• 한국환경농학회지
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• 제23권4호
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• pp.245-250
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• 2004

An analytical method was developed to determine monocrotophos residues in apple, citrus, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Monocrotophos was extracted with acetone from apple, citrus and moist soil samples. The extract was concentrated, added with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives. Dichloromethane partition was then followed to recover monocrotophos from the aqueous phase. Silica gel column chromatography was employed to further purify the extract prior to HPLC determination. Reverse-phase HPLC using an oct-adecylsilyl column was successfully applied to separate and quantitate the monocrotophos residue in sample extracts at the wavelength of 230 nm. Overall recoveries of monocrotophos from fortified samples averaged $95.3{\pm}2.1%$ (n=6), $970{\pm}0.7%$ (n=6), and $92.8{\pm}4.3%$ (n=12) for apple, citrus, and soil, respectively. The proposed method was quite reproducible and sensitive enough to replace the troublesome gas-liquid chromatographic analysis for monocrotophos residues.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1559-1567
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• 2012

The objective of this study was to evaluate ruminal degradability and intestinal digestibility of crude protein (CP) and amino acids (AA) in hempseed cake (HC) that were moist heat treated at different temperatures. Samples of cold-pressed HC were autoclaved for 30 min at 110, 120 or $130^{\circ}C$, and a sample of untreated HC was used as the control. Ruminal degradability of CP was estimated, using the in situ Dacron bag technique; intestinal CP digestibility was estimated for the 16 h in situ residue using a three-step in vitro procedure. AA content was determined for the HC samples (heat treated and untreated) of the intact feed, the 16 h in situ residue and the residue after the three-step procedure. There was a linear increase in RUP (p = 0.001) and intestinal digestibility of RUP (p = 0.003) with increasing temperature during heat treatment. The $130^{\circ}C$ treatment increased RUP from 259 to 629 g/kg CP, while intestinal digestibility increased from 176 to 730 g/kg RUP, compared to the control. Hence, the intestinal available dietary CP increased more than eight times. Increasing temperatures during heat treatment resulted in linear decreases in ruminal degradability of total AA (p = 0.006) and individual AA (p<0.05) and an increase in intestinal digestibility that could be explained both by a linear and a quadratic model for total AA and most individual AA (p<0.05). The $130^{\circ}C$ treatment decreased ruminal degradability of total AA from 837 to 471 g/kg, while intestinal digestibility increased from 267 to 813 g/kg of rumen undegradable AA, compared with the control. There were differences between ruminal AA degradability and between intestinal AA digestibility within all individual HC treatments (p<0.001). It is concluded that moist heat treatment at $130^{\circ}C$ did not overprotect the CP of HC and could be used to shift the site of CP and AA digestion from the rumen to the small intestine. This may increase the value of HC as a protein supplement for ruminants.

• Applied Biological Chemistry
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• 제41권8호
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• pp.595-599
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• 1998

An analytical method was developed to determine tricyclazole residues in rice grain, straw, and soil using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. Tricyclazole was extracted with methanol from moist rice grain, straw, and soil samples. n-Hexane washing was employed to remove nonpolar co-extractives during liquid-liquid partition. Tricyclazole was then extracted with dichloromethane from alkaline aqueous phase, while acidic interferences remained in the phase. Dichloromethane extract was further purified by silica gel column chromatography prior to HPLC determination. Reverse-phase HPLC using an octadecylsilyl column was successfully applied to separate and quantitate the tricyclazole residue in sample extracts monitored at ${\lambda}_{max}$ 225nm. Recoveries from fortified samples averaged $95.5{\pm}3.0%\;(n=6),\;87.5{\pm}20.%\;(n=6),\;and\;84.3{\pm}2.8%$ (n=12) for rice grain, straw, and soil, respectively. Detection limit of the method was 0.02 mg/kg for rice grain and soil samples while 0.05 mg/kg for rice straw samples. The proposed method was reproducible and sensitive enough to evaluate the safety of tricyclazole residues in rice grain, straw, and soil.

