• Radioisotope journal
• /
• v.21 no.4
• /
• pp.33-37
• /
• 2006

A fertilizer mixed with an electron beam irradiated sewage sludge and a starfish powder was developed. By the irradiation of sewage sledge, it could be hygienic from harmful microorganism and be free from Rely nucleonic Aromatic Hydrocarbons (PAHs). The quality of fertilizer mainly consisted of electron beam irradiated sewage sledge could be enhanced by addition of starfish powder rich in organic calcium ion. The growth rate for a grass and a rose tree was enhanced about 35% when a fertilizer applied. By the development of new type of fertilizer, a large amount of sewage sledge and star fish con be utilized as a fertilizer.

• Journal of Soil and Groundwater Environment
• /
• v.8 no.1
• /
• pp.27-34
• /
• 2003

In this study, potential degradation of MTBE and other gasoline oxygenates by pure culture ENV425 and mixed culture isolated from gasoline contaminated soil using butane as the sources of carbon and energy was examined and compared. Butane monooxygenases(BMO) of butane-grown ENV425 and mixed culture generated 1-butanol as a major metabolite of butane oxidation and addition of acetylene, specific inhibitor of monooxygenase, inhibited both butane oxidation and 1-butanol production. The results described in this study suggest that alkanes including propane, pentane, and butane are effectively utilized as a growth substrate to oxidize MTBE cometabolically. And also BTEX compounds could be the potential substrate of the MTBE cometabolism. Cell density also affected on the MTBE degradation and transformation capacity(Tc). Increasing cell density caused increasing MTBE degradation but decreased transformation capacity. Other result demonstrated that MTBE and other gasoline oxygenates, ETBE and TAME, were degraded by butane-grown microorganism.

• Microbiology and Biotechnology Letters
• /
• v.23 no.6
• /
• pp.702-709
• /
• 1995

Degradation Of $\kappa $-carrageenan-based film by microorganisms screened from carrageenan source and activated sludge of a carrageenan producing factory was investigated by measuring changes of pH, viscosity, total sugar and total organic carbon (TOC) of the medium containing $\kappa $-carrageenan as a carbon source. Initially fifteen strains of microorganism were isolated from carrageenan source and activated sludge and then three organisms among them were selected based on the ability of growing in the medium including 0.3% $\kappa $-carrageenan as a sole carbon source. They were identified as Escherichia coli, Saccharomyces cerevisiae and Aspergillus niger. As indices of biodegradability Of $\kappa $-carrageenan based film, the changes of pH, viscosity, total sugar, and TOC of the carrageenan film-based medium were tested by the cultivation of single or mixed strains of the identified organisms. Mixed culture showed the highest biodegradability, which resulted in the changes of pH from 6.5 to 3.0, viscosity from 164 cps to 15.6 cps, total sugar content from 2.35 g/l to 0.53 g/l and TOC from 5721 ppm to 232 ppm after 30 days of cultivation. The biodegradability determined as the reduction rate of TOC by pure cultures of Asp. niger, E. coli, Sacch. cerevisiae and mixed culture of the three organisms were 94%, 86%, 80% and 96%, respectively.

• Membrane and Water Treatment
• /
• v.6 no.6
• /
• pp.501-512
• /
• 2015

A laboratory-scale submerged membrane bioreactor (MBR) was continuously operated for 100 d at an infinite sludge retention time (SRT) with the aim of identifying possible relation between the filterability of mixed liquor and sludge properties, such as extracellular polymeric substances (EPS), soluble microbial products (SMP), viscosity of mixed liquor, zeta potential of flocs and particle size distributions (PSD). Research results confirmed that MBR can operate with a complete sludge retention ensuring good treatment performances for COD and $NH_3-N$. However, the long term operation (about 40 d) of MBR with no sludge discharge had a negative influence on sludge filterability, and an increase in membrane fouling rates with the time was observed. There as a strong correlation between the sludge filterability and the fouling rate. Among the different sludge properties parameters, the concentration SMP and EPS had a more closely correlation with the sludge filterability. The concentrations of SMP, especially SMP with MW above 10 kDa, had a strong direct correlation to the filterability of mixed sludge. The protein fractions in EPS were biodegradable and available for microorganism metabolism after about 60 days, and the carbohydrates in EPS had a significantly negative effect on sludge filterability in MBR at an infinite SRT.

