• 한국어병학회지
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• 제18권2호
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• pp.179-185
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• 2005

과망간산칼륨 ($KMnO_4$), 안정화이산화염소 ($S-ClO_2$, 3% 수용액), 포르말린 (37% formaldehyde), 황산동 ($CuSO_4$)을 이용하여 넙치 적혈구의 cell damage에 미치는 효과를 알아보고자 in vitro 용혈작용과 methemoglobin (MetHb) 생성을 조사하였다. 실험의 농도는 과망간산칼륨 2-250 ppm, 안정화이산화염소 3.13-400 ppm, 포르말린 31.3-2,000 ppm, 황산동 0.04-5 ppm 이었다. 과망간산칼륨은 31.3-250 ppm, 황산동은 0.63 -5 ppm에서 현저한 용혈작용을 나타내었으나 이와는 반대로 MetHb 생성은 전혀 없었다. 따라서 과망간산칼륨과 황산동은 넙치 적혈구에 대한 cell damage system이 매우 유사한 것으로 생각되었다. 안정화이산화염소의 경우, 용혈작용은 25 ppm 이상의 농도에서 그리고 MetHb 생성은 6.25 ppm 이상의 농도에서 높게 증가하였다. 본 실험에서 유일하게 용혈작용과 MetHb 생성을 양쪽 다 현저하게 나타내었다. 포르말린은 2,000 ppm의 고농도에서도 용혈작용은 없었으며, MetHb 생성은 250-2,000 ppm에서 완만하게 증가하였다. 따라서 안정화이산화염소와 포르말린은 넙치 적혈구에 대하여 과망간산칼륨 및 황산동과는 또 다른 cell damage system을 가지고 있는 것으로 나타났다.

• 한국수산과학회지
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• 제47권6호
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• pp.860-868
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• 2014

The study was performed to investigate changes in hematological responses and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT) of Japanese eel Anguilla japonica following exposure to 0 (control), 2.33, 4.60, 6,64 and 8.78 mM nitrite-N in fresh water for 48 h. Hematological parameters such as plasma nitrite, electrolytes, cortisol, glucose, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), hemoglobin (Hb), methemoglobin (metHb) and NADH-methemoglobin reductase (NMR) were measured. Plasma nitrite, cortisol, metHb and NMR increased directly with increasing ambient nitrite concentration, while Hb content showed a progressive decline. Levels of plasma potassium, GOT and GPT of the eel exposed to 6.64 mM ambient nitrite were significantly higher than the control fish. The activity of SOD and CAT in plasma, gill and liver of the eel following exposure to nitrite were augmented by increasing ambient nitrite. Levels of plasma nitrite, metHb, NMR, cortisol, glucose and antioxidative enzyme activities of the eel exposed to 2.33 mM ambient nitrite were significantly higher than the control fish. This study suggested that the eel acutely exposed to elevated ambient nitrite causes nitrite-induced stress responses, changes in antioxidative enzyme activities and hematological parameters.

• Nutrition Research and Practice
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• 제13권2호
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• pp.105-114
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• 2019

BACKGROUND/OBJECTIVES: Several previous studies have investigated whether regular walnut consumption positively changes heart-health-related parameters. The aim of this study was to investigate the effects of daily walnut intake on metabolic syndrome (MetS) status and other metabolic parameters among subjects with MetS. SUBJECTS/METHODS: This study was a two-arm, randomized, controlled crossover study with 16 weeks of each intervention (45 g of walnuts or iso-caloric white bread) with a 6 week washout period between interventions. Korean adults with MetS (n = 119) were randomly assigned to one of two sequences; 84 subjects completed the trial. At each clinic visit (at 0, 16, 22, and 38 weeks), MetS components, metabolic parameters including lipid profile, hemoglobin A1c (HbA1c), adiponectin, leptin, and apolipoprotein B, as well as anthropometric and bioimpedance data were obtained. RESULTS: Daily walnut consumption for 16 weeks improved MetS status, resulting in 28.6%-52.8% reversion rates for individual MetS components and 51.2% of participants with MetS at baseline reverted to a normal status after the walnut intervention. Significant improvements after walnut intake, compared to control intervention, in high-density lipoprotein cholesterol (HDL-C) (P = 0.028), fasting glucose (P = 0.013), HbA1c (P = 0.021), and adiponectin (P = 0.019) were observed after adjustment for gender, age, body mass index, and sequence using a linear mixed model. CONCLUSION: A dietary supplement of 45 g of walnuts for 16 weeks favorably changed MetS status by increasing the concentration of HDL-C and decreasing fasting glucose level. Furthermore, consuming walnuts on a daily basis changed HbA1c and circulating adiponectin levels among the subjects with MetS. This trial is registered at ClinicalTrials.gov as NCT03267901.

