• The Korean Journal of Pharmacology
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• v.29 no.1
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• pp.121-130
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• 1993

This study was done to classify the proteins involved in the specific phosphorylation using the rat peripheral blood lymphocytes (rPBL) stimulated with mitogens, phorbol 12-myristate 13-acetate (PMA) and concanavalin A (Con A). The lymphocytes were incubated with $^{32}P-orthophosphate$ before PMA or Con A stimulation. The migration patterns of the phosphorylated proteins of mitogen-treated rPBL in two dimensional electrophoretic fields were analyzed after autoradiography. The stimulation of the lymphocytes with PMA and Con A increased the phosphorylation of thirteen protein fractions. The phosphorylation intensities of the protein spots differ to the treatments of the cells with specific kinase inhibitors, H-7 and W-7. These protein fractions were grouped into 3 classes, namely, PKC-mediated, CaM kinase-mediated, and other kinase mediated proteins. The effect of the duration of the stimulation on the phosphorylated behaviors occurred concurrently, not sequentially, although each individual protein fraction had a different time for the peak phosphorylation during the stimulation period upto 30 minutes. The phosphoproteins found in the cytosolic soluble fraction were phosphorylated prior to those in the pellet, whose phosphorylations were sustained at a high level for over 10 minutes. The above results suggest that the early events in lymphocyte activation involve 3 different sets of proteins which are phosphorylated by CaM kinase, PKC and other kinases, and those kinases do not work sequentially, but rather, independently or cooperatively.

• Journal of Gastric Cancer
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• v.6 no.1
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• pp.25-30
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• 2006

Purpose: The EphB2 receptor, a member of the receptor tyrosine kinase family, is a target gene of the Wnt signaling pathway and may achieve a tumor suppressor function through regulation of cell growth and migration. Our aim was to determine whether an altered expression of EphB2 might be associated with gastric cancer development and, if so, to determine to which pathologic parameter it is linked. Materials and Methods: For the construction of the gastric cancer tissue microarray, 83 paraffin-embedded tissues containing gastric cancer areas were cored 3 times and transferred to the recipient master block. The expression patterns of EphB2 were examined on tissue microarray slides by using immunohistochemistry and were compared using pathologic parameters, including histological type, depth of invasion, lymph node metastatsis, and peritoneal dissemination. Results: The EphB2 protein was expressed in the normal gastric mucosal epithelium, especially in the bottom of the mucosa. We found loss of EphB2 expression in 30 (36.1%) of the 83 gastric cancer tissues. Statistically, loss of EphB2 expression was more common in gastric cancer with lymph-node metastasis. There was no significant correlation between EphB2 expression and depth of invasion, histologic type, or peritoneal dissemination. Conclusion: Our findings suggest that loss of EphB2 expression may represent a critical step in gastric carcinogenesis.

• Ecology and Resilient Infrastructure
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• v.1 no.2
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• pp.68-81
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• 2014

In this study, the numerical simulation regarding the process and characteristics of topography change due to the vegetation recruitment and growth was carried out by adding the vegetation growth model to two-dimensional flow and sediment transport models. The vegetation introduction and recruitment on the condition for developing an alternate bar reduced the bar migration. The vegetated area and channel width changes were more significantly influenced by changes in upstream discharge rather than the duration of low flow. When the upstream discharge decreased, the vegetation area increased and the channel width decreased. The vegetation introduction and recruitment on the condition for developing a braided channel significantly influenced the characteristics of topography changes. In the braided channel, vegetation reduced the braided index, and when the upstream discharge decreased significantly, the channel topography was changed from the braided channel to the single channel. The vegetation area decreased as the upstream discharge increased. The channel width decreased significantly after the vegetation was introduced and it also decreased as the upstream discharge decreased. It was confirmed through the numerical simulation that a decrease in flood discharge accelerated the vegetation introduction and recruitment in the channel and this allowed to confirm its influence on the characteristics of topography changes qualitatively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.464-469
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• 2013

