• 한국육종학회지
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• 제40권3호
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• pp.250-257
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• 2008

Knowledge of genetic diversity and of the genetic relationships among elite breeding materials has had a significant impact on the improvement of crops. In maize, this information is particularly useful in i) planning crosses for hybrid and line development, ii) in assigning lines to heterotic groups and iii) in plant variety protection. We have used the SSR technique to study the genetic diversity and genetic relationships among 76 Korean waxy corn accessions, representing a diverse collection from throughout Korea. Assessment of genetic diversity among members of this group was conducted using 30 microsatellite markers. Among these 30 microsatellite markers, we identified a total of 127 alleles (with an average of 4.2 and a range of between 2 and 9 alleles per locus). Gene diversity at these 30 microsatellite loci varied from 0.125 to 0.795 with an average of 0.507. The cluster tree generated with the described microsatellite markers recognized two major groups with 36.5% genetic similarity. Group I includes 63 inbred lines, with similarity coefficients of between 0.365 and 0.99. Group II includes 13 inbred lines, with similarity coefficients of between 0.45 and 0.85. The present study indicates that the 30 microsatellite loci chosen for this analysis are effective molecular markers for the assessment of genetic diversity and genetic relationships between Korean waxy corn accessions. Specifically, this study's assessment of genetic diversity and relationships between a set of 76 Korean waxy corn inbred lines will be helpful for such activities as planning crosses for hybrid and line development and association mapping analyses of maize breeding programs in Korea.

• 한국동물위생학회지
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• 제41권4호
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• pp.257-262
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• 2018

Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

• 생명과학회지
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• 제19권2호
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• pp.169-173
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• 2009

본 연구는 마품종 간의 유전적 배경과 다양성을 분석하기 위해 제주마, 경주마, 더러브렛, 몽고마, 쿼터호스, 일본마를 기초 축으로 생물체 집단 간의 유전적 배경 및 관련성을 연구하는데 도움 되는 microsatellite marker를 사용하여 13종의 좌위에 대한 개체별 유전자형을 분석하여 대립 유전자별 빈도에 있어 품종별 차이를 보이는 microsatellite loci들을 나타냈다. 전체 개체들의 유전적 다양성을 나타내는 유전자 좌위별 품종별 기대 이형질성은 0.669-0.869를 나타냈고, 전체 집단 간의 유전자 분화정도는 0.061-0.219를 나타냈다. 이러한 대립 유전자 빈도를 근거로 하여 DISPAN 프로그램을 이용하여 집단 간 표준 유전적 거리를 도식하여 유전자 좌위별 유전적 거리는 0.137-0.414를 나타내어 마품종간의 유전적 다양성과 관련성을 고찰할 수 있게 되었다. 따라서 초위성체 유전자 표지를 이용하여 집단 내의의 혈통분석 및 분자 진화학을 연구하는데 도움이 될 것으로 사료된다.

• Journal of Gastric Cancer
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• 제5권4호
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• pp.260-265
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• 2005

목적: Chk1 kinase는 세포 내 DNA 손상에 따른 세포주기의 저지에 관여하며 G2/M checkpoint에서 중요한 역할을 한다. 연구자들은 위암에서 현미부수체 불안정성과 Chk1 유전자의 격자이동 돌연변이와의 연관성을 알아보고자 하였다. 대상 및 방법: 95예의 위암조직에서 레이저를 이용하여 정상과 암세포를 미세절제한 다음 6개의 현미부수체 표식자를 이용하여 현미부수체 불안정성을 조사하였다. 현미부수체 불안정이 있는 예에서 single strand conformational polymorphism과 염기서열 분석으로 Chk1 유전자의 격자이동 돌연변이를 조사하였다. 결과: 현미부수체 불안정성은 95예의 위암 중 19예 (20%)에서 발견되었는데 고빈도와 저빈도의 현미부수체 불안정성은 각각 13예와 6예였다. 고빈도의 현미부수체 불안정성이 있는 13예 중 2예에서 Chk1 유전자의 격자이동 돌연변이가 발견되었는데 이는 아미노산의 격자이동으로 truncated 단백을 형성하였다. 결론 : 이러한 결과는 현미부수체 불안정성은 Chk1 유전자의 격자이동 돌연변이를 유도하여 세포주기 조절 기능을 상실하게 함으로써 위암의 발생에 관여한다는 것을 의미한다.

• 원예과학기술지
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• 제33권5호
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• pp.737-750
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• 2015

국내에서 시판되고 있는 수박 102 품종에 대한 DNA 프로 파일 데이터베이스를 구축하기 위하여 genomic microsatellite(gMS)와 expressed sequence tag(EST) microsatellite(eMS) 마커의 다형성 정도의 비교와 유전적 연관성 분석을 통한 품종식별력 검정 등에 대한 일련의 연구를 수행하였다. 수박 gMS 마커를 이용하여 국내에서 시판되고 있는 수박 102 품종을 검정하였을 때 마커당 3.63개의 평균 대립유전자가 검출되었으며, 평균 PIC 값은 0.479로 나타났다. 이에 반해 eMS 마커는 평균 대립유전자의 수가 2.50개, PIC 값이 0.425로 나타나 gMS 마커보다 다형성 정도가 낮게 나타났다. gMS와 eMS 및 이들 두 종류의 마커를 병합하여 작성된 계통도는 microsatellite 마커의 다형성에 따라 수박 102개 품종을 6-8개의 그룹으로 구분하였고 대부분의 품종의 식별이 가능하였다. 3가지 마커 유형에 따라 작성된 계통도를 Mantel test에 의해 상관 정도를 분석하였을 때 높은 상관($r{\geq}0.80$)을 나타내었다. 따라서 본 연구에 활용된 microsatellite 마커는 수박 유전자원의 특성평가, 순도검정 및 품종의 지문화 작업의 수단으로 유용하게 활용될 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1402-1405
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• 2003

