Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Cloning and Phylogenetic Analysis of PERVs from Domestic Pigs in Korea (env gene sequences)

국내 돼지에 존재하는 내인성 레트로 바이러스의 엔밸로프 유전자 클로닝 및 분자 계통학적 분석

  • Lee, Dong-Hee (Department of Biotechnology, College of Animal Husbandry, Konkuk University, Department of Genetic Engineering, Faculty of Lifescience and technology, Sungkyunkwan University) ;
  • Yoo, Jae-Young (Department of Biotechnology, College of Animal Husbandry, Konkuk University) ;
  • Lee, Jung-Eun (Department of Biotechnology, College of Animal Husbandry, Konkuk University) ;
  • Kim, Gye-Woong (Department of Animal resource science, College of Industrial Science, Kongju National University) ;
  • Park, Hong-Yang (Department of Biotechnology, College of Animal Husbandry, Konkuk University) ;
  • Lee, Hoon-Taek (Department of Biotechnology, College of Animal Husbandry, Konkuk University) ;
  • Kim, Young-Bong (Department of Biotechnology, College of Animal Husbandry, Konkuk University)
  • 이동희 (건국대학교 축산대학 동물생명공학 전공) ;
  • 유재영 (건국대학교 축산대학 동물생명공학 전공) ;
  • 이정은 (건국대학교 축산대학 동물생명공학 전공) ;
  • 김계웅 (공주대학교 산업과학대학 동물자원학과) ;
  • 박홍양 (건국대학교 축산대학 동물생명공학 전공) ;
  • 이훈택 (건국대학교 축산대학 동물생명공학 전공) ;
  • 김영봉 (건국대학교 축산대학 동물생명공학 전공)
  • Published : 2005.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Xenotransplantation may help to overcome the critical shortage of human tissues and organs for human transplantation, Swine represents an ideal source of such organs owing to their anatomical and physiological similarities to human besides their plentiful supply, However, the use of organs across the species barrier may be associated with the risk of transmission of pathogens, specially porcine endogenous retroviruses (PERVs).• Although most of these potential pathogens could be eliminated by pathogen-free breeding, PERVs are not eliminated by this treatment. PERVs are integrated into the genome of all pigs and produced by normal pig cells and infect human cells. They belong to gamma retroviruses and are of three classes viruses: A, B and C. In the present study, PCR based cloning was performed with chromosomal DNA extracted from pigs from domestic pigs in Korea. Amplified PCR fragments of about 1.5 Kb, covering the partial env gene, were cloned into pCR2.l-TOPO vectors and sequenced. A total of 91 env clones were obtained from domestic pigs, Berkshire, Duroc, Landrace and Yorkshire in Korea. Phylogenetic analysis of these genes revealed the presence of only PERV class A and B in the proportion of 58 % and 42 %, respectively. Among these, 28 clones had the correct open reading frame: 18 clones in class A and 10 clones in class B. Since both these PERV classes are polytropic and have the capacity to infect human cells, our data suggest that proviral PERVs have the potential to generate infectious viruses during or after xenotransplantation in human.

