• Microbiology and Biotechnology Letters
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• v.37 no.4
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• pp.355-360
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• 2009

In this study, the arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was expressed on the cell surface of S. cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The constructed plasmid, pCTAST (7.1 kb), was introduced to S. cerevisiae EBY100 cell, and yeast transformants on YPDG plate showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When S. cerevisiae EBY100/pCTAST was grown on YPDG medium, the arylsulfatase activity of cell pellet reached about 1.2 unit/mL, whereas no extracellular arylsulfatase activity was detected. The DNA ladder in agarose prepared from agar by this recombinant arylsulfatase showed similar resolution and migration patterns with a commercial agarose. This results revealed that arylsulfatase expressed on the cell surface of S. cerevisiae could be applicable to the economic production of electrophoretic-grade agarose.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.4
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• pp.541-548
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• 2013

Purpose: Marrow stromal cells (MSCs) have been known for their potential to trans-differentiate into neural and glial cells in vitro and in vivo. To investigate the influence of the developing host environment on the survival and morphological and molecular differentiation, murine MSCs transplanted into the eye of Brazilian opossum (Monodelphis domestica). Methods: Enhanced green fluorescent protein (GFP) - expressing MSCs were transplanted into developing Brazilian opossums. Animals were allowed to survive for up to 4 weeks after transplantation, at which time the eyes were prepared for immunohistochemical analysis. Results: Some transplanted MSCs survived and showed morphological differentiation into neural cells with some processes within the host vitreous chamber. Some transplanted cells expressed class III ${\beta}$-tubulin (TuJ1, a marker for neuronal cells) or glial fibrillary acid protein (GFAP, a marker for glial cells) or Nestin (a marker for neural stem cells). In addition, some transplanted cells were located in ganglion cell layer but did not show morphological and molecular differentiation. Conclusions: Our result show that the most effective stage of development for transplantation into the retina was postnatal day 16, which retinas developmentally corresponded to postnatal day 4-5 days mouse retina based on cell differentiation and lamination patterns. The present findings suggest that the age of the host appears to play a key role in determining cell fate in vivo.

• Korea journal of population studies
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• v.33 no.1
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• pp.133-160
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• 2010

This study aims to analyze marital satisfaction over the family life course and to find its determinants in Korea and Japan. The data for this study came from nationwide representative sample surveys on family in these two countries including 5,308 Korean and 4,920 Japanese men and women living with their spouses. In order to see the cultural difference and similarity in marital relations, the effects of education, income, employment, marriage gradients (normative patterns between the spouses), family stress, and quality of conjugal interactions on Korean and Japanese couples' marital satisfaction were examined. It was found that the marital satisfaction showed a U-shape pattern for both Korean and Japanese couples. In both countries husbands tend to have higher marital satisfaction than wives over the entire life course. The most important determinants of Korean and Japanese couples' marital satisfaction are good qualities of conjugal interactions including deep trust and concerns for spouse and sex life satisfaction. For Korean couples good conjugal interactions is better predictor of marital satisfaction than sex life, while for Japanese couples sex life is more important determinant of marital satisfaction.

• The korean journal of orthodontics
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• v.11 no.2
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• pp.85-100
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• 1981

Serial lateral cephalometric radiographs of 40 Korean children(25 males, 15 females) ranging in age from 6 to 13 years were studied by means of computer morphometrics and statistical analysis. As a result of this study, the following conclusions can be made: 1. In both sexes, the mean growth patterns of the face were very stable and the growth directions of the face were more prominent both forward and down-ward away from the cranial base(S-N). 2. In the mandible, the rotation and the 'wave-like' manner of migration were observed. 3. The size of the lateral faces in males was larger than females at 6 and 13 years of age, and the forms of the lateral faces in males were slightly more squares than females at 13 years of age. 4. The sex differences and practical means in the growth increments and growth rates during 7 years of the growth period were as follows: (1) In the total face, there was a significant sex difference in the growth increments(males $26.21cm^2$, females $23.24cm^2$) and growth rates(males $42.02\%$, females $39.28\%$). (2) In the facial surface 1, there was a significant sex difference in the growth increments(males $21.30cm^2$, females $19.19cm^2$) but there was no significant sex difference in the growth rates(males $41.35\%$, females $39.10\%$). (3) In the anterior total facial height(N-Ne), there was no significant sex difference in the growth increments(males 18.23mm, females 17.45mm) and the growth rates(males $18.44\%$, females $18.19\%$). (4) In the posterior total facial height(S-Go), there was a significant sex difference in the growth increments(males 14.61mm, females 12.98mm) but there was no significant sex difference in the growth rates(males $23.78\%$, females $22.25\%$). 5. The percentages of anterior upper facial height to anterior total facial height in both sexes were very stable in spite of the age increase. 6. The percentage of total facial height to standing height was reduced by the age increase, and the reduced rate of anterior total facial height(males $13.26\%$, females $13.75\%$) was larger than the posterior total facial height(males $9.95\%$, females $11.70\%$). 7. The correlations of lateral facial surfaces to the standing height and the weight were higher in males than females, but the level of correlation in males was in the moderate range.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.435-442
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• 2006

