• Research in Plant Disease
• /
• v.10 no.4
• /
• pp.241-247
• /
• 2004

Recently, we reported a satellite RNA (Paf-satRNA) which is encapsidated in a pepper isolate of Cucumber mosaic virus (CMV-Paf) regulated symptom attenuation of the helper virus. To characterize mild symptom domain of Paf-satRNA, a series of chimeric cDNAs of satRNAs were created by using full-length cDNA clones of Paf-satRNA and a Pep-satRNA, chlorosis-inducing satRNA in pepper plants, and analyzed for determinants of symptom attenuation. When compared the nucleotide sequences, the 3' and 5' terminal sequences of the two wild-type (wt) satRNAs contained relatively conserved sequences which are the typical to CMV satRNA. Ten bases insertions were found in PepY-satRNA, and two variable regions, 81st to 113th and 183rd to 265th from the 5'-end, were located in the middle parts of the satRNAs. To delineate the attenuated symptom-related domain for the Paf-satRNA, in vitro transcripts RNAs transcribed from the wt cDNAs and constructed chimeric cDNAs were combined with genomic RNAs, RNA1, RNA2 and RNA3, of CMV-Fny and inoculated onto Nicotiana benthamiana plants. These transcripts were fully infectious onto the N. benthamiana and infectivity was confirmed by the RT-PCR. Chimeric Paf(H/N)-satRNA and PepY(N/A)-satRNA as well as Paf-satRNA induced very mild mosaic or symptomless infection on N. benthamiana. By contrast, typical mosaic symptom and stunting of infected plants were induced when PepY-satRNA, PepY(H/N)-satRNA and Paf(N/A)-satRNA were infected to N. benthamiana. Paf-satRNA coinfected with CMV-Fny RNAs induced very mild to sympomless on pepper plants whereas PepY-satRNA-infected pepper expressed typical chlorosis mosaic symptom. Two kinds of chimeric mutants, Paf(H/N)-satRNA and PepY(N/A)-satRNA, induced mild mosaic or symptomless infection onto pepper plants, while PepY(H/N)-satRNA and Paf(N/A)-satRNA showed typical chlorosis and mosaic symptom with stunting. This results suggest that mild symptom-related domain for the Paf-satRNA was located on HpaI-NarI region.

• Corrosion Science and Technology
• /
• v.17 no.5
• /
• pp.249-256
• /
• 2018

Metallic structures in the oil and gas production undergo severe degradation due to sweet and sour corrosion caused by the presence of $CO_2$ and $H_2S$ in the fluid environment. The corrosion behavior of 304 austenitic stainless was investigated in the presence of varying concentrations of $CO_2$ or $H_2S$ and $CO_2+H_2S$ to understand the effect of the parameters either individually or in combination. Potentiodynamic polarization study revealed that a small amount of $CO_2$ aided in the formation of calcareous deposit of protective layer on passive film of 304 steel, while increase in $CO_2$ concentration ruptured the layer resulting in sweet corrosion. The presence of $S^{2-}$ damaged the passive and protective layer of the steel and higher levels increased the degradation rate. Electrochemical impedance studies revealed lower polarization resistance and impedance at higher concentration of $CO_2$ or $H_2S$, supporting the outcomes of polarization study. XRD analysis revealed different types of iron carbides and iron sulphides corresponding to sweet and sour corrosion as the corrosion products, respectively. SEM analysis revealed the presence of uniform, localized and sulphide cracking in sour corrosion and general corrosion with protective carbide layer amid for sweet corrosion.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.8 no.1
• /
• pp.23-30
• /
• 2005

A numerical scheme is proposed which is applicable to the evaluation of wave field containing floating structures, and the method is utilized to estimate the effect of the floating breakwaters to be installed in Wonjeon port near Masan. The model is based on the mild-slope equation which is widely accepted for the calculation of wave modulation near shores and an additional term is introduced to consider the wave scattering associated with the thin floating structures such as floating breakwaters. The tranquility in Wonjeon port with the floating breakwater in the east side is calculated and compared with the one with a bottom-fixed breakwater. The present method is believed to provide an efficient way of quantitative measurement of the performance of floating breakwaters.

• Smart Structures and Systems
• /
• v.19 no.3
• /
• pp.299-307
• /
• 2017

The magneto-electro-elastic (MEE) material under thermal environment exhibits pyroelectric and pyromagnetic coefficients resulting in pyroelectric and pyromagnetic effects. The pyroelectric and pyromagnetic effects on the behavior of multiphase MEE sensors bonded on top surface of a mild steel beam under thermal environment is presented in this paper. The aim of the study is to find out how samples having different volume fractions of the multiphase MEE composite behave in sensor applications. This is studied at optimal location on the beam, where the maximum electric and magnetic potentials are induced due to pyroelectric and pyromagnetic effects under clamped-free and clamped-clamped boundary conditions. The sensor which is bonded on the top surface of the beam is modeled using 8-node brick element. The MEE sensor bonded on mild steel beam is subjected to uniform temperature rise of 50K. It is assumed that beam and sensor is perfectly bonded to each other. The maximum pyroelectric and pyromagnetic effects on electric and magnetic potentials are observed when volume fraction is ${\nu}_f=0.2$. The boundary conditions significantly influence the pyroelectric and pyromagnetic effects on electric and magnetic potentials.

