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http://dx.doi.org/10.9729/AM.2017.47.3.165

Microscopy of Microbial Gas Vesicles  

Park, Junhyung (School of Ecology and Environmental System, Kyungpook National University)
Kim, Ki Woo (School of Ecology and Environmental System, Kyungpook National University)
Publication Information
Applied Microscopy / v.47, no.3, 2017 , pp. 165-170 More about this Journal
Abstract
Gas vesicles are intracellular gas-filled protein-shelled nanocompartments. The structures are spindle or cylinder-shaped, and typically $0.1{\sim}2{\mu}m$ in length and 45~250 nm in width. A variety of prokaryotes including photosynthetic bacteria and halophilic archaea form gas vesicles in their cytoplasm. Gas vesicles provide cell buoyancy as flotation devices in aqueous habitats. They are used as nanoscale molecular reporters for ultrasound imaging for biomedical purposes. The structures in halophilic archaea are poorly resolved due to the low signal-to-noise ratio from the high salt concentration in the medium. Such a limitation can be overcome using focused ion beam-thinning or inelastically scattered electrons. As the concentric bodies (~200 nm in diameter) in fungi possess gas-filled cores, it is possible that the concept of gas vesicles could be applied to eukaryotic microbes beyond prokaryotes.
Keywords
Buoyancy; Gas vesicle; Nanobubble; Protein shell;
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1 Bollschweiler D, Schaffer M, Lawrence C M, and Engelhardt H (2017) Cryo-electron microscopy of an extremely halophilic microbe: technical aspects. Extremophiles 21, 393-398.   DOI
2 Cherin E, Melis J M, Bourdeau R W, Yin M, Kochmann D M, Foster F S, and Shapiro M G (2017) Acoustic behavior of Halobacterium salinarum gas vesicles in the high-frequency range: experiments and modeling. Ultrasound in Med. & Biol. 43, 1016-1030.   DOI
3 Daviso E, Belenky M, Griffin R G, and Herzfeld J (2013) Gas vesicles across kingdoms: a comparative solid-state nuclear magnetic resonance study. J. Mol. Microbiol. Biotechnol. 23, 281-289.   DOI
4 Honegger R (2007) Water relations in lichens. In: Fungi in the Environment, eds. Gadd G M, Watkinson S C and Dyer P, pp. 185-200, (Cambridge University Press, Oxford).
5 Kim J H and Kim K W (2017) Electron microscopic observations of sooty moulds on crape myrtle leaves. For. Path. In press.
6 Kim K W (2009) Direct whole-mount imaging of fungal spores by energyfiltering transmission electron microscopy. Micron 40, 279-283.   DOI
7 Kim K W, Hyun J W, and Park E W (2004) Cytology of cork layer formation of citrus and limited growth of Elsinoe fawcettii in scab lesions. Eur. J. Plant Pathol. 110, 129-138.   DOI
8 Pfeifer F (2012) Distribution, formation and regulation of gas vesicles. Nat. Rev. Microbiol. 10, 705-715.   DOI
9 Pfeifer F (2015) Haloarchaea and the formation of gas vesicles. Life 5, 385-402.   DOI
10 Ramsay J P, Williamson N R, Spring D R, and Salmond G P C (2011) A quorum-sensing molecule acts as a morphogen controlling gas vesicle organelle biogenesis and adaptive flotation in an enterobacterium. Proc. Natl. Acad. Sci. 108, 14932-14937.   DOI
11 Shapiro M G, Goodwill P W, Neogy A, Yin M, Foster F S, Schaffer D V, and Conolly S M (2014) Biogenic gas nanostructures as ultrasonic molecular reporters. Nat. Nanotechnol. 9, 311-316.   DOI
12 Walsby A E (1994) Gas vesicles. Microbiol. Rev. 58, 94-144.
13 Tian J, Yang F, Cui H, Zhou Y, Ruan X, and Gu N (2015) A novel approach to making the gas-filled liposome real: based on the interaction of lipid with free nanobubble within the solution. ACS Appl. Mater. Interfaces 7, 26579-26584.   DOI