Keywords

References

  1. Akiyoshi, D. E., Denaro, M., Zhu H., Greenstein, J. L., Banerjee, P. and Fishman, J. A. 1998. Identification of a full-length cDNA for an endogenous retrovirus of miniature swine. J Virol. 72(5): 4503-4507
  2. Breese, S. S. Jr. 1970. Virus-like particles occurring in cultures of stable pig kidney cell lines. Brief report. Arch Gesamte Virusforsch 30:401-404 https://doi.org/10.1007/BF01258369
  3. Cianciolo, G. J., Kipnis, R. J. and Synderman, R. 1984. Similarity between p15E of murine and feline leukemia viruses and p21 of HTLV. Nature 311515
  4. Dorling, A. 2002. Clinical xenotransplantation; pigs might fly? Am J Transplant. 2:695-700 https://doi.org/10.1034/j.1600-6143.2002.20803.x
  5. Harrison, I., Takeuchi, Y., Bartosch, B. and Stoye, J. P. 2004. Determinants of high titer in recombinant porcine endogenous retroviruses. J Virol. 78(24):13871-13879 https://doi.org/10.1128/JVI.78.24.13871-13879.2004
  6. Heneine, W., Tibell, A., Switzer, W. M., Sandstrom, P., Rosales, G. V., Mathews, A., Korsgren, O., Chapman, L. E., Folks, T. M. and Groth, C. G. 1998. No evidence of infection with porcine endogenous retrovirus in recipients of porcine islet-cell xenografts. Lancet. 29;352(9129):695-699
  7. Kenji, H., Shuji, M., Takayuki, M., Junko, Y., Keizo, T., Mitsunori, O., Hikaru, M. and Ryota, S. 2003. The significance of N-linked glycosylation in pig endogenous retrovirus infectivity. Biochemical. Biophysical Research Communications 310:327-333 https://doi.org/10.1016/j.bbrc.2003.08.142
  8. Klymiuk, N., Muller, M., Brem, G. and Aigner, B. 2002. Characterization of porcine endogenous retrovirus gamma pro-pol nucleotide sequences. J Virol 76: 11738-11743 https://doi.org/10.1128/JVI.76.22.11738-11743.2002
  9. Klymiuk, N., Muller, M., Berm, G. and Aigner, B. 2003. Recombination analysis of human-tropic porcine endogenous retroviruses. J. Gen. Virol. 84:2729-2734 https://doi.org/10.1099/vir.0.19284-0
  10. Magre, S., Takeuchi, Y. and Bartosch, B. 2003. Xenotransplantation and pig endogenous retroviruses. Rev Med Virol. 13:311-329 https://doi.org/10.1002/rmv.404
  11. Mollnes, T. E. and Fiane, A. E. 1999. Xeno- transplantation : how to overcome the complement obstacle? Mol Immunol. 36:269-769 https://doi.org/10.1016/S0161-5890(99)00050-4
  12. Oldmixon, B. A., Wood, J. C., Ericsson, T. A., Wilson, C. A., White-Scharf, M. E., Andersson, G., Greenstein, J. L., Schuurman, H. J. and Patience, C. 2002. Porcine endogenous retrovirus transmission characteristics of an inbred herd of miniature swine. J Virol. 76(6):3045-30482 https://doi.org/10.1128/JVI.76.6.3045-3048.2002
  13. Patience, C., Takeuchi, Y. and Weiss, R. A. 1997. Infection of human cells by an endogenous retrovirus of pigs. Nat Med. 3:282-286 https://doi.org/10.1038/nm0397-282
  14. Patience, C., Patton, G. S., Takeuchi, Y., Weiss, R. A., McClure, M. O., Rydberg, L. and Breimer, M. E. 1998. No evidence of pig DNA or retroviral infection in patients with short-term extracorporeal connection to pig kidneys. Lancet. 29;352(9129): 699-701
  15. Stefan, J. T., Volker, S. and Joachim, D. 2003. Differences in Release and Determination of Subtype of Porcine Endogenous Retroviruses Produced by stimulated Normal Pig Blood Cells. Intervirol 46:17-24 https://doi.org/10.1159/000068120
  16. Steffi, B., Claire, A. and Andre, J. 2000. Study of Full-Length Porcine Endogenous Retrovirus Genomes with Envelope Gene Polymorphism in a Specific- Pathogen-Free Large White Swine Herd. J. Virol 74(18):8575-8581 https://doi.org/10.1128/JVI.74.18.8575-8581.2000
  17. Swindle, M. M. 1998. Defining appropriate health status and management programs for specific- pathogen-free swine for xenotransplantation. Ann. N. Y. Acad. Sci. 862:111-120 https://doi.org/10.1111/j.1749-6632.1998.tb09123.x
  18. Tacke, S. J., Kurth, R. and Denner, J. 2000. Porcine endogenous retroviruses inhibit human immune cell function: risk for xenotransplantation. Virol. 268: 87-93 https://doi.org/10.1006/viro.1999.0149
  19. Takeuchi, Y., Patience, C., Magre, S., Weiss, R. A., Banerjee, P. T., Le Tissier, P. and Stoye, J. P. 1998. Host range and interference studies of three classes of pig endogenous retrovirus. J Virol 72: 9986-9991
  20. Van de Peer, Y. and De Wachter, R. 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput. Applic. Biosci. 10:569-570
  21. Weiss, R. A. 1998. Transgenic pigs and virus adaptation. Nature 391:327-328 https://doi.org/10.1038/34772
  22. Wilson, C. A, Wong, S., Muller, J., Davidson, C. E., Rose, T. M. and Burd, P. 1998. Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells. J. Virol. 72: 3082-3087
  23. White, S. A. and Nicholson, M. L. 1999. Xen- otransplantation. Br. J. Surg. 86:1499-1514 https://doi.org/10.1046/j.1365-2168.1999.01340.x
  24. Wood, J. C., Quinn, G., Suling, K. M., Oldmixon, B. A., Van Tine, B. A., Cina, R., Am, S., Huang, C. A., Scobie, L., Onions, D. E., Sachs, D. H., Schuurman, H. J., Fishman, J. A. and Patience, C. 2004. Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature Swine. J Virol 78(5):2494-501 https://doi.org/10.1128/JVI.78.5.2494-2501.2004
  25. 김영봉, 유재영, 이종영, 김계웅, 박홍양. 2004. Prevalence of PERVs from Domestic Pigs in Korea (pol gene sequences) 한국동물자원과학회지 46(3): 307-314