Browse > Article
http://dx.doi.org/10.14479/jkoos.2013.18.4.541

Transplantation of Marrow Stromal Cells into the Developing Mammal Retina  

Lee, Eun-Shil (Dept. of Biology, College of Natural Sciences, Kyungpook National University)
Kwon, Oh-Ju (Dept. of Ophthalmic Optic, Busan College of Information Technology)
Ye, Eun-Ah (Neuroscience Program, Iowa State University)
Jeon, Chang-Jin (Dept. of Biology, College of Natural Sciences, Kyungpook National University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.18, no.4, 2013 , pp. 541-548 More about this Journal
Abstract
Purpose: Marrow stromal cells (MSCs) have been known for their potential to trans-differentiate into neural and glial cells in vitro and in vivo. To investigate the influence of the developing host environment on the survival and morphological and molecular differentiation, murine MSCs transplanted into the eye of Brazilian opossum (Monodelphis domestica). Methods: Enhanced green fluorescent protein (GFP) - expressing MSCs were transplanted into developing Brazilian opossums. Animals were allowed to survive for up to 4 weeks after transplantation, at which time the eyes were prepared for immunohistochemical analysis. Results: Some transplanted MSCs survived and showed morphological differentiation into neural cells with some processes within the host vitreous chamber. Some transplanted cells expressed class III ${\beta}$-tubulin (TuJ1, a marker for neuronal cells) or glial fibrillary acid protein (GFAP, a marker for glial cells) or Nestin (a marker for neural stem cells). In addition, some transplanted cells were located in ganglion cell layer but did not show morphological and molecular differentiation. Conclusions: Our result show that the most effective stage of development for transplantation into the retina was postnatal day 16, which retinas developmentally corresponded to postnatal day 4-5 days mouse retina based on cell differentiation and lamination patterns. The present findings suggest that the age of the host appears to play a key role in determining cell fate in vivo.
Keywords
Marrow stromal cells; Development; Retina; Transplantation; Differentiation; Migration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Klassen HJ, Ng TF, Kurimoto Y, Kirov I, Shatos M, Coffey P et al. Multipotent retinal progenitors express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior. Invest Ophthalmol Vis Sci. 2004;45(11):4167-4173.   DOI   ScienceOn
2 Stern JH, Temple S. Stem cells for retinal replacement therapy. Neurotherapeutics. 2011;8(4):736-743.   DOI
3 Meyer JS, Shearer RL, Capowski EE, Wright LS, Wallace KA, McMillan EL et al. Modeling early retinal development with human embryonic and induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2009;106(39):16698- 16703.   DOI   ScienceOn
4 Buchholz DE, Hikita ST, Rowland TJ, Friedrich AM, Hinman CR, Johnson LV et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. 2009;27(10):2427-2434.   DOI   ScienceOn
5 Nistor G, Seiler MJ, Yan F, Ferguson D, Keirstead HS. Three-dimensional early retinal progenitor 3D tissue constructs derived from human embryonic stem cells. J Neurosci Methods. 2010;190(1):63-70.   DOI   ScienceOn
6 Castanheira P, Torquetti L, Nehemy MB, Goes AM. Retinal incorporation and differentiation of mesenchymal stem cells intravitreally injected in the injured retina of rats. Arq Bras Oftalmol. 2008;71(5):644-650.   DOI   ScienceOn
7 Gong L, Wu Q, Song B, Lu B, Zhang Y. Differentiation of rat mesenchymal stem cells transplanted into the subretinal space of sodium iodate-injected rats. Clin Experiment Ophthalmol. 2008;36(7):666-671.   DOI   ScienceOn
8 Arnhold S, Heiduschka P, Klein H, Absenger Y, Basnaoglu S, Kreppel F et al. Adenovirally transduced bone marrow stromal cells differentiate into pigment epithelial cells and induce rescue effects in RCS rats. Invest Ophthalmol Vis Sci. 2006;47(9):4121-4129.   DOI   ScienceOn
9 Tomita M, Adachi Y, Yamada H, Takahashi K, Kiuchi K, Oyaizu H et al. Bone marrow-derived stem cells can differentiate into retinal cells in injured rat retina. Stem Cells. 2002;20(4):279-283.   DOI   ScienceOn
10 MacLaren RE, Pearson RA, MacNeil A, Douglas RH, Salt TE, Akimoto M et al. Retinal repair by transplantation of photoreceptor precursors. Nature. 2006;444(7116): 203-207.   DOI   ScienceOn
11 Sakaguchi DS, Van Hoffelen SJ, Theusch E, Parker E, Orasky J, Harper MM et al. Transplantation of neural progenitor cells into the developing retina of the Brazilian opossum: an in vivo system for studying stem/progenitor cell plasticity. Dev Neurosci. 2004;26(5-6):336-345.   DOI   ScienceOn
12 Van Hoffelen SJ, Young MJ, Shatos MA, Sakaguchi DS. Incorporation of murine brain progenitor cells into the developing mammalian retina. Invest Ophthalmol Vis Sci. 2003;44(1):426-434.   DOI   ScienceOn
13 Amato MA, Boy S, Perron M. Hedgehog signaling in vertebrate eye development: a growing puzzle. Cell Mol Life Sci. 2004;61(7-8):899-910.   DOI   ScienceOn
14 Esteve P, Bovolenta P. Secreted inducers in vertebrate eye development: more functions for old morphogens. Curr Opin Neurobiol. 2006;16(1):13-19.   DOI   ScienceOn
15 Van Raay TJ, Vetter ML. Wnt/frizzled signaling during vertebrate retinal development. Dev Neurosci. 2004;26(5-6):352- 358.   DOI   ScienceOn
16 Shatos MA, Mizumoto K, Mizumoto H, Kurimoto Y, Klassen H, Young MJ. Multipotent stem cells from the brain and retina of green mice. J Regen. Med. 2001;2(3): 13-15.
