• Ocean and Polar Research
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• 제34권1호
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• pp.85-91
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• 2012

Physical properties such as sound speed contrast (h) and density contrast (g) of the interested target are key parameters to understand acoustic characteristics by using theoretical scattering models. The density and sound speed of moon jellyfish (common jellyfish, Aurelia aurita s.l.) were measured. Sound speed contrast (h) was measured from travel time difference (time-of-flight method) of an acoustic signal in a water tank for APOP studies (Acoustic Properties Of zooplankton). Density contrast (g) was measured by the displacement volume and wet weight (dual-density method). The sound speed remained almost constant as the moon jellyfish increased in bell length. The mean values${\pm}$standard deviation of h and g were $1.0005{\pm}0.0012$ and $0.9808{\pm}0.0195$), respectively. These results will provide important input for use in theoretical scattering models for estimating the acoustic target strength of jellyfish.

• Fisheries and Aquatic Sciences
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• 제14권3호
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• pp.174-178
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• 2011

This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme, Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an $IC_{50}$ value of 3.8 ${\mu}g$/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.

• 한국해양학회지:바다
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• 제8권2호
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• pp.199-202
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• 2003

하계 한국남해안에서 보름달물해파리(Scyphozoa: Ulmariidae: Aurelia aurita)의 월별 출현 및 크기와 중량간의 상관성을 연구하였다. 또한 Rotifer와 Artemia sp.를 먹이생물로 하여 먹이섭취 습성을 분석하였다. 보름달물해파리는 크기와 중량간에 상관성이 매우 높았으며 (r=0.930, P<0.001), 6월에는 크기가 6∼9 cm, 7월에는 7∼16 cm, 8월에는 16 cm인 개체가 우점하였다. 먹이섭취율은 크기와 먹이농도에 따라 유의한 차이가 있었다(ANOVA test: P<0.001).

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.283-288
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• 2011

The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using $C_{18}$ and $C_{12}$ columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The $IC_{50}$ and $K_i$ values were $6.56{\pm}1.12$ and $3.10{\pm}0.28\;{\mu}M$, respectively, indicating competitive inhibition of angiotensin-I-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.

• 수산해양기술연구
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• 제49권4호
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• pp.483-491
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• 2013

The sound-speed and density contrasts are important factors in estimating the target strength (TS) of moon jellyfish (Aurelia aurita). In this study, the sound-speed and density contrasts were measured using time-of-flight and neutral buoyancy methods, respectively. The sound-speed contrast of A. aurita was from 0.9966 to 1.0031 (mean${\pm}$SD, $0.9999{\pm}0.0017$) and no distinct differences in temperature or pulsation activity and weak were found. The density contrast was from 0.9994 to 1.0004 (mean${\pm}$SD, $1.0000{\pm}0.0002$). The density of A. aurita was substantially different but the density contrast of A. aurita was shown to be similar to that in the sampling location. The results can be used to estimate of TS of A. aurita by acoustic model.

• 한국수산과학회지
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• 제44권5호
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• pp.478-483
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• 2011

An antimicrobial material was purified from the acidified whole body extract of the jellyfish Nemopilema nomurai by using C18 reversed phase and cation-exchange high performance liquid chromatography (HPLC). Whole body extract and the purified compound (JAP-1) showed potent antimicrobial activities against a wide range of microorganisms including Escherichia coli D31, Bacillus subtilis, Streptococcus iniae and Candida albicans, without significant hemolytic activity. Treatment of JAP-1 with trypsin completely abolished all antibacterial activity against Bacillus subtilis, suggesting that JAP-1 is likely to be a proteinaceous antibiotic. The molecular weight of JAP-1 was determined to be 680.10 Da by MALDI-TOF mass spectroscopy.

• 한국수산과학회지
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• 제52권6호
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• pp.725-734
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• 2019

Moon jellyfish Aurelia coerulea are highly abundant off the coast of Tongyeong, Korea. We measured the density of A. coerulea in this area using a scientific echosounder at frequencies of 38 and 120 kHz, and then applied a distorted wave Born approximation (DWBA) model to calculate the target strength of the echosounder at each frequency. Then, we used the frequency difference method to extract jellyfish echo signals and estimate the A. coerulea density. A. coerulea was evenly distributed throughout the water column; the backscattering strength ranged from -75 to -65 dB. In May and August, the A. coerulea densities at survey lines 3 and 4 were estimated at 1.5-1.6 and 0.2-0.9 g/m2, with mean weighted densities of 1.04 and 0.48 g/m2, respectively. In September, the A. coerulea densities estimates in Jaran Bay and Goseong Bay were 0.6-2.1 and 0.1-0.4 g/m2, with mean weighted densities of 1.25 and 0.24 g/m2, respectively.

