Journal of fish pathology (한국어병학회지)
- Volume 7 Issue 2
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- Pages.161-171
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- 1994
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
An investigation of the congestion on the gills of eel
뱀장어 아기미 울혈증에 관한 연구
- Kim, Young-Gill (Department of Fish Pathology, College of Ocean Science and Technology, Kunsan National University) ;
- Choi, Min-Soon (Department of Fish Pathology, College of Ocean Science and Technology, Kunsan National University) ;
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Park, Sung-Woo
(Department of Fish Pathology, College of Ocean Science and Technology, Kunsan National University) ;
- Lee, Keun-Kwang (Department of Fish Pathology, College of Ocean Science and Technology, Kunsan National University)
- Published : 1994.12.30
Abstract
In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities(stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit(Ht), haemoglobin(Hb), total protein(Tp), albumin(Alb), glucose(Glu), magnesium(Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase(GPT), glutamic oxalacetic transminase(GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.
뱀장어의 울혈증 발병 원인구명을 위하여 서해안 지역 5 개 양만장을 대상으로 아가미 울혈증 발병현황과 수질등 역학조사를 실시하였고, 한편으로는 스트레스(handling 과 수온차) 및 병어에서 분리한 아가미 마쇄액을 건강어의 복강에 주사하여 울혈증 발병률과 CHSE-214 에 접종하여 세포변성을 조사하였다. 조사한 양만장의 아가미 울혈증 발병률은 30-80%로서 사육시의 수온과 선별시의 수온차가 클수록 발병률이 높았으며, 수질환경과 본증의 발병과는 뚜렷한 상관관계가 없었다. 또한 발병률이 높은 양만장의 뱀장어는 Ht, Hb, Tp, Alb, Mg 및 Glu 치등이 낮은 반면 GOT, GPT, Ca 및 Met-Hb 치등은 높게 나타나는 경향을 보였으나, 반드시 아가미 울혈증에 비례하여 증감되지는 않았다. 스트레스(handling 과 수온차)를 가함에 따라 아가미 울혈증이 실험적으로 유발되어졌으며, 발병률은 50-70% 이었다. 이러한 스트레스 조건하의 어류의 혈액학적인 성상은 울혈증 발병빈도에 비례하여 Ht, GOT, GPT, Met-Hb, Alb 및 Glu 치등은 증가되었으나, Tp, Hb 및 Mg 은 감소하는 경향을 보였으며, 온도차가 클수록 cortisol 량이 증가되는 경향을 보였다. 그러나, 병어의 아가미 마쇄액을 복강주사 및 CHSE-214 에 접종하였던 바 각각 울혈증의 발병 및 세포변성이 전혀 관찰되지 않았다. 이러한 결과는 선별시 handling 과 수온의 급변이 혈중 cortisol을 상승시켜 혈액의 전해질 변화를 초래케하므로써 혈액의 흐름을 완만하게 되고 동정맥계로의 혈액유입량이 증가하게 되어 중심정맥동이 확장되므로써 울혈이 형성되는 것임을 시사한다.