• 대한치과보철학회지
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• 제48권4호
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• pp.301-307
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• 2010

연구 목적: 이번 연구의 목적은 법랑질 두께를 측정하는데 있어 microCT의 정확성을 평가하고, 한국인의 상악전치 법랑질 두께를 알아봄으로써 임상에 도움을 주고자 한다. 연구 재료 및 방법: 발거된 상악 중절치 5개를 레진 포매하여 치아 장축을 따라 정중부에서 협설로 절단하고 연마한 후에 microCT를 촬영하여 scanning electron microscope 상(${\times}20$)에서와 microCT 상에서 거의 동일한 절단면을 구현하였고, 각각 백악법랑경계 상방 1, 3, 5 mm 부위의 협측 법랑질 두께를 측정하여 microCT의 정확성을 평가하였다. 또한 상악 중절치 26개와 측절치 11개를 이용하여 각 치아의 순측 정중부와 정중부에서 근원심으로 각각 2 mm 떨어진 선상에서 백악법랑경계의 상방 1, 3, 5 mm 지점의 법랑질 두께를 측정하였다. 정중부의 세 지점에서는 정중부를 지나는 시상면에서 법랑질 표면에 접선을 그어 이 접선에 수직으로 법랑상아경계까지 수선을 내려 법랑질 두께를 측정하였고, 근원심 2 mm를 지나는 선상에 있는 6개 지점에서는 각 지점을 지나는 수평면에서 법랑질 표면에 접선을 그어 이 접선에 수직으로 법랑상아경계까지 수선을 내려 법랑질 두께를 측정하였다. 결과: 동일한 절단면에서 주사전자현미경과 microCT를 이용하여 백악법랑경계 상방 1, 3, 5 mm 지점의 순면 법랑질 두께를 측정한 값은 평균 3.81% 차이가 났으나, independent t-test 결과 두 가지 방법에 의한 측정값은 통계적으로 유의한 차이가 없었다. 상악 중절치의 정중부에서 백악법랑경계 1, 3, 5 mm 상방의 순면 법랑질 두께는 각각 $0.32{\pm}0.01$, $0.50{\pm}0.02$, $0.70{\pm}0.02\;mm$로 나타났다. 상악 측절치의 정중부에서 백악법랑경계 1, 3, 5 mm 상방의 순면 법랑질 두께는 각각 $0.30{\pm}0.01$, $0.55{\pm}0.03$, $0.80{\pm}0.02\;mm$로 나타났다. 결론: 법랑질 두께를 측정하는데 있어 microCT는 유용한 측정방법의 하나이며, 이번 연구의 결과로 볼 때 한국인의 상악전치부 도재 라미네이트 수복시 법랑질의 삭제량에 주의를 기울일 필요가 있는 것으로 사료된다.

• Imaging Science in Dentistry
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• 제35권3호
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• pp.167-173
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• 2005

Purpose : To investigate the relationship between three-dimensional (3D) bone imaging parameters and trabecular strength in the mandible. Materials and Methods : Bone specimens were obtained from the mandibles of five male pigs weighing around 110 kg each. Of those, 43 samples were selected for 3D analysis and measured by micro-computed tomography. The five morphometric parameters were trabecular thickness (Tb.Th), bone specific surface (BS/BV), percent bone volume (BV/TV), structure model index (SMI) and degree of anisotropy (DA). Through destructive mechanical testing, strength parameters were obtained. Results : BV/TV, SMI, BS/BV, and Tb.Th showed significant correlations with strength parameters. DA did not show any correlation with the other parameters. In multiple linear regression analysis, BV/TV alone explained $43\%$ of the variance in Young's modulus. By stepwise inclusion of SMI, the variance in the Young's modulus was better explained up to $52\%$. Conclusions : Predicting trabecular strength in the mandible through architectural analysis would be possible. Further study is needed to establish the tendency and variety of trabecular architecture and strength according to the locations within the mandible.

