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Transcriptional Response of Major Antioxidant Enzyme Genes to Heat Stress in Mud Loach (Misgurnus mizolepis)  

Cho Young-Sun (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University(PKNU))
Lee Sang-Yoon (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University(PKNU))
Bang In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Kim Dong-Soo (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University(PKNU))
Nam Yoon-Kwon (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University(PKNU))
Publication Information
Journal of Aquaculture / v.19, no.3, 2006 , pp. 157-165 More about this Journal
Abstract
Expression of major antioxidant enzyme (AOE) including Cu/Zn superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione-S-transferase (GST) and 3 glutathione peroxidase isotypes (GPXs) at mRNA levels during heat stress was examined in mud loach (Misgurnus mizolepis) liver. Based on the semi-quantitative RT-PCR, real-time RT-PCR and/or northern dot blot hybridization, the antioxidant enzyme genes were generally up-regulated during elevation of water temperature from $23^{\circ}C$ up to $32^{\circ}C$. GPXs and SOD displayed the most significant elevation of mRNA levels (up to 3 and 2 folds, respectively) while CAT showed the steady-state expression irrespective of thermal conditions. GST represented the relatively moderate response (1.3-fold increase) in its transcription to thermal stress. The transcriptional activation of AOE genes was not significant at the treatment temperature lower than $29^{\circ}C$. Increased mRNA levels of GPX (extracellular form) and SOD genes in the fish exposed to $32^{\circ}C$ was readily detectable 1 day after exposure to heat stress.
Keywords
Antioxidant enzyme genes; Gene expression; Heat stress; Mud loach;
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