• Title/Summary/Keyword: Molecular test

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The Rheological Behaviours for Ink Vehicle According to Molecular Weight of Rosin Modified Phenolic Resin (변성 페놀 수지의 분자량 변화에 따른 잉크 비히클의 물성 변화에 관한 연구)

  • Kim, Tae-Hwan;Kim, Sung-Bin;Lee, Kyu-Il
    • Journal of the Korean Graphic Arts Communication Society
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    • v.23 no.2
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    • pp.1-14
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    • 2005
  • Generally, printing inks are composed of pigment, vehicle and additive. Among others, the vehicle transfers the pigment to substrate and then binds it on the surface. So, rheological properties of the vehicle are an important factor which has influence on printability. Thus, in this study, rheology of the vehicle was investigated by using rotational rheometer according to molecular weight of resin. Also, emlusion rheology of water in oil type and its microstructure were examined with increasing the shear rate. Consequently, the following results were obtained: (1) By viscometric flow test, zero shear viscosity and shear thinning index of vehicle increased with increasing the molecular weight of resin. (2) By relaxation and creep test, relaxation time and retardation time of vehicle increased with increasing the molecular weight of resin. (3) By frequency sweep test, crossover point of vehicle increased with increasing the molecular weight of resin. (4) G' and G" of emlusions increased with increasing the molecular weight by amplitude sweep test. (5) The shape of water drop in emlusions was changed to the capillary tube.

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What Determines Star Formation Rates?

  • Evans, Neal
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.2
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    • pp.29.4-29.4
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    • 2016
  • The relations between star formation and properties of molecular clouds are studied based on a sample of star forming regions in the Galactic Plane. Sources were selected by having radio recombination lines to provide identification of associated molecular clouds and dense clumps. Radio continuum and mid-infrared emission were used to determine star formation rates, while 13CO and submillimeter dust continuum emission were used to obtain masses of molecular and dense gas, respectively. We test whether total molecular gas or dense gas provides the best predictor of star formation rate. We also test two specific theoretical models, one relying on the molecular mass divided by the free-fall time, the other using the free-fall time divided by the crossing time. Neither is supported by the data. The data are also compared to those from nearby star forming regions and extragalactic data. The star formation "efficiency," defined as star formation rate divided by mass, spreads over a large range when the mass refers to molecular gas; the standard deviation of the log of the efficiency decreases by a factor of three when the mass of relatively dense molecular gas is used rather than the mass of all the molecular gas.

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Combined Cytogenetic and Molecular Analyses for the Diagnosis of Prader-Willi/Angelman Syndromes

  • Borelina, Daniel;Engel, Nora;Esperante, Sebastian;Ferreiro, Veronica;Ferrer, Marcela;Torrado, Maria;Goldschmidt, Ernesto;Francipane, Liliana;Szijan, Irene
    • BMB Reports
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    • v.37 no.5
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    • pp.522-526
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    • 2004
  • Prader-Willi (PWS) and Angelman (AS) are syndromes of developmental impairment that result from the loss of expression of imprinted genes in the paternal (PWS) or maternal (AS) 15q11-q13 chromosome. Diagnosis on a clinical basis is difficult in newborns and young infants; thus, a suitable molecular test capable of revealing chromosomal abnormalities is required. We used a variety of cytogenetic and molecular approaches, such as, chromosome G banding, fluorescent in situ hybridization, a DNA methylation test, and a set of chromosome 15 DNA polymorphisms to characterize a cohort of 27 PWS patients and 24 suspected AS patients. Molecular analysis enabled the reliable diagnosis of 14 PWS and 7 AS patients, and their classification into four groups: (A) 6 of these 14 PWS subjects (44%) had deletions of paternal 15q11-q13; (B) 4 of the 7 AS patients had deletions of maternal 15q11-q13; (C) one PWS patient (8%) had a maternal uniparental disomy (UPD) of chromosome 15; (D) the remaining reliably diagnoses of 7 PWS and 3 AS cases showed abnormal methylation patterns of 15q11-q13 chromosome, but none of the alterations shown by the above groups, although they may have harbored deletions undetected by the markers used. This study highlights the importance of using a combination of cytogenetic and molecular tests for a reliable diagnosis of PWS or AS, and for the identification of genetic alterations.

SECOND-PHASE VALIDATION STUDY OF AN ALTERNATIVE DEVELOPMENTAL TOXICITY TEST USING MOUSE EMBRYONIC STEM CELL-DERIVED EMBRYOID BODIES

