• 한국산림과학회지
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• 제101권2호
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• pp.291-296
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• 2012

세대기간이 긴 임목에 있어 3배체는 바이오매스 생산과 분자생물학적 연구 분야에서 매우 유용한 연구재료이다. 1970년대 육성된 현사시나무 4배체와 일반 2배체 간의 인공교배를 통해 3배체가 육성되었다. 유세포 분석법을 이용해 이들 $F_1$의 배수성을 확인한 결과 14개체 중 10개체가 3배체로 선발되었다. 그 가운데 3계통의 3배체(Line-1, Line-17, Line-18)는 기내배양시 2배체에 비해 잎이 크고, 엽형이 다소 변형된 형태적 특징이 있었다. 특히 'Line-18'은 줄기가 2배체에 비해 굵었으며, 뿌리 생육에 있어서 발근이 더뎠고 뿌리수도 다른 3배체나 2배체에 비해 적은 특징을 보였다. 그러나 순화율은 모두 100%에 달하였다. 본 연구결과는 3배체 현사시나무가 바이오매스 생산 및 형질전환 연구를 위한 좋은 재료가 될 수 있음을 보여주는 결과이며, 또한 유세포 분석법이 배수체 육종시 배수체를 확인하는데 효율적인 방법임을 시사한다.

• Journal of Plant Biotechnology
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• 제34권1호
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• pp.7-10
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• 2007

어리연꽃의 화경조직으로부터 형성된 캘러스로부터 체세포배발생을 통한 기내 식물체 재생체계를 확립하였다. 2,4-D가 첨가된 배양배지에 4주 배양 후 어리연꽃의 화경화경조직으로부터 연황색의 구형세포과가 발달하였으며 생장조절제가 첨가되지 않은 1/2MS 배지로 옮걱 명배양한 결과, 약 배양2주 후부터 녹색의 체세포배 유사구조의 발달이 관찰되었으며 정상적인 식물체로 발달하였다. 따라서 캘러스 발달 초기에 형성된 구형 세포괴 및 캘러스는 초기 단계의 체세포배이며 캘러스는 배발생캘러스임을 알 수 있었다. 화경조직으로부터 배발생캘러스 형성빈도는 0.1-0.3 mg/L 2,4-D가 첨가된 1/2MS 배지에서는 거의 100%이었으며 2,4-D 농도가 증가할수록 배발생캘러스의 형성빈도는 반대로 감소하였고 고농도 2,4-D 처리구에서는 캘러스 형성이 전혀 이루어지지 않았다. 한편 BA는 처리 농도에 상관없이 조직의 갈변 및 고사를 유발함으로써 체세포배 형성에 저해적임을 알 수 있었다. 어리연꽃의 잎조직의 경우는 화경조직에 비하여 연황색 구형 세포괴 형성 빈도가 매우 낮아 0.3 mg/L 2,4-D 처리구 (배발생캘러스 형성빈도 9.5%)를 제외하고는 나머지 모든 처리구에서 배발생캘러스의 형성을 관찰할 수 없었다. 현재 배발생캘러스의 현탁배양 체계를 확립중이며 본 연구에서 확립된 어리연꽃의 식물체 재생체계는 어리연꽃의 기내 대량증식 수단은 물론, 유용 형질 도입을 통한 분자육종의 수단으로 활용이 가능할 것으로 기대된다.

• 한국패류학회지
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• 제28권4호
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• pp.377-384
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• 2012

The importance of biological resources has been gradually increasing, and mollusks have been utilized as main fishery resources in terrestrial ecosystems. But little is known about genomic and transcriptional analysis in mollusks. This is the first report on the transcriptomic profile of Meretrix lusoria. In this study, we constructed cDNA library and determined 542 of distinct EST sequences composed of 284 singletons and 95 contigs. At first, we identified 180 of EST sequences that have significant hits on protein sequences of the exclusive Mollusks database through BLASTX program and 343 of EST sequences that have significant hits on NCBI NR database. We also found that 211 of putative sequences through local BLAST (blastx, E < e-10) search against KOG database were classified into 16 functional categories. Some kinds of immune response related genes encoding allograft inflammatory factor 1 (AIF-1), B-cell translocation gene 1 (BTG1), C-type lectin A, thioester-containing protein and 26S proteasome regulatory complex were identified. To determine phylogenetic relationship, we identified partial sequences of four genes (COX1, COX2, 12S rRNA and NADH dehydrogenase) that significantly matched with the mitochondrial genomes of 3 species-Ml (Meretrix lusoria), Mp (Meretrix petechialis) and Mm (Meretrix meretrix). As a result, we found that there was a little bit of a difference between sequences of Korean isolates and other known isolates. This study will be useful to develop breeding technology and might also be helpful to establish a classification system.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.227-227
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• 2017

