Fidelity of Transgene Transmission and Expression in the Transgenic Mice

  • Zheng, Z. Y. (Korea Research Institute of Bioscience and biotechnology(KRIBB)) ;
  • Y. M. Han (Korea Research Institute of Bioscience and biotechnology(KRIBB) ;
  • Y. K. Kang (Korea Research Institute of Bioscience and biotechnology(KRIBB) ;
  • K. B. Oh (Korea Research Institute of Bioscience and biotechnology(KRIBB) ;
  • W. J. Shin (Department of Animal Resources Science, Chonbuk National Universit) ;
  • Lee, K. K. (Korea Research Institute of Bioscience and biotechnology(KRIBB))
  • Published : 2002.06.01

Abstract

In this study, we examined transmission efficiency and expression level of the transgenes in the transgenic mice. The transgenic lines secreting a considerable amount of human lactoferrin(LF) thrombopoietin(TPO), interleukin-10(IL-10) into their milk were subjected to access the inheritance and maintenance of transgenic phenotype. They were bred through three generations. The transmission frequency for each generations(F9, F10, F11) of 3 lines was 38.03±10.43%(13/35), 48.33±3.76%(19/39) and 31.83±8.88%(9/28) in the LF line, 51.33±18.98%(20/38), 63.70±35.71%(12/20) and 29.57± 15.05%(8/26) in the TPO line, 38.27±17.74%(15/37), 47.47±29.88%(14/28) and 50.87±5.85%(14/28) in the IL-10 line, respectively. (omitted)

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