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http://dx.doi.org/10.4491/eer.2007.12.2.037

ENHANCED BIOREMEDIATION AND MODIFIED BACTERIAL COMMUNITY STRUCTURE BY BARNYARD GRASS IN DIESEL-CONTAMINATED SOIL  

Kim, Jai-Soo (Department of Life Science, Kyonggi University)
Min, Kyung-Ah (Department of Environmental Science and Engineering, Ewha Womans University)
Cho, Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
Lee, In-Sook (Department of Life Science, Ewha Womans University)
Publication Information
Environmental Engineering Research / v.12, no.2, 2007 , pp. 37-45 More about this Journal
Abstract
Phytoremediation has been used effectively for the biodegradation of oil-based contaminants, including diesel, by the stimulation of soil microbes near plant roots (rhizosphere). However, the technique has rarely been assessed for itsinfluence on soil microbial properties such as population, community structure, and diversity. In this study, the removal efficiency and characteristics of rhizobacteria for phytoremediation of diesel-contaminated soils were assessed using barnyard grass (Echinochloa crusgalli). The concentration of spiked diesel for treatments was around $6000\;mg\;kg^{-1}$. Diesel removal efficiencies reached 100% in rhizosphere soils, 76% in planted bulk soils, and 62% in unplanted bulk soils after 3weeks stabilization and 2 months growth(control, no microbial activity: 32%). The highest populations of culturable soil bacteria ($5.89{\times}10^8$ per g soil) and culturable hydrocarbon-degraders($5.65{\times}10^6$ per g soil) were found in diesel-contaminated rhizosphere soil, also yielding the highest microbial dehydrogenase. This suggests that the populations of soil bacteria, including hydrocarbon-degraders, were significantly increased by a synergistic rhizosphere + diesel effect. The diesel treatment alone resulted in negative population growth. In addition, we investigated the bacterial community structures of each soil sample based on DGGE (Denaturing Gel Gradient Electrophoresis) band patterns. Bacterial community structure was most influenced by the presence of diesel contamination (76.92% dissimilarity to the control) and by a diesel + rhizosphere treatment (65.62% dissimilarity), and least influenced by the rhizosphere treatment alone (48.15% dissimilarity). Based on the number of distinct DGGE bands, the bacterial diversity decreased with diesel treatment, but kept constant in the rhizosphere treatment. The rhizosphere thus positively influenced bacterial population density in diesel-contaminated soil, resulting in high removal efficiency of diesel.
Keywords
Phytoremediation; Rhizosphere; Bulk; Population; Diversity; DGGE (Denaturing Gel Gradient Electrophoresis);
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