• 한국해양학회지:바다
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• 제20권1호
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• pp.36-42
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• 2015

해파리에 대한 쥐치의 포식률을 파악하기 위하여 메두사, 에피라, 폴립 단계의 보름달물해파리(Aurelia aurita s.l.)를 쥐치(Stephanolepis cirrhifer)에게 공급하고 포식실험을 수행하였다. 모든 크기의 쥐치는 메두사를 적극적으로 포식하였으며, 포식률은 쥐치의 체중과 비례하였다. 평균 습중량 0.5, 1, 2.2 g의 쥐치 치어는 평균 $0.77{\pm}0.51g\;fish^{-1}h^{-1}$을 포식하였으며, 평균 습중량 150, 344 g의 쥐치 성어는 각각 $9.26{\pm}1.61$, $15.89{\pm}3.67g\;fish^{-1}h^{-1}$을 포식하였다. 유생에 있어서도 평균 습중량 3.7 g의 쥐치가 10분 이내에 100개체의 에피라를 포식하였고, 평균 습중량 2.2 g의 쥐치는 단위체중당 $5.73{\pm}0.59$ polyps $g^{-1}$ of fish $h^{-1}$의 폴립을 포식하였다. 반면에 쥐치 성어는 폴립을 포식하지 않았다. 이와 같은 쥐치의 해파리 포식이 현장에서도 가능한지 알아보기 위해 수행된 먹이 선호도 실험에서, 쥐치 성어는 다른 먹이인 굴(약 60%)과 갯지렁이(약 80%)를 주로 포식하였고 메두사 포식은 약 2%에 불과했다. 하지만 쥐치 치어는 다른 먹이인 곤쟁이류와 함께 에피라, 폴립도 포식하였으며, 폴립에 대해서는 지속적인 포식활동을 보였다. 그리고 쥐치 치어는 주변에 다른 먹이가 있어도 보름달물해파리 유생에 대한 포식이 활발했지만, $11^{\circ}C$ 이하에서는 섭식활동을 중지한 실험결과로부터 $11^{\circ}C$ 보다 수온이 낮은 3-4월경에 발생하는 에피라 유생에 대한 현장에서의 포식 가능성은 낮을 것으로 예상할 수 있다. 반면, 폴립의 경우 수온에 관계없이 연중 서식하기 때문에 현장에서 쥐치 치어의 폴립 포식이 가능할 것이다. 따라서 쥐치를 이용하여 보름달물해파리 구제작업을 시행할 경우, 폴립을 대상으로 하여 쥐치 치어를 이용하는 것이 가장 효과적일 것으로 판단된다.

• 한국지리정보학회지
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• 제9권1호
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• pp.168-178
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• 2006

최근 울진원자력 발전소에 대량의 해파리가 유입되어 냉각용수 공급을 위한 취수구를 막고 발전장애를 일으키는데, 이에 대한 사전 방지 대책 중 하나로 해양생물 대량유입의 감시 및 예보의 필요성이 제기되고 있다. 자체 이동 능력이 약한 보름달 물해파리와 같은 해양생물체에 대해서는 해수유동이 중요한 유입 요소로 작용하며, 울진주변해역에서 표층 해류방향이 북서향일 때에는 대마난류를 타고 올라온 해파리가 발전소 쪽으로 이동하여 피해를 주게 된다. 난류수의 유동방향 탐지를 위해 울진해역 $25km{\times}25km$ 공간범위를 설정하여 NOAA 해수면온도 관측자료를 수집하였다. 통계적 분석을 위해 수온자료는 GIS point 데이터로 만들고 ArcGIS의 geostatistical analyst를 사용하였으며, 수온 point 데이터에 대해 방향성 반베리오그램(directional semivariogram) 계산과 이방성(anisotropy)으로 해수면 난류수 유동방향을 탐지하였다. 이러한 실험적 결과는 발전소 취수구에 해양생물 대량유입을 감시하는 요소기술로 해파리유입 조기경보시스템 개발에 활용될 수 있을 것으로 기대한다.

• 수산해양기술연구
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• 제51권3호
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• pp.295-301
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• 2015

Target strength (TS) information is an important parameter that estimates the detection, distribution, and abundance of Aurelia aurita. In order to investigate the biological TS of jellyfish, some factors such as size (bell diameter), tilt angle, pulsation, and symbion should be known. In the ex situ TS measurements, the tilt angles and pulsation from synchronized swimming behavior of four live A. aurita (bell diameters in the air: 54.2 ~ 94.2 mm) were measured with the acoustic data at 70 kHz. The reduced target strength (RTS) of A. aurita was found to change ranged from 13.4 ~ 16.5 dB according to the incidence angles from $-30^{\circ}$ to $24^{\circ}$. When the change rate of bell diameter in the water was 0.2, the TS value showed a 7.2 dB change. These results could be utilized as an important data to understand the acoustic characteristic scattering of A. aurita.