• Electronics and Telecommunications Trends
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• v.39 no.4
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• pp.54-62
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• 2024

The emergence and resurgence of novel respiratory infectious diseases since the turn of the millennium, including SARS, H1N1 flu, MERS, and COVID-19, have posed a significant global health threat. Efforts to combat these threats have involved various approaches, however, continued research and development are crucial to prepare for the possibility of emerging viruses and viral variants. Direct detection methods for viral pathogens include molecular diagnostic techniques and immunodiagnostic methods, while indirect diagnostic methods involve detecting changes in the condition of infected patients through imaging diagnostics, gas analysis, and biosignal measurement. Molecular diagnostic techniques, utilizing advanced technologies such as gene editing, are being developed to enable faster detection than traditional PCR methods, and research is underway to improve the efficiency of diagnostic devices. Diagnostic technologies for infectious diseases continue to evolve, and several key trends are expected to emerge in the future. Automation will facilitate widespread adoption of rapid and accurate diagnostics, portable diagnostic devices will enable immediate on-site diagnosis by healthcare professionals, and advancements in AI-based deep learning diagnostic models will enhance diagnostic accuracy.

• Molecules and Cells
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• v.40 no.4
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• pp.262-270
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• 2017

MicroRNAs (miRNAs) are single-stranded, small RNAs (21-23 nucleotides) that function in gene silencing and translational inhibition via the RNA interference mechanism. Most miRNAs originate from host genomic regions, such as intergenic regions, introns, exons, and transposable elements (TEs). Here, we focused on the palindromic structure of medium reiteration frequencies (MERs), which are similar to precursor miRNAs. Five MER consensus sequences (MER5A1, MER53, MER81, MER91C, and MER117) were matched with paralogous transcripts predicted to be precursor miRNAs in the horse genome (equCab2) and located in either intergenic regions or introns. The MER5A1, MER53, and MER91C sequences obtained from RepeatMasker were matched with the eca-miR-544b, eca-miR-1302, and eca-miR-652 precursor sequences derived from Ensembl transcript database, respectively. Each precursor form was anticipated to yield two mature forms, and we confirmed miRNA expression in six different tissues (cerebrum, cerebellum, lung, spleen, adrenal gland, and duodenum) of one thoroughbred horse. MER5A1-derived miRNAs generally showed significantly higher expression in the lung than in other tissues. MER91C-derived miRNA-5p also showed significantly higher expression in the duodenum than in other tissues (cerebellum, lung, spleen, and adrenal gland). The MER117-overlapped expressed sequence tag generated polycistronic miRNAs, which showed higher expression in the duodenum than other tissues. These data indicate that horse MER transposons encode miRNAs that are expressed in several tissues and are thought to have biological functions.

• The Journal of the Convergence on Culture Technology
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• v.4 no.3
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• pp.269-274
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• 2018

This study was done to identify the effects of the hand washing procedure poster on the hand washing behaviors after Mers outbreak. Method: This study was conducted by observing a total of 370 people(185 people in no- poster zone, 185 people in poster zone) who used the public restroom. Results: As a result of comparing the hand washing with and without the poster, the subjects who washed their hands with the poster around were 83.2%, which was higher than the 47% of the subjects without the poster. The Time spent on the hand washing was similar between the groups with or without the poster around. Also only 3.3% of the subjects actually completed the entire 6 procedures of hand washing. The subjects who finished more than 4 procedures out of 6 were 17% from the group with the poster around, and 10.3% from the group without. Conclusion: The hand washing poster was effective in encouraging the behavior itself, but not specifically in the right way during the ideal time nor the right procedures. Therefore, verbal instructions made every time washing hands or more visual effective posters are required to enhance the hand washing procedures.

• Journal of Mushroom
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• v.14 no.4
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• pp.174-178
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• 2016

Simple sequence repeats (SSR), also referred to "microsatellites" consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from 'Heuktari' and 'Miso', which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of 'Heuktari' and 'Miso' were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the 'Heuktari' and 'Miso' genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in 'Heuktari' and 'Miso' were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

• The Journal of Bigdata
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• v.2 no.1
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• pp.59-69
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• 2017

According to precedent research of disaster economics, most of the studies are either based on belated macroeconomic indicators or are limited to specific industries. It is certain that preventing disaster is important, but immediate analysis and reconstruction policy are crucial as well. This research analyzed the ripple effect of consumer spending followed by April 16 ferry disaster and MERS outbreak; it was done by applying credit card company's real-time big data with Marketing Mix Modeling. The main focus of this research is to see if it is possible to predict the scale of damage during ongoing disasters. It is found that setting up weekly MMM and moving the timeline draws significance conclusion. When disasters or events occur in future, this research may be the basis of building quick and intuitive indicator to monitor possible effects.