• Restorative Dentistry and Endodontics
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• 제45권2호
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• pp.4.1-4.6
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• 2020

Although numerous studies have been conducted on apexification using mineral trioxide aggregate (MTA), direct observation of extracted human teeth after the procedure has been rarely reported. This case report describes a mandibular premolar treated 2.5 years ago and extracted recently for orthodontic treatment. The tubercle of the right mandibular premolar of a 12-year-old boy with dens evaginatus was fractured and the pulp was exposed. The tooth was diagnosed with pulp necrosis and asymptomatic periapical abscess. During the first visit, copious irrigation was performed with 2.5% sodium hypochlorite. Calcium hydroxide paste was placed as an intracanal medicament. The sinus tract had disappeared at the second visit after 3 weeks. MTA was applied on to the bleeding point as a 4-mm-thick layer, followed by a 3-mm-thick gutta-percha filling and resin core build-up. After 2.5 years, the tooth and three other premolars were extracted for orthodontic treatment. The right and left mandibular premolars were scanned with micro-computed tomography to determine the root shape and canal anatomy. Irregular root growth was observed and the root outline of the right mandibular premolar differed from that of the contralateral tooth. Apexification with MTA leads to the formation of roots with irregular morphology, without any pulpal space.

• Journal of Periodontal and Implant Science
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• 제41권5호
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• pp.218-226
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• 2011

Purpose: Micro-computed tomography (micro-CT) has been widely used in the evaluation of regenerated bone tissue but the reliability of micro-CT has not yet been established. This study evaluated the correlation between histomorphometric analysis and micro-CT analysis in performing new bone formation measurement. Methods: Critical-size calvarial defects were created using a 8 mm trephine bur in a total of 24 Sprague-Dawley rats, and collagen gel mixed with autogenous rat bone marrow stromal cells (BMSCs) or autogenous rat BMSCs transduced by adenovirus containing bone morphogenic protein-2 (BMP-2) genes was loaded into the defect site. In the control group, collagen gel alone was loaded into the defect. After 2 and 4 weeks, the animals were euthanized and calvaria containing defects were harvested. Micro-CT analysis and histomorphometric analysis of each sample were accomplished and the statistical evaluation about the correlation between both analyses was performed. Results: New bone formation of the BMP-2 group was greater than that of the other groups at 2 and 4 weeks in both histomorphometric analysis and micro-CT analysis (P=0.026, P=0.034). Histomorphometric analysis of representative sections showed similar results to histomorphometric analysis with a mean value of 3 sections. Measurement of new bone formation was highly correlated between histomorphometric analysis and micro-CT analysis, especially at the low lower threshold level at 2 weeks (adjusted $r^2=0.907$, P<0.001). New bone formation of the BMP-2 group analyzed by micro-CT tended to decline sharply with an increasing lower threshold level, and it was statistically significant (P<0.001). Conclusions: Both histomorphometric analysis and micro-CT analysis were valid methods for measurement of the new bone in rat calvarial defects and the ability to detect the new bone in micro-CT analysis was highly influenced by the threshold level in the BMP-2 group at early stage.

• 대한치과의사협회지
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• 제54권11호
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• pp.865-873
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• 2016

Background/Purpose: The purpose of this study was to evaluate the effect of Nd:YAG irradiation on adherence of retrograde filling materials (mineral trioxide aggregate [MTA] and Super-EBA) by micro-computed tomography (CT) measurement and to observe the dentinal surface after irradiation by scanning electron microscopy (SEM). Materials and methods: Forty retrofilling models using extracted human teeth were divided into four groups according to the material and method used: ProRoot MTA (MTA group), Super-EBA (EBA group), MTA with Nd:YAG laser irradiation (LMTA group), and Super-EBA with Nd:YAG laser irradiation (LEBA group). All specimens were stored in 100% humidity for 24 hours until micro-CT was performed. The gap volume of the tooth/material interface was measured using the CTAn program. In six samples, the laser-irradiated dentin surface was observed using SEM. Results: The mean percent difference in gap volume was not statistically significant between the Nd:YAG laser-irradiated groups and non-irradiated in both materials(P > 0.05). The gap volume in the MTA group was significantly lower than that in the EBA group (P < 0.05). Examination of the non-irradiated specimens by SEM showed patent dentinal tubules. In contrast, alterations in the texture of the dentin surface and obliteration of the dentinal tubules were evident in the Nd:YAG laser-irradiated specimens. Conclusion: In this study, changes in the dentinal surface after Nd:YAG irradiation did not affect adherence between the apical filling material and the dentin wall.

