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http://dx.doi.org/10.5012/bkcs.2003.24.5.547

An Isothermal Titration Microcalorimetric Study on the Interaction of Three Water-Soluble Porphyrins with Histone H2B  

Bordbar, A.K. (Department of Chemistry, Isfahan University)
Ghaderi, A.R. (Department of Chemistry, Isfahan University)
Safaei, E. (Department of Chemistry, Isfahan University)
Tangestaninejad, S. (Department of Chemistry, Isfahan University)
Eslami, A. (Department of Chemistry, University of Mazandaran)
Saboury, A.A. (Institute of Biochemistry and Biophysics, University of Tehran)
Moosavi Movahedi, A.A. (Institute of Biochemistry and Biophysics, University of Tehran)
Publication Information
Bulletin of the Korean Chemical Society / v.24, no.5, 2003 , pp. 547-551 More about this Journal
Abstract
In the present work, the interaction of three water soluble porphyrins, tetra(p-trimethyle) ammonium phenyl porphyrin iodide (TAPP) as a cationic porphyrin, tetra sodium meso-tetrakis (p-sulphonato phenyle) porphyrin (TSPP) as an anionic porphyrin and manganese tetrakis (p-sulphonato phenyl) porphinato acetate (MnTSPP) as a metal porphyrin, with histone H₂B have been studied by isothermal titration microcalorimetry at 8 mM phosphate buffer, pH 6.8 and 27 °C. The values of binding constant, entropy, enthalpy and Gibbs free energy changes for binding of the first MnTSPP, and first and second TSPP and TAPP molecules were estimated from microcalorimetric data analysis. The results represent that the process is both entropy and enthalpy driven and histone induces self-aggregation of the porphyrins. The results indicate that both columbic and hydrophobic interactions act as self-aggregation driving forces for the formation of aggregates around histone.
Keywords
Porphyrin; Histone (H₂B); Isothermal titration microcalorimetry; Aggregation;
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