• Journal of the Korean Recycled Construction Resources Institute
• /
• v.11 no.2
• /
• pp.138-144
• /
• 2023

In this study, the effect of electrochemical treatment in mitigating alkali leaching into an aquatic environment was investigated. To modify the surface of cement paste, 1000 mA/m² of the direct current was passed through anodic graphite to the external mesh for 4 weeks. Then, the cement paste specimen was exposed to still water in air-tight condition to prevent natural healing of alkali leaching in the water. For 100 days of monitoring in water, the pH value was marginally increased at the electrochemical treatment, while control specimen ranked to the even higher pH accounting for 13.2 in the pH. Moreover, after the pH monitoring, the pH profile for the paste specimen indicated that the electrochemical treatment was effective in securing the higher alkalinity of cement matrix. The water obtained from alkali leaching process, was used to ecological test for Daphnia magna. It was evident that the electrochemical treatment had minimal adverse effect on ecological impact, while control specimen mostly immobilized the standard Daphnia magna.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.12 no.1
• /
• pp.75-85
• /
• 2010

The focus of this study is to evaluate the field applicability of the newly developed inorganic thixotropic-grout in various ways. In order to do this, the volume stability and the permeability of the inorganic thixotropic-grout have been measured and compared to the existing silica type grouts. In addition, the filling capability of the grout into the tail void has been evaluated through both an experiment with a miniature tail-void filling equipment and a test filling at the shield TBM construction site. The volume loss of the inorganic thixotropic-grout after a 14 day-curing under the atmosphere condition was appeared to be minimal. The excellent waterproofing ability of the inorganic thixotropic-grout was confirmed through a permeability test. The toxicity of the inorganic thixotropic-grout has been evaluated through a toxicity test of aquatic fishes (KS M 0111) and the pH value of the liquid eluviated from inorganic thixotropic-grout was measured as an average of 8.0 and a fatality rate of goldfish after 96 hours was 10% or so. The existence of harmful heavy metals in the liquid eluviated from the inorganic thixotropic-grout has been also examined through an atomic absorption spectroscopy (AAS) test. Any of harmful heavy metals were not detected and the detected level of $Cr^{6+}$ and Cd was far below the standard. Based on both an experiment with a miniature tail-void filling equipment and a test filling at the shield TBM construction site, the filling ability of inorganic thixotropic-grout into the tail void was proved to be excellent.

• Journal of Applied Biological Chemistry
• /
• v.53 no.1
• /
• pp.1-7
• /
• 2010

xylA promoter is a major promoter in xylose operon of Escherichia coli. xylA promoter is sufficient as the promoter for the construction of new expression vector because this promoter was tightly controlled and induced by the addition of xylose. For the construction of xylose-inducible expression vector, 600 bp of xylA promoter was ligated between AatII and HindIII of pUC18, named pXA600. In order to investigate the effect of XylR protein encoded by xylR gene on the xylA promoter, 1,988 bp of xylR gene including its promoter was ligated into downstream of multiple cloning site to the opposite direction of xylA promoter in pXA600, named pXAR600. For the measurement of expression level, 3,048 bp of lacZ structural gene was fused into xylA promoter in both plasmids pXA600 and pXAR600 as a reporter gene, named pXA600-lacZ and pXAR600-lacZ, respectively. The $\beta$-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E. coli JM109 was determined to be 1,641 and 2,304 unit by the induction with xylose in LB medium, respectively. The $\beta$-galactosidase activity of pXAR600-lacZ/JM109 was about 1.4 times higher by the induction with xylose than that of pXA600-lacZ/JM109. The $\beta$-galactosidase activity of pXA600-lacZ and pXAR600-lacZ in E.coli JM109 showed 6,282 and 9,320 unit by the induction with xylose in DM minimal medium, respectively. A regulator, xylR protein works as an activator for the gene expression by the addition of xylose in the xylose-inducible vectors because the level of gene expression in pXA600 is increased by the insertion of xylR gene into the same vector. The xynA gene of Streptomyces thermocyaneoviolaceus cloned in pXA600 and pXAR600 was successfully expressed in E. coli BLR(DE3). As a result, plasmids pXA600 and pXAR600 using xylA promoter are sufficient as new expression system to produce a foreign protein in E. coli.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.20 no.3
• /
• pp.593-608
• /
• 2018