  • J.-H. LEE;S.Y. PARK;C. AHN;Y.-M. YOO;C.-W. KIM;J.-E. KIM;N.R. JO;H.Y. KANG;E.-M. JUNG;K.-S. KIM;K.-C. CHOI;S.D. LEE;E.-B. JEUNG
    • The Korean Journal of Physiology and Pharmacology
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    • v.71 no.2
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    • pp.223-233
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    • 2020
  • The embryoid body test (EBT) is a developmental toxicity test method that measures the size of embryoid bodies (EBs) and the viability of mouse embryonic stem cells (mESCs) and fibroblasts (3T3 cells). The previous pre-validation study confirmed the high accuracy (above 80%) of EBT using 26 coded test chemicals. This second-phase validation study assessed the inter-laboratory reproducibility (5 chemicals in common) and predictive capacity (10 chemicals in each laboratory) test using the coded test chemicals at three laboratories. For the prediction model, the accuracy is increased when more data is accumulated. Therefore, we updated the prediction model and analyzed the results of the second year with the newly created-prediction model. Statistical analysis of the inter-laboratory reproducibility test results indicated that accuracy, sensitivity, and specificity were 87%, 78%, and 100%, respectively. The results of the statistical analysis of the predictive capacity test showed an accuracy of 80%, sensitivity of 78%, and specificity of 81%. In conclusion, the EBT can accurately classify various embryotoxicants within a short period and with relatively little effort. Therefore, EBT can be used as a good way to test developmental toxicity.

Molecular Dynamics Simulation for Compression Test of PMMA Nano Pillars (PMMA 나노 기둥의 압축시험에 대한 분자동역학 해석)

  • Kim, Jung-Yup;Kim, Jae-Hyun;Choi, Byung-Ik
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.502-505
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    • 2007
  • PMMA has been extensively adopted in Nano Imprint Lithography(NIL). PMMA nano-structures experience severe mechanical load and deformation during NIL process, and understanding its mechanical behavior is very important in designing and optimizing NIL process. One of the most promising techniques for characterizing the mechanical behavior of nano structures is nano pillar compression test. In this study, the mechanical behaviors of PMMA pillars during compression test are analyzed using Molecular Dynamics. Two methods for simulation of PMMA nano pillars are proposed. The stress-strain relationship of nano-scale PMMA structure is obtained based on CVFF(Covalent Valence Force Fields) potential and the dependency of the applied strain rate on the stress-strain relationship is analyzed. The obtained stress-strain relationships can be useful in simulating nano-scale PMMA structures using Finite Element Method(FEM) and understanding the experimental results obtained by compression test of PMMA nano pillars.

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Development of Turbo Molecular Pump Vacuum Facility for High Altitude Space Environment Test (고고도 우주환경모사용 터보분자펌프 진공설비 구축)

  • Huh, Hwan-Il;Kim, Min-Jae;Kim, Sung-Su
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.827-829
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    • 2011
  • Vacuum facility is required for high altitude space environment test to develop satellites or space launch vehicles. We, at Chungnam, National University, developed turbo molecular pump vacuum test facility up to $1.0{\times}10-6$ torr to simulate 200 km altitude environment. In this paper, we present some preliminary vacuum performance test results.

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Inhibition of Overexpressed CDC-25.1 Phosphatase Activity by Flavone in Caenorhabditis elegans

  • Kim, Koo-Seul;Kawasaki, Ichiro;Chong, Youhoon;Shim, Yhong-Hee
    • Molecules and Cells
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    • v.27 no.3
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    • pp.345-350
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    • 2009
  • We previously reported that flavone induces embryonic lethality in Caenorhabditis elegans, which appeared to be the result of cell cycle arrest during early embryogenesis. To test this possibility, here we examined whether flavone inhibits the activity of a key cell cycle regulator, CDC-25.1 in C. elegans. A gain-of-function cdc-25.1 mutant, rr31, which exhibits extra cell divisions in intestinal cells, was used to test the inhibitory effects of flavone on CDC-25 activity. Flavone inhibited the extra cell divisions of intestinal cells in rr31, and modifications of flavone reduced the inhibitory effects. The inhibitory effects of flavone on CDC-25.1 were partly, if not completely, due to transcriptional repression.

Distribution of Pathogenic Genes and Molecular Typing of Yersinia pseudotuberculosis isolated from Spring Water in Seoul

  • Kim, Mi-Sun;Shim, Mi-Ja
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.161.2-161.2
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    • 2003
  • In order to investigate the pathogenic genes and genetic relationships of Y. pseudotuberculosis, we isolated 9 strains of Y. pseudotuberculosis from about 380 spring water sites in Seoul and carried out antibiotic susceptibility test, biological test and molecular typing. All isolated strains were distributed throughout the northeast area in Seoul (Mt. Bookhan, Mt. Soorak, Mt. Boolam and etc...).Antibiotic susceptibility test revealed that all the strains were susceptible to chloramphenicol, gentamicin, neomycin and amoxicillin/clavulanic acid, but were resistant to novobiocin and vancomycin. (omitted)

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Consideration on the performance test procedure of a turbomolecular pump and a compound molecular pump (터보분자펌프 및 복합분자펌프의 성능시험절차에 대한 고찰)

  • 인상렬;박미영
    • Journal of the Korean Vacuum Society
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    • v.10 no.3
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    • pp.303-311
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    • 2001
  • The pumping speed and the maximum compression ratio as well as the critical backing pressure as main parameters representing normal and limiting performance of a turbomolecular pump and a compound molecular pump were measured basing on a draft KS of the acceptance test procedure. Characteristics of the molecular pumps of two different kinds were compared and some points for improving regulations of the test procedure were proposed.

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