• Asian-Australasian Journal of Animal Sciences
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• v.4 no.2
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• pp.161-164
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• 1991

A rapid and accurate method is described for the determination of nucleo-bases in rumen micro-organisms. A procedure to satisfactorily hydrolyse the micro-organisms involving reaction with a mixture of readily volatile organic acids (acetic and formic acids) under high pressure, is proposed, and optimal conditions for an analytical procedure with reversed phase HPLC is described. The following nucleobases contents (mmol/kg DM) of rumen micro-organisms were found: Adenine (Ade), 82.62; Guanine (Gua), 61.34; Cytosine (Cyt), 84.61; Thymine (Thy), 35.74; Uracil (Ura), 68.62; Hypoxanthine (Hxn), 13.06; Xanthine (Xn), 8.35. Total purine-N content (g/kg N) of rumen micro-organisms were 99.60. The nucleic acid N content (g/kg N) of microbial isolates were: RNA-N, 109.9; DNA-N, 50.9.

• Journal of the Korea Concrete Institute
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• v.23 no.3
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• pp.331-338
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• 2011

The purpose of this study is to use organisms or micro-organism functions for eco-friendly water-purification of cement bricks, utilizing bioremediation. Many researches have been performed in the past to improve water quality by using effective micro-organisms in construction materials. In order to purify water using micro-organisms, this research used soybean paste bacteria, an effective micro-organism that was identified through 16S rDNA sequence analysis performed in Daegu S. Environment Protection Institute in addition to Natto bacteria that was studied in the previous research. With these effective micro-organisms with water-purification ability, this study examined their water-purification possibility on cement bricks. This study used Zeolite to immobilize micro-organisms to bricks, and confirmed that the micro-organisms were attached on Zeolite from SEM analysis. The experimental results showed that specific micro-organisms can be used to effectively remove contamination an used to develop eco-friendly construction materials. The study on micro-organisms for material purification shows great promises as a future research topic.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.35-42
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• 2015

Purpose: The present study was aimed to compare the difference in adherence level of microorganisms according to contact lens materials and protein deposition and to evaluate disinfection efficacy of multipurpose solution. Methods: The evaluations of micro-organisms' adherence and disinfection efficacy of multi-purpose solution were conducted by employing the Part 2. Regimen Procedure for Disinfecting Regiments in the Disinfection Efficacy Testing under the "FDA Evaluation Criteria & Method". Results: Pseudomonas aeruginosa, Serratia marcescens, Candida albicans except Staphylococcus aureus adhered more on etafilcon A lens and disinfection efficacy of total 4 products investigated was almost perfect except Candida albicans. The 3 micro-organisms except Serratia marcescens adhered more to albumin-predeposited lens. Disinfection efficacy of multi-purpose solution was higher against the micro-organisms adhered to albumin-deposited lens than against the micro-organisms adhered to the lysozyme-deposited lens. Furthermore, disinfection efficacy of multi-purpose solution was different according to types of micro-organisms. Conclusions: It was revealed that the type of micro-organisms, the lens materials and type of absorbed tear protein affected the amount of adhered micro-organisms to contact lens and that adhesion of tear protein could induce the change of disinfection efficacy of multi-purpose solution. It suggest that the hygienic condition of contact lens can vary by these factors influencing on disinfection efficacy and the occurrence of adverse effect can be affected.

• 제어로봇시스템학회:학술대회논문집
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• 1989.10a
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• pp.1005-1010
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• 1989

A refined version of automatic micro-organism recognition and identification method, 'O.I.S.M.2' is proposed in this paper, using image processing based on an expert system. This proposed method is based on the segmentation of the organism image, characterizing segment features, which are independent of individual size and length. Complicated shapes of organisms are divided into basic shape segments defined in this paper such as lines, circles, ovals etc. Organisms can then be expressed simply in a set of segments, regardless their individual differences.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.85-96
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• 2003

Normal human skin, constantly challenged by environmental micro-organisms, has an innate ability to fight invading microbes through antimicrobial peptides. These peptides, described in both plant and animal kingdoms are able to inactivate a broad spectrum of micro-organisms. Mammalian defensins constitute one of the most common antimicrobial peptide family. Among the three human beta-defensins hBD1, hBD2 and hBD3 produced in epithelia, only hBD2 and hBD3 are inducible and additionally have been described as expressed by differentiated keratinocytes at site of inflammation and infection. The aims of these studies were to define a cell culture model in which the basal production of hBD could be detected and up-regulated in order to enhance skin auto-protection against micro-organisms. A specific Polymerase Chain Reaction method have been developed for hBD2 and hBD3 mRNA detection in non-differentiated monolayer keratinocytes cell culture. We have been able to demonstrate that in vitro, hBD2 and hBD3 expression in normal human keratinocytes could be detected and enhanced by TNF-alpha and IFN-gamma, in hypercalcic culture conditions. This research opened the possibility of the development of cosmetic active compounds, able to induce the expression of skin natural antibiotic peptides responsible about microflora ecology of the skin.

