• Title/Summary/Keyword: Microscopic test

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Pulmonary Artery Aneurysm in Behcet`s Disease - Report of a Case - (Bechet씨 병에 발생한 폐동맥류: 치험 1례)

  • 김선한
    • Journal of Chest Surgery
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    • v.25 no.4
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    • pp.412-417
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    • 1992
  • Pulmonary artery aneurysm is Behcet`s disease is rare and can be fatal due to rupture. We experienced a case of pulmonary artery aneurysm in Behcet`s disease. The patient was 21 year old woman who was adimitted with three month history of dyspnea, fever and cough. On examination, she had aphthous ulcer in the mouth and erythema nodosum on the left popletial fossa and forearm, but didn`t have any lesion at eyes and genitalia. The latex fixation test for rheumatoid factor, VDRL test for syphillis, antinuclear antibody and LE cell test were all negatives. The third and fourth components of complement in the serum, serum immunoglobulin concentrations[IgG, IgM, IgA] were within normal range. The chest radiography revealed a 5x6cm sized radiopaque mass density in the left hilar region. Two months later, the mass was enlarged to 6x7cm. The IV-DSA showed a single aneurysm at the proximal part of left lower lobe artery with lingular segment artery and no distal perfusion by thombotic obstruction. The steroid therapy was done for a month, but symptoms not improved. We performed resection of lingular segment and lower lobe including the aneurysm. The microscopic findings of the operative specimen were intimal hyperplasia and fragmentation of the internal elastic fibers. She was improved without remarkable event, except infection of the operative wound.

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Electron Microscopic Observations of Mouse Liver Cell Treated with Fungal Culture Filtrates Isolated from Foodstuffs II. Results of Isolated Strains (각종 식품에서 분리한 진균 배양액으로 처리된 마우스 간세포의 전자현미경적 관찰 II. 분리균주에 대한 실험성적)

  • Deung, Young-Kun;Choi, Choon-Keun;Koh, Choon-Myung
    • Applied Microscopy
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    • v.3 no.1
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    • pp.45-54
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    • 1973
  • The present study is to determine the toxicity of the fungi isolated from foodstuffs by observing the ultrastructural changes in the mouse liver cells. The results as follows: 1. The toxin-producing fungi were screened by the methods of toxin-screening test(cyto-toxicity test against to HeLa cells and thin layer chromatography). 2. All of the experimental animals treated with isolated fungi were observed the focal necrosis and inflammatory infiltration of liver parenchymal cells. 3. It showed the cytoplasmic changes, such as dilatation of rough endoplasmic reticulum (RER), swelling of mitochondria (mi). increased number of lipid droplet (li) and glycogen (gl), detachment of ribosomes (ri) by observing the electron microscopy. 4. Nuclear and nucleolar alteration were also noted the segregation of nucleolar element and irregularity of nuclear envelopes. 5. As a mass screening, the cytotoxicity test using HeLa cells and thin layer chromatography are feasible methods to detection of the mycotoxin producing fungi from various sources.

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Water-flow Test/Performance Evaluation of Nonimpinging-type Injector used in the Hydrazine Thruster of Medium-level Thrust (중형급 하이드라진 추력기에 장착되는 비충돌형 인젝터의 수류시험 및 성능평가)

  • Jung, Hun;Kim, Jong-Hyun;Kim, Jeong-Soo;Kim, In-Tae
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.139-142
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    • 2011
  • A water-flow test for acceptance verification is carried out for a nonimpinging-type injector prior to the design-performance verification of hydrazine thruster under development. The injector used in the experiment is to be equipped on the hydrazine thruster producing 70 N of nominal thrust at an inlet pressure of 24.6 $kg_f/cm^2$. It is observed that there exist varying characteristics of atomization among the injector-nozzle orifices caused by a fabrication error which can be judged from a microscopic standpoint. On the other hand, all of the injector orifices are placed within the design criteria in an injection-angle performance.

