• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2005.05a
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• pp.237-240
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• 2005

The demand of micro electrical mechanical system (MEMS) bio/chemical sensor is rapidly increasing. To prevent the contamination of sensing area, a filtration system is required in on-chip total analyzing MEMS bio/chemical sensor. A nano-filter was mainly applied in some application detecting submicron feature size bio/chemical products such as bacteria, fungi and so on. We suggested a simple nano-filter fabrication process based on replication process. The mother pattern was fabricated by holographic lithography and reactive ion etching process, and the replication process was carried out using polymer mold and UV-imprinting process. Finally the nano-filter is obtained after removing the replicated part of metal deposited replica. In this study, as a practical example of the suggested process, a nano-dot array was replicated to fabricate nano-filter fur bacteria sensor application.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.28 no.5
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• pp.624-631
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• 2004

In this paper, new replication techniques fur a metal microcomponent having a real 3D shape were introduced. Helical gear was selected as one of a real 3D microcomponents for this study. The helical gear, which was made of photo-curable resin, was fabricated as a master pattern by microstereolithography technology. Then, a silicone rubber mold was fabricated from the master pattern. Lastly, a final bismuth alloy pattern was transferred from the silicone rubber mold by the microcasting process. In this paper, the replication technique is described in detail from the master pattern to the final pattern with some investigation on factors related to the technique.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.3
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• pp.307-314
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• 2015

In this paper, the embedding type novel liquid metal strain gauge was developed for measuring the deformation of wind turbine blades. In general, the conventional methods for the SHM have many disadvantages such as frequency distortion in FBG sensors, the low gauge factor and mechanical failures in strain gauges and extremely sophisticated filtering in AE sensors. However, the liquid metal filled in a pre-confined micro channel shows dramatic characteristics such as high sensitivity, flexibility and robustnes! s to environment. To adopt such a high feasibility of the liquid metal in flexible sensor applications, the EGaIn was introduced to make flexible liquid metal strain gauges for the SHM. A micro channeled flexible film fabricated by the several MEMS processes and the PDMS replication was filled with EGaIn and wire-connected. Lots of experiments were conducted to investigate the performance of the developed strain gauges and verify the feasibility to the actual wind turbine blades health monitoring.

• Journal of Technologic Dentistry
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• v.42 no.3
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• pp.234-239
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• 2020

Purpose: We investigated the marginal fit between abutment and metal copings according to impression technique, wax block types, and metal types. Methods: We selected the traditional impression method of using rubber impression materials and the digital impression method of using oral scanners, three types of wax blocks, and two types of metal, both of which were domestically and commercially available, were selected to produce metal copings, and the marginal fit was determined through the use of silicon replication. Results: The measurements of axial wall fit revealed that the IYV specimens had the best fit, with a mean gap of 24.11±5.95 ㎛, followed by CEV, CHV, CSS, CSV, CES, CHS, and IYS specimens (mean: 33.44±8.41 ㎛). The differences were not statistically significant. The marginal gap measurements showed that the CEV specimen had the smallest gap, 17.25±4.13 ㎛, followed by the CSV, CHV, CSS, CES, CHS, IYV, and IYS specimen (mean: 43.47±15.63 ㎛). The differences were statistically significant. Conclusion: The axial wall fit of the metal coping (VeraBond2V; Aalba Dent, Inc., Fairfield, CA, USA) produced by the lost wax technique with the traditional impression method was excellent. The marginal fit of the metal coping (VeraBond 2V) produced by wax milling with the use of an oral scanner was also excellent. The marginal fit of the metal coping was within the clinically acceptable limits in all groups.

• Polymer(Korea)
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• v.28 no.4
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• pp.305-313
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• 2004

A vacuum pressure-difference technique for making a nano-precision replica is investigated for various applications. Master patterns for replication were fabricated using a nano-replication printing (nRP) process. In the nRP process, any picture and pattern can be replicated from a bitmap figure file in the range of several micrometers with resolution of 200nm. A liquid-state monomer is solidified by two-photon absorption (TPA) induced by a femto-second laser according to a voxel matrix scanning. After polymerization, the remaining monomers were removed simply by using ethanol droplets. And then, a gold metal layer of about 30nm thickness was deposited on the fabricated master patterns prior to polydimethylsiloxane molding for preventing bonding between the master and the polydimethylsiloxane mold. A few gold particles attached on the polydimethylsiloxane stamp during detaching process were removed by a gold selecting etchant. After fabricating the polydimethylsiloxane mold, a nano-precision polydimethylsiloxane replica was reproduced. More precise replica was produced by the vacuum pressure-difference technique that is proposed in this paper. Through this study, direct patterning on a glass plate, replicating a polydimethylsiloxane mold, and reproducing polydimethylsiloxane replica are demonstrated with a vacuum pressure-difference technique for various micro/nano-applications.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.34 no.4
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• pp.262-268
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• 2021