• Korean Journal of Microbiology
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• v.17 no.3
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• pp.131-136
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• 1979

Forty-six Streptococci were isolated from human dental plaque. Each isolated from a different person, and their morphological and physiological characteristics investigated. It was found that the isolated micro-organisms 1. Most found that the isolated micro-organisms 2. Acid produced from maltose, inulin, manitol, sorbitol, lactose, mannose, 3. In the sucrose broth, most of them formed gelatinous clusters adhearing to the wall of the tube.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.757-760
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• 2008

Recently, improvements in the standard of living in industrial area require establishment of a convenient residential environment in order to enhance the quality of living, To achieve such an environment, it is necessary to effectively reduce or prevent various environmental problems occurring in and around residential areas. In this study, We focused on the water-purification properties of concrete using effective micro-organisms. Such as SS, BOD, COD, T-P, T-N. The ability of the removal of SS, T-P, T-N in the test water is superior to concrete using effective micro-organisms. As a result, concrete using effective micro-organisms has sufficient performance of water-purification.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.4
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• pp.375-379
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• 1996

The experiment investigated the possibility of using effluent from RUSITEC (rumen simulation technique) inoculated with rumen liquor or cow faeces as sources of micro-organisms for in vitro digestion of forages. Nine forages ${\times}3$ sources of inoculum were used in a factorial arrangement of treatments. Rumen liquor was collected from fistulated sheep and faeces was collected from cows. The RUSITEC apparatus consisted of 4 vessels, 2 vessels were charged with faecal liquor and 2 with rumen liquor. On the 8th day of the experiment RUSITEC effluent were collected to use in in vitro studies. In vitro OMD (g/kg) values using three sources of inoculum (fresh rumen liquor, RUSITEC effluent from rumen liquor or cow faeces) were statistically significant (p < 0.001). The regression relationships between OMD using fresh rumen liquor and RUSITEC effluent were highly significant ($R^2>0.90$). The results suggest that RUSITEC effluent either from rumen liquor or cow faeces can be used as a source of micro-organisms for in vitro digestion of forages.

• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.28-33
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• 1997

This study was carried out to investigate factors affecting anaerobic digestion enhancement of waste activated sludge(WAS). In order to this investigation, the degradability and rupture of microorganisms cell present in WAS, and mesophilic anaerobic digestion(MAD) of these compounds, were also evaluated. The micro-organisms cell in WAS were ruptured by a mechanical jet stream and smashed under pressure of 30 bar. The rupture level of micro-organisms cell in WAS were determined using phosphate, soluble protein and soluble chemical oxygen demand (SCOD)concentrations. It was found that the rupture level of micro- organisms cell within WAS increased with increasing pretreatment times, and the pretreated WAS once under pressure of 30 bar resulted in an increase in VS removal and methane production of 5%, 9% over the intact WAS of 35%, 71%, respectively, in batchwise MAD of 6-day and 14-day retention time. With the pretreatment and MAD of 6-day retention time used, mesophlic bioconvertibility as the biogasification of WAS were found to be significantly higher biogas of 1, 850 ml than 300 ml under intact WAS. In conclusion it can be stated mechanical pretreatment enhances WAS bioconvertibility, while under identical treatment conditions, resulted in a considerable decrease in the bioconvertibility of intact WAS.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.15 no.1
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• pp.47-54
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• 2011

In traditional Korean medicine, inspection of the tongue is an important method of making medical diagnoses and determining prognosis. We surveyed the fluorescence characteristics of the tongue coat in the ultraviolet light. The tongue coat comprises micro-organisms, blood metabolites, leukocytes from periodontal pockets, large amounts of desquamated epithelial cells released from the oral mucosa and different nutrients. In the ultraviolet light tissues of the oral cavity generally emit weak red or green fluorescence, which is not easily seen by the human eye, but is readily detected. This fluorescence has been proved to be due to the production of porphyrins by oral micro-organisms. While the composition of motile micro-organisms on the dorsum of the tongue is not constant, variations also occur persistingly in the fluorescence characteristics of the tongue coat. But because live bacteria contain a variety of intracellular biomolecules that have specific excitation and emission wavelength spectra characterizing their intrinsic fluorescence, the tongue coat emits fluorescence. the tongue itself, on the other hand, emits very weak or not fluorescence. In conclusion, we suggests that the uncoated tongue area be eliminated from the coated tongue area with the difference between the fluorescence characteristics of the tongue and that of the tongue coat.