• 한국환경보건학회지
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• 제34권1호
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• pp.86-94
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• 2008

Lake Shiwha, an artificial lake located near metropolitan Seoul, offers a unique water environment and has been suspected to have high levels of chemical and microbiological contaminations. Lake Shiwha was originally connected to the sea but currently has four major surface water inputs from agricultural, municipal, industrial areas and in addition an occasional inflow from the sea. The objectives of this study are to investigate the relative contribution of microbial contaminants from each of the inflowing surface waters and to identify appropriate microbial indicator organisms in this unique water environment. We measured the levels of microbial contaminations in the four inflowing surface waters. A number of microbial indicator organisms including total coliform (TC), fecal coliform (FC), E. coli, Enterococci, somatic and male-specific coliphages were analyzed. Bacterial indicator microorganisms were detected and quantified by the $Colilert^{(R)},\;Enterolert^{(R)}$ kit. Surface water (50 l) was sampled by $ViroCap^{TM}\;5"$ cartridge filters and analyzed by the single agar layer method for detecting coliphages. The concentrations of TC, FC, E. coli, and Enterococci were 1543 CFU/100 ml${\sim}1.99{\times}10^6$ CFU/100 ml, 0 CFU/100 ml${\sim}202$ CFU/100ml, 0 CFU/100 ml${\sim}1.80{\sim}10^5$ CFU/100ml, 74 CFU/100 ml${\sim}3408$ CFU/100 ml, respectively. The male-specific and somatic coliphages were detected in three different inflowing surface waters. Isolated E. coli and Enterococci strains were further analyzed by 16s rDNA amplification and subsequent phylogenetic analysis from Jungwang-chun, Ansan-chun, Banwol-chun and penstock of inflowing surface water. Our results indicated that the concentrations of different fecal indicator microorganisms might not be highly correlated with each other. Multiple microbial indicator organisms should be used for monitoring microbial contamination and microbial source tracking methods.

• 한국식품과학회지
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• 제36권2호
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• pp.365-367
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• 2004

절임 배추의 저장 기간을 연장하기 위하여 배추를 절인 다음 전해산화수로 세척하고 저장하면서 품질 특성의 변화를 측정한 결과는 다음과 같았다. 즉 절임 배추를 전해산화수로 세척한 처리구가 대조구보다 낮은 pH를 보였으며, 저장 8일까지는 대조구에 비하여 낮은 pH를 나타내었다. 적정산도는 저장기간 동안 0.11-0.15%로 큰 변화를 나타내지 않았으며, 대도구와 처리구간에도 큰 차이가 없었다. 절임 배추를 전해산화수로 세척한 직후에는 대조구에 비하여 총균수가 $10^3\;CFU/mL$ 정도 낮았으나 처리구의 총균수는 저장 6일에 대조구와 비슷하였다. 유산균수도 세척 직후에는 처리구가 대조구보다 낮았으며, 저장 4일에는 대조구와 처리구의 차이가 가장 현저하였다. 저장 2일까지는 국물의 밝은 정도(L)에 있어서 대조구와 처리구간에 차이가 없었으나 그 이후에는 처리구가 대조구보다 높은 값을 나타내었다.

• 한국토양비료학회지
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• 제45권6호
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• pp.882-889
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• 2012

대관령 고랭지 농업지대 인근 주요 하천과 도암호의 수질의 이화학성 및 식물성플라크톤의 발생 양상을 조사하여 남한강 상류 수계의 전반적인 수질특성을 파악하고자 하였다. 도암호의 화학적 산소 요구량 (COD)은 $6.1mg\;L^{-1}$ 이고, 총 인 (Total phosphorous)의 함량은 0.26 으로 호소수 생활환경 기준 VI등급 보다 높았다. 부유물질은 평균 9.77 NTU로 호수수 생활기준 보다 높았다. 식물플랑크톤의 농도는 7월부터 9월까지 $2.0{\times}10^3cells\;mL^{-1}$ 이상으로 확인되었는데 이는 여름철 고온과 강우에 의해 영양물질의 대량 유입으로 인해 남조류가 발생하였기 때문인 것으로 생각된다. 온타리오 퇴적물 기준과 비교해 보면, 도암호의 총 질소 및 총 인의 농도는 LEL과 SEL의 중간 정도 값을 보여 오염이 상당부분 진행되어 체계적인 관리로 오염원을 저감 및 차단할 수 있는 방안이 마련되어져야 할 것으로 생각된다.