The QTL(quantitative traits loci) linkage mapping of Hanwoo (Korean Cattle) chromosome 6 for daily gain and marbling score was performed using 378 individuals from 18 paternal half-sib families in Hanwoo. Hanwoo chromosome 6 were mapped to total length of 394.2 cM between 28 microsatellite loci using 36 microsatellite primers of BTA 6 linkage group. The QTL analysis for daily gain in Hanwoo showed 8 microsatellite loci (BM3026-5.66, EL03-5.58, BM4311-5.29, ILSTS035-4.50, BMS1242-4.37, BM1329-3.67, BM415-3.11, BMS2460-3.03) in larger than LOD score 3.0. Based on the QTL analysis for marbling score, LOD scores of 12 microsatellite loci (BM415-8.88, BM3026-7.15, ILSTS093-5.45, ILSTS035-4.91, EL03-4.69, BMS690-4.52, BM1329-4.43, BMS511-3.74, BMS1242-3.66, BMS518-3.65, BM4311-3.41, BMC4203-3.36) were found larger than 3.0.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.25-30
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• 2007

The capability of microsatellite markers for individual identification and their potential for breed assignment of individuals was evaluated in two Indian horse breeds. The strength of these individual assignment methods was also evaluated by increasing the number of loci in increments of five. The probability of identity of two random horses from the two breeds at all twenty five studied loci was as low as $1.08{\times}10^{-32}$ showing their suitability to distinguish between individual horses and their products. In the phylogenetic approach for individual assignment using Nei's genetic distances, 10.81% of horses associated with breed other than the major cluster of the source breed horses when all twenty five microsatellite loci were implemented. Similar results were obtained when the maximum likelihood approach for individual assignment was used. Based on these results it is proposed that, although microsatellite markers may prove very useful for individual identification, their utility for breed assignment of horses needs further evaluation.

• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.916-920
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• 2013

The triploid Pacific oyster, which is produced by mating tetraploid and diploid oysters, is favored by the aquaculture industry because of its better flavor and firmer texture, particularly during the summer. However, tetraploid oyster production is not feasible in all oysters; the development of tetraploid oysters is ongoing in some oyster species. Thus, a method for ploidy verification is necessary for this endeavor, in addition to ploidy verification in aquaculture farms and in the natural environment. In this study, a method for ploidy verification of triploid and diploid oysters was developed using multiplex polymerase chain reaction (PCR) panels containing primers for molecular microsatellite markers. Two microsatellite multiplex PCR panels consisting of three markers each were developed using previously developed microsatellite markers that were optimized for performance. Both panels were able to verify the ploidy levels of 30 triploid oysters with 100% accuracy, illustrating the utility of microsatellite markers as a tool for verifying the ploidy of individual oysters.

• 한국작물학회지
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• 제47권5호
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• pp.379-383
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• 2002

Allelic diversity of 44 microsatellite marker loci originated from the coding regions of specific genes or the non-coding regions of barley genome was analyzed for 19 barley genotypes. Multi-allelic variation was observed at the most of marker loci except for HVM13, HVM15, HVM22, and HVM64. The number of different alleles ranged from 2 to 12 with a mean of 4.0 alleles per micro-satellite. Twenty-one alleles derived from 10 marker loci are specific for certain genotypes. The level of polymorphism (Polymorphic Information Content, PIC) based on the band pattern frequencies among genotypes was relatively high at the several loci such as HVM3, HVM5, HVM14, HVM36, HVM62 and HVM67. In the cluster analysis using genetic similarity matrix calculated from microsatellite-derived DNA profiles, two major groups were classified and the spike-row type was a major factor for clustering. Correlation between genetic similarity matrices based on microsatellite markers and pedigree data was highly significant ($r=0.57^{**}$), but these two parameters were moderately associated each other. On the other hand, RAPD-based genetic similarity matrix was more highly associated with microsatellite-based genetic similarity ($r=0.63^{**}$) than coefficient of parentage.

• Molecules and Cells
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• 제24권1호
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• pp.60-68
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• 2007

Microsatellites, also called simple sequence repeats (SSR), are very useful molecular genetic markers commonly used in crop breeding, species identification and linkage analysis. In the present study, we constructed a microsatellite-enriched genomic library of Panax ginseng, and identified 251 novel microsatellite sequences. Tri-nt repeat units were the most abundant (46.6%), followed by di-nt repeats (35.5%). The $(AG)_n$ motif was most common (23.1%), followed by the $(AAC)_n$ motif (22.3%). From the genotyping of 94 microsatellites using marker-specific primer sets, we identified 11 intraspecific polymorphic markers as well as 14 possible interspecific polymorphic markers differing between P. ginseng and P. quinquefolius. The exact allele structures of the polymorphic markers were determined and the alleles were named. This study represents the first report of the bulk isolation of microsatellites by screening a microsatellite-enriched genomic library in P. ginseng. The microsatellite markers could be useful for linkage analysis, genetic breeding and authentication of Panax species.