• Journal of Sensor Science and Technology
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• v.13 no.2
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• pp.90-95
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• 2004

Protein and gene detection have been growing importance in medical diagnostics. Field effect transistor (FET) - type biosensors have many advantages such as miniaturization, standardization, and mass-production. In this work, we have fabricated metal-oxide-semiconductor (MOS) FET that operates as molecular recognitions based electronic sensor. Measurements were taken with the devices under phosphate buffered saline solution. The drain current ($I_{D}$) was decreased after forming self-assembled mono-layers (SAMs) used to capture the protein, which resulted from the negative charges of SAMs, and increased after forming protein by 11.5% at $V_{G}$ = 0 V due to the positive charges of protein.

• Journal of Technologic Dentistry
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• v.34 no.4
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• pp.361-368
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• 2012

Purpose: The aim of this study was to analysis the composition on the centrifugal casting and the oxide on the casting temperature. Methods: The nickel based alloy were used in this study. Wax pattern specimens (10*10*2) were invested with phosphate-bonded investment in metal rings, the liquid/powder ratio and overall burn-out schedules for these investments were followed in accordance with the manufacturer's instructions. After casting, the alloy specimens were evaluated as regards composition(EPMA). The casting temperatures were as follows: $1400^{\circ}C$ and $1700^{\circ}C$. The quantitative analysis of oxides were scanning electron microscope(SEM), energy dispersive spectroscopy(EDS) and line scanning. Results: Nearer the injection lines showed that there is a large amount of nickel. Quantitative of oxides of Ni-Cr alloy cast from $1400^{\circ}C$ is lager than Ni-Cr alloy cast from $1700^{\circ}C$. Conclusion: Casting when using a centrifugal casting machine centrifugal force affects the composition of the alloy. The higher the temperature, the amount of oxide that is generated many.

• BMB Reports
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• v.31 no.2
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• pp.130-134
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• 1998

An aldolase gene has been cloned from Methanococcus jannaschii. The coding region of the gene has been expressed in E. coli using a pET system to a level of 30% of total cellular proteins. The protein was purified to more than 95 % homogeneity by heat treatment and ion exchange chromatography. The protein performed an aldol condensation reaction with glyceraldehyde as substrate and dihydroxyacetone phosphate as a carboxyl donor. The protein was determined to be a type II aldolase which requires the $Zn^{2+}$ ion as a metal cofactor. This enzyme has a broad range of optimum pH (7-9) and temperature ($50-80^{\circ}C$). It shows strong stability against heat, chemical denaturants, as well as a high percentage' of organic solvents. The half-life of this enzyme at $85^{\circ}C$ is more than 24 h and it maintains more than 90% of aldolase activity in the presence of 6 M urea, 50% acetonitrile, or 15% isopropyl alcohol.

• Bulletin of the Korean Chemical Society
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• v.24 no.5
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• pp.547-551
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• 2003

In the present work, the interaction of three water soluble porphyrins, tetra(p-trimethyle) ammonium phenyl porphyrin iodide (TAPP) as a cationic porphyrin, tetra sodium meso-tetrakis (p-sulphonato phenyle) porphyrin (TSPP) as an anionic porphyrin and manganese tetrakis (p-sulphonato phenyl) porphinato acetate (MnTSPP) as a metal porphyrin, with histone H₂B have been studied by isothermal titration microcalorimetry at 8 mM phosphate buffer, pH 6.8 and 27 °C. The values of binding constant, entropy, enthalpy and Gibbs free energy changes for binding of the first MnTSPP, and first and second TSPP and TAPP molecules were estimated from microcalorimetric data analysis. The results represent that the process is both entropy and enthalpy driven and histone induces self-aggregation of the porphyrins. The results indicate that both columbic and hydrophobic interactions act as self-aggregation driving forces for the formation of aggregates around histone.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.368-374
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• 1988

Enzymological proprties of an extracellular cytosine deaminase from Bacellus polymyxa YL 38-3 were investigated. The extracellular enzyme was very stable, and optimum pH and temperature for the enzyme activity were found to be near pH 6.0 in 0.2M potassium phosphate buffer and at $30^{\circ}C$, respectively. 5-Fluorocytosine was converyed to 5-fluorouracil by the enzyme, but 5-methylcytosine was not to thymine by it. The enzyme activity was completely inhibited by some heavy metal ion such as 1mM of $Cd^{2-}$ and $Hg^{2+}$, and by 1mM of p-chloromercuribenzoate, respectively. The enzyme activity was inactivated about 75% by 1mM of o-phenanthroline and monoiodoacetate. But the enzyme activity was stimulated up to 200% by 1mM of 2-mercaptoethanol.

