• Title/Summary/Keyword: Microbial stability

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Effect of Monascus Koji on the Quality Characteristics of Bologna-Type Sausage (홍국 첨가가 소시지 품질특성에 미치는 영향)

  • Rhyu, Mee-Ra;Kim, Eun-Young;Chung, Kyung-Sook
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.229-234
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    • 2003
  • Effects of Monascus koji on color, peroxide value, textural properties, microbial stability, and sensory characteristics of bologna-type sausage during storage at $4^{\circ}C$ for 22 days were studied. Addition of 2% Monascus koji resulted in attractive color and distinctly improved color retention. Lipid oxidation was effectively inhibited by lowering the level of peroxide value. Textural properties and microbial stability of the samples were not affected, whereas color was improved by the addition of Monascus koji. Utilization of Monascus koji in meat product could reduce the use of nitrite and inhibit lipid peroxidation during storage.

The Physicochemical Stabilities and Antimicrobial Activities of Pigment Extracts from Zooshikella sp. 17TA (Zooshikella sp. 17TA 색소 추출물의 물리화학적 안정성과 항균활성)

  • Park, Jae-Myeong;Park, Jin-Sook
    • Journal of Marine Bioscience and Biotechnology
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    • v.11 no.2
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    • pp.89-93
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    • 2019
  • In this study, the stability of the extracted natural pigments against light, temperature, pH, metal ions, and antimicrobial activity were evaluated in marine bacteria Zooshikella sp. 17TA. The pigment of the strain used in the study was red with maximum absorption at a wavelength of 541 nm. The stability of the pigment was evaluated by measuring the absorbance while preserving for 15 days and examining the retention rate. After 15 days of irradiation, the pigment of this bacterium showed 98% retention in the dark and 91% retention in the temperature range of -20℃ ~ 30℃. When the pH was in the range 4-7, the retention was about 80%, and the retention rate was higher than 85% for all kinds of metal ions except for CuCl2, ZnCl2, and KCl. The bacterial pigments showed high stability under the given irradiated pH, temperature, and metal ion conditions and had shown activity against gram-positive strains. These results suggest that this highly conserved microbial pigment can be applied to the food industry.

A Study on the Effect of Expandable Graphite and Metal Hydroxides on the Properties of Wood Plastic Composites (WPCs) (팽창성 흑연과 금속수산화물이 목재·플라스틱 복합재의 특성에 미치는 영향에 관한 연구)

  • Kim, Seungkyun;Lee, Danbee;Lee, Sun-Young;Chun, Sang-Jin;Kim, Birm-June
    • Journal of the Korea Furniture Society
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    • v.27 no.4
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    • pp.392-398
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    • 2016
  • Wood-plastic composites (WPCs) composed of mainly wood flour and thermoplastics have attracted considerable attentions due to advantages of cost effectiveness, high durability, and microbial resistance. However, relatively poor fire resistance of WPCs from low thermal stability of wood and plastics prevents further uses. This study investigated the effect of expandable graphite (EG) and aluminium hydroxide (AH)/magnesium hydroxide (MH) on the properties of WPCs. The combined incorporation of both EG and metal hydroxide (i.e., AH or MH) into formulations leads to higher flexural modulus of filled composites compared to neat PP and WPC. In thermal properties, EG played an important role in improving thermal stability of filled composites by suppressing thermal decompositions of wood and PP. Moreover, EG showed better water absorption features. From this research, it can be said that EG and metal hydroxides have potentials as effective reinforcement, flame retardant, and moisture barrier.

A Comparative Study on the Herbal Prescription and the Herbal Substance of Atractylodis Rhizoma Alba by HPLC analysis (한약제제 백출(白朮)과 한약재 백출에 대한 HPLC 분석 비교연구)

  • Cho, Hyun Joo;Jeon, Yoon Jae;Kim, Nam-Gil;Choi, Hyug-Yong
    • Herbal Formula Science
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    • v.24 no.2
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    • pp.71-79
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    • 2016
  • Objectives : To verify the equivalence between Atractylodis Rhizoma Alba herbal prescription(HP-ARA) and Atractylodis Rhizoma Alba herbal sub stance(HS-ARA). Methods : Safety tests by microbial regulation and heavy metal analysis (total heavy metal, Pb, As) and a stability test by long term shelf test for HP-ARA according to notification of the Ministry of Food and Drug Safety were carried out. Then, multi component profile of HP-ARA and HS -ARA were analyzed by HPLC. Results : The safety and stability of HP-ARA confirmed by several tests. Correlation coefficient of equivalence of HP-ARA and ARA-HS showed 0.992. Conclusion : Based on this result of equivalence between HP-ARA and HS-ARA, HP-ARA can substitute HS-ARA used to make herbal medicines (herbal decoction, pills and powder).

Effects of Whey Protein Injection as a Curing Solution on Chicken Breast Meat

  • Ha, Jung-Heun;Lee, Ju-Ho;Lee, Jae-Joon;Choi, Yang-Il;Lee, Hyun-Joo
    • Food Science of Animal Resources
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    • v.39 no.3
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    • pp.494-502
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    • 2019
  • The quality characteristics and storage stability of chicken breast meat (CBM) was investigated following the injection of whey protein (WP) as a curing ingredient. The moisture content of CBM decreased with increasing concentration of WP. The highest concentration of WP (7%) resulted in the lowest moisture and fat content and the highest protein content of CBM. Injection of WP elevated the pH and water holding capacity (WHC) of CBM. The cooking loss of CBM was significantly decreased with WP injections of 3% and higher. All WP injections increased the $L^*$ of the CBM but decreased the $a^*$ and $b^*$. WP injection increased the springiness, cohesiveness, and chewiness and decreased the hardness of the CBM. WP injection increased 2-thiobarbituric acid reactive substances (TBARS) after 3 and 7 days of storage. The volatile basic nitrogen (VBN) content of the CBM increased with increased concentrations of WP. The total microbial count (TMC) of CBM injected with WP was higher initially and after 3 days of storage. Our results showed WP injection improved the WHC of CBM but decreased the storage stability by increasing TBARS, VBN and TMC.

