• 대한약침학회지
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• 제26권4호
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• pp.357-365
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• 2023

Objectives: Since stroke is a serious health issue, novel therapeutic strategies are required. In a mouse model of ischemic stroke, this study analyzed the potential of electroacupuncture (EA) and tenuigenin (TE) to improve the efficacy of human mesenchymal stem cell (hMSC) transplantation. Methods: Middle cerebral artery occlusion (MCAO) with reperfusion was used to generate ischemic stroke. Forty-eight male C57BL/6 mice were randomly divided into five groups: control, MCAO-operated, MCAO-EA, MCAO-TE, or MCAO + EA + TE. Subsequently, hMSCs were transplanted into the ischemic region and EA, TE, or the combination was administered. Behavior assessments and immunohistochemistry were conducted to evaluate motor and cognitive recovery and hMSCs survival, migration, and differentiation. Results: The combined treatment of EA and TE exhibited enhanced hMSCs survival, migration and differentiation into neural cell lineages while suppressing astrocyte formation. Immunohistochemistry demonstrated increased neurogenesis through hMSCs transplantation in the ischemic brain. Immediate behavioral improvements were not significantly different between groups, but there was a gradual recovery in motor and cognitive function over time. Conclusion: These findings highlight the potential of EA and TE co-treatment as a therapeutic strategy for ischemic stroke, opening avenues for further research to optimize treatment protocols and elucidate underlying mechanisms.

• 한국멀티미디어학회논문지
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• 제23권9호
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• pp.1201-1209
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• 2020

Based on the risk factor evaluation for the file format, this paper defines the procedures for presenting the long-term preservation plan for that format and the technical information registry necessary for building the system. This is a procedure to perform a risk assessment for the format, evaluate the risk, and select a long-term preservation strategy based on the information registered in the registry and information on the external signature and internal signature of the electronic record. We also reviewed the criteria for selecting appropriate long-term preservation strategies in the process and provided the criteria for adopting each detailed strategy of migration and emulation, which are long-term preservation strategies. And we implemented this process as a long-term preservation decision support system. This system can be used to provide guidelines for the maintenance, management, service and long-term preservation of information resources of electronic records in public institutions such as National Archives of Korea and Libraries.

• Journal of information and communication convergence engineering
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• 제10권1호
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• pp.61-65
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• 2012

Many public organizations have been adopting and operating various servers. These servers run on Windows, Unix, and Linux operating systems that generally use less than 10% of their capacity. For migrating a public organization to cloud computing, we must first virtualize the server environment. This article proposes a strategy for server virtualization that the National IT Industry Promotion Agency (NIPA) has done and describes the effects of a public organization migrating to cloud computing. The NIPA Computer Center planned an effective virtualization migration on various servers. This project of virtualization migration was conducted with the existing policy of separate x86 servers and Unix servers. There are three popular approaches to server virtualization: a virtual machine model, a paravirtual machine model, and virtualization at the operating system layer. We selected a VMware solution that uses the virtual machine model. We selected servers for virtualization in the following manner. Servers were chosen that had the highest rate of service usage and CPU usage and had been operating for five years or more. However, we excluded servers that require 80% or greater rates of CPU usage. After adopting the server virtualization technique, we consolidated 32 servers into 3 servers. Virtualization is a technology that can provide benefits in these areas: server consolidation and optimization, infrastructure cost reduction and improved operational flexibility, and implementation of a dual computing environment.

• Biomolecules & Therapeutics
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• 제30권6호
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• pp.616-624
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• 2022

Mebendazole (MBZ), a microtubule depolymerizing drug commonly used for the treatment of helminthic infections, has been suggested as a repositioning candidate for the treatment of brain tumors. However, the efficacy of MBZ needs further study to improve the beneficial effect on the survival of those patients. In this study, we explored a novel strategy to improve MBZ efficacy using a drug combination. When glioblastoma cells were treated with MBZ, cell proliferation was dose-dependently inhibited with an IC₅₀ of less than 1 µM. MBZ treatment also inhibited glioblastoma cell migration with an IC₅₀ of less than 3 µM in the Boyden chamber migration assay. MBZ induced G2-M cell cycle arrest in U87 and U373 cells within 24 h. Then, at 72 h of treatment, it mainly caused cell death in U87 cells with an increased sub-G1 fraction, whereas polyploidy was seen in U373 cells. However, MBZ treatment did not affect ERK1/2 activation stimulated by growth factors. The marked induction of autophagy by MBZ was observed, without any increased expression of autophagy-related genes ATG5/7 and Beclin 1. Co-treatment with MBZ and the autophagy inhibitor chloroquine (CQ) markedly enhanced the anti-proliferative effects of MBZ in the cells. Triple combination treatment with temozolomide (TMZ) (another autophagy inducer) further enhanced the anti-proliferative effect of MBZ and CQ. The combination of MBZ and CQ also showed an enhanced effect in TMZ-resistant glioblastoma cells. Therefore, we suggest that the modulation of protective autophagy could be an efficient strategy for enhancing the anti-tumor efficacy of MBZ in glioblastoma cells.