• 분석과학
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• 제9권4호
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• pp.416-419
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• 1996

We performed a comparative study of analysis methods of Methemoglobin with a CO-Oximeter and an UV/Vis(SATO's Methd). The correlation coefficient of two method for the MetHb% determinations was estimated to be 0.9575. The concentrations of MetHb on most of the dead burn victims(87%) were shown below 10%. The concentrations of COHb were rang between 15% and 85%.

• 한국임상수의학회지
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• 제18권3호
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• pp.232-236
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• 2001

It has been known that garlic, one of the essential ingredient in korean food, has a hypotensive effect. and it is reported that they lower the level of triglycerides, cholesterol and glucose in blood. Especially, the sulfur containing amine acid and the derivatives of the garlic has the counteracting effect to heavy metals. Nowadays, the garlic is known for its efficiency for the various kinds of cancer, neoplasms, hypertension, arteriosclerosis and apoplexy. But, it is reported that the intake of the excessive amount of garlic causes hemolytic anemia recently. The hemolytic anemia is more severe especially in HK phenotype dogs which has a Na-K-ATPase activity. Therefore, this study was performed to examine the effect on the blood of the HK phenotype Jindo dogs when administered the excessive amount of garlic. HK phenotype group showed the significant decrease on RBC, WBC, PCV, Hb, MCV, MCHC, GSH, Met-Hb but LK phenotype group didn't show the significant decrease.

• 한국임상수의학회지
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• 제18권4호
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• pp.334-340
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• 2001

This study was conducted to investigate changes in blood properties of high potassium (HK) phenotype Jindo dogs (15kg$\pm$2kg) after daily oral administration with water celery extracts (10 ml/kg) for 7 days. Blood samples were collected for three days in a row before administration of water celery extracts. After water celery extracts administration, blood samples were collected at 3h, 6h, 9h and then on daily basis until day 10 post administration (PA). At day 15, final sample was collected. Blood samples were analyzed on the basis of red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), hemoglobin (Hb) concentration, mean corpuscular volume(MCV), mean corpuscular hemoglobin concentration(MCHC), gluthathione concentration(GSH) and met-hemoglobin(Met-Hb) concentration. The significant changes (p<0.01, p<0.05) of RBCs were shown at 3 h to day 5, and days 7 and 9 after administration. PCV values were decreased form 3 h to day 10 after administration. Mean Hb concentration showed significant increase as 3 h to day 3, and day 6 to day 9 after administration. The significant changes (p<0.05) of WBCs were shown at 9 h and day 1 after administration. The increased numbers of MCV were detected at days 6 to 9 after administration (p<0.05, p<0.01). The significant changes of MCHC were shown at 9h and day 1 after administration. The significant increases (p<0.01, p<0.05) of GSH concentration were detected at days 1, 6 and 7 after administration. In Met-Hb concentration, the significant increases (p<0.05) occurred at only 9h and day 7 after administration, The significant increases (p<0.01, p<0.05) of reticulocyte were detected at days 2, 4, 5, 6 and 7. Data from blood samples collected at day 15 after administration showed that all of blood analysis results returned to normal level, compared to controls.