After city of Busan has been entered to the aging society in 2000, the city has the highest aging rate among 7 representative cities in 2011. Moreover, while entire population and number of average household are decreasing, over 65 years old of elderly population is rapidly increasing. So, it is possible to enter the super-aged society, where aging rate would be about 20% after 2020. The purpose of this study is that older housing-related analysis is consisted of dong-unit, and this led microscopic analysis has become necessary. Surveys from 2000 through 2010, census aggregate (output area) unit of spatial analysis was conducted. Take advantages of this, aging population and area, soaring area, high-density areas, such as the region of interest were primary extracted, and microscopic location and spatial distribution patterns were analyzed. Upon analysis, aging population is concentrated in the city and adjacent area, the highlands, and 10 years of increasing rate was more than 30 times in certain aggregate. Regarding the characteristic of these areas, the original city center, Busan, especially concentrated and intensified in aging population. Also, 2000 to 2010, the overall distribution pattern of Busan has identified aging population that is increasingly being distributed. This is the result, which is confronted with previous research result. Entering a super aged-society for the future is accordance with migration of social costs and improve the quality of life of elderly. And this could be the basic information to use the spatial dimension for the corresponding.

• Korea journal of population studies
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• v.33 no.2
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• pp.85-111
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• 2010

South Korea has experienced a rapid fertility decline and notable mortality improvement. As the drop in TFR was quicker and greater in terms of tempo and magnitude, it cast a new challenge of population projection - how to improve the forecasting accuracy in the country with a super-low fertility pattern. This study begin with the current status of the national population projection as implemented by Statistics Korea by comparing the 2009 interim projection with the 2006 official national population projection. Secondly, this study compare the population projection system including projection agencies, projection horizons, projection intervals, the number of projection scenarios, and the number of assumptions on fertility, mortality and international migration among super-low fertility countries. Thirdly we illustrate a stochastic population projection for Korea by transforming the population rates into one parameter series. Finally we describe the future challenges of the national population projection, and propose the projection scenarios for the 2011 official population projection. To enhance the accuracy, we suggest that Statistics Korea should update population projections more frequently or distinguish them into short-term and long-term projections. Adding more than four projection scenarios including additional types of "low-variant"fertility could show a variety of future changes. We also expect Statistics Korea topay more attention to the determination of a base population that should include both national and non-national populations. Finally we hope that Statistics Korea will find a wise way to incorporate the ideas underlying the system of stochastic population projection as part of the official national population projection.

• Anatomy & Biological Anthropology
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• v.31 no.4
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• pp.105-119
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• 2018

Archaeogenetics is an academic discipline that aims to establish scientific facts of human history by integrating ancient DNA analyses with archaeological and anthropological evidence. After ancient DNA research was initiated about 30 years ago, it has been innovated so rapidly that the range of analysis has been extended toward the whole genome sequence of ancient genomes in recent 10 years. By this development, researchers have been able to study in detail the origins and migration patterns of hominin species and ancient human populations by approaches of evolutionary genetics. This study has reviewed main principles of the archaeogenetic analysis and the current trends of ancient genome studies with recent achievements. While sampling techniques and statistical analyses have been improved, typical research methods have been established by the findings on hominins and ancient western Eurasia populations. Recently, archaeogenecists have been applying the methods to studying those in other geographical areas. Nonetheless, there is still the lack of ancient genome research about populations in Eastern Asia including the Korean peninsula. This review ultimately aims to predict possibilities and promise of future ancient genome studies of ancient Korean populations.

• Korean Journal of Ornithology
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• v.25 no.2
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• pp.117-125
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• 2018

The Grey-capped greenfinch (Chloris sinica) is a polytypic species that exhibits complicated geographical variation in morphology. This study provides an intraspecific phylogeographic variability of C. sinica populations in Korea with their morphometric data. The observed morphometric variations were that Ulleung island population was morphologically distinct in bill length and depths as compared to the mainland populations. Phylogenetic relationships among mitochondrial COX1 regions provided evidence for genetic differentiation between Ulleung and mainland populations. However, their genetic distances and nucleotide diversities were very low, highlighting their recent divergence. The needs for additional research is heightened to substantiate if the genetic clines in different localities may arise in C. sinica subspecies, each of which could have different breeding and wintering habitats, distribution patterns, and migration pathways.