• 한국환경농학회지
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• 제38권4호
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• pp.254-261
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• 2019

농업용수는 채소류의 식중독세균 오염의 주요 경로 중 하나임에도 불구하고 우리나라에서는 농업용수의 미생물학적 안전성 대한 기초 자료가 부족한 실정이다. 본 연구에서는 전라북도에서 2018년 4월, 7월, 10월에 31지점에서 수집한 지표수 시료와 2018년 4월 7월에 20지점에서 수집한 지하수 시료의 위생지표세균 밀도를 조사하였다. 지표수에서는 평균적으로 대장균군이 2.7±0.55 log CFU/100 mL, 분원성대장균군 1.9±0.71 log CFU/100 mL, 대장균 1.4±0.58 log CFU/100 mL로 나타났고, 7월에 가장 높은 밀도를 보였다. 지하수의 경우 평균적으로 대장균군이 1.9±0.58 log CFU/100 mL, 분원성대장균군 1.4±0.37 log CFU/100 mL, 대장균 1.0±0.33 log CFU/100 mL로 나타났고 조사시기 간의 유의한 차이가 나타나지 않았다. 총질소량(T-N), 질산성질소(NO₃-N) 등 유기물 함량이 높은 용수에서 E. coli O157:H7의 생존이 연장되는 것으로 나타났다. 물에서의 E. coli O157:H7 감소율은 25℃>35℃>5℃>15℃ 순으로 높게 나타났다. 이러한 결과는 전북 지역 농업용수의 미생물학적 오염도와 수질과 온도가 E. coli O157:H7의 생존에 미치는 영향을 보여준다. 이러한 결과는 농업용수의 미생물학적 오염도를 예측하고 미생물 제어 기술 개발의 기초자료로서 활용될 수 있다.

• 한국환경보건학회지
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• 제35권3호
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• pp.162-168
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• 2009

The aim of this study is to investigate microbial sanitary condition of public baths in Seoul, Korea. A total of 28 water samples were collected from 14 different public baths and sudatoriums. The prevalence of fecal indicator microorganisms such as total coliform, fecal coliform, and Escherichia coli was characterized. In addition, bacteria in water was membrane filtered by 0.45um nitrocellulose membrane, and the filter was analyzed by both cultivation and PCR amplification of partial 16S rRNA gene. The levels of chlorine were measured for each of water samples. More than 40% of 14 collected water samples, the concentrations of total coliform bacteria exceeded the water quality for bath water guideline. There was no significant correlation between chlorine residue and the presence of total coliform. Various microorganisms including pathogenic microorganisms were identified from cultivation and subsequent analysis of 16s rRNA gene sequences. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bathhouses.

• 한국식품조리과학회지
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• 제25권5호
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• pp.632-642
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• 2009

In this study the microbiological quality of vegetables was evaluated in relation to the sanitization methods used and vegetable types which consisted of raw food ingredients used in foodservice operations. We analyzed the microbial quantities on lettuce and spinach, which were used as leaf vegetables and cucumber and tomato, which were used as fruit vegetables according to various disinfection methods using different chlorine concentration(50 ppm, 100 ppm, 200 ppm) and exposure time(5 min, 10 min) over 5 days. When the effects of the disinfection methods on microbial qualities and sensory evaluation were analyzed, the following results were obtained. First, in the leaf vegetables, disinfection with a chlorine concentration of 200 ppm for a 5 minutes exposure time was needed to control microbial growth. Second, fruit vegetables sterilized with tap water had reduced microbial qualities after an extended amount of time relative to chlorine disinfection, that is, disinfection with a chlorine concentration of 50 ppm for 5 minutes exposure time was desirable.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.2122-2126
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• 2008

