• Ocean and Polar Research
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• v.34 no.1
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• pp.85-91
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• 2012

Physical properties such as sound speed contrast (h) and density contrast (g) of the interested target are key parameters to understand acoustic characteristics by using theoretical scattering models. The density and sound speed of moon jellyfish (common jellyfish, Aurelia aurita s.l.) were measured. Sound speed contrast (h) was measured from travel time difference (time-of-flight method) of an acoustic signal in a water tank for APOP studies (Acoustic Properties Of zooplankton). Density contrast (g) was measured by the displacement volume and wet weight (dual-density method). The sound speed remained almost constant as the moon jellyfish increased in bell length. The mean values${\pm}$standard deviation of h and g were $1.0005{\pm}0.0012$ and $0.9808{\pm}0.0195$), respectively. These results will provide important input for use in theoretical scattering models for estimating the acoustic target strength of jellyfish.

• Fisheries and Aquatic Sciences
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• v.14 no.3
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• pp.174-178
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• 2011

This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme, Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an $IC_{50}$ value of 3.8 ${\mu}g$/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.199-202
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• 2003

Monthly occurrence of the moon jellyfish (Scyphozoa: Ulmariidae: Aurelia aurita) was investigated in the southern coast of Korea in summer. The relationships not only between weight and length but also between length and food (rotifer and Artemia sp.) ingestion were also studied. Aurelia was monthly sampled 5 or 6 times at the coastal area between Jinhae and Geoje-do with a landing net (mouth: 30 cm, mesh size: 2 cm) at surface from June to August,2001. Aurelia aurita was dominated by 6-9 cm in June, 7-16 cm in July and 16 cm in August in bell diameter. The bell diameter was significantly related with weight (r=0.930, P<0.001). The ingestion rate was significantly different according to bell diameter and food density (ANOVA test: P<0.001).

• Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.283-288
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• 2011

The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using $C_{18}$ and $C_{12}$ columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The $IC_{50}$ and $K_i$ values were $6.56{\pm}1.12$ and $3.10{\pm}0.28\;{\mu}M$, respectively, indicating competitive inhibition of angiotensin-I-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.49 no.4
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• pp.483-491
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• 2013

The sound-speed and density contrasts are important factors in estimating the target strength (TS) of moon jellyfish (Aurelia aurita). In this study, the sound-speed and density contrasts were measured using time-of-flight and neutral buoyancy methods, respectively. The sound-speed contrast of A. aurita was from 0.9966 to 1.0031 (mean${\pm}$SD, $0.9999{\pm}0.0017$) and no distinct differences in temperature or pulsation activity and weak were found. The density contrast was from 0.9994 to 1.0004 (mean${\pm}$SD, $1.0000{\pm}0.0002$). The density of A. aurita was substantially different but the density contrast of A. aurita was shown to be similar to that in the sampling location. The results can be used to estimate of TS of A. aurita by acoustic model.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.478-483
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• 2011

An antimicrobial material was purified from the acidified whole body extract of the jellyfish Nemopilema nomurai by using C18 reversed phase and cation-exchange high performance liquid chromatography (HPLC). Whole body extract and the purified compound (JAP-1) showed potent antimicrobial activities against a wide range of microorganisms including Escherichia coli D31, Bacillus subtilis, Streptococcus iniae and Candida albicans, without significant hemolytic activity. Treatment of JAP-1 with trypsin completely abolished all antibacterial activity against Bacillus subtilis, suggesting that JAP-1 is likely to be a proteinaceous antibiotic. The molecular weight of JAP-1 was determined to be 680.10 Da by MALDI-TOF mass spectroscopy.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.6
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• pp.725-734
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• 2019

Moon jellyfish Aurelia coerulea are highly abundant off the coast of Tongyeong, Korea. We measured the density of A. coerulea in this area using a scientific echosounder at frequencies of 38 and 120 kHz, and then applied a distorted wave Born approximation (DWBA) model to calculate the target strength of the echosounder at each frequency. Then, we used the frequency difference method to extract jellyfish echo signals and estimate the A. coerulea density. A. coerulea was evenly distributed throughout the water column; the backscattering strength ranged from -75 to -65 dB. In May and August, the A. coerulea densities at survey lines 3 and 4 were estimated at 1.5-1.6 and 0.2-0.9 g/m2, with mean weighted densities of 1.04 and 0.48 g/m2, respectively. In September, the A. coerulea densities estimates in Jaran Bay and Goseong Bay were 0.6-2.1 and 0.1-0.4 g/m2, with mean weighted densities of 1.25 and 0.24 g/m2, respectively.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.1
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• pp.36-42
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• 2015

