• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 하계학술발표회 논문집
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• pp.273-274
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• 2008

We present a quantitative phase microscopy for live cells. This method uses the principles of common path inteferometry and single shot phase image. This system has the ability to measure live cells quantitatively with subnanometer path length stability and millisecond scale aquisition time.

• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 하계학술발표회 논문집
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• pp.185-186
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• 2008

In this paper, the nano-grating was measured by Scanning Probe Microscopy (SPM) system using digital Proportion, Integration and Derivative (PID) control. Through this measurement, we could confirm the improvement of the vertical resolution compared with analog Proportion and Integration (PI) control method.

• Applied Microscopy
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• 제51권
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• pp.18.1-18.12
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• 2021

Hair, having distinct stages of growth, is a dynamic component of the integumentary system. Nonetheless, derangement in its structure and growth pattern often provides vital clues for the diagnosis of systemic diseases. Assessment of the hair structure by various microscopy techniques is, hence, a valuable tool for the diagnosis of several systemic and cutaneous disorders. Systemic illnesses like Comel-Netherton syndrome, Griscelli syndrome, Chediak Higashi syndrome, and Menkes disease display pathognomonic findings on hair microscopy which, consequently, provide crucial evidence for disease diagnosis. With minimal training, light microscopy of the hair can easily be performed even by clinicians and other health care providers which can, thus, serve as a useful tool for disease diagnosis at the patient's bedside. This is especially true for resource-constrained settings where access and availability of advanced investigations (like molecular diagnostics) is a major constraint. Despite its immense clinical utility and non-invasive nature, hair microscopy seems to be an underutilized diagnostic modality. Lack of awareness regarding the important findings on hair microscopy may be one of the crucial reasons for its underutilization. Herein, we, therefore, present a comprehensive overview of the available methods for hair microscopy and the pertinent findings that can be observed in various diseases.

• 한국광학회지
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• 제17권4호
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• pp.335-341
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• 2006

마이켈슨 간섭계 시스템에 두 개의 Convex Lens를 추가함으로서 평면파 참조광을 유지 시키며, 물체에 조사되는 물체광의 조사면적을 증대시킬 수 있는 변형된 마이켈슨 간섭계를 제작하였다. 변형된 마이켈슨 간섭계를 반사형 디지털 홀로그래피 현미경 시스템(Digital Holography Microscope System)에 적용한 결과 두 개의 Convex Lens를 추가하지 않은 고전 마이켈슨 간섭계를 사용한 디지털 홀로그래피 현미경 시스템 보다 넓은 영역에 대해 물체를 기록 할 수 있으며 또한 보다 넓은 영역을 재생 할 수가 있다.

• Applied Microscopy
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• 제51권
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• pp.15.1-15.2
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• 2021

Puccinia miscanthi teliospores were observed on the leaf surface of Miscanthus sinensis using a field emission scanning electron microscope. Details of teliospore mucilage could be visualized through the axial imaging of secondary electrons for a better understanding of pathogen behavior in rust diseases.

• Applied Microscopy
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• 제47권3호
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• pp.165-170
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• 2017

Gas vesicles are intracellular gas-filled protein-shelled nanocompartments. The structures are spindle or cylinder-shaped, and typically $0.1{\sim}2{\mu}m$ in length and 45~250 nm in width. A variety of prokaryotes including photosynthetic bacteria and halophilic archaea form gas vesicles in their cytoplasm. Gas vesicles provide cell buoyancy as flotation devices in aqueous habitats. They are used as nanoscale molecular reporters for ultrasound imaging for biomedical purposes. The structures in halophilic archaea are poorly resolved due to the low signal-to-noise ratio from the high salt concentration in the medium. Such a limitation can be overcome using focused ion beam-thinning or inelastically scattered electrons. As the concentric bodies (~200 nm in diameter) in fungi possess gas-filled cores, it is possible that the concept of gas vesicles could be applied to eukaryotic microbes beyond prokaryotes.

• Applied Microscopy
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• 제33권3호
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• pp.169-178
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• 2003

영상이론과 회절 이론을 같은 비중으로 다루고 이들과의 관계를 나타내는 wave optics 이론을 소개하였다. 결맞는 파동 이론과 결 안맞는 파동이론 사이의 차이를 나타내고 Abbe이론과 여러 가지 phase contrast 이론들을 small angle 근사로 접근하였다. 마지막으로 다중 성분 시스템에 대한 파동 광학의 수학적인 방법의 적용을 소개하였다.

• 세라미스트
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• 제22권4호
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• pp.368-380
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• 2019

A ceramic is used as a key material in various fields. Accordingly, the use of scanning electron microscopy is increased for the purpose of evaluating the reliability and defects of advanced ceramic materials. The scanning electron microscope is developed to overcome the limitations of optical microscopy and uses accelerated electrons for imaging. Various signals such as SE, BSE and characteristic X-rays provide useful information about the surface microstructure of specimens and, the content and distribution of chemical components. The development of electron guns, such as FEG, and the improved lens system combined with the advanced in-lens detectors and STEM-in-SEM system have expanded the applications of SEM. Automated SEM-EDS analysis also greatly increases the amount of data, enabling more statistically reliable results. In addition, X-ray CT, XRF, and WDS, which are installed in scanning electron microscope, have transformed SEM a more versatile analytical equipment. The performance and specifications of the scanning electron microscope to evaluate ceramics were reviewed and the selection criteria for SEM analysis were described.

• Applied Microscopy
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• 제26권1호
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• pp.47-57
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• 1996

This study analysed the transport properties of bladder mucosa known as the typical system of 'tight epithelia' by using TEM observation with both rapid freeze-fracture electron microscopy and thin-section method and mainly analysed the cellular characteristics of turtle bladder epithelial cells. The bladder epithelium, like other tight epithelia, consists of a heterogenous population of cells. The majority of the mucosal cells are the granular cells and may function primarily in the process of active $Na^+$ reabsorption in turtle bladder. The remaining two types of cells are rich in mitochondria and is believed to be res-ponsible for a single major transport system, namely, $H^+$ transport by A-type of cell and urinary $HCO_{3}^-$ secretion by B-type of cell. As viewed in freeze-fracture electron micrograph, the tight junctions form a continuous tight seal around the epithelial cells, thus restricting diffusion in tight epithelia. In addition, the apical surface membranes have a population of rod-shaped intramembranous particles (IMPs). It is believed that these IMPs probably represent the components of the proton pump. However, it is likely that these characteristics of the apical transporter remain to be clarified in tight epithelial cells.

• 한국전자현미경학회:학술대회논문집
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• 한국현미경학회 1999년도 제30차 추계학술대회
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• pp.70-71
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• 1999