• 한국환경보건학회지
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• 제35권3호
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• pp.162-168
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• 2009

The aim of this study is to investigate microbial sanitary condition of public baths in Seoul, Korea. A total of 28 water samples were collected from 14 different public baths and sudatoriums. The prevalence of fecal indicator microorganisms such as total coliform, fecal coliform, and Escherichia coli was characterized. In addition, bacteria in water was membrane filtered by 0.45um nitrocellulose membrane, and the filter was analyzed by both cultivation and PCR amplification of partial 16S rRNA gene. The levels of chlorine were measured for each of water samples. More than 40% of 14 collected water samples, the concentrations of total coliform bacteria exceeded the water quality for bath water guideline. There was no significant correlation between chlorine residue and the presence of total coliform. Various microorganisms including pathogenic microorganisms were identified from cultivation and subsequent analysis of 16s rRNA gene sequences. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bathhouses.

• 한국포장학회지
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• 제3권2호
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• pp.3-16
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• 1996

This study was investigated to the effect of oxygen barrier of four different packaging films of the shelf life of proessed meat product by the simulated distribution test and storage test. The microbial growth, pH, VBN content, TBA value, color change and sensory evaluation were compared during the 2-month storage period at 4, 10, $20^{\circ}C$ after finished the simulated distribution test. The oxygen transmission rates ($cc/m^2$ day) were BF001: 27,88; BF003; 12.30;NY; 77.98; EVOM; 0.41 respectively. The packaging films were not effected on the changes of pH, VBN content, TBA value and color during the storage period. The aerobic microbial growth was increased only at $20^{\circ}C$ in the order of growth rate in the following films;NY > BF001 > BF003 > EVOH. Therefore, the microbial growth was affected significantly by the properties of oxygen barrier off packaging films and it should be an indicator of establishing the shelf life processed meat product.

• 한국환경과학회지
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• 제28권7호
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• pp.629-637
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• 2019

The purpose of this study was to evaluate the effect of high-salinity wastewater on the microbial activity of Aerobic Granule Sludge (AGS). Laboratory-scale experiments were performed using a sequencing batch reactor, and the Chemical Oxygen Demand (COD), nitrogen removal efficiency, sludge precipitability, and microbial activity were evaluated under various salinity injection. The COD removal efficiency was found to decrease gradually to 3.0% salinity injection, and it tended to recover slightly from 4.0%. The specific nitrification rate was 0.043 - 0.139 mg $NH_4{^+}-N/mg$ $MLVSS{\cdot}day$. The specific denitrification rate was 0.069 - 0.108 mg $NO_3{^-}-N/mg$ $MLVSS{\cdot}day$. The sludge volume index ($SVI_{30}$) ultimately decreased to 46 mL/g. The specific oxygen uptake rate decreased from an initial value 120.3 to a final value 70.7 mg $O_2/g$ $MLVSS{\cdot}hr$. Therefore, salinity injection affects the activity of AGS, causing degradation of the COD and nitrogen removal efficiency. It can be used as an indicator to objectively determine the effect of salinity on microbial activity.

• 한국지하수토양환경학회지:지하수토양환경
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• 제28권5호
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• pp.12-24
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• 2023

The Songji lagoon is brackish environment with a mixture of saline and fresh water, and the interaction of groundwater-lagoon water creates a physicochemical gradient. Although some studies have been conducted on the hydrological and geochemical characteristics of the Songji lagoon, microbial ecological studies have not yet been conducted. In this study, we investigated the effect of groundwater and surface water interaction on water quality as well as microbial community changes in the Songji Lagoon using 16S rRNA gene sequencing. Hydrochemical analyses show that samples were classified as 5 hydrochemical facies (HF) and hydrochemical facies evolution (HFE) revealed the intrusion phase was more dominant (57.9%) than the freshening phase (42.1%). Higher microbial diversity was found in freshwater in comparison to saline water samples. The microbial community at the phylum level shows the most dominance of Proteobacteria with an average of 37.3%, followed by Bacteroidota, Actinobacteria, and Patescibacteria. Heat map analyses of the top 18 genera showed that samples were clustered into 5 groups based on type, and Pseudoalteromonas could be used potential indicator for seawater intrusion.

