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http://dx.doi.org/10.5657/kfas.2011.0478

Isolation and Purification of an Antimicrobial Material from the Jellyfish Nemopilema nomurai  

Moon, Ho-Sung (Food Safety Research Division, National Fisheries Research and Development Institute)
Kim, Yeon-Kye (Food Safety Research Division, National Fisheries Research and Development Institute)
Lee, Moon-Hee (Food Safety Research Division, National Fisheries Research and Development Institute)
Yoon, Na-Young (Food Safety Research Division, National Fisheries Research and Development Institute)
Lee, Doo-Seog (Food Safety Research Division, National Fisheries Research and Development Institute)
Yoon, Ho-Dong (Food Safety Research Division, National Fisheries Research and Development Institute)
Seo, Jung-Kil (Department of Biotechnology, College of Fisheries Science, Pukyong National University)
Park, Nam-Gyu (Department of Biotechnology, College of Fisheries Science, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.44, no.5, 2011 , pp. 478-483 More about this Journal
Abstract
An antimicrobial material was purified from the acidified whole body extract of the jellyfish Nemopilema nomurai by using C18 reversed phase and cation-exchange high performance liquid chromatography (HPLC). Whole body extract and the purified compound (JAP-1) showed potent antimicrobial activities against a wide range of microorganisms including Escherichia coli D31, Bacillus subtilis, Streptococcus iniae and Candida albicans, without significant hemolytic activity. Treatment of JAP-1 with trypsin completely abolished all antibacterial activity against Bacillus subtilis, suggesting that JAP-1 is likely to be a proteinaceous antibiotic. The molecular weight of JAP-1 was determined to be 680.10 Da by MALDI-TOF mass spectroscopy.
Keywords
Jellyfish; Nemopilema nomurai; Antimicrobial material; HPLC; Hemolysis;
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