• Journal of Life Science
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• v.28 no.11
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• pp.1379-1385
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• 2018

Glycans are attached to proteins as in glycoproteins and proteoglycans. They are found on the exterior surface of cells. O- and N-linked glycans are very common in eukaryotic cells but may also be found in prokaryotes. The interaction of cell surface glycans with complementary glycan binding proteins located on neighboring cells, other cell types, pathogens like virus, or bacteria is crucial in biologically and biomedically important processes like pathogen recognition, cell migration, cell-cell adhesion, development, and infection. Their implication in pathological condition, suggests an important role for glycans as disease markers. In addition, a great amount of research has been shown that appropriate glycosylation of a recombinant therapeutic protein is critical for product solubility, stability, pharmacokinetics and pharmacodynamics, bioactivity, and safety. Besides, cancer-associated glycosylation changes often involve sialic acid in glycan branch which play important roles in cell-cell interaction, recognition and immunological response. This review aims at giving a comprehensive overview of the glycan's biological function and describing the relevance among the glycosylation, disease diagnosis and treatment methods. Furthermore, the high-throughput analytic methods available to measure the profile changing patterns of glycan in the blood serum as well as possible underlying biochemical mechanisms.

• Korean Journal of Heritage: History & Science
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• v.41 no.1
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• pp.99-108
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• 2008

The paleogenetic analysis has become an increasingly important subject of archaeological, anthropological, biological as well as public interest. Recently, scientific research for human skeletal remains was more activated because of increasing awareness of the valuable archaeological information by the ancient DNA analysis. State of preservation of organic remains vary in different soil and burying environmental condition. Almost all available tissue disappear to analysis ancient DNA of bone in acidic soil caused by climate and geological features in Korea. Many preserved human remains excavated in the 'Heogwakmyo'(limelayered tomb of Chosun Dynasty Period) is able to explain through the relationship between burial conditions and bone survival form the burial method and ceremony. Ancient DNA analysis of excavated human bone form ancient tomb requires to remove contaminants such as microorganism's DNA and soil components that affect authentic results. Particularly, contamination control of contemporary human DNA is major serious problem and should verified by criteria of authenticity. In order to understand migration and culture of ancient population, when possible, ancient DNA studies needs to go abreast both radiocarbon and stable isotope studies because the dietary inferences will suggest ancient subsistence and settlement patterns. Also when the paleogenetic research supported with the arts and humanities research such as physical anthropology and archaeology, more valuable ancient genetic information is providing a unique results about evolutionary and population genetics studies to reconstruct the past.

• Information Systems Review
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• v.21 no.1
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• pp.113-133
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• 2019

Gentrification, caused by residents who are being forced out due to the rise of rent with vitalization by an excessive increase of city tourists and Touristification, meaning of phenomenon of residents' migration caused by residential area turning into tourist attraction have recently pointed out as a global problem. In Korea, the phenomenon such as environmental pollution, the rise of land value and rent, and the weakening of community culture in Jeju island and Bukchon Hanok Village has appeared as well. This phenomenon has become a serious problem by hindering resident's quality of life. In this circumstance, Smart tourism city has been regarded as a solution of these problems. Hence, this study chose Busan city, which has been designated as a smart city since 2015, and examined the complex impacts of the economic benefits, social costs, environmental substantiality, cultural benefits, and technical effects derived from tourism development on the residents' deteriorated quality of life based on Fuzzy-set Qualitative Analysis (fsQCA). As a result, three patterns of the hindrance to residents' quality of life were derived. If social costs of tourism development are perceived to be large, the residents perceive deteriorated quality of life, even if they recognize essential benefits (technological and economic benefits) (Pattern #1) or secondary benefits (environmental and cultural benefits) (Pattern #2) of tourism development. In addition, some residents were found not to recognize any benefits of tourism development (Pattern #3).