• Mycobiology
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• 제46권2호
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• pp.114-121
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• 2018

Mon1 is a guanine nucleotide exchange factor subunit that activates the Ypt7 Rab GTPase and is essential for vacuole trafficking and autophagy in eukaryotic organisms. Here, we identified and characterized the function of Mon1, an ortholog of Saccharomyces cerevisiae Mon1, in a human fungal pathogen, Cryptococcus neoformans. Mutation in mon1 resulted in hypersensitivity to thermal stress. The mon1 deletion mutant exhibited increased sensitivity to cell wall and endoplasmic reticulum stress. However, the mon1 deletion mutant showed more resistance to the antifungal agent fluconazole. In vivo studies demonstrated that compared to the wild-type strain, the mon1 deletion mutant attenuated virulence in the Galleria mellonella insect model. Moreover, the mon1 deletion mutant was avirulent in the murine inhalation model. These results demonstrate that Mon1 plays a crucial role in stress survival and pathogenicity in C. neoformans.

• Food Science and Biotechnology
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• 제17권4호
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• pp.829-832
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• 2008

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

• 천문학회보
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• 제46권2호
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• pp.50.1-50.1
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• 2021

The Gaia mission opens a new window to study the kinematics and dynamics of young stellar systems in detail. The kinematic properties of young stars provide vital constraints on the formation process of their host systems. Here, we present a kinematic study of the two associations Monoceros OB1 (Mon OB1) and R1 (Mon R1). Member candidates are first selected from the published list of member candidates, a compilation of OB star catalogues, and the classification of young stellar objects with the AllWISE data. According to the conventional wisdom, we selected a total of 728 members with similar proper motions at almost the same distance. Mon OB1 and Mon R1 have high levels of substructures that are also kinematically distinct. We identify six stellar groups in these associations, of which five show a pattern of expansion. In addition, the signature of rotation is found in two stellar groups of Mon OB1. Star formation history is inferred from a color-magnitude diagram. As a result, star formation in Mon OB1 has been sustained for several million years, while Mon R1 formed at almost the same epoch as the recent star formation in Mon OB1. Some old members in the outskirt of Mon OB1 have outward motions, which rules out the previously proposed outside-in star formation scenario. Star-forming regions including Mon OB1 and Mon R1 are found along a large arc-like gas structure. Hence, the formation of these two associations may originate from the hierarchical star formation along filaments in a turbulent molecular cloud.

• 한국산학기술학회논문지
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• 제16권4호
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• pp.2924-2932
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• 2015

본 연구는 초등학교, 중학교 및 고등학교의 급수설비 사용수량의 분석 통해 급수량 산출자료를 추정하여 학교시설 급수시스템의 적정 급수계획에 필요한 기초자료를 얻고자 하였다. 이를 위하여, 강원지역 6개 도시에 소재하는 초 중 고등학교 54개 학교를 대상으로 5년간의 급수설비 사용수량을 분석하였다, 분석결과, 1) 월평균 사용수량의 평균은 $600.7(m^3/mon)$이었으며, 초등학교와 중학교가 $484.2(m^3/mon)$, 고등학교가 $833.8(m^3/mon)$로 분석 되었다. 2) 학급당 월평균 사용수량의 평균은 $27.0(m^3/mon/class)$이었으며, 초등학교와 중학교가 $24.4(m^3/mon/class)$, 고등학교가 $32.(m^3/mon/class)$로 분석 되었다. 3) 1인1일 평균 사용수량의 평균은 55.8(l/c/d)이었으며, 초등학교와 중학교가 54.4(l/c/d), 고등학교가 58.6(l/c/d)로 분석 되었다.

• Mycobiology
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• 제47권4호
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• pp.457-465
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• 2019

MonA is a subunit of a guanine nucleotide exchange factor that is important for vacuole passing and autophagy processes in eukaryotes. In this study, we characterized the function of MonA, an orthologue of Saccharomyces cerevisiae Mon1, in the model fungus Aspergillus nidulans and a toxigenic fungus A. flavus. In A. nidulans, the absence of AnimonA led to decreased fungal growth, reduced asexual reproduction, and defective cleistothecia production. In addition, AnimonA deletion mutants exhibited decreased spore viability, had reduced trehalose contents in conidia, and were sensitive to thermal stress. In A. flavus, deletion of AflmonA caused decreased fungal growth and defective production of asexual spores and sclerotia structures. Moreover, the absence of monA affected vacuole morphology in both species. Taken together, these results indicate that MonA plays conserved roles in controlling fungal growth, development and vacuole morphology in A. nidulans and A. flavus.

• 한국축산식품학회지
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• 제34권5호
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• pp.683-691
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• 2014

The effects of sucrose stearate at various concentrations (0.1%, 0.2%, and 0.3%, w/v) on the physico-chemical characteristics of ready-to-eat (RTE) Samgyetang were investigated during storage at $25^{\circ}C$ for 12 mon. Over the storage duration, the addition of sucrose stearate had no significant effects on the proximate composition of Samgyetang, including meat, broth, and porridge, or the hardness and spreadability of the porridge, although it resulted in significantly higher CIE $L^*$ values for the porridge. The CIE $L^*$ values of Samgyetang porridge with added sucrose stearate increased until 9 mon, while the control decreased until 6 mon, and the values for both changed insignificantly thereafter. The breast meat of Samgyetang treated with sucrose stearate showed higher percentages of polyunsaturated fatty acid after 3 mon and lower percentages of monounsaturated fatty acid after 6 mon compared to the control (p<0.05), while no significant differences were observed with the different sucrose stearate concentrations (p>0.05). The overall sensory acceptability scores were higher at sucrose stearate concentrations of 0.2% or 0.3% after 6 mon and at 0.1% after 9 mon compared to those of the control.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1425-1434
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• 2008

