• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.313-321
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• 2021

BACKGROUND: Ammonia is a causative substance for the fine particulate matters (PM_2.5) and generates dust through atmospheric reactions. Agricultural sector accounts for 79.3% of ammonia emissions in Korea. Urea and composted organic fertilizer (COF) are used in the soil for the purpose of supplying nutrients in grapevine orchards. This study was conducted to investigate estimates of ammonia emission and examine fruit quality from the rain proof cultivation of the 'Beni Balad', applied by urea and COF to the soil. METHODS AND RESULTS: Urea, COF1, and COF2 were applied at the rates of 119, 135, and 271 kg ha^-1 respectively. Ammonia emissionwas measured using a dynamic flow-through method. CONCLUSION(S): Ammonia emissions by urea and COF treatments to 'Beni Balad' soils under rain proof cultivation were calculated to be 2.63, 12.95, 2.05, and 3.97 kg NH₃-N ha^-1 day^-1, respectively for the control, urea, COF1, and COF2. Urea soil application increased soluble solids, firmness, and anthocyanin content in fruits at harvest, and COF1 application increased the soluble solids and anthocyanin content. For all the treatments, acidity increased in the harvested fruits.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.38-47
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• 1988

To determine the response of soybean cultivars to nitrogen in relation to acidity, nodulation, content of allantoin nitrogen and major chemical compositions of soybean plants were investigated with two cultivars under two levels of soil pH and four levels of nitrogen fertilization in a field and nutri-culture experiments. Nodulation and contents of allantoin nitrogen, total nitrogen, potassium, calcium, magnesium and manganese of soybean plants decreased under acidic condition, but the degree of decrease was smaller in the variety Jangbaegkong compared to the variety Danyeobkong. On the other hand, nodulation, content of allantoin nitrogen and potassium decreased with increased nitrogen fertilization, particulary under pH 7 condition. Nodulation was positively correlated with the content of allantoin nitrogen of soybean plants. The content of total nitrogen of soybean plants increased with increased nitrogen fertilization, and this tendency was remarkable under acidic condition and in the variety Jangbaegkong.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.151-151
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• 2023

본 연구는 호우(heavy rain) 발생 시 대기 중 미세먼지(particulate matter, PM) 저감효과를 규명하고 강우 지속에 따른 빗물 수질(pH, 전기전도도(electrical conductivity, EC), 수용성 이온) 분석을 통해 대기 중 PM이 빗물 수질에 미치는 영향을 평가하였다. 2020년 3월부터 2021년 2월까지강우 강도(7.5 mm/h)를 기준으로 총 6회의 강우를 대상으로 하였으며 빗물 샘플은 집수장치를 통해 50 mL를 연속적으로 수집하여 수질을 분석하였다. 대기 중 PM_2.5 (≤ 2.5 ㎛ in diameter) 및 PM₁₀ (≤ 10 ㎛ in diameter) 농도는 기상청 내 부산 남구 대연동 관측소의 automatic weather system (AWS)에서 측정된 일평균 자료를 이용하였다. 강우에 따른 대기 중 PM의 저감효율은 상대적으로 PM₁₀에서 뚜렷하게 나타났으며, 특히 강우 강도 7.5 mm/h 이상(유형 1)의 호우 발생 시60% 이상의 저감효율을 보였다. 반면, 강우 강도 7.5 mm/h 이하(유형 2)일 때는 10% 이하의 저감효율을 보였으며, 강우 지속에 따라 대기 중 PM₁₀ 농도가 증가하는 경향을 보이기도 하였다. 총108개의 빗물 샘플 수질을 분석한 결과, 유형 1의 경우 초기 빗물의 평균 EC는 58.5 µS/cm으로 상대적으로 높았으며 대기 중 PM₁₀과 양의 상관관계(r = 0.99)를 보였고 평균 pH는 4.3으로 산성도가 높게 나타났으며 대기 중 PM₁₀과 음의 상관관계(r = -0.99)를 보였다. 반면, 유형 2의 경우 대기 중 PM₁₀과 EC (r = -0.56) 및 pH (r = -0.41) 간 뚜렷한 상관관계가 나타나지 않았다. 또한 강우가 지속됨에 따라 EC와 수용성 양이온(Na⁺, Mg²⁺, K⁺, Ca²⁺, NH₄⁺) 및 음이온(Cl^-, NO₃^-, SO₄^2-)의 농도는 지속적으로 감소하는 경향을 보였으나 pH의 경우 강우 강도에 따라 증감의 경향이 다르게 나타났다. 유형 1의 경우 강우 지속에 따라 pH가 증가하여 산성도가 낮아졌으나 유형 2는 pH의 증감 형태를 뚜렷하게 확인하기 어려웠다. 연구 결과를 통해 강우 초기 높은 강도로 강우가 지속될 경우 대기 중 PM₁₀이 빗물 수질에 영향을 미칠 수 있는 것으로 판단되며, 이에 따라 호우 발생 시 강우가 대기 중 오염물질을 지표면으로 유입시킬 수 있는 매개체로 작용할 수 있음을 지시한다.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.62-62
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• 2003