If a problem occurs in the strut during the construction of the braced wall, they may cause excessive deformation of the braced wall. Therefore, in this study, the behavior of the braced wall and existing tunnel adjacent to excavation were investigated assuming that the support function of strut is lost during construction process. For this purpose, a series of model test was performed. As a result of the study, the earth pressure in the ground behind wall was rearranged due to the deformation of the braced wall, and the ground displacements caused the deformation of adjacent tunnels. When the struts located on the nearest side wall from the tunnel were removed, the deformation of the braced wall and the tunnel deformation were the largest. The magnitude of transferred earth pressure depended on the location of tunnel. The increase of the cover depth of tunnel from 0.65D to 2.65D caused the increase of the earth pressure by 25.6%. As the distance between braced wall and tunnel was increased from 0.5D to 1.0D, the transferred earth pressure increased by 16% on average. Horizontal displacements of braced wall by the removal of the strut tended to concentrate around the removed struts, and the horizontal displacement increased as the strut removal position is lowered. The tunnel displacement was maximum, when the cover depth of tunnel was 1.15D and the horizontal distance between braced wall and the side of tunnel was 0.5D. The minimal displacement occurred, when the cover depth of tunnel was 2.65D and the horizontal distance between braced wall and the side of tunnel was 1.0D. The difference between the maximum displacement and the minimum displacement was about 2 times, and the displacement was considered to be the largest when it was in the range of 1.15D to 1.65D and the horizontal distance of 0.5D.

• Journal of KIISE:Software and Applications
• /
• v.27 no.5
• /
• pp.529-542
• /
• 2000

Recent years, the use of multimedia information is rapidly increasing, and the video media is the most rising one than any others, and this field Integrates all the media into a single data stream. Though the availability of digital video is raised largely, it is very difficult for users to make the effective video access, due to its length and unstructured video format. Thus, the minimal interaction of users and the explicit definition of video structure is a key requirement in the lately developing image and video management systems. This paper defines the terms and hierarchical video structure, and presents the system, which construct the clustering-based video hierarchy, which facilitate users by browsing the summary and do a random access to the video content. Instead of using a single feature and domain-specific thresholds, we use multiple features that have complementary relationship for each other and clustering-based methods that use normalization so as to interact with users minimally. The stage of shot boundary detection extracts multiple features, performs the adaptive filtering process for each features to enhance the performance by eliminating the false factors, and does k-means clustering with two classes. The shot list of a result after the proposed procedure is represented as the video hierarchy by the intelligent unsupervised clustering technique. We experimented the static and the dynamic movie videos that represent characteristics of various video types. In the result of shot boundary detection, we had almost more than 95% good performance, and had also rood result in the video hierarchy.

• Journal of KIISE:Information Networking
• /
• v.35 no.4
• /
• pp.251-267
• /
• 2008

In this paper, we present a distributed topology control algorithm for constructing an optimized topology having a minimal search-time in unstructured peer-to-peer network. According to the proposed algorithm, each node selects the best nodes having higher hit-ratio than other nodes as many as the number being exponentially proportional to the hit-ratio of the node itself, and then it connects to them. The ensemble behavior of the proposed algorithm is very similar to the recrystrallizing phenomenon that is observed in nature. There is a partial order relationship among the hit-ratios of most nodes of constructed topology. Therefore once query message visits a node, it has a higher hit-ratio than the node that was visited last by the message. The query message even sent from freeloader can escape to the node having high hit-ratio by one hop forwarding, and it never revisits any freeloader again. Thus the search can be completed within a limited search time. We also propose the Chain-reactive search scheme using the constructed topology. Such a controlled multicasting reduces the query messages by 43 percent compared to that of the naive Gnutella using broadcasting, while it saves the search time by 94 percent. The search success rate of the proposed scheme is 99 percent.

• Strategy21
• /
• s.39
• /
• pp.140-162
• /
• 2016

The construction of Jeju Naval Base was finally completed and donated to the Republic of Korea Navy on February 26th this year. There is no doubt that the new base will contribute to the substantial augmentation of Korea's naval power and maritime security. However, we should note that the new naval base took a long and hard twenty-three years to be completed. In the 21st century, Korea should adopt a new strategy that can fulfill the security requirements of Korea for the new age of international relations. The 21st century is characterized by globalization, and in the world of globalization, a national boarder has become meaningless. In the late 20th century, after the Cold War, trade between countries have greatly increased and so did the importance of the seas. Having transformed from an agricultural country into a commercial country, Korea went from a continental state to a maritime state. Korea has become the 9th largest trading state, and obviously, the importance of the sea has become significant. Korea's national strategic focus needs to be on the sea for national survival. Thus, since the 1990s, the Korean Navy has planned to build the Jeju Naval Base. Jeju, due to its geopolitical characteristics, is extremely important to the 21st century Korea's economy and national security. Jeju is the starting point of the sea route that reaches out to the world, and at the same time, the ending point of the sea route that heads towards Korea. Jeju is located in the center of Northeast Asia and thus, Jeju Naval Base is extremely important for the area's security and order. Jeju Naval Base will be very useful not only for the maritime security of Korea, but also for keeping peace and order in Northeast Asia. Jeju Naval Base was the minimal effort against the six sea route security threats towards Korea. The six sea route threats are: 1) Threat from North Korea; 2) China's Threat towards Korea's sovereignty; 3) China's treat towards Korea's fishery; 4) Threat from Japan; 5) Threat towards Korea's sea routes; 6) Threat from recent phenomena of isolationism of the United States. Jeju Naval Base is built for both warships and civilian ships--such as cruise ships--to use. Just like the United States' Pearl Harbor, Jeju Naval Base will become not only the largest military base, but a beautiful tourist site.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.10
• /
• pp.1223-1229
• /
• 2009