The study was conducted to evaluate and compare the effects of feed additives on ruminal fermentation, nutrient digestibility and performance of lambs fed diets containing 60% wet brewers grains (WBG). In Experiment 1, two simultaneous trials were conducted. Fifty intact ($20.2{\pm}0.8kg\;BW$) lambs were used in a feedlot trial and 10 (rumen cannulated; $32{\pm}1kg\;BW$) in a digestion trial. The pH, volatile fatty acids (VFA) and ammonia-N in lambs were also estimated. Lambs were randomly assigned to one of five diets: i) without additives (Con), ii) with 1% bicarbonate (Bic), iii) with 1% bentonite (Ben), iv) with 33 mg/kg monensin (Mon) and v) with 200 mg/kg fibrolityc enzymes (Enz). In Experiment 2, 120 RambouilletPelibuey intact male lambs ($19.5{\pm}1.5kg\;BW$) were used in a feedlot trial and randomly assigned to four diets: i) without additives (control), ii) with 1% Bic, iii) with 33 mg/kg Mon and iv) with 1% Bic and 33 mg/kg Mon. In Experiment 1, lambs fed diets containing Bic or Mon had significantly higher final weight, DMI, ADG than other lambs. However, apparent DM, OM, CP, NDF and ADF digestibilities and ruminal individual VFA content were similar (p>0.05) among treatments. Conversely, treatmentcollection period interaction was significant for ruminal pH and NH3. In Experiment 2, lambs fed diets containing a Bic and Mon combination had significantly higher final weight, DMI and ADG. It is concluded lambs fed Bic or Mon or Bic and Mon combination had better performance characteristics than lambs on Ben or Enz.

• 한국균학회지
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• 제38권1호
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• pp.25-28
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• 2010

Di-mon 교잡에 의해 만들어진 표고품종 산림7호를 원목 재배한 결과, 1년 중 가을발생이 가장 많았으며, 생표고용으로 적합하였다. 발생된 버섯은 평반구형이며, 갓의 직경은 47mm 정도 되었다. 주름살 측면은 평활형이며, 폭은 좁았다. 버섯발 이온도는 평균 $11{\sim}23^{\circ}C$였고, 산발발생하는 경향이 있었다. 그리고 4년간 생산된 버섯량은 골목 1본당 약 1.4 kg이었다.

• 정보과학회 논문지
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• 제42권3호
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• pp.295-306
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• 2015

MOnCa2는 스마트폰에 장착된 센서와 온톨로지 추론 기반의 지능형 스마트폰 어플리케이션 구축을 위한 프레임워크다. 기존에 연구되었던 MOnCa는 온톨로지 인스턴스로 등록된 센서 값에 대한 정보를 바탕으로 사용자의 현재 상황을 판단 및 추론하였다. 이러한 방식은 사용자의 공간 정보나 주변에 존재하는 객체가 무엇인지 판단하는 것은 가능하나 사용자의 물리적인 콘텍스트(이동 행위, 이동할 목적지 등등) 판단하는 것은 불가능했다. 본 논문에서 설명하는 MOnCa2는 사용자 개개인의 물리적인 콘텍스트를 판단 및 추론하기 위해 스마트폰의 장착된 센서를 바탕으로 행위 및 이동 상황에 대응하는 인지 모델을 구축하고, 구축된 모델을 기반으로 사용자의 실시간 행위 및 이동 상황에 대해 1차적인 추론을 수행하며, 추론된 1차적인 콘텍스트에 대해 온톨로지 기반의 2차 추론을 통해 지능형 어플리케이션에 필요한 고수준 사용자 콘텍스트를 생산한다. 따라서 본 논문은 스마트폰의 가속도 센서를 기반으로 사용자의 이동에 필요한 행위를 인지하는 기법, 스마트폰의 GPS 신호를 바탕으로 이동 목적지와 경로를 예측하는 기법, 온톨로지 실체화를 적용하여 고수준 콘텍스트를 추론하는 과정에 초점을 맞추어 설명을 한다.

• 한국축산식품학회지
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• 제32권4호
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• pp.414-427
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• 2012

Carcass characteristics and meat quality traits as a function of endpoint months of slaughter age (26 vs 32 mon) and chiller ageing (1 vs 10 d) were evaluated for m. longissmus of 26 Hanwoo steers fed with commercial diets including whole crop barley silage. Totally twenty six Hanwoo steers for 6 mon of age that were fed until 26 mon of age constituted the short term-fed group and fed until 32 mon of age constituted long-term fed group. Carcasses were chilled for 24 h and were graded. Strip loin samples were divided into two age groups (1 d and 10 d). Long-term feeding increased carcass weight, rib-eye area, yield grade, marbling score, firmness and quality grade of the meat. The feeding for 32 mon produced tender, juicy meat (p<0.01) with lower cooking loss and higher rating score (p<0.05) than short term feeding, while other quality traits were not influenced by the length of feeding. Intramuscular fat content and oxidative stability (TBARS value) were significantly (p<0.05) higher in beef from long-term feeding however the length of feeding did not alter the fatty acid composition. Chiller aging reduced instrumental tenderness (WBSF value), improved color, sensory tenderness, acceptability and rating of beef. The results of the present study mirrors that Hanwoo steers until 32 mon of age overall improved carcass traits and palatability compared to that for 26 mon. However, from the viewpoints of economical and environmental aspects, cost of the additional feeding for 6 mon for value-adding of eating quality was relatively high and the effects in turn were limited.