• Title/Summary/Keyword: Microbial removal

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The study on Fixed Bio-reactor Characteristics Using Porous Media (다공성 여재를 이용한 고정생물막 반응기 특성에 관한 연구)

  • 이영신;김동민;정상철;백명석
    • Journal of Environmental Health Sciences
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    • v.22 no.1
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    • pp.99-106
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    • 1996
  • The purpose of this study is to observe a specific removal efficiency of synthethetic wastewater which is managed by upflow submerged type at porous media which was sinteringed on a comparative low temperature 600$\circ$C, was annexed slag and humus soil with main material kaolinite. Observing removal efficiency quality of each media, a mixed media of kaolinite and humus soil by gravity percent 60, 40% respectively showed the most excellent removal utility, and applied predictive models for suspended culture kinetics without consideration diffusion limitation, and when analyzed kinetic which had been processed by this study the removal efficiency accompanied by carbon, nitrogen, phosphorous volumetric loading rate variation standed for a comparative large change rate 61~71%, it means the selection of the most proper load factor had a great effect on the highly removal efficiency, yield coefficient(Y) and specific microbial attach equation showed 1.53 mgVSS/mgCOD, $m_p=10039.4\times ((S_0)/(6.75+S_0))$ repectively.

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Treatment of Fish Processing Wastewater Using Sequencing Batch Reactor (SBR) (연속회분식 반응기를 이용한 수산물 가공폐수 처리)

  • Paik, Byeong Cheon;Shin, Hang Sik
    • Journal of Korean Society of Water and Wastewater
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    • v.8 no.1
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    • pp.18-26
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    • 1994
  • This research investigated efficient operation mode for the successful performance of SBR(sequencing batch reactor) treating fish processing wastewater, and the effect of sodium chloride (NaCl) on treatment efficiency. 2-hour-annerobic, 6-hour-aerobic and 3-hour-anoxic operation during reaction period was found an effective operating method for organic and nitrogen removal from fish processing wastewater in SBR system. The average removal efficiencies of COD, BOD, and total nitrogen in SBR operated continuousely were 91%, 95%, and 67.1%, respectively. The estimated values of biomass yield coefficient(Y), microbial decay coefficient($K_d$), and bioreaction rate constant(K) were $0.35gMLSS/gCOD_{removed}$, $0.015day^{-1}$, and $0.209hr^{-1}$, respectively. As NaCl concentration increased from 5 to 30g/L, sludge settleability was cnhanced but organic removal in the reactor was decreased. NaCl of influent had considerable relationship with COD removal, whereas it did not significant affect nitrogen removal.

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Removal Characteristics of Cyclic Ethers in Biological Wastewater Treatment System (고리형 에테르의 생물학적 처리 특성)

  • Lee, Sung-Ryul;Jeong, Yeon-Koo
    • Journal of Environmental Science International
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    • v.17 no.3
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    • pp.343-350
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    • 2008
  • The fate of two cyclic ethers, THF(Tetrahydrofuran) and 1,4-Dioxane, in conventional biological wastewater treatment plants was investigated using sequential activated sludge process. Removal efficiency of THF were about 86% in average, which was greater than that of 1,4-Dioxane, 30%. However, it was not clear whether the removal of cyclic ethers in biological system was caused by microbial activity or not. Thus treatability tests were conducted by batch experiments. The effects of mixing, aeration and the addition of activated sludge on the removal of cyclic ethers were investigated in batch experiments. THF was totally removed by mixing and aeration in 24 hours while removal ratio of 1,4-Dioxane was at most 30% for the same period. This results could be ascribed to the differences in Henry's law constants between the two chemicals. In addition, biological degradation including biosorption was not obviously observed in these batch tests.

Simultaneous N-P Removal of Wastewater with Flow Variation by Anaerobic-Aerobic Activated Sludge Process(I) (혐기-호기 활성슬러지법에 의한 유량변동이 있는 폐수의 N-P 동시 제거에 관한 연구(I))

  • 이민규;서근학
    • Journal of Environmental Science International
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    • v.4 no.5
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    • pp.509-516
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    • 1995
  • The treatment performances of anaerobic-aerobic activated sludge process were investigated under various operation conditions. The treatment system proposed in this study gave a relatively stable performance against hourly change of the flow rate and showed a satisfactory removal of nitrogen and phosphorus compounds under experimental conditions. The recycle ratio of mixed liquor from aerobic to anaerobic region and peak coefficient primarily controlled the extent of nitrogen removal. The recycle ratio had the optimum values which were determined by the microbial activities of nitrification and denitrification. The behavior of the treatment unit could be simulated by using the kinetic equations and reactor models which considered the treatment units as complete mixing tanks.

