• 제목/요약/키워드: Molecular Breeding

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주요 가축들의 유용유전자 및 질병관련 유전자 연구현황 (Current Research Status for Economically Important and Disease Related Genes in Major Livestock Species)

  • 이준헌;박찬규
    • Journal of Animal Science and Technology
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    • 제47권3호
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    • pp.325-340
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    • 2005
  • Great success has been being achieved in identification and utilization of candidate genes affecting economically important as well as disease related traits in animals for the past two decades. This article reviews the current status of candidate genes in animals, with particular emphasis on the disease causing genes in livestock. Two different types of phenotypes, monogenic and polygenic, are also described. Finally, commercialized animal DNA markers, which can be used for the molecular breeding in near future, are discussed.

형질전환 톨 페스큐 개발의 최근 동향 (Recent advance in genetic transformation of tall fescue)

  • 이기원;이상훈;김경희;이병현
    • Journal of Plant Biotechnology
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    • 제36권4호
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    • pp.336-343
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    • 2009
  • Tall fescue is an open-pollinated, perennial, cool season grass species widely used for forage and turf. Tremendous progress has been made in genetic transformation of tall fescue in the past decade. Methods for generating transgenic tall fescue plants have been developed based on biolistic transformation and Agrobacterium-mediated transformation. Potentially useful agronomic genes have been tested to environmental stress tolerance, herbicide tolerance and improve forage quality in tall fescue plants. We review progress in biotechnological improvement of tall fescue and discuss future molecular breeding of this species.

First Report of Sweet potato latent virus and Sweet potato chlorotic stunt virus Isolated from Sweet Potato in Korea

  • Yun, W.S.;Lee, Y.H.;Kim, K.H.
    • The Plant Pathology Journal
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    • 제18권3호
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    • pp.126-129
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    • 2002
  • Infected sweet potato (Ipomoea batatas) showing symptoms of sunken veins, stunting, mosaic, and mottling were collected from Gimje, Cochang, Iksan, and Haenam provinces in Korea. Electron microscopic (EM) observation of the infected tissue revealed rod and filamentous rod type virus particles of various lengths. Western blot analysis of the protein samples extracted from infected sweet potato and partially purified virus identified the isolates as Sweet potato feathery motile virus (SPFMV), Sweet potato latent virus (SwPLV), and Sweet potato chlorotic stint virus (SPCSV). Sweet potatoes were occasionally infected with more than one of these viruses. This is the first report of SwPLV and SPCSV in Korea.

The Divergent Roles of STAYGREEN (SGR) Homologs in Chlorophyll Degradation

  • Sakuraba, Yasuhito;Park, So-Yon;Paek, Nam-Chon
    • Molecules and Cells
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    • 제38권5호
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    • pp.390-395
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    • 2015
  • Degradation of chlorophyll (Chl) by Chl catabolic enzymes (CCEs) causes the loss of green color that typically occurs during senescence of leaves. In addition to CCEs, STAYGREEN1 (SGR1) functions as a key regulator of Chl degradation. Although sgr1 mutants in many plant species exhibit a staygreen phenotype, the biochemical function of the SGR1 protein remains elusive. Many recent studies have examined the physiological and molecular roles of SGR1 and its homologs (SGR2 and SGR-LIKE) in Chl metabolism, finding that these proteins have different roles in different species. In this review, we summarize the recent studies on SGR and discuss the most likely functions of SGR homologs.

Lipid accumulation mediated by adiponectin in C2C12 myogenesis

  • Yin, Changjun;Long, Qinqiang;Lei, Ting;Chen, Xiaodong;Long, Huan;Feng, Bin;Peng, Yin;Wu, Yanling;Yang, Zaiqing
    • BMB Reports
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    • 제42권10호
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    • pp.667-672
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    • 2009
  • Plasma concentrations of adiponectin have been shown to be decreased in patients with obesity, cardiovascular diseases, hypertension and metabolic syndrome. Recent studies have found that adiponectin reduces lipid accumulation in macrophage foam cells which may impact the development of atherosclerosis. However, it remains unclear whether adiponectin is involved in the process of lipid accumulation during myogenesis. Using C2C12 myoblasts, we investigated the effect of adiponectin on intramyocellular lipid accumulation during myogenesis. The results showed that intracellular lipid accumulation is significantly decreased during C2C12 differentiation, apparently due to increased fatty acid oxidation and decreased fatty acid synthesis during this process. C2C12 cells transiently transfected with adiponectin gene showed reduced lipid accumulation as compared to controls. Further experiments demonstrated that adiponectin can suppress lipid accumulation by increasing fatty acid oxidation during C2C12 myogenesis.

Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells

  • Li, Bo-jiang;Li, Ping-hua;Huang, Rui-hua;Sun, Wen-xing;Wang, Han;Li, Qi-fa;Chen, Jie;Wu, Wang-jun;Liu, Hong-lin
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권8호
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    • pp.1171-1177
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    • 2015
  • The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.