• BMB Reports
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• 제56권7호
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• pp.410-415
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• 2023

Breast cancer has become the most common cancer among women worldwide. Among breast cancers, metastatic breast cancer is associated with the highest mortality rate. Twist1, one of the epithelial-mesenchymal transition-regulating transcription factors, is known to promote the intravasation of breast cancer cells into metastatic sites. Therefore, targeting Twist1 to develop anti-cancer drugs might be a valuable strategy. In this study, LY-290181 dose-dependently inhibited migration, invasion, and multicellular tumor spheroid invasion in breast cancer cell lines. These anti-cancer effects of LY-290181 were mediated through the down-regulation of Twist1 protein levels. LY-290181 inhibited extracellular signal-regulated kinase and c-Jun N-terminal kinase signaling pathways. Therefore, our findings suggest that LY-290181 may serve as a basis for future research and development of an anti-cancer agent targeting metastatic cancers.

• 한국컴퓨터정보학회논문지
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• 제13권5호
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• pp.45-52
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• 2008

본 논문에서는 고성능 컴퓨팅 시스템의 성능 향상을 위한 효율적인 동적 작업부하 균등화 정책을 제안한다. 이 정책은 시스템 자원인 CPU와 메모리를 효율적으로 사용하여 고성능 컴퓨팅 시스템의 처리량을 최대화하고, 각 작업의 수행시간을 최소화한다. 또한 이 정책은 수행중인 작업의 메모리 요구량과 각 노드의 부하 상태를 파악하여 작업을 동적으로 할당한다. 이때 작업을 할당받은 노드가 과부하 상태가 되면 다른 노드로 작업을 이주시켜 각 노드의 작업부하를 균등하게 유지함으로써 작업의 대기시간을 줄이고, 각 작업의 수행시간을 단축한다. 본 논문에서는 시뮬레이션을 통하여 제안하는 동적 작업부하 균등화 정책이 기존의 메모리 기반의 작업부하 균등화 정책에 비해 고성능 컴퓨팅 시스템의 성능 향상 면에서 우수함을 보인다.

• Molecules and Cells
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• 제38권11호
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• pp.998-1006
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• 2015

Retinols are metabolized into retinoic acids by alcohol dehydrogenase (ADH) and retinaldehyde dehydrogenase (Raldh). However, their roles have yet to be clarified in hepatitis despite enriched retinols in hepatic stellate cells (HSCs). Therefore, we investigated the effects of retinols on Concanavalin A (Con A)-mediated hepatitis. Con A was injected into wild type (WT), Raldh1 knockout ($Raldh1^{-/-}$), $CCL2^{-/-}$ and $CCR2^{-/-}$ mice. For migration study of regulatory T cells (Tregs), we used in vivo and ex vivo adoptive transfer systems. Blockade of retinol metabolism in mice given 4-methylpyrazole, an inhibitor of ADH, and ablated Raldh1 gene manifested increased migration of Tregs, eventually protected against Con A-mediated hepatitis by decreasing interferon-${\gamma}$ in T cells. Moreover, interferon-${\gamma}$ treatment increased the expression of ADH3 and Raldh1, but it suppressed that of CCL2 and IL-6 in HSCs. However, the expression of CCL2 and IL-6 was inversely increased upon the pharmacologic or genetic ablation of ADH3 and Raldh1 in HSCs. Indeed, IL-6 treatment increased CCR2 expression of Tregs. In migration assay, ablated CCR2 in Tregs showed reduced migration to HSCs. In adoptive transfer of Tregs in vivo and ex vivo, Raldh1-deficient mice showed more increased migration of Tregs than WT mice. Furthermore, inhibited retinol metabolism increased survival rate (75%) compared with that of the controls (25%) in Con A-induced hepatitis. These results suggest that blockade of retinol metabolism protects against acute liver injury by increased Treg migration, and it may represent a novel therapeutic strategy to control T cell-mediated acute hepatitis.