• Agricultural and Biosystems Engineering
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• 제2권2호
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• pp.63-68
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• 2001

The objective of this study was to develop a real-time soil moisture meter using RF impedance. The impedance suchas capacitance and resistance (or conductance) was analyzed using parallel cylinder type capacitance probe(C-probe) and Q-meter (HP4342). The capacitance and conductance of soil increased as volumetric water content increased. The 5 MHz of modified Colpitts type crystal oscillator was designed to detect the capacitance change of the C-probe with moist soil. A third order polynomial regression model was proposed to describe the relationship between RF impedance and volumetric water content. The prototype real time moisture meter consisted of the C-probe, sample container, oscillator, frequency counter and related signal processing units. The calibration equation for measurement of volumetric moisture content of soil was developed and validated. The correlation coefficient and root mean square error between measured volumetric water content by oven method and predicted values by prototype moisture meter for unknown soil samples were 0.984 and 0.032$cm^3$$cm\^3$, respectively.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.200-200
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• 2017

Salinity stress is one of the most important abiotic stresses and severely impairs plant growth and production. Root is the first site for nutrient accumulation like as $Na^+$ in the plant. To investigate the response of wheat root under salinity stress, we executed the characterization of morphology and analysis of metabolites. Wheat seeds cv. Keumgang (Korean cultivar) were grown on the moist filter paper in Petri dish. After 5 days, seedlings were transferred to hydroponic apparatus at 1500 LUX light intensity, at $20^{\circ}C$ with 70% relative humidity in a growth chamber. Seedlings (5-day-old) were exposed to 50mM, 75mM, 100mM NaCl for 5 days. Ten-day-old seedlings were used for morphological characterization and metabolite analysis. Root and leaf length became shorter in high NaCl concentration compared to following NaCl treatment. For confirmation of salt accumulation, wheat roots were stained with $CoroNa^+$ Green AM, and fluoresce, and the image was taken by confocal microscopy. $Na^+$ ion accumulation rate was higher at 100mM compared to the untreated sample. Furthermore, to analyze metabolites in the wheat root, samples were extracted by $D_2O$ solvent, and extracted sample was analyzed by 1H NMR spectroscopy. Fourteen metabolites were identified in wheat roots using NMR spectroscopy. Methanol and ethanol were up-regulated, whereas formate, aspartate, aminobutyrate, acetate and valine were down-regulated under salinity stress on roots of wheat. Fumarate had no change, while glucose, betaine, choline, glutamate and lactate were unevenly affected during salinity stress.

• 한국산림과학회지
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• 제38권1호
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• pp.1-12
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• 1978

휴면기간(休眠期間)이 긴 은행나무 종자(種子)의 냉습적처리(冷濕積處理)에 따른 생장촉진물질(生長促進物質)과 생장억제물질(生長抑制物質)의 증감(增減)을 구명(究明)하기 위하여 본(本) 연구(硏究)를 수행(遂行)하고 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. 무처리종자(無處理種子)는 배(胚), 배유(胚乳), 종피(種皮)의 각추출물(各抽出物)에서 생장억제물질(生長抑制物質)을 볼 수 있었으며, 특(特)히 종피(種皮)에서 가장 현저(顯著)하게 나타났다. 2. 냉습적처리종자(冷濕積處理種子)의 배(胚)와 종피(種皮)에서 생장억제물질(生長抑制物質)의 현저(顯著)한 감소(減少)가 있었으나, 생장촉진물질(生長促進物質)은 배부분(胚部分)에서 증가(增加)가 약간 나타났다. 3. 이상(以上)의 결과(結果)에서 은행나무 종자(種子)의 휴면(休眠)에는 종피(種皮), 배(胚), 배유(胚乳)의 각부분(各部分)이 생장억제물질(生長抑制物質)이 존재(存在)하며 발아(發芽)에 관계하는 것으로 추측(推測)된다. 4. 은행나무 종자(種子)와 발아촉진(發芽促進)과의 관계는 생장촉진물질(生長促進物質)의 증가(增加)보다는, 배(胚)와 종피(種皮)의 생장억제물질(生長抑制物質)의 감소(減少)에 영향된다고 추측(推測)된다. 5. 생장촉진물질(生長促進物質)인 Gibberellin은 자외선형광검사등검정(紫外線螢光檢査燈檢定)에서 Rf 0.26, 에서 나타났고, 생장억제물질(生長抑制物質)인 Abscisic acid는 Rf 0.62, Rf 0.70, Rf 0.78에서 각각(各各) purple, gray, blue의 형광색(螢光色)으로 나타났다.