A total of 167 peculiar haplotypes confirmed from 28 cpSR variants that were observed in 19 populations of Japanese red pine in Korea through cpSSR marker analysis. Thirteen individuals that showed identical haplotype dispersed evenly in 10 populations, and the average number of effective haplotype within population was 13.37. Estimate of genetic diversity (He) was 0.987 on the basis of cpSSR haplotype variants that was equivalent to or higher than the estimates reported in other studies on some forest tree species. Estimation of genetic diversity (S.I.) on the basis of cpSSR variants composing each haplotype revealed the highest estimate of 1.109 for the population of Gangwon-Yeongwol and the lowest estimate of 0.411 for the population of Gyeongbuk Mungyeong with the average of 0.887. Most of observed cpSSR variants appeared to exist commonly in 19 populations (97.62%), and genetic differentiation of cpSSR variants among populations was turned out to be weak (${\Phi}_{ST}=0.024$). Relatively fast rate of mutation of cpSSR marker might be a major cause for such weak population differentiation. There was no identical haplotype shared between 39 population pairs of 173 pair-wise population pairs. Estimation of genetic distance among 19 populations on the basis of population pairs was also impossible, that might be resulted from restricted migration among 19 populations. Considering the observed distribution patterns of cpSSR variants in addition to the previous studies on I-SSR variants, informations on the present geographic location and genetic status of populations should be considered together for effective sustainable management of the genetic resources of Japanese red pine in Korea.

• Journal of Korean Society of Rural Planning
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• v.15 no.4
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• pp.75-87
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• 2009

This paper tries to explore the local uniqueness and evolutionary characteristics of the spatial structure of the rural dwelling houses of China's Korean Ethnic. For this purpose, the paper selected 51 typical samples in each district, analyzing their ancestral home, building age, size, construction method, family composition etc. Moreover, the paper classified the pattern of the samples and analyzed the characteristic and evolutionary process of each pattern. Conclusions based on the analysis of the ancestral homes are as follows: 1.The house pattern of these China's Korean ethnics who are originally from the Hamkyung province is mainly jeongji- access type (A-type). A-type is mainly distributed along the Tumen River and Yalu River, in the Sino-Russian borders and the inland areas of Heilongjiang province. With the migration of the China's Korean ethnics, in Sino-Russian borders and the inland areas of Heilongjiang province, A-type with the 'badang' space, vestibule-access type (C-type) and living-centered type (D-type) appeared. 2. House patterns of Korean ethnics who are originally from the Pyongan province include three types: A-type, kitchen type (B-type) and C-type. A-type is mainly distributed along the Yalu River while B-type and C-type are mainly in the inland areas of these three Northeast provinces. With the decrease of population, the merger of rooms happened in A-type; while in the B-type and C-type, bathroom and storage came to exist in the north of the room. 3. The house pattern of Korean ethnics who are originally from the Gyeongsang province is mainly B-type, which is distributed in the inland areas of Heilongjiang and Jilin provinces. Besides, C-type and D-type also exist. They are in Jilin and Liaoning provinces. In the process of evolution, storage was set in the north part of the room or the window was cancelled in order to defend the coldness in Heilongjiang area, while in Jilin and Liaoning provinces, living room came into existence, which is gradually developing to D-type.

• Economic and Environmental Geology
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• v.41 no.2
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• pp.243-252
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• 2008

The Iho and Hamdeok beaches, the major coastal beaches in Jeju Island, have been studied through size analysis and using an experimental extension pole and sediment trap in beach profile, in order to understand their textural characteristics, migration patterns, and seasonal change in beach geometry. The Iho beach is composed of coarse and medium sands, 590 m in total length. The foreshore slope is 12.3$^{\circ}$ in summer and 10.8$^{\circ}$ in winter, which shows more steeper in summer. The Hamdeok beach consisting mostly of shell fragments is 950 m long, $5.7{\sim}7.4^{\circ}$ steep and 97.4${\sim}$114.5 m wide, respectively. The suspended load drift concentrations in the studied beaches showed 4.5 mg/l during the period of summer and 33.2 mg/l in winter, and those of fine-grained sediments are derived mostly from the marine of northeastward direction. The typical beach transformation of the Iho beach is resulting from the construction of jetties in the west side that built up the sand inside the jetties, whereas the erosion is occurring on the east side of beach. The center and berm sides of the sand in the Hamdeok beach drift into the dune side during the period of the stormy winter season.

