• 제목/요약/키워드: Mild environment

검색결과 211건 처리시간 0.029초

Characterization and sequence analysis of half of genome RNA of a new Tobamovirus (Cactus mild mottle virus) from cultivated cactus plants in Korea

  • B.E. Min;B.N. Chung;Park, J.Y.;K.H. Ryu
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.114.1-114
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    • 2003
  • A new isolate of rod-shaped virus was identified from grafted cactus, Gymnocalycium mihanovichii grafted onto Hylocereus trigonus, in Korea. The virus proved to be a new Tobamovirus and called previously as Tobamovirus-Ca for which we suggest the name Cactus mild mottle virus(CMMoV), because it produced systemic mild mosaic symptoms on its original host. CMMoV is distantly related to known species of the genus Tobamovirus on the basis of host range, serological and sequence analyses. Western blot analysis showed that CMMoV is serologically unrelated to Summons' Opuntia virus which is the only known species of the genus found in cactus plants. The 3'-terminal 2,910 nucleotides have been sequenced for the virus. The coat protein (CP) and movement protein (MP) genes encode 161 and 306 amino acids residues, respectively. The nucleotide and amino acid sequences of the CP were 39.6 % to 49.2 % and 26.4 % to 40.3 % identical to other tobamoviruses, respectively. The MP and 3' noncoding region shared 16.3 % to 23.3 % and 44.6 % to 63.4 % identities, respectively, with the members of the genus. Phylogenetic tree analysis of the CP gene revealed that CMMoV clusters with members of subgroup I of Tobamovirus. CMMoV particles contained genomic RNA along with two subgenomic RNAs, and this characteristics is common in the members of the subgroup II. This is the first information of sequence and comparative analysis of a Tobamovirus that infects cactus.

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선체의 부식쇠모강판의 대기중 및 해수중 피로강도평가에 관한 연구 (Fatigue Strength Evaluation of Rusting Decayed Hull Steel Plate in Air and in Artificial Seawater Condition)

  • 김원범;백점기;암전광정;시도호
    • 대한조선학회논문집
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    • 제43권4호
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    • pp.467-475
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    • 2006
  • Fatigue strength of hull structural steel plate, extracted from longitudinal bulkhead of a 17-year-old ore/oil carrier for renewal, was investigated in air and in artificial seawater condition. The surface of the plate was covered with corrosion pits and they proved to be crack initiation sites by fractography using SEM. From this research, it was found that the evaluation method for fatigue strength of virgin mild steel plates in air and in artificial seawater can also be applied to the evaluation of the fatigue strength of mild steel plates those were long-term exposed to a corrosive environment and their surfaces had been rusted intensively.

원형파일군에 의한 파랑제어 특성 (Effects of Wave Dissipation with Circular Cylinders)

  • 이성대;김성득
    • 한국해양공학회지
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    • 제22권4호
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    • pp.27-33
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    • 2008
  • One of the central problems in astudy of the coastal surface wave environment is predicting the transformation of waves as they propagate toward the shore. The transformation is mainly due to the existence of obstacles, such as breakwaters and vertical cylinders. In general, the types of wave transformation can be classified as follows: wave diffraction, reflection, transmission, scattering, radiation, et al. This research dealtwith wave transmission and dissipation problems for two dimensional irregular waves and vertical circular cylinders. Using the unsteady mild slope equation, a numerical model was developed to calculate the reflection and transmission of regular waves from a multiple-row circular breakwater and vertical cylinders. In addition, hydraulic model experiments were conducted with different values for the properties between tire piles and the opening ratio (distances) between the rows of the breakwater. It was found that the transmission coefficients decreased with a decrease in the opening ratio and an increase in the rows of vertical cylinders. A comparison between the results of hydraulic and numerical experiments showed reasonable agreement.

완경사 방정식을 이용한 유한요소모형에서 경사경계의 처리 (Treatment of Inclined Boundaries in a Finite Element Model for the Mild-Slope Equation)

  • 정태화;류용욱
    • 한국해안·해양공학회논문집
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    • 제24권2호
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    • pp.84-88
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    • 2012
  • 본 연구에서는 완경사 방정식을 이용한 유한요소모형에서 경사 경계면을 효율적으로 처리할 수 있는 수치기법을 소개하였다. 유한요소법은 다른 수치기법과 다르게 불규칙한 지형에도 적용이 가능하다는 장점을 가지고 있어 복잡한 지형 형태를 갖는 연안에서 발생하는 수리현상을 해석하기 위하여 빈번히 사용되었다. 그러나 유한요소모형에 사용된 지배방정식이 타원형 미분방정식인 경우에는 연직방향으로 경사진 경계면에서의 경계처리가 쉽지 않다는 단점이 있다. 본 연구에서는 Bessel 함수를 이용하여 경사진 경계면을 처리하는 방법을 소개하고 얻어진 결과를 해석해와 비교하였다.