• Journal of Korean Neurosurgical Society
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• 제54권6호
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• pp.461-466
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• 2013

Objective : Although curcumin has a protective effect on bone remodeling, appropriate therapeutic concentrations of curcumin are not well known as therapeutic drugs for osteoporosis. The purpose of this study was to compare the bone sparing effect of treatment of low-dose and high-dose curcumin after ovariectomy in rats. Methods : Forty female Sprague-Dawley rats underwent either a sham operation (the sham group) or bilateral ovariectomy (OVX). The ovariectomized animals were randomly distributed among three groups; untreated OVX group, low-dose (10 mg/kg) curcumin administered group, and high-dose (50 mg/kg) curcumin group. At 4 and 8 weeks after surgery, serum biochemical markers of bone turnover were analyzed. Bone histomorphometric parameters of the 4th lumbar vertebrae were determined by micro-computed tomography (CT). In addition, mechanical strength was determined by a three-point bending test. Results : High-dose curcumin group showed significantly lower osteocalcin, alkaline phosphatase, and the telopeptide fragment of type I collagen C-terminus concentration at 4 and 8 weeks compared with the untreated OVX group as well as low-dose curcumin group. In the analyses of micro-CT scans of 4th lumbar vertebrae, the high-dose curcumin treated group showed a significant increase in bone mineral densities (p=0.028) and cortical bone mineral densities (p=0.036) compared with the low-dose curcumin treated group. Only high-dose curcumin treated group had a significant increase of mechanical strength compared with the untreated OVX group (p=0.015). Conclusion : The present study results demonstrat that a high-dose curcumin has therapeutic advantages over a low-dose curcumin of an antiresorptive effect on bone remodeling and improving bone mechanical strength.

• Journal of Oral Medicine and Pain
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• 제45권4호
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• pp.83-88
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• 2020

Purpose: Growth differentiation factor 11 (GDF11) and myostatin (MSTN) are closely-related transforming growth factor β family members reported to play crucial roles in bone formation. We previously reported that, in contrast to MSTN, GDF11 promotes osteogenesis of vertebrae and limbs. GDF11 has been also reported as an important regulator in tooth development by inducing differentiation of pulp stem cells into odontoblasts for reparative dentin formation. The goal of this study was to investigate the differential roles of GDF11 and MSTN in dental and cranial bone formation. Methods: Micro-computed tomography analysis was performed on cranial bones, including frontal, parietal, and interparietal bones, and lower incisors of wild-type, Gdf11 knockout (Gdf11-/-), and Mstn knockout (Mstn-/-) mice. Tissue volume, thickness, and mineral density were evaluated for both cranial bone and lower incisors. Lower incisor lengths were also measured. Because Gdf11-/- mice die shortly after birth, analysis was performed on newborn (P0) mice. Results: Compared to those of Mstn-/- mice, cranial bone volume, thickness, and mineral density levels were all significantly diminished in Gdf11-/- mice. Tissue mineral density of Gdf11-/- mice were also significantly decreased compared to wild-type mice. Likewise, lower incisor length, tissue volume, thickness, and mineral density levels were all significantly reduced in Gdf11-/- mice compared to Mstn-/- mice. Incisor length was also significantly decreased in Gdf11-/- mice compared to wild-type mice. Mstn-/- mice exhibited mildly increased levels of tissue volume, thickness, and density in cranial bone and lower incisor compared to wild-type mice although statistically not significant. Conclusions: Our findings suggest that GDF11, unlike MSTN, endogenously promotes cranial bone and tooth development.

