• The Korean Journal of Physiology and Pharmacology
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• v.9 no.5
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• pp.275-282
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• 2005

By using differential display, we identified one of the genes encoding the multi-subunit complex protein V-ATPase, c subunit gene (ATP6L), and showed alterations of the gene expression by oxidative stresses. Expression of the ATP6L gene in Neuro-2A cells was increased by the treatment with $H_2O_2$ and incubation in hypoxic chamber, implying that the expression of the ATP6L gene is regulated by oxidative stresses. To examine mechanisms involved in the regulation of the gene expression by oxidative stresses, the transcriptional activity of the rat ATP6L promoter was studied. Transcription initiation site was determined by primer extension analysis and DNA sequencing, and promoter of the rat ATP6L and its deletion clones were constructed in reporter assay vector. Significant changes of the promoter activities in Neuro-2A cells were observed in two regions within the proximal 1 kbp promoter, and one containing a suppressor was in -195 to -220, which contains GC box that is activated by binding of Sp1 protein. The suppression of promoter activity was lost in mutants of the GC box. We confirmed by electrophoretic mobility shift and supershift assays that Sp1 protein specifically binds to the GC box. The promoter activity was not changed by the $H_2O_2$ treatment and incubation in hypoxic chamber, however, $H_2O_2$ increased the stability of ATP6L mRNA. These data suggest that the expression of the ATP6L gene by oxidative stresses is regulated at posttranscriptional level, whereas the GC box is important in basal activities of the promoter.

• Science of Emotion and Sensibility
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• v.12 no.4
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• pp.461-470
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• 2009

This study has been prepared based on signboard arrangement works in order to foster Jeonju traditional Korean style house village as a main city of traditional culture. Overall composition has been suggested based on basic direction of signboard design in order to ensure design standardization method and utilization and right installation of various signboards. Enquete had been progressed by dividing into three areas including general characteristics of Korean style house village, acquisition of information and mobility and regulation/range of signboard with taking 600 number of persons from Jeonju citizens, outsiders and residents of Korean style house village as targets. Based on above result, design concept and detailed sign application method had been defined and pictogram and signboard installation method of Korean style house was suggested. As for the administrative procedure and regulations of signboard arrangement, cooperative method among Jeonju City, publicity company, deliberation committee and local residents and administrative project implementation method were suggested. Therefore, this study had summarized the project progress of signboard design for Jeonju traditional Korean style house village so that it may be utilized as a basic data for ensuring that this village would be developed as a region where tradition and history will be well conserved in the future through consultation with the relevant authorities.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.419-427
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• 2020

This study was undertaken to address the difficulties and inconveniences of an electric wheelchair. We focused on improving usability of initially completed products by augmenting the prototypes designed in the previous study. For evaluation of usability, 10 elderly women aged over 65 years, capable of movements and physical activities in daily life, were enrolled as subjects. The experimental method included a subjective satisfaction questionnaire evaluation of the elderly women using the target product, and the observation evaluation was achieved using video recording data, etc. Usability evaluation revealed that the elevating sector requires improvement of intuition through separation of the elevating control panel and the driving control panel. Improvements in the driving sector include corrections of the front wheel mechanism or driving control algorithm, UI, and sudden stop system. Transferring section assessment revealed a necessity to secure structures and add structures that support power. We believe that based on the inconveniences and improvements presented in the usability evaluation, appending the existing prototype with complementary products will improve the quality of life of elderly women with limited mobility.

