• Journal of Korean Neurosurgical Society
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• v.55 no.3
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• pp.131-135
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• 2014

Objective : With the growing interests of bacteria as a targeting vector for cancer treatment, diverse genetically engineered Salmonella has been reported to be capable of targeting primary or metastatic tumor regions after intravenous injection into mouse tumor models. The purpose of this study was to investigate the capability of the genetically engineered Salmonella typhimurium (S. typhimurium) to access the glioma xenograft, which was monitored in mouse brain tumor models using optical bioluminescence imaging technique. Methods : U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. After tumor formation, attenuated S. typhimurium expressing bacterial luciferase (Lux) was injected into the tail vein. Bioluminescence signals from transfected cells or bacteria were monitored using a cooled charge-coupled device camera to identify the tumor location or to trace the bacterial migration. Immunofluorescence staining was also performed in frozen sections of mouse glioma xenograft. Results : The injected S. typhimurium exclusively localized in the glioma xenograft region of U87-MG-bearing mouse. Immunofluorescence staining also demonstrated the accumulation of S. typhimurium in the brain tumors. Conclusion : The present study demonstrated that S. typhimurium can target glioma xenograft, and may provide a potentially therapeutic probe for glioma.

• Korean Journal of Animal Reproduction
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• v.12 no.2
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• pp.77-83
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• 1988

This study was done with mouse embryo to assess effects of freezing media containing sucrose, freezing metods(1-F, 0.3$^{\circ}C$/min;2-F, 3-5$^{\circ}C$/min;3-F, 15$^{\circ}C$min;4-F, LN2 vapour) and cell freezers on the embryo survival determined using the FDA test. The results are summarized as follows. 1. The FDA score obtained with 1, 2, 3 and 4-F was 3.8, 3.6, 3.2 and 3.2, respectively. There was a significant difference(P<0.05) between 1-F, 3-F and 2-F, 4-F. 2. The score at the morular stage(3.8) higher(P<0.005) than the blastocyst stage of embryos(3.2). 3. No difference (P>0.05) was found between the score obtained with a automatic embryo freezer(4.0) and a liquid nitrogen container(3.7).

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.46 no.4
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• pp.70-76
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• 2009

We proposed the device to control a computer mouse with only head movements and eye blinks so that disabilities by car or other accidents can use a computer. The mouse position were estimated from a gyro-sensor which can measure head movements, and the mouse events such as click/double click were from opto sensors which can detect the eyes flicker, respectively. The sensor was mounted on the goggle in order not to disturb the visual field. There was no difference in movement speed between ours and a general mouse, but it required 3$\sim$4 more times in the result of the experiment to evaluate spatial movements and events detection of the proposed mouse because of the low accuracy. We could eliminate cumbersome work to periodically remove the accumulated error and intuitively control the mouse using non-linear relative point method with dead zones. Optical sensors are used in the event detection circuitry designed to remove the influence of the ambient light changes, therefore it was not affected in the change of external light source.

• Biomedical Science Letters
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• v.18 no.3
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• pp.193-200
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• 2012

We examined the effects of polyamines such as putrescine and cadaverine on the biosynthesis and secretion of insulin in the mouse pancreatic ${\beta}$-cell line, MIN-6. Basal insulin secretion (BIS) and glucose-stimulated insulin secretion (GSIS) from the MIN-6 cells were significantly increased by 20 min- or 24 h-treatment with micromolar concentrations of polyamines. To determine whether the enhancement was due to increase of insulin production by polyamines, we investigated the insulin mRNA and protein production. Both insulin mRNA and protein production were found to be not significantly affected by the polyamine treatment. Next, we examined the expression of several transcription factors (TFs) related to insulin synthesis and secretion in order to identify upstream events responsible for the promotion of insulin secretion of MIN6 cells by polyamines. Of the 6 TFs tested, MafA was induced by treatment of polyamines. MafA mRNA and protein expressions increased with treatment of polyamines. Overall results suggest that cadaverine and putrescine promote the insulin secretion process rather than the insulin biosynthesis from MIN6 cells. Also MafA may be involved in the enhanced insulin secretion process. Further studies are needed to elucidate the underlying mechanisms for promotion of insulin secretion by polyamines.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.240-240
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• 1996

Staphylococcus aureus, Klebsiella pneumoniae, Enterobacter cloacae, Proteus mirabilis 및 Pseudomonas aeruginosa에 의한 mouse 복강내감염증에 대하여 DA-1131은 IPM/CS와 MEPM/CS에 비하여 현저히 우수한 치료효과를 나타내었다. 백혈구감소 mouse에서의 P. aeruginosa에 의한 복강내감염증에 대하여도 DA-1131은 IPM/CS와 MEPM 및 MEPM/CS보다 동등 이상의 우수한 치료효과를 나타내었다. K. pneumoniae를 감염균으로 한 mouse 호흡기감염 후 생존율 평가 시험에서 DA-1131 투여 mouse는 IPM/CS 및 MEPM/CS에 비하여 현저히 우수한 생존율을 나타내었고, 동시에 MEPM/CS와 유사한 폐내 생균수의 감소 pattern이 확인되었으며, IPM/CS에 비하여 균의 재증식을 억제하는 효과가 우수하였다. P. mirabilis에 의한 mouse 요로감염증에서는 DA-1131 투여군의 신장내 생균수감소는 MEPM/CS와 동등한 수준인 것으로 나타났으나 IPM/CS에 비하여는 매우 우수하였다.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.1
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• pp.63-68
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• 1994