• 한국전자거래학회지
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• 제10권1호
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• pp.103-120
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• 2005

본 연구의 목적은 VAN에 기반한 EDI(Electronic Data Interchange) 트랜잭션을 사용하는 기업이 ebXML(e-business using XML), 로제타넷 (RosettaNet)이나 웹 서비스와 같은 웹 기반의 개방형 전자거래 프레임워크로 전환해 나갈 수 있도록 전략적인 지침을 제공하는 것이다. EDI가 가지고 있는 다양한 한계들과 웹 기술의 발전에 힘입어 많은 기업들이 인터넷 기반의 B2B 표준들을 채택하고자 한다. 그러나 이들은 기존의 EDI 시스템을 일시에 포기하지 않으면서도 점차적으로 B2B 프레임워크로 전환할 수 있는 방법을 찾고 있다. 따라서 이러한 상황에서 EDI 기업을 위한 효과적인 전환 전략과 지침을 마련하는 것은 매우 중요한 일이다. 본 연구에서 제시된 전략적 지침은 EDI 시스템으로부터 웹 기반의 e-비즈니스 프레임워크로 전환하고자 하는 기업에게 실제적인 가이드라인으로 사용될 수 있을 것이다.

• 한국콘텐츠학회논문지
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• 제11권7호
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• pp.316-327
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• 2011

본 연구에서는 결혼이민여성이 가족생활적응을 위하여 아내, 며느리, 어머니로서 어떠한 적응 전략을 가지고 살아가고 있는지를 밝힘으로서 이들의 실천적 적응 전략을 개발하는데 유용한 함의를 도출하고자 하였다. 이를 위하여 8명의 결혼이민여성을 대상으로 현상학적 연구방법론으로 가족 내 관계의 차원 즉 부부관계, 시부모관계, 자녀관계를 중심으로 가족생활적응전략을 분석하였다. 분석결과, 부부관계 적응전략에서 '착한 남편 바라보고 살기', '남편은 나의 편이어야 함', 시부모관계 적응전략에서 '시집식구 배제하기', '돌봄의 수용', 자녀관계 적응전략에서 '자녀를 결혼생활의 목표로 삼음', '지역사회와 통합', '부모로서의 권위 갖추기', '한국 사람처럼 가르치기', 심리적 적응전략에서는 '정체성의 유지와 변화수용', '자존감 지키기', '마음비우기'의 11개 주제와, 26개의 주제묶음, 76개의 의미를 도출하였다. 이러한 결과를 토대로 결혼이민 여성의 가족관계에 따른 적응전략의 본질을 밝히고 가족생활 적응을 강화하기 위한 방안을 제시하였다.

• BMB Reports
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• 제45권11호
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• pp.623-628
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• 2012

Tissue inhibitor of metalloproteinases (TIMPs; TIMP-1, -2, -3 and -4) are endogenous inhibitor for matrix metalloproteinases (MMPs) that are responsible for remodeling the extracellular matrix (ECM) and involved in migration, invasion and metastasis of tumor cells. Unlike under normal conditions, the imbalance between MMPs and TIMPs is associated with various diseased states. Among TIMPs, TIMP-1, a 184-residue protein, is the only N-linked glycoprotein with glycosylation sites at N30 and N78. The structural analysis of the catalytic domain of human stromelysin-1 (MMP-3) and human TIMP-1 suggests new possibilities of the role of TIMP-1 glycan moieties as a tuner for the proteolytic activities by MMPs. Because the TIMP-1 glycosylation participate in the interaction, aberrant glycosylation of TIMP-1 presumably affects the interaction, thereby leading to pathogenic dysfunction in cancer cells. TIMP-1 has not only the cell proliferation activities but also anti-oncogenic properties. Cancer cells appear to utilize these bilateral aspects of TIMP-1 for cancer progression; an elevated TIMP-1 level exerts to cancer development via MMP-independent pathway during the early phase of tumor formation, whereas it is the aberrant glycosylation of TIMP-1 that overcome the high anti-proteolytic burden. The aberrant glycosylation of TIMP-1 can thus be used as staging and/or prognostic biomarker in colon cancer.