• Asian Journal of Atmospheric Environment
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• v.11 no.4
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• pp.300-312
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• 2017

To improve the understanding of secondary organic aerosol (SOA) formation from the photo-oxidation of anthropogenic and biogenic precursors at the regional background station on Baengnyeong Island, Korea, gas phase and aerosol chemistries were investigated using the Proton Transfer Reaction Time of Flight Mass Spectrometer (PTR-ToF-MS) and the Aerodyne High Resolution Time of Flight Aerosol Mass Spectrometer (HR-ToF-AMS), respectively. HR-ToF-AMS measured fine particles ($PM_1$; diameter of particle matter less than $1{\mu}m$) at a 6-minute time resolution from February to November 2012, while PTR-ToF-MS was deployed during an intensive period from September 21 to 29, 2012. The one-minute time-resolution and high mass resolution (up to $4000m{\Delta}m^{-1}$) data from the PTR-ToF-MS provided the basis for calculations of the concentrations of anthropogenic and biogenic volatile organic compounds (BVOCs) including oxygenated VOCs (OVOCs). The dominant BVOCs from the site are isoprene (0.23 ppb), dimethyl sulphide (DMS, 0.20 ppb), and monoterpenes (0.38 ppb). Toluene (0.45 ppb) and benzene (0.32 ppb) accounted for the majority of anthropogenic VOCs (AVOCs). OVOCs including acetone (3.98 ppb), acetaldehyde (2.67 ppb), acetic acid (1.68 ppb), and formic acid (2.24 ppb) were measured. The OVOCs comprise approximately 75% of total measured VOCs, suggesting the occurrence of strong oxidation processes and/or long-range transported at the site. A strong photochemical aging and oxidation of the atmospheric pollutants were also observed in aerosol measured by HR-ToF-AMS, whereby a high $f_{44}:f_{43}$ value is shown for organic aerosols (OAs); however, relatively low $f_{44}:f_{43}$ values were observed when high concentrations of BVOCs and AVOCs were available, providing evidence of the formation of SOA from VOC precursors at the site. Overall, the results of this study revealed several different SOA formation mechanisms, and new particle formation and particle growth events were identified using the powerful tools scanning mobility particle sizer (SMPS), PTR-ToF-MS, and HR-ToF-AMS.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.18 no.2
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• pp.157-167
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• 2020

Removing radioactive concrete is crucial in the decommissioning of nuclear power plants. However, this process generates radioactive aerosols, exposing workers to radiation. Although large amounts of radioactive concrete are generated during decommissioning, studies on the internal exposure of workers to radioactive aerosols generated from the cutting of radioactive concrete are very limited. In this study, therefore, we calculate the internal radiation doses of workers exposed to radioactive aerosols during activities such as drilling and cutting of radioactive concrete, using previous research data. The electrical-mobility-equivalent diameter measured in a previous study was converted to aerodynamic diameter using the Newton-Raphson method. Furthermore, the specific activity of each nuclide in radioactive concrete 10 years after nuclear power plants are shut down was calculated using the ORIGEN code. Eventually, we calculated the committed effective dose for each nuclide using the IMBA software. The maximum effective dose of ¹⁵²Eu constituted 83.09% of the total dose; moreover, the five highest-ranked elements (¹⁵²Eu, ¹⁵⁴Eu, ⁶⁰Co, ²³⁹Pu, ⁵⁵Fe) constituted 99.63%. Therefore, we postulate that these major elements could be measured first for rapid radiation exposure management of workers involved in decommissioning of nuclear power plants, even if all radioactive elements in concrete are not considered.

• Journal of Satellite, Information and Communications
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• v.4 no.1
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• pp.1-7
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• 2009

As the information age comes out, the aspect of future war brings about the many changes in terms of war-fighting environment. Accordingly, information superiority and intelligence-centric warfare have been important and new war-fighting concept such as NCW(network centric warfare) have been turned up. This paper proposed all-weather core-strategy communications systems guaranteeing not only the real-time transmission of the information collected in a battlefield and expansion, automation, and rapidity of a battlefield but also broadband, mobility, survivability, and flexibility. The proposed military satellite communications system is classified into wideband mass capacity link, survivability, and the system supporting OTM(on the move) communication for the real-time transmission of battlefield information. This paper analyzed the essential operation concepts and core schemes of the U.S. Army's next generation system, TSAT(Transformational Satellite Communication System). Base on the analysis results, this paper proposed that the architecture of next generation military satellite communications systems for NCW have to provide the data rate, anti-jamming capability, network control and management capability which are optimally adaptable for the wireless channel environments such as jamming and interference and to support the variety of platforms like high-speed mobile vehicles, micromini devices, super-high speed unmanned aerial vehicles. Finally, this paper also proposed that next generation military satellite communications systems need the technologies such as the adaptable multi-antenna, laser link, and next-generation anti-jamming waveform.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.318-328
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• 2018