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Simultaneous N-P Removal of Wastewater with Flow Variation by Anaerobic-Aerobic Activated Sludge Process(I) (혐기-호기 활성슬러지법에 의한 유량변동이 있는 폐수의 N-P 동시 제거에 관한 연구(I))

  • Lee, Min-Gyu;Suh, Kuen-Hack
    • Journal of Environmental Science International
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    • v.4 no.5
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    • pp.123-123
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    • 1995
  • The treatment performances of anaerobic-aerobic activated sludge process were investigated under various operation conditions. The treatment system proposed in this study gave a relatively stable performance against hourly change of the flow rate and showed a satisfactory removal of nitrogen and phosphorus compounds under experimental conditions. The recycle ratio of mixed liquor from aerobic to anaerobic region and peak coefficient primarily controlled the extent of nitrogen removal. The recycle ratio had the optimum values which were determined by the microbial activities of nitrification and denitrification. The behavior of the treatment unit could be simulated by using the kinetic equations and reactor models which considered the treatment units as complete mixing tanks.

Lab sacle의 섬유상담체를 이용한 VOCs 제거

  • Jang, Jeong-Gyun;Choe, Hwan-Seok;Park, Ju-Yeong;Cha, Jin-Myeong;O, Min-Ha;Park, Don-Hui
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.552-555
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    • 2001
  • This work reported concerns the removal of mixtures of methyl ethyl ketone (MEKJ, methyl isobutyl ketone (MIBK) and BTXs, which find wide application as industrial solvents, using the biofilter by the microbial consortium, The biofilter was constructed from acryl columns and was 400 mm in length and 55 mm in diameter and the height of fibrous packing material which made of PVC was 160 111111, 8 seconds of the retention time, pH 6.5 - 7.5 and the initial inlet concentration of MEK, MIBK and BTXs were 220 ppm. The removal efficiency of the gaseous mixtures was relatively low during the initial 2 days after inoculum of the microbial consortium, after 3 days, however, the efficiency was increased remarkably. In this study, The removal efficiency of the biofilter for the mixtures show the high degree from one day after inoculum of the microbial consortium, having no relation to the fluctuation of the inlet concentration of MEK, MIBK and BTXs.

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Performance and Spatial Succession of a Full-Scale Anaerobic Plant Treating High-Concentration Cassava Bioethanol Wastewater

  • Gao, Ruifang;Yuan, Xufeng;Li, Jiajia;Wang, Xiaofen;Cheng, Xu;Zhu, Wanbin;Cui, Zongjun
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1148-1154
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    • 2012
  • A novel two-phase anaerobic treatment technology was developed to treat high-concentration organic cassava bioethanol wastewater. The start-up process and contribution of organics (COD, total nitrogen, and $NH_4^+$-N) removal in spatial succession of the whole process and spatial microbial diversity changing when sampling were analyzed. The results of the start-up phase showed that the organic loading rate could reach up to $10kg\;COD/m^3d$, with the COD removal rate remaining over 90% after 25 days. The sample results indicated that the contribution of COD removal in the pre-anaerobic and anaerobic phases was 40% and 60%, respectively, with the highest efficiency of 98.5%; TN and $NH_4^+$-N had decreased to 0.05 g/l and 0.90 g/l, respectively, and the mineralization rate of total nitrogen was 94.8%, 76.56% of which was attributed to the anaerobic part. The microbial diversity changed remarkably among different sample points depending on the physiological characteristics of identified strains. Moraxellaceae, Planococcaceae, and Prevotellaceae were dominant in the pre-anaerobic phase and Bacteroidetes, Campylobacterales, Acinetobacter, Lactobacillus, Clostridium, and Bacillus for the anaerobic phase. Methanosarcinaceae and Methanosaeta were the two main phylotypes in the anaerobic reactor.