Supercooling Pretreatment Improves the Shelf-Life of Freeze-Dried Leuconostoc mesenteroides WiKim32

  • Seul-Gi Jeong;In Seong Choi;Ho Myeong Kim;Ji Yoon Chang;Hae Woong Park
    • Journal of Microbiology and Biotechnology
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    • v.32 no.12
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    • pp.1599-1604
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    • 2022
  • Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

Estimating variation in the microbiological quality of seasoned soybean sprouts using probability model (확률 모형을 이용한 콩나물 무침의 미생물적 품질 변화 예측)

  • Park, Jin-Pyo
    • Journal of the Korean Data and Information Science Society
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    • v.21 no.5
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    • pp.909-916
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    • 2010
  • This study aims to establish storage stability conditions for cook-chilled korean ethenic foods. In order to achieve this aims, we establish a probability model of microbial counts of cook-chilled korean side dishes product-seasoned soybean sprouts. And seasoned soybean sprouts were stored during 1 to 5 days under constant temperature conditions at 0, 5, 10 and $15^{\circ}C$. Next we find confidence intervals for variation in the microbiological quality of seasoned soybean sprouts.

Asn124 of Cel5A from Hypocrea jecorina not only provides the N-glycosylation site but is also essential in maintaining enzymatic activity

  • Qin, Yuqi;Qu, Yinbo
    • BMB Reports
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    • v.47 no.5
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    • pp.256-261
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    • 2014
  • To investigate the function of N-glycosylation of Cel5A (endoglucanase II) from Hypocrea jecorina, two N-glycosylation site deletion Cel5A mutants (rN124D and rN124H) were expressed in Saccharomyces cerevisiae. The weights of these recombinant mutants were 54 kDa, which were lower than that of rCel5A. This result was expected to be attributed to deglycosylation. The enzyme activity of rN124H was greatly reduced to 60.6% compared with rCel5A, whereas rN124D showed slightly lower activity (10%) than that of rCel5A. rN124D and rN124H showed different thermal stabilities compared with the glycosylated rCel5A, especially at lower pH value. Thermal stabilities were reduced and improved for rN124D and rN124H, respectively. Circular dichroism spectroscopy showed that the modification of secondary structure by mutation may be the reason for the change in enzymatic activity and thermal stability.

Real-time Water Quality Prediction for Evaluation of Influent Characteristics in a Full-scale Sewerage Treatment Plant (하수처리장 유입수의 특성평가를 위한 실시간 수질예측)

  • Kim, Youn-Kwon;Chae, Soo-Kwon;Han, In-Sun;Kim, Ju-Hwan
    • Journal of Environmental Impact Assessment
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    • v.19 no.6
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    • pp.617-623
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    • 2010
  • It is the most important subject to figure out characteristics of the wastewater inflows of sewerage treatment plant(STP) when situation models are applied to operation of the biological processes and in the automatic control based on ICA(Instrument, Control and Automation). For the purposes, real-time influent monitoring method has been applied by using on-line monitoring equipments for the process optimization in conventional STP. Since, the influent of STP is consist of complex components such as, COD, BOD, TN, $NH_4$-N, $NO_3$-N, TP and $PO_4$-P. MRA2(Microbial Respiration Analyzer 2), which is capable of real-time analyzing of wastewater characteristics is used to overcome the limitations and defects of conventional online monitoring equipments in this study. Rapidity, accuracy and stability of developed MRA2 are evaluated and compared with the results from on-line monitoring equipments for seven months after installation in Full-scale STP.

A study on the Development of Standardized Recipe and the Microbiological Assessment and Sensory Evaluation of Various Bulkogis for Steam Convection Oven and Cook/Chill System for Kindergarten Foodservice Operations (유치원 급식에 적용하기 위한 불고기류의 Steam Convection Oven 및 Cook/Chill System용 레시피 개발 및 미생물적, 관능적 품질 평가에 대한 연구)

  • 강현주;김경자;김은희
    • Korean journal of food and cookery science
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    • v.14 no.4
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    • pp.358-366
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    • 1998
  • To meet the demand for high quality of foods with the expanded implementation of foodservice into kindergarten, and to make the efficient use of resources, the necessity of introducing central production unit with cook/chill system into kindergarten foodservice is getting increased. The purpose of this study were to develop standardized recipe applicable to cook/chill system for kindergarten foodservice and to evaluate the microbial and sensory quality. For quantitty production of cook/chill system in kindergarten foodservice, Various Bulkogis were selected as menu items. The followings are summary of the results: 1. Standard recipes to produce Various Bulkogis(Broiled Sliced Meat with Sauce) for cook/chill system have been developed. 2. The microbial quality of Various Bulkogis during 10 days chilled storage was very good. And in this experiment, the stability of storage of standard recipes of Various Bulkogis at cook/chill system was proved for 10 days. 3. All of the items of sensory evaluation of Bulkogis has no significance between the first day and the third day of storage, except the color of Pork Bulkogi (P<0.05).

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