• Membrane and Water Treatment
• /
• v.7 no.1
• /
• pp.11-22
• /
• 2016

The characterization of treatment performance with respect to mixed liquor suspended solids (MLSS) concentration enables greater control over system performance and contaminant removal efficiency. Hybrid membrane bioreactors (HMBRs) have yet to be well characterized in this regard, particularly in the context of greywater treatment. The aim of this study, therefore, was to determine the optimal MLSS concentration for a decentralized HMBR greywater reclamation system under typical loading conditions. Treatment performance was measured at MLSS concentrations ranging from 1000 to 4000 mg/L. The treated effluent was characterized in terms of biochemical oxygen demand ($BOD_5$), chemical oxygen demand (COD), turbidity, ammonia ($NH_3$), total phosphorus (TP), total kjeldahl nitrogen (TKN), and total nitrogen (TN). An MLSS concentration ranging from 3000 to 4000 mg/L yielded optimal results, with $BOD_5$, COD, turbidity, $NH_3$, TP, TKN, and TN removals reaching 99.2%, 97.8%, 99.8%, 99.9%, 97.9%, 95.1%, and 44.8%, respectively. The corresponding food-to-microorganism ratio during these trials was approximately 0.23 to 0.28. Operation at an MLSS concentration of 1000 mg/L resulted in an irrecoverable loss of floc, and contaminant residuals exceeded typical guideline values for reuse in non-potable water applications. Therefore, it is suggested that operation at or below this threshold be avoided.

• Journal of Biosystems Engineering
• /
• v.33 no.4
• /
• pp.253-259
• /
• 2008

In this paper an optimum design of a lab-scale biofilter for absorbing ammonia has been proposed and analyzed. This biofilter is using pine chaff and wood shaving as filter materials. It is assumed that the biofilter can be used as a storage tank of swine manure slurry or swine stall. To evaluate the biofilter performance, the ammonia, mainly offensive odor ingredient, was measured. Swine compost was mixed with filter materials in ratio of 1:1 on weight base. Each test continued for 20 days. The ammonia emissions were reduced by 97.9% and 98.3% in case of using biofilter filled with pine chaff and compost, and wood shaving and compost, respectively. The system was tested with and without adding compost. It was found that the biofilter with wood shaving and compost has an ammonia removal efficiency of 94.1%, while biofilter with wood shaving only has 85.3%. The biofilter with wood shaving and compost showed 8.8% higher removal efficiency than that of wood shaving only. By mixing the compost, the number of microorganism was found to be about 2.3 times more than that of wood shaving only. Therefore it can be concluded that adding compost has a positive effect on the formation of microorganism.

• Journal of the Korean GEO-environmental Society
• /
• v.18 no.10
• /
• pp.15-22
• /
• 2017

The soil improvement method so far has been developed with an emphasis on enhancing the strength of the ground. A soil improvement method using a excellent cementitious stabilizer in economical efficiency and handling property is mainly used. The soil improvement method using cementitious stabilizer is effective but environmental and human harmful substances are detected and environmental problems such as carbon dioxide emission and groundwater pollution are pointed out. Therefore, as part of an alternative method capable of solving such problems, researches on the soil improvement method incorporating biological technology are being actively carried out. This study was conducted to investigate the characteristics of strength change when mixed with environmentally friendly soil binder and microorganism in clay, and it was analyzed by uniaxial compression test, direct shear test, SEM, XRD. As a results of the test, we confirmed the cementation caused by microbially induced calcite precipitation and the strength increase enhancement by it.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.12 no.4
• /
• pp.72-80
• /
• 2009