ENHANCED BIOREMEDIATION AND MODIFIED BACTERIAL COMMUNITY STRUCTURE BY BARNYARD GRASS IN DIESEL-CONTAMINATED SOIL

  • Kim, Jai-Soo;Min, Kyung-Ah;Cho, Kyung-Suk;Lee, In-Sook
    • Environmental Engineering Research
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    • v.12 no.2
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    • pp.37-45
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    • 2007
  • Phytoremediation has been used effectively for the biodegradation of oil-based contaminants, including diesel, by the stimulation of soil microbes near plant roots (rhizosphere). However, the technique has rarely been assessed for itsinfluence on soil microbial properties such as population, community structure, and diversity. In this study, the removal efficiency and characteristics of rhizobacteria for phytoremediation of diesel-contaminated soils were assessed using barnyard grass (Echinochloa crusgalli). The concentration of spiked diesel for treatments was around $6000\;mg\;kg^{-1}$. Diesel removal efficiencies reached 100% in rhizosphere soils, 76% in planted bulk soils, and 62% in unplanted bulk soils after 3weeks stabilization and 2 months growth(control, no microbial activity: 32%). The highest populations of culturable soil bacteria ($5.89{\times}10^8$ per g soil) and culturable hydrocarbon-degraders($5.65{\times}10^6$ per g soil) were found in diesel-contaminated rhizosphere soil, also yielding the highest microbial dehydrogenase. This suggests that the populations of soil bacteria, including hydrocarbon-degraders, were significantly increased by a synergistic rhizosphere + diesel effect. The diesel treatment alone resulted in negative population growth. In addition, we investigated the bacterial community structures of each soil sample based on DGGE (Denaturing Gel Gradient Electrophoresis) band patterns. Bacterial community structure was most influenced by the presence of diesel contamination (76.92% dissimilarity to the control) and by a diesel + rhizosphere treatment (65.62% dissimilarity), and least influenced by the rhizosphere treatment alone (48.15% dissimilarity). Based on the number of distinct DGGE bands, the bacterial diversity decreased with diesel treatment, but kept constant in the rhizosphere treatment. The rhizosphere thus positively influenced bacterial population density in diesel-contaminated soil, resulting in high removal efficiency of diesel.

Nitrogen Removal in Flat-Panel Air-Cathode Microbial Fuel Cell according to Various Inoculum Sources and Organic Concentration (식종원 및 유기물 농도 변화에 따른 평판형 외기환원전극 미생물 연료전지의 질소 제거)

  • Park, Younghyun;Yu, Jaecheul;Nguyen, Thi Hien;Lee, Taeho
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.12
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    • pp.635-640
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    • 2016
  • Although microbial fuel cell (MFC) can produce electricity from organics in wastewater, nitrogen removal is required for application of process for wastewater treatment plant. This study developed flat-panel air-cathode MFCs (FA-MFCs) comprised of two large separator electrode assemblies (SEAs) and evaluate total nitrogen removal according to three inoculum sources and pre-nitrification acclimation. The nitrification efficiencies were >99% regardless of inoculum sources under the phase for pre-nitrification acclimation. The total nitrogen removal efficiencies of FA-MFCs without pre-nitrification acclimation were the highest at the low organic conditions (<300 mg-COD/L) under the phase for nitrification and denitrification. The increase of organic concentration influenced the total nitrogen removal efficiency, positively. The organics were removed >95% but were not used for heterotrophic denitrification totally. This study suggests that application of FA-MFC system for wastewater treatment can allow the simultaneous removal of organic and nitrogen compounds, although this affects the low electricity production.

Microbial Community Analysis of 5-Stage Biological Nutrient Removal Process with Step Feed System

  • Park, Jong-Bok;Lee, Han-Woong;Lee, Soo-Youn;Lee, Jung-Ok;Bang, Iel-Soo;Park, Eui-So;Park, Doo-Hyun;Park, Yong-Keun
    • Journal of Microbiology and Biotechnology
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    • v.12 no.6
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    • pp.929-935
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    • 2002
  • The 5-stage biological nutrient removal (BNR) process with step feed system showed a very stable organic carbon and nutrient removal efficiency ($87\%\;COD\,;79\%\;nitrogen,\;and\;87\%$ phosphorus) for an operation period of 2 years. In each stage at the pilot plant, microbial communities, which are important in removing nitrogen and phosphorus, were investigated using fluorescence in-situ hybridization (FISH) and 165 rDNA characterization. All tanks of 5-stage sludge had a similar composition of bacterial communities. The totat cell numbers of each reactor were found to be around $2.36-2.83{\times}10^9$ cells/ml. About $56.5-62.0\%$ of total 4,6-diamidino-2-phenylindol (DAPI) cells were hybridized to the bacterial-specific probe EUB388. Members of ${\beta}$-proteobacteria were the most abundant proteobacterial group, accounting for up to $20.6-26.7\%$. The high G+C Gram-positive bacterial group and Cytophaga-Flexibacter cluster counts were also found to be relatively high. The beta subclass proteobacteria did not accumulate a large amount of polyphosphate. The proportion of phosphorus-accumulating organisms (PAOs) in the total population of the sludge was almost $50\%$ in anoxic-1 tank. The high G+C Gram-positive bacteria and Cytophaga-Flexibacter cluster indicate a key role of denitrifying phosphorus-accumulating organisms (dPAOs). Both groups might be correlated with some other subclass of proteobacteria for enhancing nitrogen and phosphorus removal in this process.