• 제목/요약/키워드: Molecular Breeding

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Strategies for Improving Potassium Use Efficiency in Plants

  • Shin, Ryoung
    • Molecules and Cells
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    • 제37권8호
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    • pp.575-584
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    • 2014
  • Potassium is a macronutrient that is crucial for healthy plant growth. Potassium availability, however, is often limited in agricultural fields and thus crop yields and quality are reduced. Therefore, improving the efficiency of potassium uptake and transport, as well as its utilization, in plants is important for agricultural sustainability. This review summarizes the current knowledge on the molecular mechanisms involved in potassium uptake and transport in plants, and the molecular response of plants to different levels of potassium availability. Based on this information, four strategies for improving potassium use efficiency in plants are proposed; 1) increased root volume, 2) increasing efficiency of potassium uptake from the soil and translocation in planta, 3) increasing mobility of potassium in soil, and 4) molecular breeding new varieties with greater potassium efficiency through marker assisted selection which will require identification and utilization of potassium associated quantitative trait loci.

The Complete Chloroplast Genome Sequence and Intra-Species Diversity of Rhus chinensis

  • Kim, Inseo;Park, Jee Young;Lee, Yun Sun;Joh, Ho Jun;Kang, Shin Jae;Murukarthick, Jayakodi;Lee, Hyun Oh;Hur, Young-Jin;Kim, Yong;Kim, Kyung Hoon;Lee, Sang-Choon;Yang, Tae-Jin
    • Plant Breeding and Biotechnology
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    • 제5권3호
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    • pp.243-251
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    • 2017
  • Rhus chinensis is a shrub widely distributed in Asia. It has been used for traditional medicine and ecological restoration. Here, we report the complete chloroplast genome sequence of two R. chinensis genotypes collected from China and Korea. The assembled chloroplast genome of Chinese R. chinensis is 149,094 bp long, consisting of a large single copy (97,246 bp), a small single copy (18,644 bp) and a pair of inverted repeats (16,602 bp). Gene annotation revealed 77 protein coding genes, 30 tRNA genes, and 4 rRNA genes. A phylogenomic analysis of the chloroplast genomes with 11 known complete chloroplast genomes clarified the relationship of R. chinensis with the other plant species in the Sapindales order. A comparative chloroplast genome analysis identified 170 SNPs and 85 InDels at intra-species level of R. chinensis between Chinese and Korean collections. Based on the sequence diversity between Korea and Chinese R. chinensis plants, we developed three DNA markers useful for genetic diversity and authentication system. The chloroplast genome information obtained in this study will contribute to enriching genetic resources and conservation of endemic Rhus species.

Potential Biotypes in Korean Isolates of Bipolaris cactivora Associated with Stem Rot of Cactus

  • Kim, Jeong-Ho;Jeoung, Myoung-Il;Hyun, Ick-Hwa;Kim, Young-Ho
    • The Plant Pathology Journal
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    • 제20권3호
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    • pp.165-171
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    • 2004
  • A total of 62 isolates of Bipolaris cactivora causing cactus stem rots were isolated from major cactus-growing areas in Korea. Colony morphology of the isolates on potato-dextrose agar was differentiated into aerial (CA) and non-aerial mycelial types (CB). CA had profound aerial mycelium with grayish brown (CA-l), light brownish (CA-2), and brownish (CA-3) pigmentations; respectively, while CB had dark brownish pigmentations. CA had conidia of less dark pigmentation and acute terminal end. CB had darker and more round-end conidia. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of 2 random primers. The sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The isolates were classified into 2 major genomic DNA random amplified polymorphic DNA (RAPD) groups at the genomic similarity of 97.7% and 95.1%, respectively. Cluster analysis of genetic similarity among the isolates generated a dendrogram that clearly separated all isolates into SA or SB. This result suggests that there may be two morphotypes of B. cactivora in Korea that may differ in their genetic constitutes.

The Status of Seabirds in Korea and Environmental Monitoring Methods using Seabirds

  • Kim, Mi-Ran;Lee, Won-Choel;Zubrzycki, Igor Z.
    • 환경생물
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    • 제29권3호
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    • pp.113-125
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    • 2011
  • Seabirds have adapted to life in marine environments. More than 25% of the bird species observed in South Korea are seabirds, using the coast area of Korean peninsula as a stop-over and wintering, and breeding site. The aims of this review are to provide information about migratory and resident Korean seabirds and to discuss the methods that are currently employed to monitor the marine environment. In Korea, it has been reported that more than 400,000 individuals of seabirds breed on Nando Islet, Chilbaldo Islet, Guguldo Islet, Sasudo Islet, Hongdo Islet and Dokdo Islet. In 2010, approximately 160,000 seabirds also visited South Korea during the winter. Two of the main treats were introduced wildlife and habitat destruction by humans. Seabirds are monitored mainly at the population and individual levels. The assessment of population sizes and biomagnifications of pollutants are performed preferably at the community and population levels. Behaviour, growth, morphological characteristics, and breeding success is analyzed at the individual level and employed to gauge the health of the marine environment. In addition, we could suggest that molecular technique of seabirds successfully adopted to investigate the effects of pollutants and toxins in the marine environment.

