Journal of the Korean Society of Environmental Restoration Technology (한국환경복원기술학회지)

The Korea Society of Environmental Restoration Technology (한국환경복원기술학회)
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Monitoring the presence of wild boar and land mammals using environmental DNA metabarcoding - Case study in Yangpyeong-gun, Gyeonggi-do -

환경 DNA 메타바코딩을 활용한 멧돼지 및 육상 포유류 출현 모니터링 - 경기도 양평군 일대를 중심으로 -

  • Kim, Yong-Hwan (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Han, Youn-Ha (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Park, Ji-Yun (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University) ;
  • Kim, Ho Gul (Dept. of Human Environment Design, Major in Landscape Urban Planning, Cheongju University) ;
  • Cho, Soo-Hyun (Plant Resources Division, National Institute of Biological Resources) ;
  • Song, Young-Keun (Dept. of Landscape Architecture, Graduate School of Environmental Studies, Seoul National University)
  • 김용환 (서울대학교 환경대학원 환경조경학과) ;
  • 한윤하 (서울대학교 환경대학원 환경조경학과) ;
  • 박지윤 (서울대학교 환경대학원 환경조경학과) ;
  • 김호걸 (청주대학교 휴먼환경디자인학부 조경도시계획전공) ;
  • 조수현 (국립생물자원관 식물자원과) ;
  • 송영근 (서울대학교 환경대학원 환경조경학과)
  • Received : 2021.11.14
  • Accepted : 2021.12.19
  • Published : 2021.12.31
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Abstract

This study aims to estimate location of land mammals habitat by analyzing spatial data and investigate how to apply environmental DNA monitoring methodology to lotic system in Yangpyeong-gun, Gyeonggi-do. Environmental DNA sampling points are selected through spatial analysis with QGIS open source program by overlaying Kernel density of wild boar(Sus scrofa), elevation, slope and land-cover map, and 81 samples are collected. After 240 mL of water was filtered in each sample, metabarcoding technique using MiMammal universal primer was applied in order to get a whole list of mammal species whose DNA particles contained in filtered water. 8 and 22 samples showed DNA of wild boar and water deer, respectively. DNA of raccoon dog, Eurasian otter, and Siberian weasel are also detected through metabarcoding analysis. This study is valuable that conducted in outdoor lotic system. The study suggests a new wildlife monitoring methodology integrating overlayed geographic data and environmental DNA.

Keywords

Acknowledgement

본 논문은 정부(환경부)의 재원으로 국립생물자원관의 지원을 받아 수행하였습니다(NIBR202105102).

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