This study was carried out to investigate the effects of activation agents on parthenogenetic activation of pig oocytes matured in vitro. The medium used for oocyte maturation was tissue culture medium (TCM) 199 supplemented with 26.19 mM sodium bicarbonate, 0.9 mM sodium pyruvate, 10 $\mu\textrm{g}$/ml insulin, 2 $\mu\textrm{g}$/ml vitamin $B_{l2}$, 25 mM Hepes, 10 $\mu\textrm{g}$/ml bovine apotransferrin, 150 $\mu$M cysteamine, 10 IU/ml PMSG, 10 IU/ml hCG, 10 ng/ml EGF, 0.4% BSA, 75 $\mu\textrm{g}$/ml sodium penicillin G, 50 $\mu\textrm{g}$/ml streptomycin sulfate and 10% pFF. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5$^{\circ}C$, 5% $CO_2$ in air. Cumulus-free oocytes involving first polar body were activated by exposure to various concentrations of ethanol and exposure time of ethanol in Hepes-buffered NCSU23 medium. Also, oocytes were activated by electric pulse alone or combination with ethanol. For electrical activation, oocytes were rinsed twice in 0.3 M mannitol solution supplemented with 0.1 mM CaC1$_2$, 0.2 mM MgC1$_2$, 0.5 mM Hopes and 0.01% BSA, and transferred to a chamber consisting of two electrodes 1 mm apart which was overlaid with the same activation solution. Oocytes were activated with a single DC pulse of 1.3 ㎸/cm for 30 $\mu$sec. After activation treatments, oocytes were washed three times with Hepes-buffered NCSU23 medium and were washed twice with NCSU23 culture medium containing 0.4% BSA, and then cultured in 500 ${mu}ell$ of the same medium for 20 h at 38.5$^{\circ}C$, 5% $CO_2$ in air. The activation rates of oocytes were higher in 6, 7 and 8% ethanol concentrations compared with 0, 5, 9 and 10% ethanol concentrations. Significantly more oocytes (29.3~33.7%) were activated in the exposure for 8, 10, 12 and 15 min than those in the exposure for 0 and 5 min, but there was no difference due to exposure to 8% ethanol for 8 to 15 min. Electric pulse treatment followed by exposure to ethanol significantly improved the rate of oocyte activation (61.9%) compared with that of other 3 treatments. In conclusion, the optimal activation treatment of ethanol exposure alone for the in-vitro matured pig oocytes was 8% ethanol for 8 to 15 min. Electric pulse treatment followed by ethanol exposure significantly improved the rate of activation.n.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.21 no.1
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• pp.43-51
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• 1976

Marine deposite soil was studied to evaluate morohological changes of paddy rice under subdrainage control. The content of organic and inorganic mate rial were low at high temperature in the mid-summer. This soil lacked air and accumulated more H$_2$ S. Thus rice root was rotted with high infection of virus and insect and yield was relatively low.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.411-419
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• 1996

The experiment was aimed to determine a change of chemical component in irrigatted water based on different tillage methods and nitrogen rates under mulching of barley straw in direct seeded rice. There was no difference in water pH of no-tillaged plot but high in tillaged plot until 10 days after treatment. The electric conductivity(EC) of the water was higher in no-tillaged plot than in tillaged plot. However, the dissolved oxygen content was vice versa. The content of NH$_4$-N was high in higher application rate of N fertilizer without the tillage. Mean while, NO$_3$-N content was highly affected by no-till aged plot particularly in between application time and fertilizer rate but not in tillaged plot. There was higher in P043- content with the no-tillaged plot compared to the tillaged plot. It was big difference with higher application rate of the fertilizer. Soil cations were high in much application of fertilizer without the tillage.

• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.80-87
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• 2022

We investigated the effects of organic acid and ultrasonication treatment in maintaining the quality of garlic during storage. Samples were exposed for 5 min to either ultrasonication at 60℃, 1% citric acid, or 0.5% fumaric acid. Presence of microorganisms and minerals, hardness, and color were compared during storage at 4℃ for 28 days. The total aerobic bacterial count remained low. No proliferation of Escherichia coli was observed after treatment with fumaric acid or ultrasonication, and mold proliferation was inhibited by ultrasonication. The mineral content of the northern type garlic was higher than that of the southern type. Exposure to fumaric acid did not result in a substantial difference in hardness until 21 days of storage, at which time there was a decrease in the L-value in each cultivation type. Our results indicate that treatment with 0.5% fumaric acid for 5 min was effective in reducing the abundance of microorganisms during storage without affecting the hardness or color in garlic.

• Journal of Veterinary Science
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• v.23 no.1
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• pp.16.1-16.13
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• 2022

Background: Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown. Objectives: This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl. Methods: Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 ㎍/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated. Results: Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro. Conclusions: This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.310-317
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• 2022

BACKGROUND: The salt in soil interrupts crop growth. Therefore, water resources are used to remove any salt found in the soil. However, water resources have been reduced by global warming; thus, a new study is required into reducing the salt in soil. Recently, the bottom ash (BA) of a biomass power plant was found to be similar to biochar. Hence, it can be used to remove heavy metals and wastewater through the adsorption characteristics of BA. The objective of this study was to evaluate the improvement effects on crop growth in saline soil containing the BA from biomass power plants. METHODS AND RESULTS: The effect on crop growth in the saline soil supplemented with BA was studied with the crop-planted pots, which were packed by reclaimed greenhouse soils collected from Byolyang, Suncheon. The BA application level was 25, 50, 100, 200, and 400 kg/10a (referred as BA25, BA50, BA100, BA200, and BA400, respectively). The BA increased the fresh weights of the leaf and root, while nitrogen uptake increased by approximately 24-102% and 54-77%, respectively for the lead and root. The phosphorous uptake increased by 38%, although only in the leaf of the lettuce. In the case of soil, BA increased water content, pH, EC, CEC, and NH₄⁺ and the SAR of the soil decreased by 5-15%. The bottom ash increased the contents of Ca²⁺ and Mg²⁺, and fixed the amount of Na⁺. CONCLUSION(S): It was confirmed the bottom ash of a biomass power plant, based on wood pellets, improved crop growth, and increased the nutrient uptake of crops in saline soil. In addition, bottom ash, which has a wide range of porosity and high values of pH and EC, improved properties of the saline soil. However, the BA has a large amount of B, As, and heavy metals. Finally, it may require a study on the safety and contamination of heavy metals contained in the bottom ash, which would be applied in soil for a long time.

• Analytical Science and Technology
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• v.20 no.2
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• pp.147-154
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• 2007

Sweat contents were investigated for using those data to forensic purpose. The experiments of identifying sweat contents were as follow: 1) measurement of amino acids (aspartic acid, serine, glycine etc) by HPLC, 2) anions ($Cl^-$, $F^-$, and $SO{_4}^{2-}$) by IC and 3) trace elements (Cu, Zn, Li, B, etc.) by ICP-MS. Amino acid contents in sweat are varied with individual samples and glycine, threonine, alanine, valine and histidine are detected as the prime one. The detected anions are $Cl^-$(2167~4073 ppm) and $F^-$(454~582 ppm) mostly. Trace elements of Rb, Zn and Cu are detected and those concentrations are relatively very high. The compositions of sweat can be influenced by various factors (diet, anthropometric, characteristics, physical fitness, age, gender and the state of the health).