• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.6
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• pp.133-144
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• 2021

This study aims to estimate location of land mammals habitat by analyzing spatial data and investigate how to apply environmental DNA monitoring methodology to lotic system in Yangpyeong-gun, Gyeonggi-do. Environmental DNA sampling points are selected through spatial analysis with QGIS open source program by overlaying Kernel density of wild boar(Sus scrofa), elevation, slope and land-cover map, and 81 samples are collected. After 240 mL of water was filtered in each sample, metabarcoding technique using MiMammal universal primer was applied in order to get a whole list of mammal species whose DNA particles contained in filtered water. 8 and 22 samples showed DNA of wild boar and water deer, respectively. DNA of raccoon dog, Eurasian otter, and Siberian weasel are also detected through metabarcoding analysis. This study is valuable that conducted in outdoor lotic system. The study suggests a new wildlife monitoring methodology integrating overlayed geographic data and environmental DNA.

• Journal of Korean Society of Rural Planning
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• v.15 no.2
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• pp.19-26
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• 2009

The purpose of this study is to build Island biogeography in the basic concept of landscape ecology in South Korea by draw relationship between the species side of quantitative habitats and forest area surveyed in the national database based on investigation of the 2nd natural environment. In addition, try to present criterion of habitats character category after understanding habitats character of emergence area side of quality habitats based on the type of formatting. Species and forest area relationship analyzed using correlation analysis and simple regression analysis. Also habitat character limited composition ratio of neighboring land cover and analyzed using hierarchical cluster analysis to classify type of habitat. As a result, we found that forest area is correlated with number of species, forests which is bigger than 100ha are more important of increase in species' population. And according to land cover composition ratio, bird's classified types of forest inner species, forest edge species, forest outer species and mammal's classified types of forest inner species, forest general species, forest edge species. We suggest that study of species-forest area relationship and emergence habitat character be used as some management plans of species' conservation, protection and restoration.

• Animal Systematics, Evolution and Diversity
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• no.spc9
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• pp.73-79
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• 2016

In the present study, to explore the current status of mammals that inhabit Jindo Island, Jeollanam-do, South Korea, a survey was conducted from July 6 to July 9, 2016. Using several survey methods, such as Sherman's live trap, footprint, caves, excretions, and road-kill investigations, the mammals observed on Jindo Island were found to be from 5 orders and 9 families, including 12 species in total. Among them, three species of small mammals were Mogera wogura, Crocidura shantungensis, and Apodemus agrarius. Additionally, two legally protected species were found through excretion observations. Lutra lutra and Prionailurus bengalensis, which were designated to endangered wild animal classes I and II, respectively. The survey results can be used as a valuable resource for identifying the distribution and habitat status of mammals in the Jindo-gun area, as well as for building a database for ecosystem preservation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.2
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• pp.69-89
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• 2020

Scientific trust and quantification of traditional species investigation and results that have been used in ecology for decades has always been a problem and concern for ecologists. Global ecologists have proposed DNA-based species investigation studies to find answers to problems. In this study, we reviewed the global trend of research on environmental DNA(eDNA), which is a method for monitoring species by detecting DNA of organisms naturally mixed in environmental samples such as water, soil, and feces. The first eDNA research confirmed the possibility of species investigation at the molecular level, and commercialization of NGS(Next Generation Sequencing) and DNA metabarcoding elicits efficient and quantitative species investigation results, and eDNA research is increasing in the filed of ecology. In this study, mammals and birds were detected using MiMammal universal primers from 23 samples(3 natural reserves; 20 water bowls) out of 4 patches to verify eDNA for urban ecosystems in Suwon, and eDNA was verified by performing camera trapping and field survey. Most terrestrial species were detected through eDNA, and particularly, mice(Mus musculus), and Vinous-throated Parrotbill (Sinosuthora webbiana) were identified only with eDNA, It has been confirmed to be highly effective by investigating techniques for small and internal species. However, due to the lack of resolution of the primer, weasels(Mustela sibirica) and squirrels(Melanochromis auratus) were not detected, and it was confirmed that the traditional investigation method was effective only for a few species, such as Mogera robusta(Mogera robusta). Therefore, it is judged that the effects of species investigation can be maximized only when eDNA is combined with traditional field survey and Camera trapping to complement each other.