• Journal of People, Plants, and Environment
• /
• v.21 no.6
• /
• pp.515-521
• /
• 2018

The purpose of this study was to investigate the effects of horticultural activities with reminiscence materials and singing songs on the ability to recall words and depression in the elderly with mild dementia. A total of 20 sessions of a horticultural therapy program was held once or twice a week from July 14, 2015 to November 3, 2015 for the elderly with mind dementia at the social welfare center in D city. The subjects of this study were 13 women and the mean age was 81.2 years. The ability to recall words significantly improved from $8.6{\pm}1.8$ before the program to $9.4{\pm}0.6$ after the program (p=.012). However, there was no significant difference in the score of depression after the program. Synergistic effects were generated by applying horticultural activities, singing and reminiscence therapy activities simultaneously, and it was effective in recalling memories. As emotional functions were associated with hormones in vivo, there were emotional ups and downs at each session and short-term alternative therapies were not sufficient to completely eliminate neurotoxic substances caused by dementia.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.26 no.7
• /
• pp.1019-1025
• /
• 2022

In recent years, the number of foot disease patients due to bad walking habits and living environment is increasing. We examine how patients with mild foot disease according to shoe design characteristics affect satisfaction and purchase intention when purchasing orthopedic shoes. The design characteristics of shoes consisted of functionality, convenience, and aesthetics. It was found that aesthetics had an effect on purchasing orthopedic shoes. Even if the factors of functionality and convenience have little effect, if the aesthetic is high, purchase intention and satisfaction are affected. Various elements are required for design according to the frequency of participation in social activities for each mild foot disease and the purpose of wearing it. It is expected that the development of useful orthopedic shoe designs that patients with mild foot disease can wear in their daily life will lead to practical application and satisfaction by further supplementing the characteristic factors of orthopedic shoe design identified through the research results.

• The Plant Pathology Journal
• /
• v.37 no.6
• /
• pp.632-640
• /
• 2021

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material - chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
• /
• 2001.10a
• /
• pp.117-121
• /
• 2001

For the effective development or preservation of Tokdo, the natural environments in the ambient sea area should be well investigated. The wave deformations and wave breaking in the vicinity have much affected the bottom morphology of Tokdo as well as its ecological environment. The present study investigates the wave deformations and wave breaking through a numerical model. The final goal is to provide the fundamental wave data for the effective development or preservation of Tokdo in future. The extended mild slope equation was applied to Tokdo sea area for three different deep water wave conditions (S, SSE, NNE directions). The results showed that for the S and SSE directions the wave heights in the area between the east island and the west island were very low with the level of 1~2m, but for the NNE direction they appeared pretty high with 3~4m, In the sea area near the northwest of west island, the wave heights were low to be 1~3m for all three directions of deep water wave.

• Tribology and Lubricants
• /
• v.20 no.2
• /
• pp.91-96
• /
• 2004

Wear behavior of self-mated stainless steels in NaCl solution has been investigated. The experiment was done in the corrosive liquid of which NaCl concentration of $0\~3\%$ and temperature of $15\~90^{\circ}C$. Two kinds of wear type were observed: one is 'severe wear' type which shows gradually increasing wear volume with increasing sliding distances, the other is 'mild wear' type which shows change of wear rate from high value to low at transition distance. The specific wear rate in severe wear type was not sensitive to the liquid temperature and concentration of NaCl but stable at value of $1\times10^{-3}\;mm^3$ approximately.

• Bulletin of the Korean Chemical Society
• /
• v.35 no.3
• /
• pp.715-720
• /
• 2014

A Cu(II) complex with an three nitrogens and one sulfur coordination environment was synthesized and characterized. Its redox potential was observed at 0.483 V vs. NHE, very similar to that of a Cu-containing fungal enzyme, galactose oxidase, which catalyzes the oxidation of alcohols to corresponding aldehydes with the concomitant reduction of molecular oxygen to water. The Cu(II) complex selectively oxidizes the benzylic alcohols using TEMPO/$O_2$ under mild reaction conditions to corresponding aldehydes without forming any over-oxidation product. Moreover, the catalyst can be recovered and reused multiple times for further oxidation reactions, thus minimizing the waste generation.