Browse > Article
http://dx.doi.org/10.1007/s10059-009-0044-9

Inhibition of Overexpressed CDC-25.1 Phosphatase Activity by Flavone in Caenorhabditis elegans  

Kim, Koo-Seul (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Kawasaki, Ichiro (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Chong, Youhoon (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Shim, Yhong-Hee (Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University)
Publication Information
Molecules and Cells / v.27, no.3, 2009 , pp. 345-350 More about this Journal
Abstract
We previously reported that flavone induces embryonic lethality in Caenorhabditis elegans, which appeared to be the result of cell cycle arrest during early embryogenesis. To test this possibility, here we examined whether flavone inhibits the activity of a key cell cycle regulator, CDC-25.1 in C. elegans. A gain-of-function cdc-25.1 mutant, rr31, which exhibits extra cell divisions in intestinal cells, was used to test the inhibitory effects of flavone on CDC-25 activity. Flavone inhibited the extra cell divisions of intestinal cells in rr31, and modifications of flavone reduced the inhibitory effects. The inhibitory effects of flavone on CDC-25.1 were partly, if not completely, due to transcriptional repression.
Keywords
Caenorhabditis elegans; CDC-25 phosphatase inhibitor; flavone (2-phenyl chromone); hyperplasia; intestinal cell division;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
연도 인용수 순위
1 Ashcroft, N.R., Kosinski, M.E., Wickramasinghe, D., Donovan, P.J., and Golden, A. (1998). The four cdc25 genes from the nematode Caenorhabditis elegans. Gene 214, 59-66   DOI   PUBMED   ScienceOn
2 Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94   PUBMED
3 Clucas, C., Cabello, J., Bussing, I., Schnabel, R., and Johnstone, I.L. (2002). Oncogenic potential of a C. elegans cdc25 gene is demonstrated by a gain-of-function allele. EMBO J. 21, 665-674   DOI   ScienceOn
4 Draetta, G., and Eckstein, J. (1997). Cdc25 protein phosphatases in cell proliferation. Biochim. Biophys. Acta. 1332, M53-63   PUBMED
5 Ngan, E.S., Hashimoto, Y., Ma, Z.Q., Tsai, M.J., and Tsai, S.Y. (2003). Overexpression of Cdc25B, an androgen receptor coactivator, in prostate cancer. Oncogene 22, 734-739   DOI   PUBMED   ScienceOn
6 Sadhu, K., Reed, S.I., Richardson, H., and Russell, P. (1990). Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2. Proc. Natl. Acad. Sci. USA 87, 5139-5143   DOI   ScienceOn
7 Imada, K., Lin, N., Liu, C., Lu, A., Chen, W., Yano, M., Sato, T., and Ito, A. (2008). Nobiletin, a citrus polymethoxy flavonoid, suppresses gene expression and production of aggrecanases-1 and -2 in collagen-induced arthritic mice. Biochem. Biophys. Res. Commun. 373, 181-185   DOI   ScienceOn
8 Kim, S., and Shim, J. (2008). A forward genetic approach for analyzing the mechanism of resistance to the anti-cancer drug, 5- fluorouracil, using Caenorhabditis elegans. Mol. Cells 25, 119-123   PUBMED
9 Brezak, M.C., Quaranta, M., Mondesert, O., Galcera, M.O., Lavergne, O., Alby, F., Cazales, M., Baldin, V., Thurieau, C., Harnett, J., et al. (2004). A novel synthetic inhibitor of CDC25 phosphatases: BN82002. Cancer Res. 64 3320-3325   DOI   ScienceOn
10 Galaktionov, K., Lee, A.K., Eckstein, J., Draetta, G., Meckler, J., Loda, M., and Beach, D. (1995). CDC25 phosphatases as potential human oncogenes. Science 269, 1575-1577   DOI   PUBMED
11 Hebeisen, M., and Roy, R. (2008). CDC-25.1 stability is regulated by distinct domains to restrict cell division during embryogenesis in C. elegans. Development 135, 1259-1269   DOI   ScienceOn
12 Lee, Y.U., Kawasaki, I., Lim, Y., Oh, W.S., Paik, Y.K., and Shim, Y.H. (2008). Inhibition of developmental processes by flavone in Caenorhabditis elegans and its application to the pinewood nematode, Bursaphelenchus xylophilus. Mol. Cells 26, 171-174   PUBMED
13 Ullmannova, V., and Popescu, N.C. (2007). Inhibition of cell proliferation, induction of apoptosis, reactivation of DLC1, and modulation of other gene expression by dietary flavone in breast cancer cell lines. Cancer Detect. Prev. 31, 110-118   DOI   ScienceOn
14 Xing, X., Chen, J., and Chen, M. (2008). Expression of CDC25 phosphatases in human gastric cancer. Dig. Dis. Sci. 53, 949-953   DOI   ScienceOn
15 Zhang, Q., Zhao, X.H., and Wang, Z.J. (2008). Flavones and flavonols exert cytotoxic effects on a human oesophageal adenocarcinoma cell line (OE33) by causing G2/M arrest and inducing apoptosis. Food Chem. Toxicol. 46, 2042-2053   DOI   ScienceOn
16 Kostic, I., and Roy, R. (2002). Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegans. Development 129, 2155-2165   PUBMED
17 Ashcroft, N.R., Srayko, M., Kosinski, M.E., Mains, P.E., and Golden, A. (1999). RNA-Mediated interference of a cdc25 homolog in Caenorhabditis elegans results in defects in the embryonic cortical membrane, meiosis, and mitosis. Dev. Biol. 206, 15-32   DOI   ScienceOn
18 Hofmann, K., Bucher, P., and Kajava, A.V. (1998). A model of Cdc25 phosphatase catalytic domain and Cdk-interaction surface based on the presence of a rhodanese homology domain. J. Mol. Biol. 282, 195-208   DOI   ScienceOn
19 Ono, M., Maya, Y., Haratake, M., and Nakayama, M. (2007). Synthesis and characterization of styrylchromone derivatives as beta-amyloid imaging agents. Bioorg. Med. Chem. 15, 444-450   DOI   ScienceOn
20 Contour-Galcera, M.O., Lavergne, O., Brezak, M.C., Ducommun, B., and Prevost, G. (2004). Synthesis of small molecule CDC25 phosphatases inhibitors. Bioorg. Med. Chem. Lett. 14, 5809-5812   DOI   ScienceOn
21 Boutros, R., Lobjois, V., and Ducommun, B. (2007). CDC25 phosphatases in cancer cells: key players? Good targets? Nat. Rev. Cancer 7, 495-507   DOI   ScienceOn
22 Xu, X., Yamamoto, H., Sakon, M., Yasui, M., Ngan, C.Y., Fukunaga, H., Morita, T., Ogawa, M., Nagano, H., Nakamori, S., et al. (2003). Overexpression of CDC25A phosphatase is associated with hypergrowth activity and poor prognosis of human hepatocellular carcinomas. Clin. Cancer Res. 9, 1764-1772