• 한국가금학회지
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• 제28권3호
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• pp.215-224
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• 2001

질소(N)는 그 자체가 환경적인 요인에 따라 영향을 받는 휘발성 물질이어서 가축의 분뇨(분에는 반드시 뇨가 묻어 있기 때문에 다음부터는 분이라는 말로 대치한다)중에 함유되어 있는 N의 함량을 정량하기란 무척 어렵다. 우선 가축의 분이 실험실에 도착하면 수분이 있는 상태에서 냉장고에 보관되어야 한다. 분중에 함유되어 있는 N는 건조 온도에 따라 함량에 변화를 일으키며 또 시료에 흙이 묻어 있는가에 따라서도 N의 함량 정량에 영향을 받는다. 시료를 산성화하면 암모니아의 휘발이 방해된다. 따라서 시료를 산성화시킬 때는 건조전에 이루어져야 한다. 분 분석을 위한 이상적인 분 전처리는 강산화제 ($KMnO_{4}$ )로 우선 처리 한 후 다시 환원제 (Fe- $H_{2}$ $SO_{4}$)를 처리한 상태에서 시료를 소화시키면 가장 정량하기 어려운 $NH_{4}$ -N도 같이 정량이 가능하며 질소의 전체정량이 정확히 이루어질 수 있다. 질소정량의 정확성이 야외에서 이루어질 수 있도록 최근 여러 가지의 약식분석방법이 개발되고 있지만 그 결과는 반드시 공인된 분석방법에 의하여 실험실에서 분석된 결과치와 비교되어야 한다. 실험실에서 일반적으로 질소 정량에 많이 이용되고 있는 방법은 켈달 분석방법이다. 앞으로 가축의 종류나 품종, 나이 또는 개체간에 대한 분의 영양소 함량에 관한 연구가 많이 이루어져야 한다. 또 분에 대한 농장에서의 시료 채취과정, 운반, 및 실험실에 도착한 후 처리 과정 그 다음 실험실에서 분석을 위한 시료채취과정 등에 대한 연구도 이루어져야 한다. 사료, 분뇨, 토양, 그리고 물을 함께 분석하는 동물농업의 발전을 필요하며 적당한 전문가의 이용이 필요하다.

• 한국조리학회지
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• 제22권8호
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• pp.226-234
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• 2016

본 연구는 우리나라 야산에서 쉽게 구할 수 있는 구황 식물인 질경이를 양갱 제품 개발을 위해 질경이 분말을 5~20% 첨가하여 양갱을 제조한 다음 이화학적 및 관능적 특성을 평가하여 새로운 양갱 제품 개발 가능성을 살펴보았다. 수분함량은 1.9%로 나타났으며, DPPH 라디칼 소거능에서는 15.67 mg/mL, 총 폴리페놀 함량은 7.00 mg/g의 값을 보였다. 질경이 첨가량에 따른 양갱의 수분과 pH는 첨가량이 증가할수록 낮은 값을 보였다. 색도는 첨가량이 증가할수록 명도 L값은 감소하였고, 적색도 a값과 황색도 b값도 첨가량이 증가할수록 감소하는 경향을 보였으며 a값인 5% 첨가구와 20% 첨가구에서 가장 높은 값과 낮은 값으로 나타내었다(p<0.001). 텍스쳐에서 견고성은 질경이 분말 첨가량 5% 첨가구에서 3,937.04로 가장 높은 값을 보였고, 20% 첨가구에서 2,153.59로 낮은 값을 나타내었다. 탄력성에서는 5% 첨가구에서 6.79로 가장 높은 값을 나타내었으며, 20% 첨가구에서 4.76%으로 낮은 값을 나타내었다. 응집성에서는 20% 첨가구가 177.35로 가장 낮은 값을 보였으며 유의적이었다(p<0.001). 관능검사 결과 색, 향, 단맛, 씹힘성, 촉촉한 정도, 부드러운 정도, 전체적인 기호도 등에서 질경이 분말 첨가량에 따른 양갱은 10% 첨가군이 가장 높게 평가되었다. 이상의 결과로 질경이 분말 양갱 제조 시 적정량의 첨가는 관능적인 기호도를 상승시킬 수 있으므로 질경이 분말 첨가 양갱의 제품화 가능성이 좋다고 할 수 있다. 관능기호도 측면에서 질경이 분말은 10%가 가장 적합하리라 판단되었다.

