Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
권호 표지

A Study on Isolation of BTEX Degrading Microorganism and Variation of BTEX Removal Efficiency and Microorganism Growth Rate According to Co-Culture

BTEX 분해미생물의 순수분리와 혼합 배양에 따른 기질 분해율 및 미생물 성장률 변화에 관한 연구

  • Chung, Kyung-mi (Water Environment and Remidiation Research Center, Environment & Process Technology Division, Korea Institute of Science and Technology) ;
  • Lee, Sang-hyup (Water Environment and Remidiation Research Center, Environment & Process Technology Division, Korea Institute of Science and Technology) ;
  • Lee, Han-woong (Water Environment and Remidiation Research Center, Environment & Process Technology Division, Korea Institute of Science and Technology) ;
  • Hong, Seok-won (Water Environment and Remidiation Research Center, Environment & Process Technology Division, Korea Institute of Science and Technology) ;
  • Kim, Young-o (R&D Center, Samchang Co., Ltd.) ;
  • Choi, Yong-su (Water Environment and Remidiation Research Center, Environment & Process Technology Division, Korea Institute of Science and Technology) ;
  • Yu, Myong-jin (Faculty of Environmental Engineering, The University of Seoul)
  • 정경미 (한국과학기술연구원 환경공정연구부 수질환경 및 복원연구센터) ;
  • 이상협 (한국과학기술연구원 환경공정연구부 수질환경 및 복원연구센터) ;
  • 이한웅 (한국과학기술연구원 환경공정연구부 수질환경 및 복원연구센터) ;
  • 홍석원 (한국과학기술연구원 환경공정연구부 수질환경 및 복원연구센터) ;
  • 김영오 (삼창기업(주) 부설연구소) ;
  • 최용수 (한국과학기술연구원 환경공정연구부 수질환경 및 복원연구센터) ;
  • 유명진 (서울시립대학교 도시과학대학 환경공학부)
  • Received : 2005.02.25
  • Accepted : 2005.04.11
  • Published : 2005.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

The isolated microorganisms, Pseudomonas stutzeri, Raoultella planticola (Klebsiella), Serratia fonticola from petroleum contaminated soil were enriched on benzene, toluene, ethylbenzene, o-xylene as carbon and energy sources, respectively. And the degradation characteristics of BTEX was observed in the mixed BTEX substrates. We found that the BTEX in mixed substrates were degraded more than 50% by three isolated microorganisms. Among three isolated microorganisms, the highest degradation rate was observed in Pseudomonas stutzeri, but the degradation rate was different according to microorganisms. In order to increase the degradation efficiency, we applied the co-culture of isolated three microorganisms. The mixture rate of pseudomonas stutzeri : Raoultella planticola (Klebsiella) : Serratia fonticola was follows ; 1:2:1, 1:1:2, and 2:1:1, respectively. In two co-culture of 1:2:1 and 1:1:2, degradation rate was lower than isolated microorganisms. However, degradation rate became higher than isolated microorganisms and the degradation rate of benzene, toluene, and ethylene was more than 95% in co-culture of 2:1:1. The degradation rate increased through the co-culture of isolated microorganisms, however, the growth rate decreased. This was resulted from the substrate competition between microorganisms. The co-culture of microorganisms is a effective method to increase the degradation efficiency of BTEX and the co-culture mixing rate is a important factor for determination of degradation efficiency.

Keywords

References

  1. 라현주, 장순웅, 이시진, 토양미생물을 이용한 Benzene, Toluene, Ethylbenzene 그리고 Xylene isomers(BTEX)의 분해시 기질반응, 대한환경공학회지, 22(2), pp. 375-383 (2000)
  2. 오인석, 이시진, 장순웅, A Laboratory Study on Petroleum hydrocarbons Biodegradation under Aerobic and Denitrifying Conditions, 대한환경공학회 추계학술연구발표회 논문집 (2003)
  3. 이승우, 이준명, 장덕진, Pseudomonas putida F1과 Burk holderia cepacia G4에 의한 BTEX, trichloroethylene의 분해, 한국생물공학회지, 13(5), pp. 561-568 (1998)
  4. Shim, H. J., Shin, E. B. and Yang, S. T., A Continuous Fibrous-bed Bioreactor for BTEX Biodegradation by a Co-culture of Pseudomonas putida and Pseudomonas fluorescens. Advances in Environmental Reserch, 7, pp. 203-216 (2002) https://doi.org/10.1016/S1093-0191(01)00132-0
  5. APHA, AWWA, WEF, Standard Methods for the Examination of Water and Wastewater 20th Edition, APHA, Washington D.C., USA (1998)
  6. George, Biokinetic Modeling of in Situ Bioremediation of BTX Compounds-impact of Process Variables and Scaleup Implications, water research, 37, pp. 1296-1307 (2003) https://doi.org/10.1016/S0043-1354(02)00491-8
  7. Juck, D.T., Charles, L. G. and Whyte, C. W., Polyphasic Microbial Community Analysis of Petroleum Hydrocarboncontaminated Soils from two Northern Canadian Communities, FEMS microbiology ecology, 33, pp. 241-249, (2000) https://doi.org/10.1111/j.1574-6941.2000.tb00746.x
  8. Pedro, J. J. A. and Timothy, M., Substrate Interaction of BTEX during Microbial Degradation by Pure Cultures and Mixed Culture Aquifer Slurries, American Society for Microbiology, 57(10), pp. 2981-2985 (1991)
  9. Prenafeta-Boldu, F. X., Vervoort, J. J., Grotenhuis, T. C. and Van Groenestijin, J. W., Substrate Interaction during the Biodegradation of BTEX Hydrocarbons by the Fungus Cladophialophora sp. Strain T1, Appl. Environ. Microbiol., 68(6), pp. 2660-2665 (2002) https://doi.org/10.1128/AEM.68.6.2660-2665.2002
  10. Rainer, U. M., Rolf, J. W. and Wolfgang, S., Inhibition of Anaerobic Microbial o-xylene Degradaation by Toluene in Sulfidogenic Sediment Columns and Pure Cultures, FEMS Microbiology Ecology, 47, pp. 381-386 (2004). https://doi.org/10.1016/S0168-6496(03)00303-9
  11. Shinoda, Y., Sakai, Y., Uenishi, H., Uchihashi, Y., Hiraishi, A., Yukawa, H., Yurimoto, H. and Nobuo K., Aerobic and Anaerobic Toluene Degradation by a Newly Isolated Denitrifying Bacterium, Thauera sp. Strain DNT-1, Appl. Environ. Microbiol., 70, pp. 1385-1392 (2004) https://doi.org/10.1128/AEM.70.3.1385-1392.2004
  12. Smith, R. K., The Biodegradation of Aromatic Hydrocarbons by Bacteria, Biodegradation., 1(4), pp. 191-206 (1990) https://doi.org/10.1007/BF00058836
  13. Zwolinski, M. D. Y. S. H., Schreiber, M. E., Bahr, J. M., Sewell, G. W. and Hickey, W. J., Molecular Analysis of Microbial Community Structures in Pristine and Contaminated Aquifers Field and Laboratory Microcosm Experiments, Appl. Environ. Microbiol., 65(5), pp. 2143-2150 (1999)