Hypersensitive and Apoptotic Responses of Pepper Fruit Against Xnthomonas axonopodis pv. glycines Infection

  • Chang, Sung-Pae;Kim, Young-Ho
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.72.1-72
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    • 2003
  • Generally, plants defend themselves against pathogens by structural and biochemical reactions. Defense structures act as physical barriers and inhibit the pathogen from gaining entrance and spreading through the plant. Xanthomonas axonopodis pv glycines, the causal pathogen of bacterial pustule of soybean, causes hypersensitive response (HR). When pepper fruits were inoculated with X. axonopodis pv. glycines, in situ, time-series defense-related structural changes occurred in the inoculated sites. Early responses were programmed cell death (PCD), characterized by condensation and vacuolization of the cytoplasm, condensation of nuclear materials, and fragmentation of the nuclear DNA, which were observed by transmission electron microscopy. Nuclear fragmentation was proven by TUNEL method under confocal laser scanning microscopy and DNA laddering through eletrophoresis. At later stages, plant responses were cell elongation and cell division, forming a periderm-like boundary layer that demarcated healthy tissues from the inoculation sites. Using several stains such as toluidine blue, sudan IV, annexin V, and phloroglucinol-HCl, defense-related materials and structural changes were also examined.

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21세기 식물생명공학과 생물산업의 전망 : 유전체 연구에 의한 Paradigm Shift (Prospects for Plant Biotechnology and Bioindustry in the 21st Century: Paradigm Shift Driven by Genomics)

  • 유장렬;최동욱;정화지
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2002년도 추계학술대회
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    • pp.19-25
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    • 2002
  • Biotechnology in the 21st century will be driven by three emerging technologies: genomics, high-throughput biology, and bioinformatics. These technologies are complementary to one another. A large number of economically important crops are currently subjected to whole genome sequencing. Functional genomics for determining the functions of the genes comprising the given plant genome is under progress by using various means including phenotyping data from transgenic mutants, gene expression profiling data from DNA microarrays, and metabolic profiling data from LC/mass analysis. The aim of plant molecular breeding is shifting from introducing agronomic traits such as herbicide and insect resistance to introducing quality traits such as healthful oils and proteins, which will lead to improved and nutritional food and feed products. Plant molecular breeding is also expected to aim to develop crops for producing human therapeutic and industrial proteins.

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채소 품종 개량에 있어서 전통기술의 한계 극복을 위한 분자유전학의 역할 기대 (Bottlenecks of conventional approaches and complemental expectations of molecular biology in variental improvement of vegetable crops)

  • 윤진영;오대근
    • 한국식물학회:학술대회논문집
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    • 한국식물학회 1995년도 제9회 식물생명공학 심포지움 식물육종과 분자생물학의 만남 The 9th Plant Biotechnology Symposium -Breeding and Molecular Biology-
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    • pp.109-130
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    • 1995
  • 지난 반세기간에 우리나라에 채소 육종은 주요 채소의 주년공급을 가능하게 하였으며, 토지 생산성의 향상, 상품화율의 증대, 품질의 향상 등의 면에서도 괄목할 만한 성과를 거두었고 인공교잡은 물론이고 웅성불임성과 자가불화합성의 활용에 의한 1대잡종 품종의 일반화로 채소 산업의 발전에 크게 기여하였다. 앞으로는 기왕의 업적을 심화시키는 한편, 생산비를 절감하기 위한 생력화, 기계화 재배용 품종 및 내제초제성 품종의 개발 환경보호 및 식품안정성의 확보를 위한 내병층성 품종 개발, 수출시장과 다양화하는 국내의 시장기호에 대응하고 가공 식품의 표준화된 품질관리를 지원할 수 있도록 품질 면에서의 개량과 신작물 또는 신생태형 품종의 개발에도 더욱 노력이 필요하다. 이러한 육종목표를 달성하기 위한 유전자원의 확보는 더욱 어려워질 것이며 유전 양식이 복잡하고, 환경요인의 작용이 상대적으로 크기 때문에 전통적인 육종 방법만으로는 목표달성에 필요한 인적, 물적, 시간적 소요가 훨씬 증가될 전망이다. 유전변이의 창성 및 확대, 유용 대립인자의 도입, 동정 및 선발, 그리고 종자생산을 위한 자가 불화합성 및 웅성불임성과 개화·수정 관련 유전인자의 발현 조절에 분자유전학의 보완적 역할이 기대되며 이렇게 되면 전통육종과 분자유전학간의 잡종강세로 품종 개량의 효율은 크게 높아질 것이다.

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