• 한국식품영양학회지
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• 제20권4호
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• pp.399-405
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• 2007

The quality characteristics of breads made by adding levels of 30%, 60%, and 100% aloe gel were investigated. The 60% aloe gel sample had a larger dough volume than the control dough. Also, the loaf volume and specific volume of the bread made with 60% aloe gel were larger than those of the control bread. The pasting temperature increased gradually with the increasing amounts of substituted aloe gel as compared to wheat flour with water added$(66.5{\pm}12^{\circ}C)$; the wheat flour with 100% aloe gel had a pasting temperature of $90.7{\pm}1.1^{\circ}C$. Peak viscosity decreased gradually with increasing amounts of aloe gel when compared to the wheat flour with water added. Setback also was decreased gradually with the increasing amounts of aloe gel as compared to the wheat flour with added water. From the setback decrease it is suggested that the aloe controlled retrogradation of the bread during short-term storage. The crumb color of the bread made with the aloe gel was not significantly different to that of the control bread, and the color of the crumb was yellow-white. Bread hardness decreased gradually with increasing amounts of the substituted aloe gel. However, bread gumminess and chewiness increased gradually with increasing amounts of aloe gel. The springiness of the bread made with 100% aloe gel was lower than that of the control bread. With regard to flavor and taste, the bread made with 100% aloe gel produced a green-like odor and had a bitter taste. The mouth feel of the breads made with aloe gel was considered soft and moist. The overall acceptabilities of the breads made with 30% and 60% aloe gel were not significantly different from the control bread, but the bread made with 100% aloe gel had a green smell and bitter taste. Yet accordingly, the results indicate that functional and health products with improved quality could be developed by adding aloe gel to breads and cakes.

• 한국식품과학회지
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• 제33권2호
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• pp.216-220
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• 2001

이 연구에서는 산화 셀룰로오스가 케이크 등과 같은 식품에서 식이섬유 소재로 사용될 수 있는지 그 가능성을 조사하기 위하여, TEMPO, NaBr 및 NaOCl을 동시에 사용하여 셀룰로오스 내의 1차 알코올기를 산화시키고, 산화 셀룰로오스를 밀가루 함량의 10%수준으로 대체하여 제조한 케이크의 이화학적 및 관능적 특성을 평가하였다. 그 결과 산화 셀룰로오스를 함유한 케이크는 산화 셀룰로오스를 첨가하지 않은 대조군보다 전체적으로 색이 어둡고 노란색이 강하였으나, 전체적인 부피는 증가하는 것으로 나타났다. 관능적 평가 결과 셀룰로오스를 첨가한 케이크는 가루끼 특성이 강하게 나타난 반면, 산화 셀룰로오스를 첨가한 케이크는 대조군과 가루끼 특성에서는 유의적인 차이를 보이지 않았고, 단단한 정도는 적고 촉촉한 정도는 더 크게 나타나, 산화 셀룰로오스를 케이크에 첨가시 텍스처 특성이 오히려 향상되는 것으로 나타났다. 이 실험 결과 산화 셀룰로오스는 케이크에서 외관이나 텍스처 특성의 저하없이 식이섬유 소재로 사용될 수 있음을 알 수 있었다.