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A Study on Spray Behavior of DME-LPG Blended Fuels in a Common-rail Injection System (커먼레일 분사 시스템에서 DME-LPG 혼합연료의 분무거동에 관한 연구)

  • Kim, W.I.;Woo, S.C.;Lee, C.S.;Lee, K.H.
    • Journal of ILASS-Korea
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    • v.20 no.1
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    • pp.35-42
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    • 2015
  • This study is to investigate the spray behavior of DME-LPG blended fuels in common rail injection system for diesel engines. The visualization experiment was performed to analyze the macroscopic spray behavior of test fuels. In addition, the experiment using BOS(Background Oriented Schlieren) method is performed to compare liquid phase and gas phase. The test fuels are injected in high pressure chamber. The ambient pressure of high pressure chamber was formed by nitrogen gas. Spray tip penetration, spray cone angle and spray area were measured using high speed camera. SMD(Sauter Mean Diameter) and spray particle velocity were measured using the PDPA(Phase Doppler Particle Analyzer) system to analyze the microscopic properties of test fuels. The results of this experiment showed that spray tip penetration, spray cone angle and spray area of DME-LPG fuels are similar to those of DME fuel. When compared to results of experiment using BOS, significant differences of spray tip penetrations, spray cone angle and spray area are showed because of gas phase. The results of experiment using BOS method showed higher values. SMD of DME-LPG blended fuels is smaller than that of DME fuel. Velocity of DME-LPG blended fuels is faster than that of DME fuel.

An Experimental Study on the Leakage Characteristics and Durability Evaluation of an LPLi Injector (LPLi 인젝터의 누설특성 및 내구평가에 관한 실험적 연구)

  • Choi, Young;Kim, Chang-Up;Oh, Seung-Mook;Kang, Kern-Yong
    • Journal of ILASS-Korea
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    • v.12 no.4
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    • pp.204-210
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    • 2007
  • The worldwide energy problem and global warming cause the need of alternative fuels which feature low carbon-dioxide emission and another energy source. Liquefied Petroleum Gas (LPG) is one of the alternative fuels widely used as domestic and transportational fuel. The third generation LPLi fuel supply system has merits in the increase of engine power and low emissions. The injectors used in LPLi system should overcome a leakage problem and satisfy the durability conditions. Therefore, 1000 hour durability test of the injectors was carried out throughout this research. First, the spray pattern and the penetration length of the selected injectors is graphically shown. Next, the leakage amount with respect to the injection cycle is introduced. Finally, the shapes of nozzle holder and nozzle tip after durability test was investigated by analyzing the microscopic image of the injector tip. The variation in the shape of nozzle tip mainly due to the residue of rubber materials is found to be the reason for leakage.

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Effect of heat treatment on mechanical properties of overlay welds (육성 용접부의 기계적 성질에 미치는 열처리조건의 영향)

  • 이기호;김기철;윤의박
    • Journal of Welding and Joining
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    • v.7 no.4
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    • pp.30-37
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    • 1989
  • Effect of heat treatment on mechanical properties of an overlay weldment was investigated. Over welding was carried out on the structural C-Mn mild steel substrate to take required test specimens. Shielded metal arc welding process with 13Cr-0.2Ni stick electrode was applied. The heat treatment temperatures and holding times were $450{\circ}C., 550{\circ}C., 650{\circ}C., 750{\circ}C., 850{\circ}C.$ and 0.5hr, 2hr, 10hr, respectively. Mechanical tests and microscopic inspection were also carried out to investigate welds soundness. Test results indicated that carbon migration was dominant near bonded zone. At temperature of around 650.deg. C, carburized layer and decarburized layer were formed remarkably along overlay welds region and C-Mn mild steel region, respectively. The wideth of these layers became wider with increasing heat treatment temperature and/or holding time at the elevated temperature, and this relationship agreed with Larson-Miller parameter. Side bending test results demonstrated that the crack free region of overlay welds could be deduced from the relationship between temperature and holding time.

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Fracture Strength Measurement of Single Crystal Silicon Chips as a Function of Loading Rate during 3-Point Bending Test (3점 굴곡 실험에서 하중 속도 변화에 따른 단결정 실리콘 칩의 파괴강도 측정)

  • Lee, Dong-Ki;Lee, Seong-Min
    • Korean Journal of Metals and Materials
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    • v.50 no.2
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    • pp.146-151
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    • 2012
  • The present article shows how the fracture strength of single crystal silicon chips, which are generally used as semiconductor devices, is influenced by loading rate variation during a 3-point bending test. It was found that the fracture strength of the silicon chips slightly increases up to 4% with increasing loading rate for loading rates lower than 20 mm/min. Meanwhile, the fracture strength of the chips hardly increases with increase of loading rate to levels higher than 40 mm/min. However, there was an abrupt transition in the fracture strength within a loading rate range of 20 mm/min to 40 mm/min. This work explains through microscopic examination of the fracture surface of all test chips that such a big transition is related to the deflection of crack propagation direction from the (011) [${\bar{1}}00$] system to the (111) [${\bar{2}}11$] system in a particular loading rate (i.e. from 20 mm/min to 40 mm/min).

