• Bulletin of the Korean Chemical Society
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• 제31권4호
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• pp.905-909
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• 2010

Magnetic beads (Dynabeads$^{(R)}$) embedded in ~1 micron size polystyrene beads bearing surface carboxylic acid groups were modified with aminobenzyl ethylenediaminetetraacetic acid (ABEDTA) to concentrate or separate metal ions using pH gradients on micro and nano scales. The immobilization of ABEDTA was achieved by amide formation. The presence of the metal chelating functional group in the fully deprotonated form was confirmed by FT-IR. The chelation efficiency of beads was tested by determining metal ions in supernatant using GFAAS when pH gradients from 3 to 7. Mixtures of Cu and Mg and of Cd and Mn (at 10 ng/mL of metal) were separated as the difference in formation constant with the functional group of ABEDTA. The separation was repeated twice with relative standard deviation of <18%. A polydimethylsiloxane (PDMS) microchip column packed with EDTA-coated magnetic beads was optimized to concentrate metal ion for practical applications by eluting a Cu solution of micro scale at pH 3.

• Bulletin of the Korean Chemical Society
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• 제35권4호
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• pp.1082-1086
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• 2014

A multi-channel microchip electrophoresis (MCME) method with parallel laser-induced fluorescence (LIF) detection was developed for rapid screening of H1N1 virus. The hemagglutinin (HA) and nucleocapsid protein (NP) gene of H1N1 virus were amplified using polymerase chain reaction (PCR). The amplified PCR products of the H1N1 virus DNA (HA, 116 bp and NP, 195 bp) were simultaneously detected within 25 s in three parallel channels using an expanded laser beam and a charge-coupled device camera. The parallel separations were demonstrated using a sieving gel matrix of 0.3% poly(ethylene oxide) ($M_r$ = 8,000,000) in $1{\times}$ TBE buffer (pH 8.4) with a programmed step electric field strength (PSEFS). The method was ~20 times faster than conventional slab gel electrophoresis, without any loss of resolving power or reproducibility. The proposed MCME/PSEFS assay technique provides a simple and accurate method for fast high-throughput screening of infectious virus DNA molecules under 400 bp.

• 한국광학회:학술대회논문집
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• 한국광학회 1999년도 제16회 광학 및 양자전자 학술발표회Proceedings of 16th Optics and Quantum Electronics Conference, 1999
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• pp.82-83
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• 1999

• Bulletin of the Korean Chemical Society
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• 제25권4호
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• pp.560-562
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• 2004

• 대한기계학회논문집A
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• 제30권1호
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• pp.1-7
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• 2006

A system for the electrical bio signal detection for a microchip is proposed. Gold nanoparticles were selected for the system for their bio-compatibility and potential for higher sensitivity with large surface areas. For the estimation of the conductivity of gold nanoparticles, microchips with interdigitated microelectrodes of 3,5,7 and $9\;{\mu}m$ spacing were fabricated. In addition, a simulation program was developed to estimate the electrical resistance of the fabricated microchip. The results of conduction simulation for the nanoparticles show good agreements with experimental data, which validate the proposed system.

• 한국정밀공학회지
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• 제24권1호
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• pp.47-56
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• 2007

Recently, demands on mechanical micro machining technology have been increased in manufacturing of micro-scale precision shapes and parts. The main purpose of this research is to verify the accuracy and cost efficiency of the mechanical micro machining. In order to measure the precision and feasibility of mechanical micro machining, various micro features were machined. Aluminum molds were machined by a 3-axis micro stage in order to fabricate microchips with $200{\mu}m$ wide channel for capillary electrophoresis, then the same geometry of microchip was made by injection molding. To evaluate the cost efficiency of various micro manufacturing processes, cost estimation for mechanical micro machining was conducted, and actual costs of microchips fabricated by mechanical micro machining, injection molding, and MEMS (Micro electro mechanical system) were compared.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제54권10호
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• pp.449-452
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• 2005

We fabricated an electrochemical detector (ECD) to catalyze redox reaction efficiently by electrodepositing Prussian blue (PB) on the indium tin oxide (ITO) electrode. Capillary electrophoresis (CE) and amperometric method were used. We investigated the PB surface properties by topography from atomic force microscopy (AFM). Also PB film thickness calibration with respect to deposition time and voltage was used to get better PB surFace. The PB thin film of dense and smooth surface could catalyze redox reaction efficiently. Comparing with CE-ECD microchip using bare-lTO electrode, proposed CE-ECD microchip using PB deposited electrode has shown better sensitivity by determining the detected peak current from the electropherograms while the concentration of tested analyzes was maintained the same. It is verified that detection limit can be lowered for 0.01 mM of dopamine and catechol respectively.

• 한국기계가공학회지
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• 제20권3호
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• pp.122-128
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• 2021

Machining operations require removal of chips to keep the coolant clean and fresh throughout the operation time. In this study, microchip removal equipment was developed using AutoCAD and CATIA programs for 3D modeling and 2D draft. In addition, the flow analysis and electromagnetic field analysis of the equipment were performed using the COMSOL Multiphysics program. The flow design of the coolant oil tank was realized on the basis of fluid analysis results. Further, on the basis of magnetic density analysis, a conveyer was designed for effectively removing metal microchips in the tank by using arrays of neodymium permanent magnets.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 12th Asian Congress of Fluid Mechanics
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• pp.41.5-42
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• 2008

• 한국광학회:학술대회논문집
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• 한국광학회 2005년도 하계학술발표회
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• pp.52-53
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• 2005