• KSBB Journal
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• v.14 no.6
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• pp.639-642
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• 1999

Optimization of the chitosanoligosaccharide production was studied with chitosanase. The optimum conditions for the enzymic reaction have been determined. Enzyme stability was maintained above 90% after 6 days at pH 5.0. The optimum initial reaction rate was appeared in 1.0% of chitosan solution. The production yield of chitosanoligosaccharides was over at 0.4%~2.0% of chitosan. At 4.0% of chitosan solution, however, the production yield was decreased to 64.6%. To increase the yield, stepwise addition of substrate into the reactor was investigated. In this case, the yield was increased from 64.6% to 83.2% and the final concentrations of chitosanoligosaccharide was 12.26 mg/mL. By TLC analysis, most of the chitosanoligosaccharides produced were 3-5 mers.

• The Research Journal of the Costume Culture
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• v.28 no.3
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• pp.285-298
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• 2020

This study aimed at examining fashion consumers' awareness during the COVID-19 pandemic. Big data analysis methods, such as text mining, social network analysis, and regression analysis, were applied to user posts about fashion on Korean portal websites and social media during COVID-19. R 3.4.4, UCINET 6, and SPSS 25.0 software were used to analyze the data. The results were as follows. In researching the popular fashion-related topics during COVID-19, the prevention of infection and prophylaxis were significant concerns in the early stage (Jan 1 to Jan 31, 2020), and changed to online channels and online fashion platforms. Then, various topics and fashion keywords appeared with COVID-19-related keywords afterwards. Fashion-related subjects concerned prophylaxis, home life, digital and beauty products, online channels, and fashion consumption. In comparing fashion consumers' awareness during COVID-19 with SARS and MERS, "face masks" was the common keyword for all three illnesses; yet, the prevention of infection was a major consumer concern in fashion-related subjects during COVD-19 only. As COVD-19 cases increased, the search volume for face masks, shoes, and home clothes also increased. Consumer awareness about face masks shifted from blocking yellow dust and micro-dust to the sociocultural significance and short supply. Keywords related to performance turned out to be the major awareness as to shoes, and home clothes were repurposed with an expanded range of use.

• Journal of Digital Convergence
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• v.15 no.3
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• pp.287-294
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• 2017

This study attempted to develop a beacon scanner based ward monitoring service in order to respond to the new paradigm of medical environment which is trying to introduce ICT technology as medical service to track and manage the spread path of large infectious diseases such as MERS. The study also included beacon hardware development, firmware development for the beacon low-power bluetooth 4.0, and server and web-based dashboard UI development. Using these, we have developed a customized monitoring system that provides functions such as locating patients by location based service and monitoring based on web UI. It is possible to maximize the efficiency of offline hospital services and to value active infection control and patient safety by integrating online technology into the area where online technologies such as beacons are not properly integrated.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.293-301
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• 2016

High-quality gene sets are necessary for functional research of genes. Although Perilla is a commonly cultivated oil crop and vegetable crop in Southeast Asia, the quality of its available gene set is insufficient. To construct a high-quality Perilla gene set, we sequenced mRNAs extracted from different tissues of Perilla citriodora, the wild species (2n = 20) of Perilla. To make a high-quality gene set for P. citriodora, we compared the quality of assemblies produced by Velvet and Trinity, the two well-known de novo assemblers, and improved the de novo assembly pipeline by optimizing k-mers and removing redundant sequences. We then selected representative transcripts for loci according to several criteria. The improved assembly yielded a total of 86,396 transcripts and 38,413 representative transcripts. We evaluated the assembled transcripts by comparing them to 638 homologous Arabidopsis genes involved in fatty acid and TAG biosynthesis pathways. High proportions of full-length genes and transcripts in the assembled transcripts matched known genes in other species, indicating that the P. citriodora gene set can be applied in future functional studies. Our study provides a reference P. citriodora gene set for further studies. It will serve as valuable genetic resource to elucidate the molecular basis of various metabolisms.

• Korean Journal of Microbiology
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• v.44 no.2
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• pp.164-167
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• 2008

Recently, rolling circle amplification (RCA) technique has been widely focused in the field of gene amplification just like PCR method. We have developed RCA-mer, which is a web-based program searching for primer candidates from a given sequence. It can be applied to find primer lists in DNA amplification experiment based on RCA method. The RCA-mer compares 8-mer primer lists with user's input sequence such as vector, mitochondria, and microbial genome sequence. After calculating 8-mers existences in a given sequence, it displays matched and no-matched primer lists with their GC-contents. In addition to it, RCA-mer can search the existence of 8-mer primer lists in two sequences whether they are co-existed or not. Users can apply candidate primer lists to their researches which use RCA techniques.