemic effect of mulberry leaves using alloxan-induced diabetic mice was investigated. The hypoglycemic effect of mulberry leaves was significantly affected with anaerobic incubation up to for 6 hrs. The body weight of mice treated with 20% anaerobic conditioned mulberry leaves was higher than that in the control group. Mulberry leaves which were treated with anaerobic condition significantly decreased blood glucose level in alloxan-induced type I diabetes mice. Furthermore, glucose tolerance was significantly recovered by feeding mulberry leaves anaerobically treated. Pathological analysis revealed that the disappearance of insulin-secreting beta-cells in the islets of Langerhans from the alloxan induced diabetic mice was strongly inhibited in the mulberry leaves feeding group. The content of 1-deoxynojirimycin increased up to 5% in detached mulberry leaves incubated under nitrogen gas for 6 hrs. Nevertheless, it is not clear whether the synthesis of 1-deoxynojirimycin is increased or the degradation of it is decreased. Overall, results obtained from this study indicate that the hypoglycemic effect was increased by the mulberry leaves with anaerobic treatment.

• 동국한의학연구소논문집
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• 제4권
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• pp.267-311
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• 1995

This study was performed to analyze the effects of WhoaDam-JiHae PyeongChunYak on contracted tracheal smooth muscle in rat. Transverse strips were used for the experiment using organ bath. The test strip was perfused with modified Krebs-Ringer Bicarbonate solution which was aerated with 95% $O_2$-5% $CO_2$ mixed gas and kept at $37^{\circ}C$. WhoaDam-JiHae PyeongChunYak extract and acetylcholine infused tracheal strip that was contracted with acetylcholine. The results were as follows : 1. The contractile force of tracheal strip by acetylcholine was significantly increased by Pinelliae Rhizoma, Arisaematis Rhizoma, Sinapis Semen. Typhonii Rhizoma, Peucedani Radix, Eriobotryae Folium, Platycodi Radix, Bambusae Caulis Taeniam, Benincasae Semen, Armeniacae Amarum Semen, Asteris Radix, Periliae Fructus, Mori cortex and Lepidii Semen. 2. Arisaematis Rhizoma($1{\mu}{\ell}/m{\ell}$, $3{\mu}{\ell}/m{\ell}$, $10{\mu}{\ell}/m{\ell}$) and Asteris Radix($1{\mu}{\ell}/m{\ell}$, $3{\mu}{\ell}/m{\ell}$) slightly relaxed the contracted tracheal strip by acetylcholine. 3. Farfarae Flos significantly relaxed the contracted tracheal strip by acetylcholine. 4. The contractile force of tracheal strip by acetylcholine was significantly increased by Stemonae Radix at $30{\mu}{\ell}/m{\ell}$, on the other hand Stemonae Radix at $100{\mu}{\ell}/m{\ell}$ significantly relaxed the contracted tracheal strip by acetylcholine.

• 대한한의학회지
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• 제17권1호
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• pp.222-233
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• 1996

In order to understand the stimulation quality of Artemisine Vulgaris Folium(Bong), Mori Ramulus(Sangi) and Persicae Ramulus(Dogi) combustion, and get the basic data for the development of electric moxibustion apparatus, the average temperature, peak temperature, average gradient temperature, maximum gradient temperature and combustion time of heating period on the three moxa materials were measured. 1. The average combustion temperature was high in order of Dogi, Sangi and Bong, and these were acknowledged to have a significant difference each other in the average temperature. 2. The peak combustion temperature among those was high in order of Dogi, Sangi and Bong, and Bong was acknowledged to have significant differences with Dogi and Sangi. Sangi and Dogi however were not acknowledged to have difference each other. 3. The average gradient temperature among those was high in order of Dogi, Sangi and Bong, and Bong was proved to have significant differences with Dogi and Sangi. Sangi and Dogi however were not proved to have difference each other. 4. The maximum temperature among those was high in order of Dogi, Sangi and Bong, bong was acknowledged to have signigicant differences with Dogi and Sangi, but Sangi and Dogi were not proved to have difference each other. 5. The combustion time was short in order of Dogi, Sangi and Bong. Bong was acknowledged to have significant differences with Dogi and Sangi, but Sangi and Dogi were not. In order to understand well the characteristics of combustion, it is required to have a quantitative interpretation of combustion calory, and, in the future, we expect it is required to have a consistent study for the clinical effectiveness and the mutual relationship according to the combustion characteristics.

• 동아시아식생활학회지
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• 제14권5호
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• pp.425-437
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• 2004

Medicinal plants are of great importance in providing healthcare to a large portion of the population in Korea. A number of plants are described in Dong-Ui-Bo-Gam for use in the treatment of allergic disorders, namely psoriasis, eczema, bronchial asthma, etc. In this study, we evaluated the effect of AllerQ, which is multi-complexes of various plants extracts such like Mori folium, Scutellaria baicallensis, Glycyrrhiza uralnsis, Mentha sacharinensis and Poncirus trifoliata on compound 48/80 induced anaphylactic shock, ovalbumin induced asthma in vivo and anti-IgE antibody induced hypersensitivity in vitro. We found antianaphylactic or antiallergic properties of AllerQ when given orally. AllerQ for prophylactic treatment for anaphylactic shocks have produced good results. AllerQ may modulate various aspects of immune function and allergic inflammation. In the present study, we analyse the effects of AllerQ on mast cell degranulation, mortality, cAMP/cGMP, O₂, H₂O₂ level, cyokine production and on the elicitation of IgE-mediated mast cell-dependent allergic inflammation in vivo and in vitro. We have established that AllerQ inhibited histamine release, cAMP/cGMP, O₂, H₂O₂ level, IL-4, tumor necrosis factor-alpha(TNF-α) and IL-6 production without having any significant physical change. These effects have been observed in mast cell(in vitro) and serum(in vivo) derived from three different origins that were activated by either immunological or non-immunological stimuli. These results suggest that the antianaphylactic and antiasthma tic action of AllerQ may be associated with an increase in the intracellular inhibition of the cAMP phosphodiesterase. Furthermore, AllerQ identified as potent inhibitors on O₂, H₂O₂ and cytokine activity. these data suggest that AllerQ may have an inhibitory role in mast cell-mediated allergic inflammation, and thus might be considered as an useful functional food.