17 VandeBerg JL, Robinson ES. The Laboratory Opossum (Monodelphis Domestica) in Laboratory Research. ILAR J. 1997;38(1):4-12.   DOI
18 Palmer TD, Takahashi J, Gage FH. The adult rat hippocampus contains primordial neural stem cells. Mol Cell Neurosci. 1997;8(6):389-404.   DOI   ScienceOn
19 Sakaguchi DS, Van Hoffelen SJ, Young MJ. Differentiation and morphological integration of neural progenitor cells transplanted into the developing mammalian eye. Ann N Y Acad Sci. 2003;995:127-139.   DOI   ScienceOn
20 Sakaguchi DS, Iqbal J, Sonea I, Jacobson CD. The gray short-tailed opossum: a novel model for mammalian development. Lab animal. 1995;24(6):24-29.
21 Greenlee MH, Swanson JJ, Simon JJ, Elmquist JK, Jacobson CD, Sakaguchi DS. Postnatal development and the differential expression of presynaptic terminal-associated proteins in the developing retina of the Brazilian opossum, Monodelphis domestica. Brain Res Dev Brain Res. 1996;96(1-2):159-172.   DOI   ScienceOn
22 Greenlee MH, Roosevelt CB, Sakaguchi DS. Differential localization of SNARE complex proteins SNAP-25, syntaxin, and VAMP during development of the mammalian retina. J Comp Neurol. 2001;430(3):306-320.   DOI
23 Lee ES, Yu SH, Jang YJ, Hwang DY, Jeon CJ. Transplantation of bone marrow-derived mesenchymal stem cells into the developing mouse eye. Acta Histochem Cytochem. 2011;44(5):213-221.   DOI   ScienceOn
24 Kirsch M, Fuhrmann S, Wiese A, Hofmann HD. CNTF exerts opposite effects on in vitro development of rat and chick photoreceptors. Neuroreport. 1996;7(3):697-700.   DOI   ScienceOn
25 Yu SH, Jang YJ, Lee ES, Hwang DY, Jeon CJ. Transplantation of adipose derived stromal cells into the developing mouse eye. Acta Histochem Cytochem. 2010;43(6):123- 130.   DOI   ScienceOn
26 Fuhrmann S, Kirsch M, Hofmann HD. Ciliary neurotrophic factor promotes chick photoreceptor development in vitro. Development. 1995;121(8):2695-2706.
27 Goureau O, Rhee KD, Yang XJ. Ciliary neurotrophic factor promotes muller glia differentiation from the postnatal retinal progenitor pool. Dev Neurosci. 2004;26(5-6):359- 370.   DOI   ScienceOn
28 Xie HQ, Adler R. Green cone opsin and rhodopsin regulation by CNTF and staurosporine in cultured chick photoreceptors. Invest Ophthalmol Vis Sci. 2000;41(13):4317- 4323.
29 Takahashi M, Palmer TD, Takahashi J, Gage FH. Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina. Mol Cell Neurosci. 1998;12(6):340-348.   DOI   ScienceOn
30 Cepko CL, Austin CP, Yang X, Alexiades M, Ezzeddine D. Cell fate determination in the vertebrate retina. Proc Natl Acad Sci U S A. 1996;93(2):589-595.   DOI   ScienceOn
31 Dorrell MI, Aguilar E, Weber C, Friedlander M. Global gene expression analysis of the developing postnatal mouse retina. Invest Ophthalmol Vis Sci. 2004;45(3): 1009-1019.   DOI   ScienceOn
32 Furukawa T, Mukherjee S, Bao ZZ, Morrow EM, Cepko CL. rax, Hes1, and notch1 promote the formation of Mller glia by postnatal retinal progenitor cells. Neuron. 2000;26(2):383-394.   DOI   ScienceOn
33 Hatakeyama J, Kageyama R. Retinal cell fate determination and bHLH factors. Semin Cell Dev Biol. 2004;15(1):83- 89.   DOI   ScienceOn
34 Ramsden CM, Powner MB, Carr AJ, Smart MJ, da Cruz L, Coffey PJ. Stem cells in retinal regeneration: past, present and future. Development. 2013;140(12):2576-2585.   DOI   ScienceOn
35 Wang NK, Tosi J, Kasanuki JM, Chou CL, Kong J, Parmalee N et al. Transplantation of reprogrammed embryonic stem cells improves visual function in a mouse model for retinitis pigmentosa. Transplantation. 2010;89(8):911-919   DOI   ScienceOn
36 Streilein JW. Ocular immune privilege: therapeutic opportunities from an experiment of nature. Nat Rev Immunol. 2003;3(11):879-889.   DOI   ScienceOn