With the degradation of water quality and, at the same time increased water usage, the sources of high quality, for examples, river/stream, municipal reservoir, wells, artisan and surface water, are diminishing. Therefore, the importance of water quality has been emphasized over the years through publications and various literature sources. Even though considerable research has resulted in significant strides for providing interpretive information and mitigation strategies for improvement of waters, the quality of which is still questionable. This study aims to propose a completely independent self-contained system for purifying waters, solar-powered ozone generator. It is a semi-permanent and cost effective environmental solution. Functions of ozone treatment are: 1) to maintain oxidative flexibility, 2) remove harmful chemicals, wastes, and other substances, and 3) prevent epizootic microbial outbreaks. Recent advances in technology have allowed the development of the practical, self-contained and independent solar powered device. Solar electrical producing panels that charge batteries are the key to using these systems anywhere electrical power is not available. This paper invites the readers to examine the problem and consider the viable, proven solution the solar powered ozone purifying system. This paper also introduces basic concept and background of solar powered ozone generators and examine its feasibility for improving water quality in rivers and streams.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.139-139
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• 2022

Nature-based solutions (NBS) involved conservation or rehabilitation of natural ecosystems or the creation of natural processes in modified or artificial ecosystems to mimic natural processes for the improved management of water (UN-Water, 2018). This study investigated the relationship between microbial presence and survival to the pollutant treatment performance of seven different stormwater NBS managing urban stormwater runoff. In this study, seven different stormwater nature-based solution (NBS) was investigated to identify the relationship of microbial community to the pollutant removal performance of stormwater NBS. Based on this study, Proteobacteria was found to be the most dominant microorganism for all stormwater NBS and IS followed by Acidobacteria and Actinobacteria. Acidobacteria, Actinobacteria, Chloroflexi, Gemmatimonadetes, WS3, and AF234118_p were found to have high positive correlation to most pollutant removal efficiency of different stormwater NBS (r-value: 0.62 to 0.68). Using Proteobacteria and Acidobacteria count in stormwater NBS, equations predicting pollutant removal performance were also developed and may be used in minimizing the cost for stormevent monitoring to identify the pollutant removal performance of stormwater NBS.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.201-202
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• 2003

Recently, probiotics is applied for aquaculture management as an alternative method of antibiotics. Probiotics is defined as a live microbial feed supplement which a good effect to the host animal by improving its microbial condition of gastrointestinal tract (Fuller, 1989). Generally, probiotics affect growth and survival of cultured fish, water quality, immune system and so on (Rengpipat et al, 2000, Robertson et al, 2000). (omitted)

• 한국환경보건학회지
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• 제33권6호
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• pp.517-521
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• 2007

To assess possible risks from the consumption of drinking water from various sources, a survey of the microbiological quality of tap water, commercial bottled drinking water which is exploited from natural mineral water, and natural spring water was conducted. A total of 4 different brands of commercial bottled drinking water, and 4 types of spring water from different sources, and tap water from 4 private houses were tested for four index microorganisms, and the microbial quality changes of the water during the storage at room temperature or refrigerated temperature for 7 days. Aerobic plate counts of all of the initial water samples were still within 100 CFU/ml (drinking water standard of Korea). Total coliforms, fecal coliforms, and E. coli were not detected in all of the water samples at initial. However, aerobic plate counts of three types of spring water and three types of bottled drinking water stored at room temperature showed higher levels than the standards in 5 days. Total coliforms were detected in three types of spring water after one day's storage at room temperature, and in one type of bottled drinking water after 5 days' storage. These results indicate that some of the spring water surveyed are not safe to drink, and the spring water and bottled drinking water after opening the lid should not be stored at room temperature, if they are used for drinking.