• Economic and Environmental Geology
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• v.47 no.1
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• pp.17-27
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• 2014

We investigated space-time patterns of Quaternary fault activity of the Yangsan fault zone using ESR ages in the Samnam-myeon region, Ulsan, Korea. Some of fault gouge zones consist of well-defined bands which added to the older gouge band, indicative of reactivation. During addition of new bands, the older gouge band was inactive, which represents the type I faulting mode. ESR analyses of each band of the gouge zone allow us to construct history of fault movement. The entire fault gouge zones were reactivated by type III faulting mode giving us ESR ages of the lastest reactivation. ESR dates show temporal clustering into active and inactive periods analogous to historic and paleoseismic fault activities. ESR ages and dates of fault movements indicate migration of fault activities along the Yangsan Fault Zone. Segments of the Quaternary faults in the study area are branched in the south of Sangcheon site. The earliest record of activity in segmented faults is recorded from the western segment to the northern segment. Before 750~850 ka ago, the fault gouge zone from the western segment to the northern segment were active. At 750~850 ka ago, the fault gouge zone from the eastern segment to the northern segment were active. During 630~660 ka and 480~540 ka only the northern segment was active. After 340 ka ago, the fault gouge zone from the western segment to the northern segment were active again.

• Korean Journal of Environmental Agriculture
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• v.31 no.4
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• pp.359-367
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• 2012

BACKGROUND: As natural wetlands are decreased by increment of human demand, the importance of rice fields as an alternative habitat for waterbirds is well documented. However, the relationship between waterbirds use and management practice of rice fields has not been fully understood. The present study attempted to understand the changes in temporal abundance of waterbirds and their preference for habitat types in rice fields all year round. METHODS AND RESULTS: Waterbirds census were conducted in rice fields around Asan bay in Korea during April 2009-March 2010 and April 2011-March 2012. In the bird counts, the locations of the observed birds on a 1/2,500 map were recorded along with the local habitat type (paddy, ditch, levee, road). Thirty five species of waterbirds recorded in the rice fields during the survey period and three major groups (shorebirds, herons, and waterfowls) were characterized according to season and micro-habitat use. Shorebirds visited a flooded paddy for feeding during their spring migration season (April-May), and herons used the rice field as feeding sites during their breeding periods (April-October). Most waterfowls were observed in a dry paddy to feed a fallen rice seed and stubs during the winter season (September-March). Waterbird groups selectively used micro-habitats in rice field. Shorebirds and waterfowls mainly preferred at rice paddies, while herons were attracted to most habitat types. CONCLUSION(S): Rice fields supported various waterbirds all year round and waterbird communities using the rice fields were dramatically changed according to seasonal change of rice field condition.

• The Korean Journal of Quaternary Research
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• v.5 no.1
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• pp.1-13
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• 1991

The distribution of cryophilous arctic-alpine and alpine plants in Korea is reviewed in connection with palaeoenvironment, along with a discussion to their origins, patterns of migration, and their refugia. At present, the estimated number of Korean arctic-alpine and alpine species is 419, and this includes 75 arctic-alpine species, 239 alpine species and 105 Korean endemic alpine species. The disjunctive distribution of cryophilous arctic-alpine and alpine plants is likely to be due to first, the downslope and southward expansion of those species towards the Korean peninsula as a primary refugia from the arctic region as the Pleistocene glacial phases approached, and then their subsequent isolation upslope in mountain areas toward a secondary refugia as the interglacial and post-glacial climatic ameliorations followed; secondly, the expansion of forest tree communities on lowland and montane areas subsequent to the end of the Pleistocene has had the effect of dividing formerly high mountains as a result of the increased competition; and thirdly, the general disapperance or restriction of available habitats for arctic-alpine and alpine species because of post-glacial climatic amelioration. The existence of 139 alpine species exclusively in the north of Korea may be due to the following reasons; first, frequent exchanges of alpine floras with other neighbouring East Asian regions would have been facilitated; secondly, there are numerous high mountains available for the alpine plants to survive and prosper during the post-glacial period; thirdly, the existence of easy accesses between mountains within the north, which has enabled alpine floras to migrate when necessary; and finally, the availability of diverse environments and habitats for the alpine flora of the north. However, the continued survival of those species in Korea at the world's or East Asia's southernmost limits of their distribution for many species is in danger if global warming associated with the greenhouse effect takes place.