신발 디자인 특성에 따른 정형 신발 구매 시 만족도와 구매 의도에 관한 연구 (A Study on Satisfaction and Purchasing Intentions in Purchasing Orthopedic Shoes According to Shoe Design Characteristics - For patients with mild foot disease -)

  • 박준홍;이준상
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2022년도 춘계학술대회
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    • pp.411-412
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    • 2022
  • 최근 들어 잘못된 보행 습관과 신발 착용으로 족부 질환자들이 늘어나고 있다. 신발의 발달과 생활환경으로 누구나 족부질환으로부터 안심할 수 없다. 족부질환 환자가 정형 신발 구매시 신발디자인 특성이 만족도와 구매 의도에 어떠한 영향을 미치는지 연구한다. 신발 디자인 특성은 기능성, 편의성, 심미성으로 구성하였다. 사회활동 참여 빈도, 착용 목적, 족부 질환별 상태와 특징 등 다양한 관점에서 연구된다. 연구 결과를 통해 정형 경증 족부질환 환자들이 일상생활에서 유용하게 착용할 수 있는 정형 신발 디자인 개발과 현실적인 활용과 만족으로 이어지길 기대한다.

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Pathogenicity of a Korean isolate of Pepper mild mottle virus and development of full-length cDNA clone for infectious in vitro transcripts

  • J.Y. Yoon;Park, J.K.;Y.M. Yu;K.H. Ryu
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.143.3-144
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    • 2003
  • A Korean isolate of Pepper mild mottle virus (PMMoV-Kr) was isolated from a diseased pepper crop in Chunchon, Korea. The isolate was biologically purified on Nicoticaa tabacum cv. Xanthi-nc by successive single local transfer steps, and propagated on N. tabacum cv. Samsun. PMMoV-Kr could systemically infect on N. glauca, N. benthmiana, N. occidentalis and Lycopersicon esculentum, which is typical of known isolates of PMMoV. PMMoV-Kr belongs to the pathotype P1,2 based on pepper-tobamoviral indicator experiments; Capsicn chinone harboring L3 gene revealed resistant (necrotic local lesion on inoculated leaf, HR) whereas L+, L1 and L2 pepper plants expressed susceptible reactions of mosaic systemic symptoms for the isolate. To confirm the pathology and delineate symptom determinant of the isolate, full-length cDNAs of PMMoV-Kr were amplified by RT-PCR with a primer set corresponding to the 5'- and 3'-ends of PMMoV. The RT-PCR molecules amplified from genome RNA of the isolate was cloned into the pUC18 vector. Full-length cDNA clones constructed under the control of the T7 RNA promoter could be successfully transcribed to produce in vitro transcript RNA. Infectivity of the capped transcripts and its progeny virus was verified by Western blot and RT-PCR analyses.

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진딧물 전반 딸기 바이러스 발생조사 및 딸기모틀바이러스의 계통분석 (Incidence of Aphid-Transmitted Strawberry Viruses in Korea and Phylogenetic Analysis of Korean Isolates of Strawberry Mottle Virus)

  • 권선정;윤정범;조인숙;윤주연;권태룡
    • 식물병연구
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    • 제25권4호
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    • pp.226-232
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    • 2019
  • 딸기를 감염하는 30여 종의 바이러스 중 전 세계적으로 가장 많이 발생하는 4종의 바이러스는 Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV), Strawberry vein banding virus (SVBV)로 이들은 모두 진딧물이 전반되며 경제적으로 가장 중요한 바이러스들이다. 2018-2019년까지 국내 주요 딸기 생산지에서 국내 딸기 품종을 대상으로 이들 4종 진딧물 전반 바이러스의 발생조사를 실시하였다. 그 결과, 일부 국내 딸기 품종에서 SMYEV와 SMoV가 각각 0.7%와 1.3%의 낮은 감염률로 검출되었으며 SCV와 SVBV는 전혀 검출되지 않았다. 한편, 바이러스 감염 식물에서 병징은 관찰되지 않았다. 국내에서 SMoV에 대한 염기서열은 보고된 바 없어 SMoV 국내분리주에 대한 3' untranslated region의 염기서열을 결정하고 분석하였다. 기존에 보고된 SMoV 분리주들과의 분자계통학적 유연관계 분석 결과, 대부분의 국내 분리주는 캐나다 분리주와 근연관계가 높은 것으로 나타났으며 염기서열의 진화적 측면에서 분화가 거의 일어나지 않은 분리주임을 확인하였다.