• 한국임상수의학회지
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• 제18권3호
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• pp.237-242
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• 2001

The garlic has been eaten widely regardless of easten and westen countries to cure the various disease like cancer, tuberculosis, dentalgia, toxemia and leprosy from ancient times. Even now it is reported that they lower the level of triglycerides and cholesterol in blood and that they also affect on the cohesive power of the platelets. In addition, it is also known that it lowers the glucose level in blood. Especially, the sulfur containing amine acid and the derivatives of the garlic has the counteracting effect to heavy metals. Nowadays, the garlic is known for its efficiency for the various kinds of cancer, neoplasms, hypertension, arteriosclerosis and apoplexy. However, it is reported that the intake of the excessive amount of garlic causes hemolytic anemia recently. The hemolytic anemia is more severe especially in HK phenotype dogs which has Na-K-ATPase activity. Therefore, this study was performed to examine the effect on the blood of the HK phenotype Jindo dogs when administered the excessive amount of garlic. HK phenotype group showed the significant decrease on RBC, WBC, PCV, Hb, MCV, MCHC, GSH, Met-Hb but LK phenotype group didn's show the significant decrease. AST, ALT, BUN, creatinine, CPK, glucose, and total protein values were within normal ranges during the period.

• 대한통합의학회지
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• 제7권3호
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• pp.127-139
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• 2019

Purpose : The lipid profile is a major predictive index for cardiovascular disease, but there have only been a few studies conducted on the relationship between lipid ratio and cardiovascular disease risk factors in the Korean population. To address this research gap, we investigated the association between three lipid ratios and cardiovascular disease risk factors among Korean adults. Methods : This study used data from the seventh Korea National Health and Nutrition Examination Survey conducted in 2016. Lipid ratios included triglyceride (TG)/high density lipoprotein cholesterol (HDL), total cholesterol (TC)/HDL, and low density lipoprotein cholesterol (LDL)/HDL. Cardiovascular risk factors included in this research were: systolic blood pressure $(SBP){\geq}140$, diastolic blood pressure $(DBP){\geq}90$, fasting blood sugar $(FBS){\geq}126$, $HbA1c{\geq}6.5$, body mass index $(BMI){\geq}25$, waist circumference (WC) $men{\geq}90$, $women{\geq}85$, and metabolic syndrome (MetS). A complex samples logistic regression test was performed to analyze the association between lipid ratios and cardiovascular disease risk factors. Results : 1) TG/HDL ratio had statistically significant relationships with DBP, FBS, HbA1c, BMI, WC and MetS. 2) TC/HDL ratio was correlated to SBP, DBP, FBS, BMI, WC, and MetS. 3) LDL/HDL ratio had association with BMI, WC, and MetS. Conclusion : We identified significant association between lipid ratios and cardiovascular disease risk factors. The three lipid ratios were particularly strongly associated with BMI, WC, and MetS.

• 보건의료생명과학 논문지
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• 제9권1호
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• pp.161-167
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• 2021

본 연구는 한국 여성에서 혈청 ferritin과 25-hydroxyvitamin D [25 (OH) D]와 대사 증후군 (MetS)의 관련성을 평가하기 위해 시행되었다. 다섯 번째 국민 건강 영양 조사 (KNHANES V) (2,010- 2,012)에서 ≥ 20세 이상 성인 9,256명(4,196 폐경 전 여성과 폐경 후 여성 4,340)의 데이터를 분석 하였다. 주요 연구 결과는 다음과 같다. 첫째, MetS가 없는 여성의 경우 관련 변수 (연령, 흡연, 음주, 규칙적인 운동, SBP, DBP, WM, TC, TG, HDL-C, FPG, Hb, Hct)를 조정 한 후, 비타민 D 상태는 혈청 ferritin 수치와 관련이 있었다 (폐경 전, p <0.001, 폐경 후, p = 0.027). 둘째, MetS를 가진 여성에서 비타민 D 상태는 혈청 ferritin 수치와 관련이 없었다 (폐경 전, p = 0.739, 폐경 후, p = 0.278). 결론적으로, 비타민 D 상태는 MetS가 없는 여성에서는 혈청 ferritin 수준과 양의 상관 관계가 있었지만 MetS가 있는 여성에서는 그렇지 않았다.

• 한국어병학회지
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• 제7권2호
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• pp.161-171
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• 1994

뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다.