• The Korean Journal of Pesticide Science
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• v.16 no.1
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• pp.62-68
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• 2012

This study evaluated the screening level assessment of ecological health using four biomarkers and four bioindicators of Misgurnus anguillicaudatus as a indicator species in agricultural area of South Korea during May-June 2011. The endocrine disrupting chemical (EDC) indicators, such as vitellogenin (VTG) and gonado-somatic index (GSI), were not significantly changed in the agricultural site (p>0.05), indicating no effects. The biomarkers and bioindicators were compared between two sites of reference site (RS) and the agricultural site (AS) for screening assessment of ecological health. The ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, and DNA damage were significantly changed in the AS compared with the RS (p<0.05). But the individual level bioindicators such as condition factor (CF), hepato-somatic index (HSI), and gonado-somatic index (GSI) were not significantly different from reference site (RS). These results may indicate impairments of ecological health by toxic chemicals and environmental conditions. Current this study is based on screening assessment of biochemical and individual level biomarkers and bioindicators, so further study is required additional biomarkers and population or community level bioindicators for more specific health assessments in agricultural areas.

• Development and Reproduction
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• v.17 no.3
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• pp.187-198
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• 2013

In this study, we measured the morphometric and histological changes in the cyprinid loach, Misgurnus anguillicaudatus, during the early period of growth. Eyes, yolk length, yolk height, and yolk volume of the larva decreased for 16 days post hatching (DPH) (P<0.05). During 60 DPH (P>0.05), the most anterior extension of the head ${\times}$ the posterior end of the supraoccipital, the most anterior extension of the head ${\times}$ the origin of the dorsal fin, the most anterior extension of the head ${\times}$ the origin of the pectoral fin, the posterior end of the supraoccipital ${\times}$ the origin of the pelvic fin, and the origin of the dorsal fin ${\times}$ the ventral origin of the caudal fin gradually decreased, whereas the most anterior extension of the head ${\times}$ the dorsal origin of the caudal fin, the origin of the dorsal fin ${\times}$ the origin of the anal fin, the origin of the dorsal fin ${\times}$ the origin of the pectoral fin, and the insertion of the dorsal fin ${\times}$ the origin of the pelvic fin gradually increased (P<0.05). In the cyprinid loach, the retina is composed of six layers: the epithelial layer, ganglion cell layer, inner nuclear layer, inner plexiform layer, outer limiting membrane, and rod and cone layer (RCL). After hatching, part of the RCL gradually increased in density. The kidney and midgut epithelium were already formed in the cyprinid loach just after hatching and grew gradually in subsequent days.

• Journal of fish pathology
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• v.20 no.2
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• pp.119-127
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• 2007

Phosphoinositide-specific phospholipase Cδ (PLCδ) plays an important role in many cellular responses and is involved in the production of second messenger. The present study was conducted to characterize the catalytic and regulatory properties of the PLCδ of Misgurnus mizolepis (ML-PLCδ). The ML-PLCδ gene was cloned and expressed under according to the method of the previous report (Kim et al., 2004), and its recombinant protein was purified by successive chromatography using Ni2+-NTA affinity column. The recombinant ML-PLCδ showed a concentration-dependent PLC activity to phosphatidylinositol 4,5-bisphosphate (PIP2) or phosphatidylinositol (PI). Its activity was absolutely Ca2+-dependence, which was similar to mammalian PLCδ isozymes. The Ca2+ concentration yielding maximal activation of ML-PLCδ was 100 μM. However, the activity was decreased interestingly by a polyamine, such as spermine and spermidine. In vitro assay using cholate-micelle cell, ML-PLCδ activity was inhibited in dose-dependent manner by sphinogosine but increased by phosphocholine . In the lipid-binding assay, ML-PLCδ was strongly bound to LPA, PI(3)P, PI(4)P, PI(5)P, PI(3,5)P2, PI(4,5)P2, PI(3,4,5)P3 and PA, but it showed the low affinity to S1P, PI(3,4)P2 and PS. Taken together our results, it is suggested that the general catalytic and regulatory properties of ML-PLCδ are similar with those of mammalian PLCδ1 isozymes, but the N-terminal extended piscine phospholipase Cδ1 (ML-PLCδ) might reflect some distinctions in regulatory properties and inositol-lipid binding specificity between piscine ML-PLCδ and mammalian PLCδ isozymes.

• Korean Journal of Ichthyology
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• v.7 no.2
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• pp.187-194
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• 1995

This study was carried out to obtain fundamental data on the metabolism of hibernant fish loach, Misgurnus mizolepis. Main focus was on the compositon of muscle components and its changes in fresh - water loach before and after spawning season and before and after hibernation. Distributional changes of carbohydrate, protein and lipid in the muscle tissues were also investigated. Change patterns of miosture and crude protein, and moisture and crude lipid were in inversely proportional, i.e. : moisture amount showed the lowest value after spawnig season, the highest just after hibernation, but crude protein and crude lipid were the highest values after spawning season, and the lowest just after hibernation. Carbohydrate showed the highest value just before hibernation and tended to decrease thereafter. Muco layer of epidermis and muscle cell of hypodermis layer in loach were remarkable in its PAS dyeing degree after sapwning season, and it was presumed to include high percentage of protein or carbohydrate. Dermis layer became thinner before spawning hibernation. Lipid component in female tended to distribute relatively widely in the muscle cell layer before spawning season, but in case of male mainly in muco layer and epidermis layer. It appeared that lipid was spreaded mainly in epidermis and hypodermis tissue after spawning season, while it prevailed in almost all tissues but tended to decrease after hibernation.