Effects of Citrate-capped Silver Nanoparticles on the Blood Coagulation and Platelet Aggregation in Rats (랫드의 혈액응고 및 혈소판 응집에 미치는 은나노 입자의 영향)

  • Lee, Yeonjin;Park, Kwangsik
    • YAKHAK HOEJI
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    • v.56 no.6
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    • pp.382-389
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    • 2012
  • Effects of citrate-capped silver nanoparticles (AgNPs) on the blood coagulation and platelet aggregation were investigated using whole blood, platelet rich plasma (PRP) and washed platelet obtained from SD male rats. To confirm the stability of AgNPs in the test, size distribution of the nanoparticles was measured in the vehicles including distilled water, serum, and platelet buffers. The average size of AgNPs was 20 nm in the vehicles, which means that the stability was maintained during the whole experimental period. When blood coagulation was monitored by using whole blood impedance aggregometer, coagulation was not observed at the concentration of 1, 10 and 50 ppm. Platelets in plasma or in buffer were not aggregated by AgNPs at the concentration of 1, 2 and 4 ppm, respectively. The test concentration of AgNPs could not be increased because the dark color of the nanoparticles impeded the transmission of light, which is an indicator of aggregation. Although the blood or platelets were pre-activated by collagen, thrombin, or ADP with sub-threshold level, aggregation was not observed at the test concentration. Microscopic observation also supported the result obtained by the aggregometer.

A Study of Material Characteristics of 120mm-Thick SM490TMC Plate (SM490TMC 극후판재 120mm의 소재특성에 관한 연구)

  • Kim, Sang Seup;Lee, Cheol Ho;Lee, Eun Taik;Han, Tae Ho;Choi, Young Han;Kim, Jong Rak
    • Journal of Korean Society of Steel Construction
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    • v.28 no.5
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    • pp.313-323
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    • 2016
  • Seven types of tests were conducted to experimentally evaluate the material properties of ultral-thick (or 120mm-thick) SM490TMC plate. The investigation of through-thickness properties was among the most significant considerations. All chemical and mechanical test results showed the through-thickness homogeneity as required and conformed to the KS(Korean industrial Standards), although the thickness was 1.5 times thicker than the thickness limit (80mm) imposed by Steel Structure Design Code. No reduction in the yield strength of 120mm-thick SM490TMC plate is recommended for design.

28-day Repeated-dose Toxicity Study of Flutamide, an Anti- androgenic Agent, in Rats: Establishment of Screening Methods for Endocrine Disruptors (내분비계 장애물질 검색법의 확립을 위한 항안드로젠성 물질 flutamide의 랫드 28일 반복투여 독성실험)

  • 정문구;김종춘;임광현;하창수
    • Toxicological Research
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    • v.16 no.2
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    • pp.163-172
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    • 2000
  • Recently, there is a worldwide concern that a great number of man-made chemicals have a hormone-like action both in humans and in animals. DECD is developing screening programs using validated test systems to determine whether certain substances may have an effect in humans. In the present study. the establishment oj repeated-dose toxicity test method was tried. Flutamide. an anti-androgenic agent. was administered by gavage to Sprague-Dawley rats for 28 days at dose levels of 0. 0.5. 3 and 18 mg/kg body weight (10-15 rats/sex/group) to examine the effects on general findings. especially reproductive and endocrine parameters. Clinical signs. body weights, food consumption, and sexual cycle were checked and measured. For the gross and microscopic examinations. 10 rats/sex/group were sacrificed at the end of dosing period and the remaining animals of control and high dose groups (5 each) were sacrificed after 14 days recovery. Examinations for hematology and clinical chemistry were carried out at necropsy. There were no treatment-related changes in clinical signs. body weights, food consumption. gross necropsy. hematology and clinical chemistry at all doses of both sexes. The period and regularity of sexual cycle were not adversely affected at all doses by the test agent. At 18 mg/kg. both decreased weights of prostate, seminal vesicle and epididymis in males and increased weights of spleen and thymus in females were observed. In addition, decreased number of spermatids and sperms. increased serum testosterone concentration and increased incidence (100%) of interstitial cell hyperplasia were seen in males. At 18 mg/kg of the recovery group. decreased prostate weight. reduced sperm count and increased incidence (20%) of interstitial cell hyperplasia in males and increased thymus weight in females were observed. At 3 mg/kg. reduced sperm count was found. There were no adverse effects on parameters examined at 0.5 mg/kg of both sexes. The results suggested that the potential target organs of flutamide may be accessory sexual glands including testes for males and spleen and thymus for females. Taken together. this test method was found to be a useful screening test system for endocrine disrupting chemicals.

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