For the past several decades, various next-generation patterning methods have been developed to obtain well-designed nano-to-micro structures, such as imprint lithography, nanotransfer printing (nTP), directed self-assembly (DSA), E-beam lithography, and so on. Especially, nTP process has much attention due to its low processing cost, short processing time, and good compatibility with other patterning techniques in achieving the formation of high-resolution functional patterns. To transfer functional patterns onto desirable substrates, the use of soft materials is required for precise replication of master mold. Here, we introduce a simple and practical nTP method to create highly ordered structures using various polymeric replica materials. We found that polymethyl methacrylate (PMMA), polystyrene (PS), and polyvinylpyridine (PVP) are possible candidates for replica materials for reliable duplication of Si master mold based on systematic analysis of pattern visualization. Furthermore, we successfully obtained well-defined metal and oxide nanostructures with functionality on target substrates by using replica patterns, through deposition and transfer process. We expect that the several candidates of replica materials can be exploited for effective nanofabrication of complex electronic devices.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.245-245
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• 2017

Heavy metal pollution of agricultural soils affects land productivity and has impact on the quality of surrounding ecosystem. Soil microbial community parameters are used as reliable indices for assessing quality of agricultural lands under metal stress. This study investigated bacterial community structure of polluted and undisturbed paddy soils to elucidate soil factors that are related to alteration of bacterial communities under conditions of metal pollution. No obvious differences in the richness or diversity of bacterial communities were observed between samples from polluted and control areas. The bacterial communities of three locations were distinct from one another, and each location possessed distinctive set of bacterial phylotypes. The abundances of several phyla and genera differed significantly between study locations. Variation of bacterial community was mostly related to soil general properties at phylum level while at finer taxonomic levels concentrations of arsenic and lead were significant factors. According to results of bacterial community functional prediction, the soil bacterial communities of metal polluted locations were characterized by more abundant DNA replication and repair, translation, transcription and nucleotide metabolism pathway enzymes while amino acid and lipid metabolism as well as xenobiotic biodegradation potential was reduced.Our results suggest that the soil microbial communities had adapted to the elevated metal concentrations in the polluted soils as evidenced by changes in relative abundances of particular groups of microorganisms at different taxonomic resolution levels, and by altered functional potential of the microbial communities.

• Journal of Powder Materials
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• v.9 no.4
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• pp.267-272
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• 2002

The production of micro components is one of the leading technologies in the fields of information and communiation, medical and biotechnology, and micro sensor and micro actuator system. Microfabrication (micromachining) techniques such as X-ray lithography, electroforming, micromolding and excimer laser ablation are used for the production of micro components out of silicon, polymer and a limited number of pure metals or binary alloys. However, since the first development of microfabrication technologies there have been demands for the cost-effective replication in large scale series as well as the extended range of available material. One such promising process is micro powder injection molding (PIM), which inherits the advantages of the conventional PIM technology, such as low production cost, shape complexity, applicability to many materials, applicability to many materials, and good tolerance. This paper reports on a fundamental investigation of the application of W-Cu powder to micro metal injection molding (MIM), especially in view of achieving a good filling and a safe removal of a micro mold conducted in the experiment. It is absolutely legitimate and meaningful, at the present state of the technique, to continue developing the micro MIM towards production processes for micro components.

• Journal of Microbiology and Biotechnology
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• v.34 no.7
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• pp.1425-1432
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• 2024

This study analyzed the effects of Ca²⁺ metal ions among culture medium components on the Chlorella sorokiniana strain DSCG150 strain cell growth. The C. sorokiniana strain DSCG150 grew based on a multiple fission cell cycle and growth became stagnant in the absence of metal ions in the medium, particularly Ca²⁺. Flow cytometry and confocal microscopic image analysis results showed that in the absence of Ca²⁺, cell growth became stagnant as the cells accumulated into four autospores and could not transform into daughter cells. Genetic analysis showed that the absence of Ca²⁺ caused upregulation of calmodulin (calA) and cell division control protein 2 (CDC2_1) genes, and downregulation of origin of replication complex subunit 6 (ORC6) and dual specificity protein phosphatase CDC14A (CDC14A) genes. Analysis of gene expression patterns by qRT-PCR showed that the absence of Ca²⁺ did not affect cell cycle progression up to 4n autospore, but it inhibited Chlorella cell fission (liberation of autospores). The addition of Ca²⁺ to cells cultivated in the absence of Ca²⁺ resulted in an increase in n cell population, leading to the resumption of C. sorokiniana growth. These findings suggest that Ca²⁺ plays a crucial role in the fission process in Chlorella.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.12
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• pp.1194-1199
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• 2007

A unique fabrication method for a copper micromesh is proposed and demonstrated. A PDMS mold was fabricated using a microcasting process and then used as a flexible mold in copper electroplating. The fabricated copper micromesh was well formed and connected without any cracks within the entire mold area. The experimental results verified that the fabricated features of the copper micromesh accurately followed the shape of the microstructures of the PDMS mold. This unique fabrication method provides an easy yet precise means of producing three-dimensional metal microstructures.