The purpose of this study was to investigate the differences in the characteristics of the aesthetic salon operation and job satisfaction according to important considerations in opening aesthetic salons as well as to provide basic data for improving the operation of the salons. Toward this aim, a questionnaire survey was conducted for owners of small aesthetic salons across South Korea from January-March, 2016. Results were as follows: Among those who identified means of transportation and parking convenience as key considerations in launching aesthetic salon businesses, length of service, length of business operation, monthly fixed revenue and proportion of the highest profit were highest. Job satisfaction (F=3.223, p<.05) was highest (M=4.11) among those that responded 'floating population' as a primary component in launching aesthetic salon businesses, and lowest among owners who mentioned 'cost of investment' (M=3.41). Specifically, job mobility (F=2.960, p<.05) was the highest among respondents who selected means of transportation and parking convenience (M=3.95) as key criteria in launching aesthetic salon business. For the stable operation of an aesthetic salon, a location with high floating population, good transportation, parking convenience, high levels of customer access, and job satisfaction are critical factors. In addition, future studies should examine marketing strategies by operating characteristics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.164-173
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• 2018

The purpose of this study is to investigate the effect of job experience while attending college on the turnover risk in the first job following graduation. Data was obtained from the 2015 Graduates Occupational Mobility Survey (GOMS). Subjects included college graduates under 30 years of age, who graduated from high school and entered college in the same year. The results of this study are as follows: First, students who had job experience while attending school were relatively low in economic characteristics, but showed active employment preparation behavior. Second, there was a significant difference in the characteristics of entry into the labor market according to whether they had job experience while attending school. It was found that the securing their first job of graduates with job experience was shorter, and the period of tenure was longer, but their wage was smaller than non-experienced graduates. Third, the Cox regression analysis confirmed what factors affected their employment period, and that job experience, experience frequency, and experience period while attending school had a positive effect on lowering turnover risk. Therefore, this study found that job experience during schooling years makes the employment period of the job longer after graduation, which is consistent with those who had previous job experience. However, the greater amount of job experience during the period of study positively affects job retention.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.152-161
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• 2015

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY^- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY^-, named BMB171cry1Ac and BMB171codY^-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY^-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY^-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.11
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• pp.2594-2603
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• 1997

A novel method of adaptive beam forming is presented in this paper. The proposed technique provides for a suboptimal beam pattern that increases the Signal to Noise/Interference Ratio (SNR/SIR), thus, eventually increases the capacity of the communication channel, under an assumption that the desired signal is dominant compared to each component of interferences at the receiver, which is precoditionally achieved in Code Division Multiple Access (CDMA) mobile communications by the chip correlator. The main advantages of the new technique are:(1)The procedure requires neither reference signals nor training period, (2)The signal interchoerency does not affect the performance or complexity of the entire procedure, (3)The number of antennas does not have to be greater than that of the signals of distinct arrival angles, (4)The entire procedure is iterative such that a new suboptimal beam pattern be generated upon the arrival of each new data of which the arrival angle keeps changing due tot he mobility of the signal source, (5)The total amount of computation is tremendously reduced compared to that of most conventional beam forming techniques such that the suboptimal beam pattern be produced at vevery snapshot on a real-time basis. The total computational load for generating a new set of weitht including the update of an N-by-N(N is the number of antenna elements) autocovariance matrix is $0(3N^2 + 12N)$. It can further be reduced down to O(11N) by approximating the matrix with the instantaneous signal vector.