경기지역 젖소 농가 고온저감시설 활용 실태조사 및 축사내 온습도 변화 (The Survey and its Effect for Cooling Systems used in Dairy Cattle Farms in Gyeonggi)

  • 이준엽;최희철;이동현;우샘이;기광석;전중환
    • 한국축산시설환경학회지
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    • 제21권2호
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    • pp.55-64
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    • 2015
  • This survey was conducted to give the basic information and temperature and humidity variation by cooling systems in dairy farms in Korea. A total of 36 farms were surveyed in Gyeonggi province with the general information of farms such as milk production, and milk quality, types of cooling systems, and its operation method of dairy farms. All of surveyed farms have cooling fan and some have 1 more cooling systems such as sprinkler, foggy systems, ventilation duct, and shower system. Although the indoor THI of cow house was decreased by either ventilation duct or foggy system during hot season, the range belongs to mild heat stress scale for dairy cows.

Absorption and Lumiescence Spectra of Eu(Ⅲ) Complexes with Oxydiacetate and Dipicolinate in Aqueous Solution

  • 김종구;윤수경;강준길
    • Bulletin of the Korean Chemical Society
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    • 제17권9호
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    • pp.854-857
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    • 1996
  • Absorption and luminescence spectra of Eu3+ (aquo) and the two different 1: 3 Eu3+: ligand systems in aqueous solutions are measured under mild acidic pH condition. The oxydiacetate (ODA) and dipicolinate (DPA) ligands, forming the similar geometric complexes, are used in this work. The three intensity parameters, Ωλ (λ=2, 4, and 6) are empirically determined by applying the Judd-Ofelt theorem to the oscillator strengths of the six absorption bands arising from the ground 7F0 state. Among the three intensity parameters, the Ω2 is found to response markedly to a miner change in the ligand environment via the 7F0→5D0 transition. In addition, the relative oscillator strengths of the four luminescence bands in the visible region, assigned to the 5D0→7FJ (J=1, 2, 3 and 4) transitions are obtained to investigate their sensitivity to the ligand environment. Among the four bands, the 610 nm band, attributed to the 5D0→7F2 transition, shows hypersensitivity in the luminescence.

Mild stressed rat을 이용한 수종한약의 항우울효과에 대한 실험적 연구 (Anti-depressant Effect of Herbal Extracts in the Chronic Mild Stressed Rat)

  • 윤정환;신동원;배현수;신민규;홍무창
    • 동의생리병리학회지
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    • 제17권1호
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    • pp.113-117
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    • 2003
  • This study was performed to investigate the anti-depression effect of herbal extracts (Rehmanniae Radix Preparat, Corni Fructus, Lycii Fructus, Pinelliae Rhizoma, Nelumbinis Semen) in the chronic mild stressed rat. After adaptation period for 2 weeks, each herbal extract was administered to the rats divided 5 groups. For comparison, St. John's wart was administered to another group in the same environment. The chronic mild stress was induced to rats by overnight illumination for 2 days before the forced swimming test. Struggling time, first latency and rest duration were calculated during the forced swimming test to evaluate the anti-depressant effect. The results were as follows: 1. In the result of evaluation of the struggling time, there was no significance in Pinelliae Rhizoma (-1.1%), Corni Fructus (-3.9%), Rehmanniae Radix Preparat (15.2%), Lycii Fructus (4.9%), St. John's wart (25.2%). There was a significant increase only in Nelumbinis Semen (43.9%) (P=0.0037). 2. In the result of evaluation of the first latency time, there was no significance in Lycii Fructus (-21.37%), Rehmanniae Radix Preparat (38.4%), Pinelliae Rhizoma (65.5%), Corni Fructus (29.2%), St. John's wart (75.8%). There was a significant increase only in Nelumbinis Semen (90.2%) (P=0.0116). 3. In the result of evaluation of the first rest duration, there was no change in St. John's wart, and Decrease was shown in Pinelliae Rhizoma (62.4%), Corni Fructus (31.6%) Rehmanniae Radix Preparat (63.1 %), Lycii Fructus (12.4%), Nelumbinis Semen (59.0%), but there was no significance in all. 4. Nelumbinis Semen is considered to have a superior anti-depression effect than the other herbal extracts, and to have a superior and-depression effect than St. John's wart being used commonly.