• Journal of Surface Science and Engineering
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• v.49 no.3
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• pp.225-230
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• 2016

The present work was conducted to investigate the effects of NaF concentration in phosphate and silicate-containing alkaline electrolyte on the morphology, thickness, surface roughness and hardness of anodic oxide films formed on AZ31 Mg alloy by plasma electrolytic oxidation (PEO) method. The PEO films showed flat surface morphology with pores in the absence of NaF in the electrolyte, but nodular features appeared on the PEO film surface prepared in NaF-containing electrolyte. Numerous pores ranging from 1 to $20{\mu}m$ in size were observed in the PEO films and the size of pores decreased with increasing NaF concentration in the electrolyte. Surface roughness and thickness of PEO films showed increases with increasing NaF concentration. Hardness of the PEO films also increased with increasing NaF concentration. It was noticed that hardness of inner part of the PEO films is lower than that of outer part of them, irrespective of the concentration of NaF. The low hardness of PEO films was explained by the presence of a number of small size pores less than $2{\mu}m$ near the PEO film/substrate interface.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.5 no.3
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• pp.171-177
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• 2007

This study suggested a new method to stabilize molten salt wastes generated from the pyre-process for the spent fuel treatment. Using conventional sol-gel process, $SiO_2-Al_2O_3-P_2O_5$ (SAP) inorganic material that is reactive to metal chlorides were prepared. In this paper, the reactivity of SAP with the metal chlorides at $650{\sim}850$, the thermal stability of reaction products and their leach-resistance under the PCT-A test method were investigated. Alkali metal chlorides were converted into metal aluminosilicate($LixAlxSi1-_xO_{2-x}$) and metal phosphate($Li_3PO_4\;and\;Cs_2AlP_3O_{10}$) While alkali earth and rare earth chlorides were changed into only metal phosphates ($Sr_5(PO_4)_3Cl\;and\;CePO_4$). The conversion rate was about $96{\sim}99%$ at a salt waste/SAP weight ratio of 0.5 and a weight loss up to $1100^{\circ}C$ measured by thermogravimetric analysis were below 1wt%. The leach rates of Cs and Sr under the PCT-A test condition were about $10^{-2}g/m^2\;day\;and\;10^{-4}g/m^2\;day$. From these results, it could be concluded that SAP can be considered as an effective stabilizer for metal chlorides and the method using SAP will give a chance to reduce the volume of salt wasteform for the final disposal through further researches.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.262-270
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• 2014

BACKGROUND: Coal combustion bottom ash(BA) has high carbon and calcium content, and alkaline pH, which might improve nutrient cycling in soil related to microbial enzyme activities as it is used as soil amendment. However, it contains heavy metals such as copper(Cu), manganese (Mn), and zinc(Zn), which could cause heavy metals accumulation in soil. Compost might play a role that stabilize BA. The objective of this study was to evaluate effect of combined application of BA and compost as soil amendment on heavy metals concentration, enzyme activities, chemical properties, and crop yield in upland soil. METHODS AND RESULTS: BA was applied at the rate of 0, 20, 40, and 80 Mg/ha under different rate of compost application (0 and 30 Mg/ha) in radish (Raphanus sativus var) field. Combined application of BA and compost more improved chemical properties such as pH, EC, OM, total nitrogen, available phosphate, and exchangeable cations of soil than single application of BA. Water soluble Mn and Zn concentration in soil significantly decreased with increasing application rate of BA. Decrease in those metals concentration was accelerated with combined application of BA and compost. Urease and dehydrogenase activities significantly increased with increasing application rate of BA. Phosphotase activities were not affected with single application of BA but increased with combined application of BA and compost. Radish yield was not affected by application rate of BA. CONCLUSION: From the above results, combined application of BA and compost could be used as soil amendment to improve chemical properties and enzyme activities of soil without increase in heavy metal concentration and decrease in crop yield in upland soil.

• Korean Journal of Environmental Agriculture
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• v.29 no.2
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• pp.152-158
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• 2010

Biosorption is considered to be an alternative method to replace the present adsorbent systems for the treatment of metal contaminated wastewater. In this study, by-product which was abandoned from brewing factory was used to remove metal component in aqueous solution. The experimental results showed that the range of the removal efficiency is 60~91% and adsorption equilibrium was reached in about 3 hr. FT-IR and stereo microscope has been used to observe the surface conditions and changes in functional groups by calcination. At the end of elution, the amount of nitrogen and phosphorus in water was increased 11 and 7 times compare raw sample to calcinated samples. The Langmuir isotherm adequately described the adsorption of waste materials and the maximum adsorption capacity was 28.17 mg/g for Cd. The overall results suggested that waste material might can be used for biosorption of Cd.

• The korean journal of orthodontics
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• v.28 no.5 s.70
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• pp.689-698
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• 1998

The purpose of this study was to evaluate the shear bond strength of light cured glass ionomer cement to enamel surface which treated with $37\%$ phosphoric acid, $10\%$ polyacrylic acid, $1.23\%$ acidulated phosphate fluoride gel and no etching agent. To compare the shear bond strength of glass ionomer cement, light-cured composite resin and chemically-cured composite resin were empoloyed as controls. Eight experiments groups were composed. 10 specimens of each group were bonded by metal bracket by tested in universal testing machine for shear bond strength, in stereoscope for adhesive remnants index. The data were evaluated statistically by SPSS/PC+. The results were as follows. 1. Among the groups of $37\%$ phosphoric acid treated and dry and bonded with light cured glass ionomer, light cured composite resin, and chemically cured composite resin, the shear bond strength of glass ionomer group showed no significant difference to the others, but the shear bond strength of chemically cured resin showed statistically lower than that of light cured resin (p<0.05). 2. The shear bond strengths of glass ionomer cement to enamel treated group with $1.23\%$ acidulated phosphate fluoride gel and $10\%$ polyacrylic acid and $37\%$ phosphoric acid showed statistically higher than that of no etched enamel group(p<0.U). 3. In the groups of glass ionomer cement, the presence of moisture was not significantly effect to the shear bond strength (p<0.05). 4. After debonding, no etched enamel group showed less residual materials on the enamel surface than the group of enamel etched with $37\%$ Phosphoric acid.