• Journal of Aquaculture
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• v.19 no.3
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• pp.157-165
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• 2006

Expression of major antioxidant enzyme (AOE) including Cu/Zn superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione-S-transferase (GST) and 3 glutathione peroxidase isotypes (GPXs) at mRNA levels during heat stress was examined in mud loach (Misgurnus mizolepis) liver. Based on the semi-quantitative RT-PCR, real-time RT-PCR and/or northern dot blot hybridization, the antioxidant enzyme genes were generally up-regulated during elevation of water temperature from $23^{\circ}C$ up to $32^{\circ}C$. GPXs and SOD displayed the most significant elevation of mRNA levels (up to 3 and 2 folds, respectively) while CAT showed the steady-state expression irrespective of thermal conditions. GST represented the relatively moderate response (1.3-fold increase) in its transcription to thermal stress. The transcriptional activation of AOE genes was not significant at the treatment temperature lower than $29^{\circ}C$. Increased mRNA levels of GPX (extracellular form) and SOD genes in the fish exposed to $32^{\circ}C$ was readily detectable 1 day after exposure to heat stress.

• Korean Journal of Environmental Biology
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• v.40 no.2
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• pp.138-147
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• 2022

Paddy fields are important habitats for maintaining the biodiversity of agricultural ecosystems. Recently, as the need for safe food and biodiversity conservation has increased, the rice cultivation methods have undergone changes. This study was conducted to investigate the fish fauna in Environmentally Friendly Paddy fields (EFP) and Conventional Paddy fields (CP), and compare the characteristics of Misgurnus anguillicaudatus and Aphyocypris chinensis populations in EFP and CP. A total of 2,703 fish were collected and classified into six families and 13 species. Our results showed that there was a difference in the proportion of fish populations in the two paddy fields, and the number of individuals in EFP was higher than in CP. The weight values of M. anguillicaudatus were higher in EFP than in CP, and the total length, body length, and weight values of A. chinensis were higher in EFP than in CP. Conditional factor(K) values also showed similar trends in EFP and CP. Our results indicate that the farming practices in paddy fields have a significant effect on the characteristics of M. anguillicaudatus and A. chinensis populations, and the results of this study can be used as basic data for securing biodiversity in future agricultural ecosystems.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.361-362
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• 2003

Penicillium sp. PS-113 showed no toxicity against loach(Misgurnus mizolepis) and monnow(Oryzias latipes), a kind of biological indicator, for 96 hours at the concentrations of $1.0\;{\times}\;10^8$ conidia/ml inoculum. Additionally, it showed no toxicity against several plants such as Chinese cabbage(Brassica campestris subsp. napus var. pekinensis), mung bean(Phaseolus radiatus), etc. during their germination at the same concentration of inoculum.

• The Korean Journal of Zoology
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• v.39 no.2
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• pp.198-206
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• 1996

Adhesive membranes in the oocvtes of four loach fishes of Korea were investigated by light and electron microscopes to clarify the structural characteristics among the species. In the vitellosenic stage the adhesive membranes of oocvtes could be classified into two forms as follows: 1) fence-shaped form of Nemacheilus yoni and 2) non-adhesive membranous form of Misgurnus anguillicoudotus, M. Mizolepis, and Lefuo costuto. The adhesive membranes of oocvtes in the loach fishes showed a species specificity with reference to their habitats and spawning properties.

• Korean Journal of Ichthyology
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• v.4 no.1
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• pp.80-86
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• 1992

The ichthyofauna at the Dongchang Stream(a second tributary of the Naktong River) was surveyed from autumn to winter 1991. The fishes collected were 21 species, 18 genera belong to 7 families, and 9 species of them were found to be endemic species to Korea ; Squalidus gracilis majimae, Coreoleuciscus splendidus, Microphysogobio yaluensis, Cobitis longicorpus, Cobitis rotundicaudata, Niwaella multifasciata, Liobagrus mediadiposalis, Coreoperca herzi and Odontobutis platycephala. The dominat species were Zacco temmincki(37.3%)and Odontobutis platycephala(16.9%). and rare species were Pseudorasbora parva, Hemibarbus longirostris, Acheilognathus limbatus, Microphysogobio yaluensis, Cobitis rotundicaudata, Misgurnus mizolepis and Anguilla japonica. The indices of species diversity, eveness and dominant were 0.934, 0.707 and 0.293, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.3
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• pp.296-300
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• 1985

This study was carried out to obtain an information for the nutritional value of loach Misgurnus mizolepis, as proximate compositions, contribution of nitrogen, amino acids, three lipid classes and their fatty acid compositions. In total nitrogen contents, protein-N was 81.3% and free amino-N was 15.2%. In amino acid compositions of loach, glutamic acid, lysine, arginine and aspartic acid were the main amino acids in quantity. Total lipids of loach consisted of 57.85% of neutral lipids, 15.95% of glycolipids and 26.20.% of phospholipids. In the neutral lipids, the major fatty acids were palmitic acid, palmitoleic acid and oleic acid. The fatty acid composition in the glycolipids was similar to the pattern in tho neutral lipid, but phospholipids contained higher contents of palmitic acid, oleic acid and arachidonic acid than other lipid classes.