• 보건교육건강증진학회지
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• 제16권1호
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• pp.187-201
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• 1999

A purpose of this study is to provide Rousseau's health theory and health education in 『Emil』. Though Rousseau was not so much a doctor as a philosopher, he was good at health care and health education. After he had thought over healthy children for 20 years, he had written 『Emil』 for 3 years. His health theory was included in the book. He was aware of the importance of mother's milk, clothes, and physical health. Especially, he told mothers to rear a child at the breast. He understood thoroughly the demerits of medicines, and made no account of doctors and medicines. But on the other hand, He emphasized natural healing power in human body. He thought that one oneself was a physician. He thought highly of inner healing. He thought that only hygiene of medicine was useful to mankind. He prescribed that hygiene was a kind of ethics education. Therefore, his hygiene was associated with mental health and moral evaluation. Because many years have passed, today we can not accept all his assertions. But we need to stress preventive medicines, spontaneous healing, and self-care in health education.

• Journal of Community Nutrition
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• 제5권2호
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• pp.72-82
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• 2003

We here outlined our study on the variations in intake of nutrients based on four season 7 consecutive day weighed diet records (WDRs) from 80 Japanese female dietitians in 1996 - 1997. Furthermore, we reviewed Japanese, Korean and international articles investigating variability in consumption of foods as well as nutrients. The relative contributions of variation for all nutrients by person were greater than those by day, week and season. Within individual variances were greater than that between individual variances, being generally observed in Korea and in the world. The ratios of within- vs. between-individual variations ranged from 1.3-26.9 in our study, the ratios being greatest in Korean, followed by Japanese and western people. Based on within individual coefficients of variation, minimal days necessary for estimating nutrient consumption per person within 10％ (20％) of the true mean with 95％ confidence intervals were estimated. They ranged from 10-35 (3-9) days for energy and major nutrients and 15-640(4-160) days for micro-nutrients. Two Japanese studies reported that the ratios for foods were as a whole greater than those for nutrients, except for cereals, rice and milk. (J community Nutrition 5(2) : 72-82,2003).

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.87-87
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• 2002

Previously, we observed high level expression of goat β-casein/genomic hGH fusion gene in mammary gland of transgenic mice. To develop an expression vector to make a human granulocyte-colony stimulating factor (hG-CSF) protein efficiently produced in milk of transgenic animals, we designed a new bicistronic vector using the goat β-casein/genomic hGH fusion gene as regulation sequences for expression and internal ribosome entry site (IRES) as a mediator for second gene expression. This vector was constructed by insertion of encephalomyocarditis virus (EMCV) IRES-dependent second gene region coupled with hG-CSF cDNA into 3' untranslated region of an intact hGH gene. By microinjcetion, four transgenic mice were generated and three of them transmitted the bicistronic vector to their progeny. (omitted)

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.89-89
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• 2002

In this study, we examined transmission efficiency and expression level of the transgenes in the transgenic mice. The transgenic lines secreting a considerable amount of human lactoferrin(LF) thrombopoietin(TPO), interleukin-10(IL-10) into their milk were subjected to access the inheritance and maintenance of transgenic phenotype. They were bred through three generations. The transmission frequency for each generations(F9, F10, F11) of 3 lines was 38.03±10.43%(13/35), 48.33±3.76%(19/39) and 31.83±8.88%(9/28) in the LF line, 51.33±18.98%(20/38), 63.70±35.71%(12/20) and 29.57± 15.05%(8/26) in the TPO line, 38.27±17.74%(15/37), 47.47±29.88%(14/28) and 50.87±5.85%(14/28) in the IL-10 line, respectively. (omitted)

• Journal of Animal Science and Technology
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• 제58권12호
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• pp.41.1-41.7
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• 2016

Background: Over two decades of observations in the field in South East Asia and Hawai'i suggest that majority of the commercial dairy herds are of black hair coat. Hence a simple study to determine the accuracy of the observation was conducted with two large dairy herds in Hawaii in the mid-1990s. Methods: A retrospective study on longevity of Holstein cattle in the tropics was conducted using DairyComp-305 lactation information coupled with phenotypic evaluation of hair coat color in two large dairy farms. Cows were classified into 3 groups: a) black (B, >90%); b) black/white (BW, 50:50) and c) white (W, >90%). Cows with other hair coat distribution were excluded from the study. In farm A, 211 out of 970 cows were identified having 4 or more lactations. In farm B, 690 out of 1,350 cows were identified with 2 or more lactations for the study. Results: The regression analyses and the Wilcoxon-Log-rank test for survival probability showed that Holstein cattle with 90% black hair coat had greater longevity compared to Holstein cattle with 90% white hair coat. Conclusions: This study suggests that longevity of Holstein cattle in tropical regions was influenced by hair coat color and characteristics.