• Applied Biological Chemistry
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• 제49권2호
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• pp.158-162
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• 2006

두부의 저장 중 신선도를 판정 할 수 있는 신선도표시계를 제작하여 두부의 표면에 부착하여 품질변화를 측정하였다. 부패시점을 판정하기 위하여 pH, VBN, TBARS, 총균수 및 관능검사를 수행하였다. 두부의 저장 중 품질변화를 측정한 결과 신선도표시계의 반응 시점과 같은 저장 8일째에 두부는 부패초기수준에 도달하여 pH는 5.6, 총균수는 7.63 log CFU/g의 값을 나타내었다. 또한 VBN, TBARS 값도 상승하였고, 관능검사 결과도 저장 8일 이후에는 이취 및 표면에 점질물 생성 및 조직의 저하 등 가식이 불가능 하였다. 따라서 본 연구에서 사용된 신선도표시계는 두부의 저장 중 신선도를 실시간으로 나타내줌으로써 두부의 유통기한 확립 측면에서 매우 유용한 도구가 될 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1709-1715
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• 2021

Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCR-based technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater.

• 한국환경보건학회지
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• 제22권4호
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• pp.109-121
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• 1996

It was intended to investigate the effect of the microbiological kitchen hygiene such as dishclothes and scrubbers. The 8indicator organisms (standard plate counts, coliform, heterotroph, enterococcus, staphylococcus, heat-stable bacteria, psychrotroph, Pseudomonas aeruginosa) were detected highly in dishwaters, dishcloth and scrubber. Coliform and Staphylococcus aureus were appeared on dishcloth dominantly than the scrubber, and the scrubbers were intruded by hetrotrophs and psychrotrophs numerously than dishclothes. The germ-resistant sponge inhibited the growth of the most of test strain, and appeared the about 100% reduction rate after 24 hr, but did not affect Pseudomonas aeruginosa and P. fragi so typically after 24 hr. The anti-microorganism durability of germ-resistant sponge, treated with food soil, was maintained by 10 days, the early stage strain density was founded in 20 days, and the strains grew after 30 days.

• 대한환경공학회지
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• 제32권4호
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• pp.363-368
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• 2010

본 연구에서는 아데노신3인산의 측정을 통하여 음식물 쓰레기의 발효-소멸반응에서의 미생물이 발생 및 활성정도를 총괄할 수 있는 인자를 도출할 수 있었다. 목질바이오칩을 이용한 음식물 쓰레기의 발효-소멸에서 무게감량 및 유기물 분해율이 높아질수록 아데노신3인산의 농도가 증가하였다. 아데노신3인산은 목질바이오칩의 종류별 유기물 분해 및 무게 감량에 대한 성능 선택성 설정에 유용한 인자로 활용 기대된다.

• KSBB Journal
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• 제19권5호
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• pp.390-393
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• 2004

Oxygen uptake rate (OUR) was used as an indicator of microbial activity. In this study OUR at dyeing wastewater in the pilot plant was monitored to examine biological activity. Correlation between inlet COD concentration and maximum OUR showed that maximum OUR was proportional to inlet COD concentration. Changes in the OUR values reflected the changing waste load in the reactor. Consequently, OUR can be used to estimate biological activity of inlet COD concentration. This study showed that biodegradable COD at dyeing wastewater could be calculated from OUR and yield coefficient. Non-biodegradable COD was able to be calculated from a difference between initial COD concentration and biodegradable COD.

• 한국식품위생안전성학회지
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• 제33권6호
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• pp.447-452
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• 2018

유해 미생물에 오염된 농업용수는 배추의 재배기간 동안 지속적인 식중독세균 오염의 주원인이 될 수 있다. 농업용수는 야생동물 및 가축의 분변에 의해 오염되고 있다. 따라서 본 연구는 야생동물의 출입이 농업용수의 미생물학적 안전성에 미치는 영향을 조사하기 위하여 수행하였다. 이를 위하여 산악지대에 위치한 배추 재배농가에 사용되는 농업용수의 상류에서부터 하류까지 시기별 위생지표세균 오염도와 수원 상류의 야생동물의 출입 빈도를 조사하였다. 배추를 재배하는 기간 동안 멧돼지, 고라니 등 총 37회의 야생동물들이 농업용수 수원 근처에서 관찰되었다. 농업용수의 위생지표세균을 조사한 결과, 3종의 위생지표세균 모두 사람의 출입이 없는 상류에서부터 검출되었으며 대장균군, 대장균, 장구균의 오염도는 각각 2.13~4.32 log MPN/100mL, 0.26~2.03 log MPN/100mL, 1.43~3.49 log MPN/100mL 수준이었다. 대장균군과 대장균의 오염수준은 배추 이식기 보다 수온이 낮은 수확기에 낮았으나 장구균은 시기별로 큰 차이가 없었다. 본 연구의 결과로 미루어 볼 때 농업용수의 오염은 야생동물의 출입과 관련이 있을 것으로 판단된다.