The main purpose of this study is as follows : Finding a solution for fresh wood chips to be used as an alternative growing-media through a study of the formation method of a compost base that is applicable on both construction site and composting factory to ferment fresh wood chips produced from construction site as well as a study on adjuvant or secondary materials. The result from the experiment plot using wood chips sized 50mm or less, manure and fertilizer mixed manure as a source of nitrogen to compost fresh wood chips has shown that the temperatures 9days and 3days after the beginning of the experiment reached to $49.0^{\circ}C$ and $40.4^{\circ}C$ respectively, the heating duration was 7days and 4days respectively, and the C/N ratio was 26.5 and 25.3, each satisfying the standard for composted manure (25.0~40.0). Also, the other result from another experiment plot using mixture of sandy soil and microorganism as an inoculation source of microorganism has shown that the temperature 10days after the beginning of the experiment reached to $67.6^{\circ}C$ and lasted 16days. The experiment plot using sandy soil has shown the highest figure of $5.3{\sim}108$ CFU/g in terms of number of microorganism. The result from the experiment plots for on-site composting of fresh wood chips have shown that the experiment plot that used sandy soil with fertilizer due to supply restriction of manure as a nitrogen source in construction site reached the high temperature of $54.7^{\circ}C$ after 3days of experiment beginning and maintained heating state for 17days and reached 30.6 of C/N ratio, satisfying the standard for composted manure.

• Microbiology and Biotechnology Letters
• /
• v.18 no.6
• /
• pp.607-612
• /
• 1990

The biodegradation of Aroclor 1242 was investigated by the mixed cultivation of the natural bacterial isolates and a genetically engineered microorganism (GEM). The natural strain of MS-1003 degraded the Aroclor 1242 through the ortho-cleavage pathway, while the other strains through the meta-cleavage pathway. When the MS-1003 strain was additionally inoculated into the 1 day culture of the DJ-26 strain and then cultivated for 2 days, the Aroclor was degraded up to 86% and resulted in increase of the meta-cleavage product. But in the MS-1003 culture inoculated with the DJ-26, degradation of the Aroclor was limited to the level of each pure culture. By the mixed cultivation of the DJ-26 strain together with the DJ-12 or its GEM strain of DF-10, which degrades the Aroclor through the meta-cleavage pathway, degradation of the Aroclor as well as production of the meta-cleavage compound were lower than those of each pure culture. The degradation of Aroclor 1242 by the GEM strain was not improved over the parental strain. Therefore, a form of cometaboiism of Aroclor 1242 was found in the mixed culture of the DJ-26 and MS-1003 strains which degrade the Aroclor through the different metabolic pathway, but in the mixed culture of the DJ-26 and DJ-12 strains degrading Aroclor 1242 through the same pathway, a kind of competetion for the substrate was observed.

• Journal of Korean Society on Water Environment
• /
• v.21 no.4
• /
• pp.347-352
• /
• 2005

The isolated microorganisms, Pseudomonas stutzeri, Raoultella planticola (Klebsiella), Serratia fonticola from petroleum contaminated soil were enriched on benzene, toluene, ethylbenzene, o-xylene as carbon and energy sources, respectively. And the degradation characteristics of BTEX was observed in the mixed BTEX substrates. We found that the BTEX in mixed substrates were degraded more than 50% by three isolated microorganisms. Among three isolated microorganisms, the highest degradation rate was observed in Pseudomonas stutzeri, but the degradation rate was different according to microorganisms. In order to increase the degradation efficiency, we applied the co-culture of isolated three microorganisms. The mixture rate of pseudomonas stutzeri : Raoultella planticola (Klebsiella) : Serratia fonticola was follows ; 1:2:1, 1:1:2, and 2:1:1, respectively. In two co-culture of 1:2:1 and 1:1:2, degradation rate was lower than isolated microorganisms. However, degradation rate became higher than isolated microorganisms and the degradation rate of benzene, toluene, and ethylene was more than 95% in co-culture of 2:1:1. The degradation rate increased through the co-culture of isolated microorganisms, however, the growth rate decreased. This was resulted from the substrate competition between microorganisms. The co-culture of microorganisms is a effective method to increase the degradation efficiency of BTEX and the co-culture mixing rate is a important factor for determination of degradation efficiency.