• Journal of Environmental Impact Assessment
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• v.32 no.2
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• pp.94-111
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• 2023

In order to establish an effective management strategy for invasive species early detection and regular monitoring are required to assess their introduction or dispersal. Environmental DNA (eDNA) is actively applied to evaluate the fauna including the presence of invasive species as it has high detection sensitivity and can detect multiple species simultaneously. In Korea, the applicability evaluation of metabarcoding is being conducted mainly on fish, and research on other taxa is insufficient. Therefore, this study identified the feasibility of detecting invasive species in Korea using eDNA metabarcoding. In addition, to confirm the possibility of detection by taxa, the detection of target species was evaluated using four universal primers (MiFish, MiMammal, Mibird, Amp16S) designed for fish, mammals, birds, and amphibians. As a result, target species (Trachemys scripta, 3 sites; Cervus nippon, 3 sites; Micropterus salmoides, 7 sites; Rana catesbeiana, 4 sites) were detected in 17 of the total 55 sites. Even in the selection of dense sampling sites within the study area, there was a difference in the detection result by reflecting the ecological characteristics of the target species. A comparison of community structures (species richness, abundance and diversity) based on the presence of invasive species focused on M.salmoides and T.scripta, showed higher diversity at the point where invasive species were detected. Also, 1 to 4 more species were detected and abundance was also up to 1.7 times higher. The results of invasive species detection through metabarcoding and the comparison of community structures indicate that the accumulation of large amounts of monitoring data through eDNA can be efficiently utilized for multidimensional ecosystem evaluation. In addition, it suggested that eDNA can be used as major data for evaluation and prediction, such as tracking biological changes caused by artificial and natural factors and environmental impact assessment.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.4
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• pp.43-56
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• 2014

In this study, ecological investigation was performed on Yong-neup, Daeam-san for 5 times. This area is considered DMZ zone located between Yanggu-gun and Inje-gun, Gangwon-do, where has been recognized highly valuable for ecological preservation. As a result, Sphagnum and hydrophobic vegetation were found in Little Yong-neup, revealing its characteristics of high moor, where as no Sphagnum were found in Baby Yong-neup. Thereby, the carnification has been investigated in both Little Yong-neup and Baby Yong-neup. A main cause of damage was associated with the creation of stating rink and increases of visitors. The damages of vegetation in Little Yong-neup and Baby Yong-neup have been intensified by road construction or uses of groundwater while relocating military troops to upper stream of Little Yong-neup and Baby Yong-neup, further causing the damages of water circulation system and soil erosion. These artificial factors have caused the carnification of wetland protection areas including Little Yong-neup. The terrestrial vegetations, such as Plantago asiatica, Geranium Koreanum and Artemisia feddei have increased compared to current survey conducted by the Ministry of Environment. 5 endangered species, such as Lychnis wilfordii and Trientalis europaea and 5 other introduced species, such as Aster pilosus and Taraxacum officinale were found. 10 different species of mammal and 2 species of amphibian were investigated; and in addition to this, a original form of Sphagnum fens, the remaining wetland was found. Therefore, this study is to identify the causes of damages in Little Yong-neup and Baby Yong-neup through their ecological survey and accordingly proposing a direction for ecological restoration through the improvement of water circulation system, creation of habitats for plant and animal, restoration of vegetation through eco-friendly materials and indigeneity, relocation of the current military troops, securing of structural stability.

• Animal cells and systems
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• v.16 no.3
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• pp.230-236
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• 2012

The Korean hare, Lepus coreanus, is an important mammal in ecosystem food chains, and is distributed across the entire Korean peninsula and northeastern China. Polymorphic microsatellite loci were developed using the biotinenrichment technique for use in population genetics studies. Five trinucleotide and four dinucleotide microsatellite loci were selected and tested on 22 Korean hare specimens collected from Gangwon Province and Gyeongsangbuk Province in South Korea. The number of alleles across the two sampling regions ranged from three to nine with a mean of 6.1. Mean observed and expected heterozygosities and polymorphic information content were 0.540, 0.627 and 0.579, respectively. Only one locus, Lc06, showed departure from Hardy-Weinberg equilibrium after applying the Bonferroni correction. Four microsatellites, Lc01, Lc03, Lc12, and Lc19, satisfied the criteria to serve as a core set of markers recommended for population genetics studies. These new microsatellite markers will be widely applicable to future genetic studies for management and conservation of the Korean hare and related species, including assessment of the genetic diversity and population structure of L. coreanus.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.259-264
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• 2001

Toxicological studies of two potential biological control agents, the entomopathogenic nematode (Steinernema carpocapsae) and the symbiotic bacteria (Xenorhabdus nematophilus) were conducted against two beneficial insects and one mammal species. Two microbial agents varied in their toxicities between two insect species: an ant, Pristomyrmex pungens, and silkworm, Bombyx mori. In oral toxicity test, the symbiotic bacteria resulted in significant lethal [half lethal concentration of $1.4$\times$10^3$colony-forming units (cfu)/ml] on the ants, while they gave little lethal effect (half lethal concentration of more than $10^{8}$ cfu/ml) on the silkworms. The nematodes, however, gave significant lethal effect [half lethal concentration of 4 infected juveniles (IJs)/ml] on the silkworms, while they did little lethal effect (half lethal concentration of 150,000 IJs/ml) on the ants in topical assays. Both the nematodes and the bacteria did not give lethal effect to the albino rats, Rattus norvegicus, when they were fed orally into the rats. Also, any of these microbial agents were not detected in the internal organs of the treated rats.