This study was conducted to analyze the reduction of quality and ripening rate of rice due to submergence during ripening stage and identify the physiological cause. Korean japonica type rice cultivars, Nampyeong was used in the experiment. The following 7, 14 day after heading, they were then moved into submergence treatment facility and we conducted the tests under three different submergence conditions - T1(clear water and overhead submergence), T2(Muddy water and exposure of Flag leaf end ), T3(Muddy water and overhead submergence), and the treatment lasted for 4days. The decrease of ripening rate was most severe in T3 treatment at 7days after heading, and the decrease of head rice ratio was most severe in T3 treatment at 14days after heading. Meanwhile the starch synthesis was inhibited, as the supply of assimilation products was inhibited in grain during the submergence treatment. And in stem, sucrose content was increased because the soluble carbohydrates accumulated before heading were decomposed. These changes may be due to the consumption of respiratory substrate in anaerobic conditions and the inhibition of the production of photosynthetic products by light interception. In order to see what kind of reaction occurs at the molecular level, we examined the degree of RNA expression in grain, stem and leaf. First, the expression of rna associated with starch synthesis in grain was decreased in all submergence treatment. and especially Ospul(DBE) was more decreased in 14days after heading treatment than 7days after heading treatment. This difference can explain the result that the decrease of head rice ratio was more severe at 14days after heading. And in stem, the expression of rna associated with the supply of assimilation products was decreased in submergence treatment. Finally in leaf, the expression of rna(ADH, ALDH) associated with anaerobic respiration was increased, while the expression of rna associated with photosynthesis was decreased. These results of physiological analysis can used to develop the cultivation technique and to offer the information for breeding the cultivars with tolerant characteristics to submergence stress during ripening stage in rice.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.127-127
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• 2017

Aluminum is the most abundant metallic element in the Earth's crust and considered as the most limiting factor for plant productivity in acidic soils. The inhibition of root growth is recognized as the primary effect of Al toxicity. Seeds of wheat cv. Keumkang (Korean cultivar) were germinated on petridish for 5 days and then transferred hydroponic apparatus which was treated with $0{\mu}M$ $AlCl_3$ (control), $100{\mu}M$ $AlCl_3$ and $150{\mu}M$ $AlCl_3$ for 5 days. The length of roots, shoots and fresh weight of wheat seedlings were decreased under aluminum stress. The concentrations of $K^+$, $Mg^{2+}$ and $Ac^{2+}$ were decreased whereas $Al^{3+}$ and $P_2O_5{^-}$ concentration was increased under aluminum stress. Using confocal microscopy, the fluorescence intensity of aluminum was increased with morin staining. In this study, a proteome analysis was performed to identify proteins, which is responsible to aluminum stress in wheat roots. In 10-day-old seedlings, proteins were extracted from roots and separated by 2-DE, stained by CBB. Using image analysis, a total of 47 differentially expressed protein spots were selected, whereas 19 protein spots were significantly up-regulated such as s-adenosylmethionine, oxalate oxidase, malate dehydrogenase, cysteine synthase, ascorbate peroxidase and 28 protein spots were significantly down-regulated such as heat shock protein 70, o-methytransferase 4, enolase, amylogenin by aluminum stress following protein spots analyzed by LTQ-FTICR mass spectrometry. The results provide the global picture of Al toxicity-induced alterations of protein profiles in wheat roots, and identify the Al toxicity-responsive proteins related to various biological processes that may provide some novel clues about plant Al tolerance.

• The Plant Pathology Journal
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• 제23권3호
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• pp.143-150
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• 2007

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

• Journal of Plant Biotechnology
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• 제30권3호
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• pp.281-291
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• 2003

In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.