• Journal of Nutrition and Health
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• 제49권1호
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• pp.8-17
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• 2016

Purpose: The aim of this study is to summarize and report on the change of food allergy in elementary school students and perception and practices in school nutritionists in Korea from 1995 to 2015. Methods: The search strategy was "(food allergy AND elementary school AND Korea) AND (nutritionist OR perception OR practice)". The search was conducted via KISS, DBPIA, RISS, NDSL, PubMed, Scopus, and Google scholar and full text and abstracts on the topic of food allergy evaluating prevalence, allergen, symptom, perception and practices were included in this review. Results: Out of 1379 records found in the sources, 13 related studies were included in the final analysis. The results showed that the number of students who had experienced food allergy was increasing. The two frequent allergenic foods were eggs and milk. The perception and practices of food allergy in school nutritionists was gradually increased. Conclusion: Further objective evaluations are required to confirm the food allergy status and its management in school.

• 한국환경보건학회지
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• 제29권2호
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• pp.29-37
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• 2003

The purpose of this study was to identify the distribution of non-ortho and mono-ortho PCB congeners and homologues in adipose tissues and sera of women with breast cancer. The collected samples were 25 adipose tissues and 33 sera from women with breast cancer. The samples from the control group were 49 adipose tissues and 52 sera. The levels of three non-ortho and eight mono-ortho PCBs identified in adipose tissue and serum samples were determined by GC/MSD and GC/ECD analyses. Non-ortho and mono-ortho PCB congeners were more dominant in the control group than in the case group for serum samples. The Tetra-PCB and the Hexa-, Hepta-PCB were more dominant in tale and control groups, respectively. The level of PCB homologues in normal women was similar to that of the normal human milk samples. However, the levels of PCB homologues from breast cancer patients were almost same the level of sample from environment. As a result of this study, it is suggested that breast cancer could be related to environmental factors such as PCB level in stack gas and soil sample. More extended research should be to verify this result.

• Preventive Nutrition and Food Science
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• 제10권3호
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• pp.231-238
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• 2005

A standard $1\%$ w/v solution of CM-chitosan made from squid pen was added to milk at levels of $0.5\sim3\%$ (v/v) to improve the yield and rheological properties of cottage cheese by whey protein retention. Cheese curd did not form at levels higher than $3\%$ (v/v) CM-chitosan standard solution. Yield and total protein of cottage cheese increased up to $2\%\;by\;11\;to\;42\%\;and\;17\;to\;38\%$ respectively, compared to control cheese. Whey protein losses were decreased by 11 to $42\%$ and thus accounted for all of the increase in yield. Anomalous results were obtained at the $0.8\%$ level, which neither improved yield or whey protein retention nor stabilized rheological parameters, and at the $0.5\%$ level, which improved yield and total protein without increasing whey protein retention. Elasticity and cohesiveness of CM-chitosan-containing cheese were generally improved and stabilized during storage. Monitoring of cheese chromaticity values for four weeks revealed a delay in the onset of yellowing in cheeses with CM-chitosan compared to the controls, while the concentration of added CM-chitosan had little influence on cheese chromaticity. The addition of CM-chitosan solution could be applied directly to industrial scale cottage cheese-making without the need for any modification of the production process.

• Journal of Dairy Science and Biotechnology
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• 제28권2호
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• pp.25-30
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• 2010

For centuries, probiotics have been known to promote health and prevent diseases. In recent times, modulation of diseases related to the immune function by probiotics has been recognized as very important to the health of the host's gut. Inflammatory bowel diseases (IBDs) are the most frequently studied diseases in which probiotic administration has been tested as a potential therapy. Various in vitro and in vivo studies have been performed. The studies discussed in this review suggest several mechanisms: probiotics could modulate the gut microflora by competing with disease-causing pathogenic bacteria and could directly regulate the mucosal immune system, which activates the innate and adaptive immune systems. In addition, human clinical trials have shown alleviation of disease symptoms of ulcerative colitis (UC), Crohn's disease, etc. This study aimed to understand the molecular mechanisms underlying immune modulation by probiotics and review studies on the functional aspect of IBD alleviation by probiotics. With more scientific studies confirming the effect of probiotics, this therapy holds promise for use in alternative medicine and/or pharmaceutical preparations, given the long history of safe consumption of probiotics.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1299-1309
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• 2018

We previously reported on lactophoricin (LPcin), a cationic ${\alpha}-helical$ antimicrobial peptide derived from bovine milk, which has antimicrobial effects on Candida albicans as well as Gram-positive and Gram-negative bacteria. In this study, we designed the LPcin-YK3 peptide, a shorter analog of LPcin, and investigated its antimicrobial activity. This peptide, consisting of 15 amino acids with + 3 net charges, was an effective antimicrobial agent against the on the Gram-positive strain, Staphylococcus aureus (MIC: $0.62{\mu}g/ml$). In addition, the hemolytic activity assay revealed that the peptide was not toxic to mouse and human erythrocytes up to $40{\mu}g/ml$. We also used circular dichroism spectroscopy to confirm that peptide in the presence of lipid has ${\alpha}-helical$ structures and later provide an overview of the relationship between each structure and antimicrobial activity. This peptide is a member of a new class of antimicrobial agents that could potentially overcome the problem of bacterial resistance caused by overuse of conventional antibiotics. Therefore, it could be used as a therapeutic or natural additive, particularly in the cosmetics industry.