To construct a new recombinant strain of Bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal Bacillus thuringiensis crystal protein, a B. thuringiensis expression vector (pHT1K-1Ac) was generated that contained the B. thuringiensis cry1Ac gene under the control of its endogenous promoter in a minimal E. coli-B. thuringiensis shuttle vector (pHT1K). This vector was introduced into a B. velezensis isolate that showed high antifungal activities against several plant diseases, including rice blast (Magnaporthe grisea), rice sheath blight (Rhizotonia solani), tomato gray mold (Botrytis cinerea), tomato late blight (Phytophthora infestans), and wheat leaf rust (Puccinia recondita), by electroporation. The recombinant B. velezensis strain was confirmed by PCR using cry1Ac-specific primers. Additionally, the recombinant strain produced a protein approximately 130 kDa in size and parasporal inclusion bodies similar to B. thuringiensis. The in vivo antifungal activity assay demonstrated that the activity of the recombinant B. velezensis strain was maintained at the same level as that of wild-type B. velezensis. Furthermore, it exhibited high insecticidal activity against a lepidopteran pest, Plutella xylostella, although its activity was lower than that of a recombinant B. thuringiensis strain, whereas wild-type B. velezensis strain did not show any insecticidal activity. These results suggest that this recombinant B. velezensis strain can be used to control harmful insect pests and fungal diseases simultaneously in one crop.

• Applied Biological Chemistry
• /
• v.20 no.3
• /
• pp.300-309
• /
• 1977

The information related to the heavy metal pollution in the environment was obtained from studies on the effects of pH, phosphate and soil properties on the adsorption of metal ions (Cd, Cu, Ni, and Zn) by soils. Three soil materials; soil 1 with low CEC (8.2 me/100g) and low organic carbon content (0.34%); soil 2 with high CEC (36.4 me/100g) and low organic carbon content (1.8%) and soil 3 with high CEC (49.9 me/100g) and high organic carbon content (14.7%) were used. Soils were adjusted to several pH's and equilibrated with metal ion mixtures of 4 different concentrations, each having equal equivalents of each metal ion (0.63, 1.88, 3.12 and 4.38 micromoles per one gram soil with and without 10 micromoles of phosphate per one gram soil). Reported here are the results of the equilibrium study on soil I. The rest of the results on soil 2 and soil 3 will be repoted subsequeutly. Generally higher metal ion concentration solution resulted in higher final metal ion concentrations in the equilibrated solution and phosphate had minimal effect except it tended to enhance removal of cadmium and zinc from equilibrated solutions while it tended to decrease the removal of copper and nickel. In soil 1, percentages of added metal ions removed at pH 5.10 were; Cu 97, Ni 69, Cd 63, and Zn 55, while increasing pH to 6.40, they were increased to Cu 90.9, Zn 99, Ni 96, and Cd 92 per As initial metal ion concentration increased, final metal ion concentrations in the equilibrated solution showed a relationship with pH of the system as they fit to the equation $p[M^{++}]=a$ pH+b where $p[M^{++}]=-log$[metal ion concentration in Mol/liter]. The magnitude of pH and soil effects were reflected in slope (a) of the equation, and were different among metal ions and soils. Slopes (a) for metal ions in the aqueous system are all 2. In soil 1 they were; Zn 1.23, Cu 0.99, Ni 0.69 and Cd 0.59 at highest concentration. The adsorption of Cd, Ni, and Zn in soil 1 could be represented by the Iangmuir isotherm. However, construction of the Iangmuir isotherm required the correction for pH differences.

• The KIPS Transactions:PartC
• /
• v.18C no.5
• /
• pp.303-316
• /
• 2011

Data aggregation is important in wireless sensor networks. However, it also introduces many security problems, one of which is that a compromised node may inject false data or drop a message during data aggregation. Most existing solutions rely on encryption, which however requires high computation and communication cost. But they can only detect the occurrence of an attack without finding the attacking node. This makes sensor nodes waste their energy in sending false data if attacks occur repeatedly. Even an existing work can identify the location of a false data injection attack but it has a limitation that at most 50% of total sensor nodes can participate in data transmission. Therefore, a novel approach is required such that it can identify an attacker and also increase the number of nodes which participate in data transmission. In this paper, we propose a monitoring-based secure data aggregation protocol to prevent against a compromised aggregator which injects false data or drops a message. The proposed protocol consists of aggregation tree construction and secure data aggregation. In secure data aggregation, we use integration of abnormal data detection with monitoring and a minimal cryptographic technique. The simulation results show the proposed protocol increases the number of participating nodes in data transmission to 95% of the total nodes. The proposed protocol also can identify the location of a compromised node which injects false data or drops a message. A communication overhead for tracing back a location of a compromised node is O(n) where n is the total number of nodes and the cost is the same or better than other existing solutions.