• 한국임상수의학회지
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• 제32권1호
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• pp.1-4
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• 2015

개의 10번 염색체에 존재하는 Copper metabolism domain containing 1 (COMMD1) 유전자는 체내 구리 대사를 조절하는 COMMD1 단백질을 합성한다. COMMD1 유전자의 exon 2 결손변이는 단백질의 결핍을 유발하여 베들링턴 테리어 견종에서 상염색체 열성 유전질환인 구리 중독증을 일으킨다. 본 증에 이환된 개체는 담즙을 통한 구리의 배설이 저해되어 간 내에 구리가 축적된다. 본 연구에서는 국내 베들링턴 테리어 257두(수컷 109두, 암컷 148두) 혈액 시료를 사용하여 genomic DNA를 추출하였다. 유전자 결손변이의 분자생물학적 진단을 위해 다중 중합효소 연쇄반응법(multiplex PCR)을 이용하여 COMMD1 유전자의 exon 2 결손 발생 및 그 빈도를 조사하였다. 베들링턴 테리어 257두에서, 정상유전자 동협접합자가 131두(51%), 이형접합자가 108두(42%), 변이유전자 동형접합자가 18두(7%)로 확인되었다. 본 연구를 통해 한국 베들링턴 테리어 개체군의 유전변이 발생 및 그 빈도를 확인하였고, 이는 국내 베들링턴 테리어 개체군의 유전자 선택적 교배계획 설립 및 변이 유전자 확산을 예방하기 위한 기초 자료로서 의의가 있다.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.177-186
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• 2005

Xenotransplantation may help to overcome the critical shortage of human tissues and organs for human transplantation, Swine represents an ideal source of such organs owing to their anatomical and physiological similarities to human besides their plentiful supply, However, the use of organs across the species barrier may be associated with the risk of transmission of pathogens, specially porcine endogenous retroviruses (PERVs).• Although most of these potential pathogens could be eliminated by pathogen-free breeding, PERVs are not eliminated by this treatment. PERVs are integrated into the genome of all pigs and produced by normal pig cells and infect human cells. They belong to gamma retroviruses and are of three classes viruses: A, B and C. In the present study, PCR based cloning was performed with chromosomal DNA extracted from pigs from domestic pigs in Korea. Amplified PCR fragments of about 1.5 Kb, covering the partial env gene, were cloned into pCR2.l-TOPO vectors and sequenced. A total of 91 env clones were obtained from domestic pigs, Berkshire, Duroc, Landrace and Yorkshire in Korea. Phylogenetic analysis of these genes revealed the presence of only PERV class A and B in the proportion of 58 % and 42 %, respectively. Among these, 28 clones had the correct open reading frame: 18 clones in class A and 10 clones in class B. Since both these PERV classes are polytropic and have the capacity to infect human cells, our data suggest that proviral PERVs have the potential to generate infectious viruses during or after xenotransplantation in human.

• Journal of Plant Biotechnology
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• 제30권1호
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• pp.19-25
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• 2003

본 실험은 방사선 처리에서 유기된 벼 변이계통의 몇 가지 농경 형질 분석 및 RAPD 분석을 통하여 방사선 이용 돌연변이 육종연구를 위한 기초적인 자료를 얻기 위하여 실시하였다. 1. 방사선에 의해 선발된 변이 계통의 초장, 수장 및 내도복성 형질은 모품종과 비교하여 정 (＋)및 부 (－)의 방향으로 작용하였다. 2. RAPD 분석에 의해 계통 간 polymorphic band를 관찰할 수 있었고, UPGMA에 의해 변이계통를 4 groups으로 분류할 수 있었다. 3. 변이체들 중 계통 91, 139, 140 및 141은 내염성 형질을 나타냈고, 유리 proline 함량이 모품종보다 유의성 있는 증가를 나타내었다. 4. 139, 140 및 141 계통은 dendrogram에서 같은 그룹을 이루었고 모품종과 가장 먼 유전적 거리를 나타내었다. 5. 이러한 계통들은 육종 및 분자유전 연구에 유용한 재료로 이용될 수 있다.