• 대한치과교정학회지
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• 제53권3호
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• pp.205-216
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• 2023

Objective: To investigate changes in the immature teeth of Sprague-Dawley rats during orthodontic treatment and to explore the changes in the peri-radicular alveolar bone through micro-computed tomography (CT). Methods: Twenty-five 26-day-old male Sprague-Dawley rats were included. The maxillary left first molar was moved mesially under a continuous force of 30 cN, and the right first molar served as the control. After orthodontic treatment for 7, 14, 21, 28, and 42 days, the root length, tooth volume, and alveolar bone mineral density (BMD) around the mesial root were measured through micro-CT. Results: The immature teeth continued to elongate after application of orthodontic force. The root length on the force side was significantly smaller than that on the control side, whereas the differences in the volume change between both sides were not statistically significant. Alveolar bone in the coronal part of the compression and tension sides showed no difference in BMD between the experimental and control groups. The BMD of the experimental group decreased from day 14 to day 42 in the apical part of the compression side and increased from day 7 to day 42 in the apical part of the tension side. The BMD of the experimental group decreased in the root apex part on day 7. Conclusions: The root length and volume of immature teeth showed continued development under orthodontic forces. Alveolar bone resorption was observed on the compression side, and bone formation was observed on the tension side.

• Restorative Dentistry and Endodontics
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• 제47권1호
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• pp.6.1-6.9
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• 2022

Objectives: This study investigated the internal morphology of mesiobuccal (MB) roots of maxillary molars with a second mesiobuccal (MB2) canal. Materials and Methods: Forty-seven maxillary first or second molars from Brazilians were scanned using micro-computed tomography. The following measurements were obtained from the MB roots: root thickness, root width, and dentin thickness of the buccal aspect of the first mesiobuccal (MB1) canal, between the MB1 and MB2 canals, and the palatal aspect of the MB2 and MB1 canals at 3 mm from the root apex and in the furcation region. For statistical analysis, the Student's t-test and analysis of variance with the post-hoc Tukey test were used (α = 0.05). Results: In maxillary molars with an MB2 canal, MB roots were significantly thicker (p = 0.0014) and narrower (p = 0.0016) than in maxillary molars without an MB2 canal. The dentin thickness of the palatal aspect of the MB1 canal was also significantly greater than that of MB roots without an MB2 canal at 3 mm from the root apex (p = 0.0007) and in the furcation region (p < 0.0001). In the furcation region of maxillary molars with an MB2 canal, the dentin thickness between the MB1 and MB2 canals was significantly smaller than that in the buccal and palatal aspects (p < 0.0001). Conclusions: The internal morphology of MB roots of maxillary molars with an MB2 canal revealed differences in dentin thickness, root diameter, and distance between the canals when compared with maxillary molars without an MB2 canal.

• Restorative Dentistry and Endodontics
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• 제32권1호
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• pp.61-68
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• 2007

이 연구의 목적은 네 종류 file systems의 중심유지능과 근관성형 전후의 상아질 삭제량을 비교하는 것이다. 10-20도의 만곡을 갖는 발거된 20개의 하악 제1대구치의 근심근관(총 40개의 근관)을 근관성형 전에 X선 미세단층촬영 스캐너를 이용하여 스캔하였다. 제 1군은 근관부를 넓힌 후 stainless steel K-Flexofile을 사용하여 step-back technique으로 근관성형하였고, 나머지 군들은 각 제조사의 추천대로 ProFile system (2군), ProTaper system (3군), K3 system (4군)을 사용하여 crown-down technique으로 근관성형하였다. 모든 근관의 근단부 기구조작은 #25 크기까지 시행하였고 근관성형 후 스캔하였다. 3차원 영상 소프트웨어로 근관성형 전후의 스캔된 이미지들을 재구성하여 근관의 전체적 부피 변화를 측정하였다. 또한, 근단공으로부티 1, 3, 5, 7 mm되는 지점의 근관 횡단면을 비교하여 근관성형 전후의 단면적 변화와 중심변위율을 산출하였다. 그 결과, ProTaper와 K3가 다른 file systems보다 상아질을 더 많이 삭제하는 경향을 보였고 모든 실험군에서 중심 변위율은 근단공으로부터 3 mm 지점에서 가장 낮은 수치를 나타냈으며, 3 mm 지점을 제외하고는 ProTaper가 다른 file systems보다 중심유지능이 떨어지는 결과를 얻을 수 있었다 (P < 0.05).