To determine the predation rate of threadsail filefish, we conducted feeding experiment of threadsail filefish, Stephanolepis cirrhifer on different life stages (medusae, ephyrae, and polyps) of Aurelia aurita s.l. in the laboratory. All size groups of threadsail filefish actively preyed on moon jellyfish Aurelia aurita s.l. and predation rate was proportional to the wet weight of threadsail filefish. Average wet weight 0.5, 1, 2.2 g of threadsail filefish preyed on average $0.77{\pm}0.51g\;fish^{-1}h^{-1}$ of medusae. Average wet weight 150 and 344 g of threadsail filefish preyed on $9.26{\pm}1.61$ and $15.89{\pm}3.67g\;fish^{-1}h^{-1}$ of medusae respectively. In ephyrae and polyps, average wet weight 3.7 g of juvenile of threadsail filefish preyed on 100 ephyrae within 10 minutes and average wet weight 2.2 g of juvenile of threadsail filefish preyed on $5.73{\pm}0.59$ polyps $g^{-1}$ of fish $h^{-1}$. Whereas adults of threadsail filefish did not prey on polyps. In feeding preference experiments to elucidate whether threadsail filefish prey on moon jellyfish in the sea, adults of threadsail filefish preyed on mostly oysters (ca. 60%) and polychaetes (ca. 80%). Medusae were consumed only 2% of total. However, juvenile of threadsail filefish preyed on ephyrae and polyps as well as mysid shrimps. Besides, polyps were consistently consumed during the experiment. Although juvenile of threadsail filefish consumed all kinds of prey, they ceased feeding at temperatures below $11^{\circ}C$, which suggests that possibility of predation on ephyrae may be very low in the sea because ephyrae are released at low temperatures below $11^{\circ}C$ from March to April. Whereas polyps are inhabitable in all seasons in Korean coastal waters, juvenile of threadsail filefish may prey on polyps in the sea. These results suggest that removing moon jellyfish at polyp stage by using juvenile of threadsail filefish is the most effective way for extermination of moon jellyfish.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.1
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• pp.168-178
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• 2006

In recent years, an ingress of mass jellyfish into cooling water intake system causes interruption of electric power production at the Uljin nuclear power plant. Therefore, monitering and forecast on the mass ingress of marine organisms are demanded as one of the early preventing measurements. Sea water movement is a major factor on the ingress of marine organisms like Moon jellyfish which has weak self-mobile ability. When sea surface flow direction adjacent to the Uljin is the northwest, the jellyfish on the Tsushima warm currents move to the Uljin power plant. To detect the direction of sea surface warm flows, the spatial range with $25km{\times}25km$ is set up and NOAA sea surface temperature(SST) data are collected in this area. For the statistical analysis, the SST data are made as GIS point data and geostatistical analysis of ArcGIS is used. Analyzing directional semivariogram, the anisotropy of the SST point data are calculated and warm flow direction is detected. This experimental results are expected to use as an element technology for the early warning system development of mass jellyfish ingress in power plant.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.3
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• pp.295-301
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• 2015

Target strength (TS) information is an important parameter that estimates the detection, distribution, and abundance of Aurelia aurita. In order to investigate the biological TS of jellyfish, some factors such as size (bell diameter), tilt angle, pulsation, and symbion should be known. In the ex situ TS measurements, the tilt angles and pulsation from synchronized swimming behavior of four live A. aurita (bell diameters in the air: 54.2 ~ 94.2 mm) were measured with the acoustic data at 70 kHz. The reduced target strength (RTS) of A. aurita was found to change ranged from 13.4 ~ 16.5 dB according to the incidence angles from $-30^{\circ}$ to $24^{\circ}$. When the change rate of bell diameter in the water was 0.2, the TS value showed a 7.2 dB change. These results could be utilized as an important data to understand the acoustic characteristic scattering of A. aurita.