To confirm the overcome of in vitro 2-cell block, ICR mouse I-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group(control) only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage. Developments and degenerations of ICR mouse I-cell embryos were compared in CZB medium prepared with water of three quality:(l) Milli-Q ultrafiltration water(UF);(2) Milli-Q reverse osmosis water(RO);(3) tap water(TAP). The objective was to evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryo developments were supported and the less embryos were degenerated. As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.

• Mass Spectrometry Letters
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• v.12 no.3
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• pp.112-117
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• 2021

α-Amanitin and β-amanitin are highly toxic bicyclic octapeptides responsible for the poisoning of poisonous mushrooms such as Amanita, Galerina, and Lepiota by inhibiting RNA polymerase II, DNA transcription, and protein synthesis. A sensitive, simple, and selective liquid chromatography-high resolution mass spectrometric method using parallel reaction monitoring mode was developed and validated for the simultaneous determination of α- and β-amanitin in mouse plasma to evaluate the toxicokinetics of α- and β-amanitin in mice. Protein precipitation of 5 μL mouse plasma sample with methanol as sample clean-up procedure and use of negative electrospray ionization resulted in better sensitivity and less matrix effect. The calibration curves for α- and β-amanitin in mouse plasma were linear over the range of 0.5-500 ng/mL. The intra- and inter-day coefficient of variations and accuracies for α- and β-amanitin at four quality control concentrations were 3.1-14.6% and 92.5-115.0%, respectively. The present method was successfully applied to the toxicokinetic study of α- and β-amanitin after an oral administration of α- and β-amanitin at 1.5 mg/kg dose to male ICR mice.

• Clinical and Experimental Reproductive Medicine
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• v.17 no.2
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• pp.123-128
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• 1990

Capacitation of mouse spermatozoa in vitro is brought about by epididymal secretions released into the m-Tgrode medium at the time of sperm collection. Epididymal mouse sperm suspension achived by centrifugation were preincubated for a total of 120min with aliquants being removed at 5, 30 and 120min. By gently centrifugation and resuspending in fresh medium, the fertilizing rate of unwashed 5-and 30-min suspensions was increased such that 30-min washed samples did not differ significiantly from full capacitated, highly fertile 120-min unwashed samples. When epididymal suspension was added fertilization of cumulus intact oocyte was markedly inhibited, although fertilization of zona free oocytes was unaffected. Washing sperm suspensions preincubated in the absence of $Ca^{2+}$ with the subsequent introduction of exogenous $Ca^{2+}$ resulted in a significant increase in fertilization rates over equivalent unwashed samples.

• YAKHAK HOEJI
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• v.44 no.2
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• pp.107-114
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• 2000

We have synthesized 4-isonitroso-4H-1-benzopyran and 4-amino-2,3-dihydro -4H-1-benzopyran of chromone derivatives by using condensation method. Physico-chemical properties of these compounds were measured and analyzed by UV and HPLC method. The correlation coefficient of their methanol solutions by UV were 0.9992 and 0.9994, respectively. And oxime compound was resolved within 4 min and had a detection limit of 3 ng at S/N=3 by HPLC using a reversed phase column with three solvents(MeOH, $H_2O$, HAc). The amino compound was resolved within 4.5 min and had a detection limit of 10 ng at S/N=3 by HPLC under the same conditions. Anti-diabetic effect of chromone derivatives were investigated in the streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ (45 mg/kg, i.v). The investigation of the hair growth effect of isonitrosobenzopyran and 4-aminobenzopyran on the hair of black mouse (C57BL/6) was also carried out. The administraion of their ethanol solution to the black mouse (C57BU/6) through skin them promoted the growth of hair.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.701-706
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• 2009

We proposed the device to control a computer with only a head and eye blinks so that disabilities by car accidents can use a computer. Because they have paralysis of their upper extremities such as C4~C5 paraplegics and cerebral palsy, they cannot efficiently access a general keyboard/mouse not using hands and foots. The cursor position was estimated from a gyro-sensor which can measure head movements, and the mouse event such as click/double click from opto-sensors which can detect eye blinks. The sensor was put on the proper goggle in order not to disturb the visual field. The performance of the proposed device was compared to a general optical mouse, and was used both relative and absolute coordinate in cursor positioning control. The recognition rate of click and double-click was 86% of the optical mouse, the speed of cursor movement by the proposed device was not much different from the mouse. The overall accuracy was 80%. Especially, the relative coordinate is more convenience and accuracy than the absolute coordinate, and can reduce the frequency of reset to prevent the accumulative error.